Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum

Journal name:
Nature
Volume:
480,
Pages:
534–537
Date published:
DOI:
doi:10.1038/nature10606
Received
Accepted
Published online
Corrected online

Erythrocyte invasion by Plasmodium falciparum is central to the pathogenesis of malaria. Invasion requires a series of extracellular recognition events between erythrocyte receptors and ligands on the merozoite, the invasive form of the parasite. None of the few known receptor–ligand interactions involved1, 2, 3, 4 are required in all parasite strains, indicating that the parasite is able to access multiple redundant invasion pathways5. Here, we show that we have identified a receptor–ligand pair that is essential for erythrocyte invasion in all tested P. falciparum strains. By systematically screening a library of erythrocyte proteins, we have found that the Ok blood group antigen, basigin, is a receptor for PfRh5, a parasite ligand that is essential for blood stage growth6. Erythrocyte invasion was potently inhibited by soluble basigin or by basigin knockdown, and invasion could be completely blocked using low concentrations of anti-basigin antibodies; importantly, these effects were observed across all laboratory-adapted and field strains tested. Furthermore, Oka− erythrocytes, which express a basigin variant that has a weaker binding affinity for PfRh5, had reduced invasion efficiencies. Our discovery of a cross-strain dependency on a single extracellular receptor–ligand pair for erythrocyte invasion by P. falciparum provides a focus for new anti-malarial therapies.

At a glance

Figures

  1. BSG is an erythrocyte receptor for PfRh5.
    Figure 1: BSG is an erythrocyte receptor for PfRh5.

    a, PfRh5 was screened as either a prey (top panel) or a bait (bottom panel) against an erythrocyte receptor protein library using AVEXIS. BSG (protein 9) was identified as a receptor for PfRh5 in both bait–prey orientations. b, Domain structure of the BSG isoforms (left); lollipops represent potential N-linked glycosylation sites. BSG regions were expressed as baits and used to map the PfRh5 binding site to the two membrane-proximal domains. Bar charts show mean±s.e.m.; n = 3. c, Biophysical analysis of the PfRh5–BSG-S interaction using SPR. The indicated concentrations of purified PfRh5 were injected over immobilised BSG, and biophysical parameters derived from a 1:1 binding model (red line). RU, response units.

  2. Soluble BSG, anti-BSG antibodies and BSG knockdown potently block erythrocyte invasion.
    Figure 2: Soluble BSG, anti-BSG antibodies and BSG knockdown potently block erythrocyte invasion.

    a, Erythrocyte invasion was inhibited by purified pentamerized BSG-S–Cd4d3+4–COMP–His ectodomains but not by the two non-binding BSG-S domains added individually or Cd4d3+4–COMP-His (control); strain Dd2. b, Cross-strain inhibition of invasion using pentamerized BSG-S. c, Anti-BSG monoclonal antibodies, TRA-1-85 and MEM-M6/6, potently inhibited invasion of erythrocytes; strain 3D7. d, MEM-M6/6 concentrations10µgml−1 prevented all detectable invasion by microscopic observation of cultures; strain 3D7. e, f, MEM-M6/6 inhibited invasion of synchronised P. falciparum culture-adapted lines (e) and unsynchronised field isolates (f). g, Cell-surface BSG is reduced in erythrocytes differentiated from haematopoietic stem cells transduced with lentiviruses containing shRNA targeting BSG (light blue line) relative to a control virus (pLKO, shaded); black line represents secondary antibody alone. h, 3D7 and W2mef invasion was inhibited in BSG knockdown erythrocytes. A and B are replicates. Invasion efficiencies are mean±s.e.m., n = 3.

  3. The Oka- BSG variant has reduced binding affinity for PfRh5 and Oka- erythrocytes have reduced merozoite invasion frequencies.
    Figure 3: The Oka− BSG variant has reduced binding affinity for PfRh5 and Oka− erythrocytes have reduced merozoite invasion frequencies.

    a, Schematic of the membrane distal IgSF domain of BSG-S showing the location of naturally-occurring variants. b, Equilibrium binding isotherms of PfRh5 binding to BSG-S variants. c, Association (ka) and dissociation (kd) rate constants of PfRh5 binding to BSG-S and variants. Means±s.e.m.; n = 3. d, Invasion of 3D7 and Dd2 strains in Oka− blood cells are reduced relative to the Oka+ control. Mean±s.e.m., n = 3; *P0.0003; #P = 0.0349, unpaired one-tailed t test. A repeat is shown in Supplementary Fig. 9.

Main

Among the many P. falciparum merozoite proteins that are believed to have a role in erythrocyte invasion, most attention has focused on two major parasite protein families: the EBAs and Rhs7. Although erythrocyte receptors have been identified for some of them (members of the glycophorin family are receptors for three EBAs1, 2, 3and complement receptor 1 (CD35) has recently been identified as a receptor for PfRh4, ref. 4) none of these receptor–ligand pairs are essential in all parasite strains tested. PfRh5 is unique amongst the EBAs and Rhs because it cannot be deleted in any P. falciparum strain and is therefore apparently essential for parasite growth in blood stage culture5, 6. Both native and recombinant PfRh5 have been previously shown to bind erythrocytes through an unknown glycosylated receptor that is resistant to chymotrypsin, trypsin and neuraminidase treatment6, 8, 9.

To identify an erythrocyte receptor for PfRh5, we used a systematic screening approach by first compiling a library of abundant cell surface and secreted proteins expressed by human erythrocytes based on published proteomics data10. Proteins for which the entire ectodomain was expected to be expressed as a soluble recombinant protein were selected (Supplementary Table 1), and expressed by mammalian cells (Supplementary Fig. 1). The 40 proteins within the erythrocyte ectodomain protein library were then systematically screened using the AVEXIS assay (avidity-based extracellular interaction screen)11 for interactions with a recombinant PfRh5 protein, also produced by mammalian cells. The AVEXIS assay is designed to detect direct low-affinity protein interactions between ectodomain fragments expressed as either biotin-tagged baits or highly avid pentameric β-lactamase-tagged preys12, 13. The PfRh5 prey interacted with a single erythrocyte receptor bait (Fig. 1a, top panel) corresponding to the Ok blood group antigen, basigin (BSG, also known as CD147, EMMPRIN and M6, ref. 14). The same single interaction was identified in the reciprocal bait–prey orientation (Fig. 1a, lower panel).

Figure 1: BSG is an erythrocyte receptor for PfRh5.
BSG is an erythrocyte receptor for PfRh5.

a, PfRh5 was screened as either a prey (top panel) or a bait (bottom panel) against an erythrocyte receptor protein library using AVEXIS. BSG (protein 9) was identified as a receptor for PfRh5 in both bait–prey orientations. b, Domain structure of the BSG isoforms (left); lollipops represent potential N-linked glycosylation sites. BSG regions were expressed as baits and used to map the PfRh5 binding site to the two membrane-proximal domains. Bar charts show mean±s.e.m.; n = 3. c, Biophysical analysis of the PfRh5–BSG-S interaction using SPR. The indicated concentrations of purified PfRh5 were injected over immobilised BSG, and biophysical parameters derived from a 1:1 binding model (red line). RU, response units.

BSG is a member of the immunoglobulin superfamily (IgSF) and has been implicated in many biological functions including embryo implantation, spermatogenesis15 and retinal development16. BSG exists in both long (three IgSF domains, BSG-L) and short (two IgSF domains, BSG-S) splice isoforms (Fig. 1b) and although BSG-L was used in the screen, BSG-S is thought to be the major isoform expressed on erythrocytes. Binding experiments using domain deletions established that PfRh5 could interact with BSG-S and this required both domains because neither of the two BSG-S IgSF domains were individually able to bind PfRh5 (Fig. 1b and Supplementary Fig. 2). We showed that PfRh5 interacted directly with BSG-S and BSG-L using purified proteins and surface plasmon resonance (SPR). Both kinetic (Fig. 1c) and equilibrium (Supplementary Fig. 3) binding parameters for the interaction were derived using a 1:1 binding model and were in excellent agreement (Supplementary Table 2). These parameters are typical of extracellular protein interactions measured using this technique17. Removal of glycans from BSG either by mutating all predicted glycosylation motifs or by enzymatic treatment did not affect PfRh5 binding (Supplementary Fig. 4), indicating that the PfRh5 binding site is solely located in the BSG protein core. BSG is also known to be resistant to trypsin and chymotrypsin treatment18, consistent with previous PfRh5–erythocyte binding studies6, 8, 9.

To determine whether the PfRh5–BSG interaction was required for invasion, we added purified pentamerized soluble BSG-S into invasion assays to specifically compete with the membrane-bound receptor. We found that BSG-S strongly inhibited invasion in a dose-dependent manner relative to controls which included each of the two non-binding BSG-S IgSF domains added individually (Fig. 2a). Strong inhibition was also observed across multiple strains (Fig. 2b) or when soluble BSG-L was added (Supplementary Fig. 5), although this was slightly weaker for the 3D7 strain. Soluble forms of BSG consisting of the extracellular regions are known to have biological effects such as upregulation of matrix metalloproteases19. To rule out an indirect effect of exogenous BSG on invasion, we added to invasion assays two independent purified anti-BSG monoclonal antibodies (MEM-M6/6 and TRA-1-85) which could both block the PfRh5–BSG interaction in vitro (data not shown). These high-affinity reagents gave a potent invasion blocking effect that was saturable at very low antibody concentrations (half-maximum inhibitory concentration0.5µgml−1), consistent with binding and occluding a specific surface receptor of typical abundance (~104 to 106 molecules per cell20) (Fig. 2c). Pre-adsorption of the MEM-M6/6 antibody with soluble monomeric BSG specifically relieved the inhibition, ruling out any indirect effect of the antibody on non-BSG targets; furthermore, MEM-M6/6 did not affect intra-erythrocytic P. falciparum development (Supplementary Fig. 6). Invasion was quantified using flow cytometry and a fluorescent DNA dye to stain parasites21. Using this assay, apparent invasion could not be eliminated, with efficiencies reduced to a maximum of 80–90%, even at much higher concentrations of antibody (up to 1.5mgml−1 of MEM-M6/6, data not shown); however, direct observation of parasites using Giemsa-stained thin smears revealed that this residual staining in cytometry assays was due to extracellular parasites and debris in the culture. Using microscopy-based assays, we found that MEM-M6/6 concentrations of 10µgml−1 or more was sufficient to prevent all detectable invasion (Fig. 2d).

Figure 2: Soluble BSG, anti-BSG antibodies and BSG knockdown potently block erythrocyte invasion.
Soluble BSG, anti-BSG antibodies and BSG knockdown potently block erythrocyte invasion.

a, Erythrocyte invasion was inhibited by purified pentamerized BSG-S–Cd4d3+4–COMP–His ectodomains but not by the two non-binding BSG-S domains added individually or Cd4d3+4–COMP-His (control); strain Dd2. b, Cross-strain inhibition of invasion using pentamerized BSG-S. c, Anti-BSG monoclonal antibodies, TRA-1-85 and MEM-M6/6, potently inhibited invasion of erythrocytes; strain 3D7. d, MEM-M6/6 concentrations10µgml−1 prevented all detectable invasion by microscopic observation of cultures; strain 3D7. e, f, MEM-M6/6 inhibited invasion of synchronised P. falciparum culture-adapted lines (e) and unsynchronised field isolates (f). g, Cell-surface BSG is reduced in erythrocytes differentiated from haematopoietic stem cells transduced with lentiviruses containing shRNA targeting BSG (light blue line) relative to a control virus (pLKO, shaded); black line represents secondary antibody alone. h, 3D7 and W2mef invasion was inhibited in BSG knockdown erythrocytes. A and B are replicates. Invasion efficiencies are mean±s.e.m., n = 3.

P. falciparum isolates can vary widely in their ability to invade erythrocytes treated with different receptor-modifying enzymes such as trypsin, chymotrypsin and neuraminidase, showing differential dependencies on erythrocyte receptors for invasion. To determine if BSG was a critical invasion receptor across P. falciparum lines that use different invasion pathways, we tested the ability of MEM-M6/6 to block erythrocyte invasion on nine culture-adapted strains representing seven different PfRh5 sequence variants (Supplementary Table 3). We observed that the invasion of all lines was potently inhibited by MEM-M6/6 (Fig. 2e). To show that the dependency on BSG was not an unusual feature of culture-adapted lines, we also tested six freshly-isolated P. falciparum strains from Senegal22 and again observed a potent inhibitory effect (Fig. 2f). Assays with the field isolates were carried out with unsynchronised parasites, decreasing the overall inhibitory effect because not all parasites had reinvaded over the course of the assay. All six Senegal isolates, however, were inhibited by MEM-M6/6 to the same extent as an unsynchronised culture-adapted line, W2mef, tested at the same time. This demonstrated that freshly-isolated field strains have the same dependency on BSG as laboratory-adapted lines (Fig. 2f).

To confirm independently the essentiality of BSG as a P. falciparum invasion receptor, we used a genetic approach by differentiating erythrocytes from haematopoietic stem cells transduced with lentiviruses containing either a short hairpin RNA targeting BSG or a scrambled shRNA control (pLKO). BSG-targeted erythrocytes showed a reproducible knockdown to approximately 50 to 60% of cell surface BSG levels relative to the pLKO control (Fig. 2g) and expressed markers indicative of complete erythrocyte maturation (Supplementary Fig. 7). The invasion of both the 3D7 and W2mef P. falciparum strains into BSG-knockdown erythrocytes was significantly reduced compared to the control (18% versus 94% for 3D7 and 14% versus 103% for W2mef, Fig. 2h). By contrast, previous knockdown of GYPA, the major surface sialoglycoprotein, significantly inhibited the W2mef but not the 3D7 strain23. The inhibition of erythrocyte invasion by multiple P. falciparum strains using soluble BSG, anti-BSG monoclonal antibodies, or knockdown of BSG surface expression suggests that BSG is a critical host receptor for P. falciparum invasion.

Malaria is thought to have been a strong selective pressure in human evolutionary history and given the apparently essential roles of PfRh5 and BSG in P. falciparum invasion we sought to determine if any human populations contained genetic variants in BSG that might affect PfRh5 binding and invasion. Five nonsynonymous single nucleotide polymorphisms (SNPs) have been described within the BSG-S IgSF domains (Supplementary Table 4 and Fig. 3a). These variants were expressed and the biophysical PfRh5 binding parameters determined using SPR. Equilibrium measurements showed that two variants had lower binding affinity compared to the BSG reference sequence: L90P and E92K (Fig. 3b and Supplementary Table 2). L90P did not interact with PfRh5 and binding profiles of several anti-BSG monoclonal antibodies indicated local misfolding of the membrane-distal IgSF domain (Supplementary Fig. 8). No verification or population frequency data for this SNP are currently available, preventing further biological interpretation of this variant. E92K had a twofold lower affinity for PfRh5 (Fig. 3b) and a comparative kinetic analysis demonstrated that this was due to both a slower association and a faster dissociation rate (Fig. 3c and Supplementary Table 2). The E92 residue is solvent-exposed and located within the loop connecting the F–G β-strands close to the glycan-free GFC β-sheet, consistent with a possible PfRh5-binding interface (Fig. 3a). E92K is the variant responsible for the Oka− blood group, which has been described in eight Japanese families14. Oka− erythrocytes from two unrelated donors showed reduced invasion with both 3D7 and Dd2 P. falciparum strains relative to Oka+ controls (Fig. 3d and Supplementary Fig. 9), correlating with the reduced affinity of the Oka− variant for PfRh5. The extreme rarity and restriction of the Oka− blood group to Japanese individuals suggest that this specific allele has not had a major role in conferring resistance to malaria. It is possible that other BSG polymorphisms, as yet unknown, have evolved in some malaria-exposed populations as a mechanism of resistance to P. falciparum. The search for functional polymorphisms of BSG needs to go beyond gene coding regions as the results of our knockdown experiments indicate that expression levels of BSG at the erythrocyte surface influence the ability of the parasite to invade. The Duffy variant, which confers resistance to P. vivax, is also a non-coding regulatory polymorphism that suppresses expression of the invasion receptor by erythrocytes. Our ability to address this problem is currently limited by the lack of data on genome variation among the many different ethnic groups that are exposed to P. falciparum malaria, but will be greatly enhanced by the 1000 Genomes Project, MalariaGEN and other genetic studies that are now in progress in Africa and other malaria-endemic regions of the world24, 25, 26. Inter-population comparisons of haplotype length and frequency provide a potentially powerful way of addressing this problem27, and there is preliminary evidence that a region of chromosome 19 encompassing BSG and several neighbouring genes has undergone recent positive selection in West Africa, but a considerable amount of further work is needed to determine whether this is causally related to the role of BSG as a malaria invasion receptor (MalariaGEN consortium, unpublished data).

Figure 3: The Oka− BSG variant has reduced binding affinity for PfRh5 and Oka− erythrocytes have reduced merozoite invasion frequencies.
The Oka- BSG variant has reduced binding affinity for PfRh5 and Oka- erythrocytes have reduced merozoite invasion frequencies.

a, Schematic of the membrane distal IgSF domain of BSG-S showing the location of naturally-occurring variants. b, Equilibrium binding isotherms of PfRh5 binding to BSG-S variants. c, Association (ka) and dissociation (kd) rate constants of PfRh5 binding to BSG-S and variants. Means±s.e.m.; n = 3. d, Invasion of 3D7 and Dd2 strains in Oka− blood cells are reduced relative to the Oka+ control. Mean±s.e.m., n = 3; *P0.0003; #P = 0.0349, unpaired one-tailed t test. A repeat is shown in Supplementary Fig. 9.

In summary, we have applied a systematic protein interaction screening approach (AVEXIS) to identify BSG as an erythrocyte receptor for PfRh5. Importantly, we were able to prevent all detectable erythrocyte invasion by every P. falciparum strain that we tested using only modest concentrations of anti-BSG antibodies. These observations, coupled with the inability to delete PfRh56, lead us to conclude that the interaction between BSG and PfRh5 is essential for parasite entry, and may perform a fundamentally different function to the other EBA and Rh proteins, which are involved in redundant, partially overlapping invasion pathways. The dependence on a single receptor–ligand pair across many P. falciparum strains may provide new possibilities for therapeutic intervention.

Methods

Recombinant protein production

Proteins selected for expression included all type I, type II, GPI (glycophosphatidylinositol)-linked receptors and secreted proteins. Some multipass transmembrane proteins were also included where there was an extracellular N terminus preceded by a signal peptide (Supplementary Table 1). Individual domains of human BSG were produced by identifying domain boundaries using the structure of the BSG extracellular region28, 29 and amplifying these regions using primers with flanking NotI and AscI restriction enzyme sites to facilitate cloning. The carboxy-terminal amino acid sequence of the BSG-d0+1 and BSG-d1 constructs was HGPP. BSG-d2 was cloned into the same vector as PfRh5 to add an exogenous signal peptide required for protein secretion and encompassed the sequence between PPRV.. and ..RSHL. Glycosylation sites were removed in BSG by mutating codons encoding all three asparagines in glycosylation motifs to aspartic acid. To remove N-linked glycans from soluble recombinant BSG, 500units of PNGase F (New England Biolabs) were added to 10µl of a spent tissue culture supernatant and incubated for 15min at 37°C. Sialic acid residues were removed by adding 1.6 milli-units of Vibrio cholerae neuraminidase (Sigma) to 10µl of a spent tissue culture supernatant and incubated for 15min at 37°C.

Interaction screening by AVEXIS

For the AVEXIS assay, bait and prey protein preparations were normalized to activities that have been previously shown to detect transient interactions (monomeric half-lives less than 0.1s) with a low false positive rate11. Biotinylated baits dialysed against HBS were immobilised in the wells of a streptavidin-coated 96-well microtitre plate (NUNC). Normalized preys were added, incubated for 2h at room temperature, washed three times in HBS/0.1% Tween-20, and once in HBS. 125µgml−1 of nitrocefin was added, and absorbance values measured at 485nm on a Pherastar plus (BMG laboratories). Controls were essentially as described12 and included the Cd4d3+4 tag alone as a negative control bait and a biotinylated anti-Cd4 (anti-prey) antibody as a prey capture positive control. A positive control interaction consisting of the rat Cd200 bait detected using the rat Cd200R prey used at the threshold level and both 1:10 and 1:100 dilutions was included on each plate. The negative (−) and positive (+) control interactions shown in Fig. 1a are the rat Cd200R prey used at the screening threshold probed against the Cd4d3+4 (−) or rat Cd200 (+) baits.

P. falciparum culture, characterization and invasion assays

All P. falciparum parasite strains were routinely cultured in human O+ erythrocytes at 5% haematocrit in complete medium (RPMI-1640 containing 10% human serum), under an atmosphere of 1% O2, 3% CO2 and 96% N2. To confirm their identity, laboratory-adapted strains were genotyped by PCR within polymorphic regions of the msp1 and msp2 genes30. Parasite cultures were synchronized in early stages with 5% (w/v) d-sorbitol (Sigma). Use of erythrocytes from human donors for P. falciparum culture was approved by the NHS Cambridgeshire 4 Research Ethics Committee. Oka− blood was obtained from donors in Japan with informed consent, and shipped on ice. For each sample, a control Oka+ sample was collected at the same time under identical conditions. All experiments were performed within 72h of collection.

Invasion assays were carried out in round-bottom 96-well plates, with a culture volume of 100µl per well at a haematocrit of 2%. Parasites in trophozoite stage were mixed with pentamerized BSG-S–Cd4d3+4–COMP–His ectodomains or with anti-BSG monoclonal antibodies and incubated in the plates for 24h at 37°C inside a static incubator culture chamber (VWR), gassed with 1% O2, 3% CO2 and 96% N2. At the end of the incubation period, red blood cells (RBC) were collected and parasitized RBC (pRBC) were stained with 2µM Hoechst 33342 (Invitrogen), as described previously21. Invasion assays using Oka− blood and control Oka+ blood were carried out following the two-colour flow cytometric assay described in ref. 21. Briefly, Oka− blood and control Oka+ blood were labelled with 10µM DDAO-SE (Invitrogen). RBC were resuspended to 2% haematocrit, mixed with pRBC (ring stage) and incubated in 96-well plates for 48h as described above. At the end of the incubation period, RBC were harvested and pRBC were stained with 2µM Hoechst 33342. Standard blood smear microscopy was performed to determine parasitaemia. Briefly, a small aliquot of the culture was smeared on a glass slide, fixed with 100% methanol and stained with Field’s Stain (Pro-Lab Diagnostics). Parasitaemia was determined by counting the number of parasitized pRBC per 2,000 total RBC examined by oil immersion with a Leica DME microscope (Leica Microsystems). All parasitaemia represented was the average of three replicates. Lentiviral transductions of HSCs was performed as previously described23. Lentiviral-delivered shRNA sequences were BSG; TRC clone ID (TRCN0000006736) hairpin sequence: 5′-GAAGTCGTCAGAACACATCAACTCGAGTTGATGTGTTCTGACGACTTC-3′, pLKO scrambled control; (Addgene plasmid 1864) hairpin sequence: 5′-CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG-3′; loop region indicated in bold. Detailed Standard Operating Procedures for all invasion assays are available at http://www.sanger.ac.uk/research/.

Flow cytometry

Stained samples were examined with a 355-nm ultraviolet laser (20mW) and a 633-nm red laser (17mW) on a BD LSRII flow cytometer (BD Biosciences). Hoechst 33342 (Invitrogen) was excited using the ultraviolet laser and detected with a 450/50 filter, whereas DDAO-SE (Invitrogen) was excited using the red laser and detected with a 660/20 filter. BD FACS Diva (BD Biosciences) was used to collect 100,000 events for each sample. FSC and SSC voltages of 423 and 198, respectively, and a threshold of 2,000 on FSC were applied to gate the erythrocyte population. The data collected were further analysed with FlowJo (Tree Star). All experiments were carried out in triplicate. GraphPad Prism (GraphPad Software) was used to plot the generated parasitaemia data.

PfRh5 cloning and sequencing

Total RNA was extracted from 3D7 and FCR3 schizonts using the QIAamp RNA Blood Mini Kit (Qiagen). Isolated RNA was treated with TURBO DNase (Ambion) and reverse transcribed to cDNA using the High-Capacity cDNA Archive Kit (Applied Biosystems) following the manufacturer’s instructions. A 10µl aliquot of cDNA was used as a template in a standard PCR reaction, using the primers Rh5-F (5′-ATGATAAGAATAAAAAAAAAATTAATTTTGACCATT-3′) and Rh5-R (5′-TCATTGTGTAAGTGGTTTATTTTTTTTATATGTTTG-3′). Amplified fragments were subcloned into pCR2.1-TOPO, using the TOPO TA Cloning Kit (Invitrogen) and three clones from each strain were sequenced and analysed.

Antibodies

Antibodies were obtained from the following suppliers: anti-rat Cd4d3+4 (OX68) (AbD Serotec), anti-CD59 (AbD Serotec), mouse IgG1 control (Abcam). Anti-BSG monoclonal antibodies used were 8J251 (Lifespan Biosciences), MEM-M6/1 (Abcam) and TRA-1-85 (R&D systems). MEM-M6/6 was provided as an ascitic fluid and was a gift of V. Horejsi; the antibody was purified using a HiTrap protein G column (GE Healthcare) as described31 and exchanged into RPMI.

Surface plasmon resonance

Surface plasmon resonance studies were performed using a Biacore T100 instrument. Briefly, biotinylated bait proteins were captured on a streptavidin-coated sensor chip (Biacore, GE Healthcare). Approximately 150 response units (RU) of the negative control bait (biotinylated rat Cd4d3+4) were immobilised in the flow cell used as a reference and approximate molar equivalents of the query protein immobilised in other flow cells. Purified analyte proteins were separated by gel filtration just before use in SPR experiments to remove small amounts of protein aggregates which are known to influence kinetic binding measurements32. Increasing concentrations of purified proteins were injected at high flow rates (100µlmin−1) to minimise rebinding effects for kinetic studies or at 10µlmin−1 for equilibrium analysis. Although essentially all the bound PfRh5 dissociated during the wash out phase (see Fig. 1c), the surface was ‘regenerated’ with a pulse of 2M NaCl at the end of each cycle. Duplicate injections of the same concentration in each experiment were superimposable, demonstrating no loss of activity after regenerating the surface. Both kinetic and equilibrium binding data were analysed in the manufacturer’s Biacore T100 evaluation software (Biacore). Equilibrium binding measurements were taken once equilibrium had been reached using reference-subtracted sensorgrams. Both the kinetic and equilibrium binding studies involving BSG-S and variants were performed three times using independent protein preparations of both PfRh5 and the BSG proteins, and once for BSG-L and its variants. All experiments were performed at 37°C.

Enzyme-linked immunosorbent assay (ELISA)

Biotinylated ectodomains were immobilized on streptavidin-coated plates (Nunc) for 1h before incubation for 90min with 10μgml−1 primary antibody. The plates were washed in HBS/0.1% Tween-20 (HBST) before incubation with an appropriate secondary antibody conjugated to alkaline phosphatase (Sigma). Plates were washed three times in HBST and once in HBS before adding 100µl p-nitrophenyl phosphate (Sigma 104 alkaline phosphatase substrate) at 1mgml−1. Optical density measurements were taken at 405nm on a Pherastar plus (BMG laboratories). The whole procedure was performed at room temperature.

Change history

Corrected online 21 December 2011
The Competing Financial Interests statement appeared incorrectly in the AOP PDF version. This has been corrected.

References

  1. Maier, A. G. et al. Plasmodium falciparum erythrocyte invasion through glycophorin C and selection for Gerbich negativity in human populations. Nature Med. 9, 8792 (2003)
  2. Mayer, D. C. et al. Glycophorin B is the erythrocyte receptor of Plasmodium falciparum erythrocyte-binding ligand, EBL-1. Proc. Natl Acad. Sci. USA 106, 53485352 (2009)
  3. Sim, B. K. et al. Receptor and ligand domains for invasion of erythrocytes by Plasmodium falciparum. Science 264, 19411944 (1994)
  4. Tham, W. H. et al. Complement receptor 1 is the host erythrocyte receptor for Plasmodium falciparum PfRh4 invasion ligand. Proc. Natl Acad. Sci. USA 107, 1732717332 (2010)
  5. Cowman, A. F. & Crabb, B. S. Invasion of red blood cells by malaria parasites. Cell 124, 755766 (2006)
  6. Baum, J. et al. Reticulocyte-binding protein homologue 5 – an essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum. Int. J. Parasitol. 39, 371380 (2009)
  7. Iyer, J. et al. Invasion of host cells by malaria parasites: a tale of two protein families. Mol. Microbiol. 65, 231249 (2007)
  8. Hayton, K. et al. Erythrocyte binding protein PfRH5 polymorphisms determine species-specific pathways of Plasmodium falciparum invasion. Cell Host Microbe 4, 4051 (2008)
  9. Rodriguez, M. et al. PfRH5: a novel reticulocyte-binding family homolog of Plasmodium falciparum that binds to the erythrocyte, and an investigation of its receptor. PLoS ONE 3, e3300 (2008)
  10. Pasini, E. M. et al. In-depth analysis of the membrane and cytosolic proteome of red blood cells. Blood 108, 791801 (2006)
  11. Bushell, K. M. et al. Large-scale screening for novel low-affinity extracellular protein interactions. Genome Res. 18, 622630 (2008)
  12. Martin, S. et al. Construction of a large extracellular protein interaction network and its resolution by spatiotemporal expression profiling. Mol. Cell. Proteomics 9, 26542665 (2010)
  13. Söllner, C. & Wright, G. J. A cell surface interaction network of neural leucine-rich repeat receptors. Genome Biol. 10, R99 (2009)
  14. Spring, F. A. et al. The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, basigin and neurothelin, an immunoglobulin superfamily molecule that is widely expressed in human cells and tissues. Eur. J. Immunol. 27, 891897 (1997)
  15. Igakura, T. et al. A null mutation in basigin, an immunoglobulin superfamily member, indicates its important roles in peri-implantation development and spermatogenesis. Dev. Biol. 194, 152165 (1998)
  16. Fadool, J. M. & Linser, P. J. 5A11 antigen is a cell recognition molecule which is involved in neuronal-glial interactions in avian neural retina. Dev. Dyn. 196, 252262 (1993)
  17. Wright, G. J. Signal initiation in biological systems: the properties and detection of transient extracellular protein interactions. Mol. Biosyst. 5, 14051412 (2009)
  18. Williams, B. P. et al. Biochemical and genetic analysis of the Oka blood group antigen. Immunogenetics 27, 322329 (1988)
  19. Guo, H. et al. Stimulation of matrix metalloproteinase production by recombinant extracellular matrix metalloproteinase inducer from transfected Chinese hamster ovary cells. J. Biol. Chem. 272, 2427 (1997)
  20. Anstee, D. J. The nature and abundance of human red cell surface glycoproteins. J. Immunogenet. 17, 219225 (1990)
  21. Theron, M., Hesketh, R. L., Subramanian, S. & Rayner, J. C. An adaptable two-color flow cytometric assay to quantitate the invasion of erythrocytes by Plasmodium falciparum parasites. Cytometry A 77A, 10671074 (2010)
  22. Neafsey, D. E. et al. Genome-wide SNP genotyping highlights the role of natural selection in Plasmodium falciparum population divergence. Genome Biol. 9, R171 (2008)
  23. Bei, A. K., Brugnara, C. & Duraisingh, M. T. In vitro genetic analysis of an erythrocyte determinant of malaria infection. J. Infect. Dis. 202, 17221727 (2010)
  24. Durbin, R. M. et al. A map of human genome variation from population-scale sequencing. Nature 467, 10611073 (2010)
  25. Jallow, M. et al. Genome-wide and fine-resolution association analysis of malaria in West Africa. Nature Genet. 41, 657665 (2009)
  26. Teo, Y. Y., Small, K. S. & Kwiatkowski, D. P. Methodological challenges of genome-wide association analysis in Africa. Nature Rev. Genet. 11, 149160 (2010)
  27. Sabeti, P. C. et al. Genome-wide detection and characterization of positive selection in human populations. Nature 449, 913918 (2007)
  28. Schlegel, J. et al. Solution characterization of the extracellular region of CD147 and its interaction with its enzyme ligand cyclophilin A. J. Mol. Biol. 391, 518535 (2009)
  29. Yu, X. L. et al. Crystal structure of HAb18G/CD147: implications for immunoglobulin superfamily homophilic adhesion. J. Biol. Chem. 283, 1805618065 (2008)
  30. Snounou, G. & Beck, H. P. The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment. Parasitol. Today 14, 462467 (1998)
  31. Crosnier, C., Staudt, N. & Wright, G. J. A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biol. 8, 76 (2010)
  32. van der Merwe, P. A. & Barclay, A. N. Analysis of cell-adhesion molecule interactions using surface plasmon resonance. Curr. Opin. Immunol. 8, 257261 (1996)

Download references

Acknowledgements

We are grateful to the Oka− blood donors. We thank V. Horejsi for monoclonal antibodies and D. Ahr for technical assistance. This work was supported by the Wellcome Trust grant numbers 077108 (G.J.W.) and 089084 (J.C.R.) and National Institutes of Health R01AI057919 (M.T.D.). A.K.B. is supported by a Center for Disease Control grant R36 CK000119-01 and an Epidemiology of Infectious Disease and Biodefense Training Grant 2T32 AI007535-12.

Author information

  1. These authors contributed equally to this work.

    • Cécile Crosnier,
    • Leyla Y. Bustamante &
    • S. Josefin Bartholdson

Affiliations

  1. Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK

    • Cécile Crosnier,
    • S. Josefin Bartholdson &
    • Gavin J. Wright
  2. Malaria Programme, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK

    • Leyla Y. Bustamante,
    • Michel Theron,
    • Dominic P. Kwiatkowski,
    • Julian C. Rayner &
    • Gavin J. Wright
  3. Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA

    • Amy K. Bei &
    • Manoj T. Duraisingh
  4. Tokyo Red Cross Blood Center, Tokyo 135-8639, Japan

    • Makoto Uchikawa
  5. Laboratory of Bacteriology and Virology, Le Dantec Hospital and Laboratory of Parasitology, Cheikh Anta Diop University, BP: 7325, Dakar, Senegal

    • Souleymane Mboup &
    • Omar Ndir
  6. Wellcome Trust Centre for Human Genetics, Oxford OX3 7BN, UK

    • Dominic P. Kwiatkowski

Contributions

C.C. compiled the erythrocyte protein library and identified the PfRh5–BSG interaction. L.Y.B. led the P. falciparum functional validation, with support from M.T. S.J.B. performed the biochemical and biophysical characterization of the interaction. A.K.B. performed the lentiviral knockdown and parasite invasion experiments under the direction of M.T.D. M.U. provided the Oka− blood samples and matching controls. O.N. and S.M. supervised the collection and culturing of field strains. D.P.K. performed genetic analysis on the BSG and PfRh5 loci. G.J.W. and J.C.R. conceived and supervised the project, and wrote the manuscript.

Competing financial interests

C.C., L.Y.B., S.J.B., J.C.R. and G.J.W. are named on a patent application relating to this work.

Corresponding authors

Correspondence to:

Author details

Supplementary information

PDF files

  1. Supplementary Information (1.5M)

    The file contains Supplementary Figures 1-9 with legends and Supplementary Tables 1-4.

Additional data