An absolute quantification approach combined with differential affinity capture provides a means by which to accurately measure distinct pools of ubiquitin in cells or tissues.
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Bennett, E., Harper, J. Simply quantifying ubiquitin complexity. Nat Methods 8, 630–631 (2011). https://doi.org/10.1038/nmeth.1651
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DOI: https://doi.org/10.1038/nmeth.1651