Much of our knowledge about biological systems has been obtained by examining ensembles of molecules. However, this has begun to change because of the unprecedented precision and clarity afforded by single-molecule measurements. The last decade has seen amazing advances in the resolution and complexity of these methods, making it possible to ask and answer entirely new types of biological questions.
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Acknowledgements
Grants from the US National Science Foundation (PHY 1430124) and US National Institutes of Health (GM065367) supported T.H.'s single-molecule methods development efforts.
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Ha, T. Single-molecule methods leap ahead. Nat Methods 11, 1015–1018 (2014). https://doi.org/10.1038/nmeth.3107
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DOI: https://doi.org/10.1038/nmeth.3107
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