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The multi-attribute method directly characterizes and monitors multiple product quality attributes of a biopharmaceutical product via proteolytic digestion of the sample followed by reversed-phase chromatographic separation and high-resolution liquid chromatography-mass spectrometry analysis.
LiP-MS is an approach based on limited proteolysis that can be used to probe structural changes in proteins. This update highlights recent changes to the method to improve throughput, proteome coverage and data analysis.
A protocol for purification, full-length sequencing and genomic origin mapping of extrachromosomal circle DNA using a three-step purification strategy combined with rolling-circle amplification and Oxford Nanopore sequencing.
Mesoporous carbonaceous nanomaterials have technological potential for diverse applications ranging from gas separation to energy storage. We describe the template-directed assembly of monomicelles to make mesoporous materials with different structures.
IonStar is an integrated proteomics suite utilizing ultrahigh-resolution-MS1 acquisition to achieve high-quality quantification of large sample cohorts. IonStar encompasses strategies for sample preparation, liquid chromatography–mass spectrometry analysis and data processing.
A micro-computed X-ray tomography-based approach for quantifying the number and volume of lung cancer nodules over time, enabling the tracking of individual nodule formation, tumor growth and response to therapy.
Simultaneous electrophysiological recordings are obtained from multiple brain regions using Neuropixels probes. The procedure covers the implantation of a headframe, the targeted positioning of probes and their insertion through a perforated imaging window.
Here the authors provide a protocol for functional genomics using engineered symbionts (FUGUES) that utilizes an engineered bee gut bacterium to induce a sustained RNAi response in the host that can be used to study honey bee biology.
A protocol for precise localization of cortical muscle representations within the primary motor cortex via functional analysis of induced electric fields by transcranial magnetic stimulation and the resulting motor-evoked potentials.
This protocol details quantum dot-based single-virus tracking, an imaging approach to monitoring the infection behavior of individual viruses or viral components in live cells by using semiconductor nanoparticles (quantum dots) as fluorescent labels.
This protocol describes the preparation of high-quality single-cell suspensions from the human placenta for downstream characterization, using droplet-based single-cell RNA sequencing platforms.
Microcrystals are increasingly being used for structural analysis. The challenge addressed by this protocol is to generate large numbers of high-quality crystals of uniform size for various experiments, including time-resolved studies of reactions.
This protocol uses PlantPegDesigner to design and optimize prime editing guide RNA and engineered plant prime editor vectors for efficient prime editing in monocot plants.
The Nodewalk assay converts chromosome conformation capture DNA samples to RNA and, subsequently, cDNA to enable ultrasensitive quantitative analyses of chromatin fiber interactions.
This protocol describes N6-methyladenosine (m6A)-SAC-seq, site-specific base-resolution m6A sequencing in the whole transcriptome, including preparing the enzyme and synthesizing the allyl-SAM cofactor required for library preparation, and data analysis for m6A sites.
An approach to reproducing episodes of hypotension-mimicking orthostatic challenges while monitoring arterial blood pressure and sympathetic nerve activity over several months in preclinical models of chronic neurological disorders, using a combination of available telemetry technologies, optogenetics and neuronal tract tracing.
This protocol describes how to perform an open thoracic surgery for epicardial implantation of wireless cardiac bioelectronic devices in adult rats and the methodology for a full biocompatibility assessment of the physiological response to the implanted device.