Abstract
Neural-Wiskott Aldrich Syndrome Protein (N-WASP) is expressed ubiquitously, regulates actin polymerization and is essential during mouse development. We have previously shown that N-WASP is critical for cell-ECM adhesion in fibroblasts. To characterize the role of N-WASP in fibroblast for skin development, we generated a conditional knockout mouse model in which fibroblast N-WASP was ablated using the Cre recombinase driven by Fibroblast Specific Protein promoter (Fsp-Cre). N-WASPFKO (N-WASPfl/fl; Fsp-cre) were born following Mendelian genetics, survived without any visible abnormalities for more than 1 year and were sexually reproductive, suggesting that expression of N-WASP in fibroblast is not critical for survival under laboratory conditions. Histological sections of N-WASPFKO mice skin (13 weeks old) showed thicker epidermis with higher percentage of cells staining for proliferation marker (PCNA), suggesting that N-WASP deficient fibroblasts promote keratinocyte proliferation. N-WASPFKO mice skin had elevated collagen content, elevated expression of FGF7 (keratinocyte growth factor) and TGFβ signaling proteins. Wound healing was faster in N-WASPFKO mice compared to control mice and N-WASP deficient fibroblasts were found to have enhanced collagen gel contraction properties. These results suggest that N-WASP deficiency in fibroblasts improves wound healing by growth factor-mediated enhancement of keratinocyte proliferation and increased wound contraction in mice.
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Introduction
The skin is the largest organ in the body and is composed of two primary layers; the epidermis and the dermis which are separated by a basement membrane1. The epidermis is composed mainly of keratinocytes whereas the dermis is composed mainly of connective tissue produced by dermal fibroblasts. As a protective organ, the skin requires mechanical toughness and the ability to repair itself after wounding. Wound healing can be broken down to 3 stages, the inflammatory phase, proliferation phase and the maturation phase. During the inflammatory phase, the activated platelets play an important role in the formation of a clot to stop bleeding and maintain tissue homeostasis. Both the keratinocyte and fibroblasts play a critical role during the proliferation phase. The keratinocytes proliferate and migrate to re-epithelize the epidermis layer and cover the wound while underlying fibroblasts migrate and is activated to synthesize extracellular matrix proteins for connective tissue2,3. Upon tissue injury, fibroblasts differentiate into myofibroblasts and promote wound healing by decreasing the size of the wound in mice. Myofibroblasts express highly contractile protein α-smooth muscle actin (α-SMA) which provides adhesive and biochemical force required for wound closure4,5. Several studies have shown the importance of fibroblasts in wound healing. Rac1 knockout in dermal fibroblast led to delay in wound healing, reduced myofibroblast activation and lowered collagen production6. Fibroblast specific deletion of TGFβ receptor diminished the wound contraction, dermal regeneration and re-epithelialization7. Similarly, reduced TGFβ signaling was identified in fibroblast specific integrin β1 deficient mice8. All these studies suggest that the dermal fibroblast is critical for in-vivo tissue repair.
Keratinocytes and fibroblasts communicate through the secretion of growth factors and cytokine9. During wound healing, fibroblasts within the wound bed secrete keratinocyte growth factor (KGF) that triggers keratinocytes to proliferate, facilitating re-epithelization10. Moreover, exogenous KGF-1 expression into the excisional wounds of genetic diabetic mouse models, improved their wound healing rate and epithelialization11. 12-O-tetradecanoylphorbol-13-acetate (TPA) induced epithelial cells proliferation and local inflammation was found to be reduced in the absence of proliferating fibroblast12. In short, fibroblasts play an important role in the secretion of growth factors required for keratinocyte proliferation during the skin wound healing process.
Cell migration, adhesion, morphology, growth factor secretion, proliferation and other critical cellular process are regulated by the actin cytoskeleton13. N-WASP (Neural-Wiskott Aldrich Syndrome protein) which is expressed ubiquitously and has been shown to be a critical regulator of actin cytoskeleton remodeling via Arp2/3 complex14. N-WASP deficiency in fibroblast leads to reduced cell-ECM adhesion and increased cell motility15. N-WASP regulates invadopodia formation16 in Src transformed fibroblasts17,18 and affects clathrin dependent19 or independent endocytosis20. Moreover, N-WASP has been found to play critical role in RNA polymerase-II dependent transcription regulation, RNA processing and DNA repair via interaction with PSF-NonO complex21. N-WASP expression has been found to be lower in breast cancer cells22, whereas it highly expressed in hepatocellular carcinoma23.
N-WASP deficient mice had abnormalities in neural tube formation, heart and mesoderm differentiation and die at embryonic stage E11.524,25. Conditional knockout of N-WASP expression in mouse brain leads to severe hydrocephalus condition and premature post-natal death26. N-WASP is also critical for T-cells development27. Conditional ablation of N-WASP expression in keratinocytes leads to ulceration, alopecia and marked epidermal hyperproliferation. This disrupted hair follicle cycling was due to increased TGFβ signaling28 or due to decreased Wnt-dependent transcription29.
In this study we have characterized the in-vivo role of N-WASP in dermal fibroblasts using Fsp-Cre (fibroblast specific protein) mice to selectively ablate the expression of N-WASP in fibroblasts. N-WASPfl/fl; Fsp-cre (N-WASPFKO) mice were born following Mendelian genetics suggesting that deficiency of N-WASP in fibroblast does not lead to lethality. Both the male and female N-WASPFKO mice were sexually active and did not show any physical abnormalities. Hematoxylin and eosin staining of skin sections of the N-WASPFKO mice showed thicker epidermis suggesting that N-WASP deficiency in dermal fibroblasts leads to epidermal hyperproliferation in aged mice. Moreover, the dermal wound closure was found to be faster in N-WASPFKO mice compared with the controls. In-vitro collagen gel contraction assay showed that N-WASP deficient fibroblast caused faster collagen gel contraction in the presence of TGFβ. Expression of growth factor KGF/FGF7 was elevated in N-WASPFKO mice. In addition the expression of TGFβ signaling pathway proteins (Smad2, Smad3, Tak1 and TGFβ-RII) was increased in N-WASPFKO mice compared to control. Thus, our results suggest that the ablation of N-WASP expression in dermal fibroblasts enhanced wound healing, in part, by promoting proliferation of keratinocytes and enhanced wound contraction. Our data provide new insights into the functions of N-WASP in dermal fibroblasts regulating epidermal cell proliferation.
Results
Loss of N-WASP expression in dermal fibroblasts caused epidermal hyper proliferation in aged mice
In this study, we have generated dermal fibroblast specific N-WASP knockout mice by crossing N-WASPfl/flmice with Fsp-cre mice (Fig. S1A). The heterozygous, N-WASPfl/WT; Fsp-cre mice from the first generation mice were back crossed to get N-WASPfl/fl; Fsp-cre (N-WASPFKO) mice near to the Mendelian 25% ratio (Fig. S1B). Excision of exon 3 and 4 of (floxed) N-WASP gene was detected in N-WASPFKO mice (Fig. S1C) in tail genomic PCR. Primary dermal fibroblasts isolated from N-WASPFKO mice showed efficient knockdown of N-WASP expression at 13 weeks after birth (Fig. 1A,B). N-WASPFKO mice exhibited a normal life span and no obvious defects were observed in body weight (Fig. S1D), external morphology, reproductive vigor and behavior compared to control littermates (Data not shown). N-WASP expression in keratinocytes has been shown to play critical role in hair follicle cycling via the anti-proliferative TGFβ pathway28. Histological analysis of skin layers of N-WASPFKO mice did not show any obvious differences in hair follicle pattern or numbers between weeks 4 to 6 after birth (Fig. S2A). Histological analysis of mice skin taken after 13 weeks or nearly 1 year after birth showed a significant increase in epidermal layer thickness in N-WASPFKO mice compared to either heterozygous (N-WASPfl/WT; Fsp-cre) or control (N-WASPfl/fl) mice (Fig. 1C,D S2B). There was no significant difference in the dermal thickness of mice skin (Fig. 1E). In addition, histological analysis of the ear also showed an enhanced thickening in the N-WASPFKO mice, compared to the control littermates (Data not shown). The thickening of the epidermal layer of N-WASPFKO mice was further characterized by visualizing the nucleus and E-cadherin structures which showed the presence of more than two layers of cells in the epidermis. There was no obvious alteration in the epidermal cell-cell junction, distribution of E-cadherin between control and N-WASPFKO mice (Fig. 2A). In order to verify that the thickening in epidermal layer in N-WASPFKO mice skin is due to a proliferative defect within the epidermis, we performed the immuno-staining of paraffin embedded skin sections for the proliferation marker PCNA. Basal level of PCNA positive cells count revealed that N-WASPFKO mice has significantly higher (40 ± 5, 13 weeks; 36 ± 10, 1-year-old) number of PCNA positive keratinocytes compared with control mice (12 ± 3, 13 weeks; 8 ± 1, 1-year-old) (Fig. 2B,C). Furthermore, Western blot analysis of whole skin lysate showed higher expression of PCNA in N-WASPFKO mice (Fig. 2D). Thus, suggesting that N-WASPFKO mice have defects in regulating the proliferation of keratinocytes resulting in epidermal hyperproliferation.
N-WASPFKO mice do not have defect in skin barrier function
In order to analyze the skin barrier function we isolated a section of skin from control and N-WASPFKO mice and incubated with Lucifer yellow on the epidermis. The skin section was subsequently analyzed by histological staining to visualize the presence of Lucifer yellow using fluorescent microscopy. In both control and N-WASPFKO, fluorescent dye was restricted to the outer layer of epidermis suggesting that there was no obvious defect in the skin barrier function of the epidermis in N-WASPFKO mice (Fig. 3A). The differentiation of keratinocytes in N-WASPFKO and control mice was analyzed by immunofluorescence staining for early and terminal differentiation markers (keratin 10, involucrin, transglutaminase). There were no significant differences in the staining pattern of the differentiation markers between control and N-WASPFKO mice, however there was a thicker layer of cells staining for keratin 10, involucrin and transglutaminase. This suggests that N-WASP deletion in dermal fibroblast cells do not alter epidermal cells differentiation (Fig. 3B).
TPA induced hyperproliferation response
In order to investigate the cutaneous response under the stress condition, we treated the skin of control and N-WASPFKO mice topically with the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA). Expectedly, a single treatment of 6.5 nM/50 μl TPA for 24 hours on dorsal skin resulted in significant thickening of epidermis of both control and N-WASPFKO mice compared with vehicle-treated mice (Fig. 4A,B). Histological analyses of TPA-treated skin sections revealed an elevated hyperproliferative effect in N-WASPFKO mice skin, compared to wildtype littermates. (Fig. 4A), with a concomitant increase in the number of PCNA-positive proliferating keratinocytes in N-WASPFKO mice skin (Fig. 4C). There was no alteration in the epidermal cell-cell junctions identified in stressed induced (TPA-treated) N-WASPFKO mice skin as localization and pattern of E-cadherin was comparable to TPA treated control mice skin (Fig. 4D). Thus, TPA treatment led to enhanced epidermal cell hyperproliferation in N-WASPFKO mice compared to control mice.
Loss of N-WASP expression in dermal fibroblast results in faster wound closure
Dermal fibroblasts play a significant role in wound healing and tissue repair30. In order to determine the effect of N-WASP deficiency in skin fibroblast on tissue repair, we carried out a wound healing assay on 13–15 weeks old control and N-WASPFKO mice. Mice were subjected to full-thickness excisional wounds and the wounded area of mice were photographed and quantified at indicated time points (Fig. 5). N-WASPFKO mice showed a significant faster wound closure at post-wounding days 2, 5 and 7 compared with control mice (Fig. 5A,B). Moreover, significant reduced wound diameter (shown by arrows) was observed at day 7 in N-WASPFKO mice (Fig. 5C,D). In order to characterize the accelerated wound healing phenotype in N-WASPFKO mice, PCNA staining was performed on skin sections on the 7th day after wounding. We found significantly more number of PCNA positive cells in both dermal and epidermal region in N-WASPFKO mice skin compared to control mice (Fig. 5E). Thus, suggesting that enhanced proliferation rate of dermal/epidermal skin cells after wounding promote faster wound closure in N-WASPFKO mice.
Wound healing involves re-epithelialization as well as wound contraction31. Activated keratinocyte proliferate, migrate and subsequently differentiate to form new epithelium while activated fibroblast promote wound healing by wound contraction2,3,4,5. In order to characterize the role of N-WASP in wound contraction, we carried out collagen gel contraction assay. We embedded equal number (2 × 105 cells) of WT or N-WASPKO mouse embryonic fibroblasts (MEFs)25 in Type I collagen matrix. The collagen gel was detached from the side and stimulated with TGFβ1 to measure gel contraction in free floating collagen gel (Fig. 5F). The gel surface area was measured at 24 hr interval for 96 hours. Collagen gel in the absence of fibroblasts or with cells but without TGFβ1 treatment did not contract (note: a slight collagen gel contraction was observed with N-WASPKO MEF cells even without TGFβ1 (Fig. S3C). In the presence of TGFβ1, collagen gel embedded with N-WASPKO fibroblasts contracted to larger extent compared to embedded with N-WASPWT fibroblasts. (Fig. 5G). Thus, the faster wound healing observed in the N-WASPFKO mice could be due to enhanced wound contraction by the N-WASP deficient fibroblasts.
N-WASPFKO mice have increased fibroblasts and collagen
Wound healing is a multistep process comprising of, the inflammatory phase, proliferation phase and the maturation phase32. In order to determine whether differences in the inflammatory responses could contribute to increased wound healing in N-WASPFKO mice, staining for lymphocytes (CD3/CD4) and neutrophils (Ly-6G antigen) was performed in the granulation tissue. Expression of N-WASP did not significantly alter the ability of wounded tissue to recruit neutrophils, but moderately enhanced the recruitment of lymphocytes (Fig. S4A). Additionally, under basal conditions, the skin of control and N-WASPFKO mice had a very low content of immune cells (data not shown). A cardinal feature of poorly healing wounds is a persistent inflammatory response at the wound site33. Despite a slight increase in immune cell infiltration into the wounds of N-WASPFKO mice, the wound closed faster, suggesting that inflammation is unlikely a major contributor to the faster wound healing in N-WASPFKO mouse.
Fibroblasts have been shown to accumulate at the wounded tissue and promote re-epithelialization by producing ECM (collagen) and growth factors34. Although Vimentin is expressed by endothelial cells, it can still be used to identify fibroblast35,36,37. Immunohistochemistry analysis revealed that N-WASPFKO mice wound had a significant increase in fibroblast number compared to control mice (Fig. 6A). In addition, Western blot analysis also showed increased vimentin expression in N-WASPFKO mice skin (Fig. 6B). Moreover, a moderate increase in vimentin staining was observed in normal N-WASPFKO mice skin (without wounded) compared to control mice skin (Fig. S3A). Dermal fibroblast cells can also differentiate into myofibroblasts and can promote wound healing. Therefore, we analyzed the effect of N-WASP deletion on myofibroblast conversion. N-WASPFKO mice skin showed significant enhanced expression of α-SMA compared to control mice indicating that N-WASP deletion in dermal fibroblast affect myofibroblast differentiation (Fig. 6C). We further evaluated the amount of matrix deposition (collagen content) by Masson’s trichrome staining. A moderate increase in collagen content in 7 day wound section was observed in N-WASPFKO mice compared to control mice (Fig. 6D). Intense collagen staining was also observed in N-WASPFKO mice skin (non-wounded) (Fig. S3B). This could be due to the increased number of dermal fibroblast in N-WASPFKO mice skin.
TGFβ signaling is increased in N-WASPFKO mice skin
The higher collagen content in the granulation tissue of N-WASPFKO mice suggested the possibility of increased in growth factor secretion. Moreover, crosstalk between fibroblast and keratinocyte occurs through secreted growth factors are essential during wound healing. Members of fibroblast growth factor family (FGFs) have a broad mitogenic spectrum except FGF7/KGF, which is a specific mitogen for epithelial cells38. Analysis of FGF7 expression revealed increase in FGF7 staining in N-WASPFKO mice skin (Fig. 6E). Moreover, moderate increase in pro-inflammatory cytokine IL-1α was also observed in N-WASPFKO mice skin (Fig. S4B). N-WASP expression in keratinocytes has been shown to regulate TGFβ signaling28. Based on this information, we analyzed TGFβ signaling using skin lysate from control and N-WASPFKO mice. N-WASPFKO mice expressed higher levels of TGFβ-RII, Samd2/3, TAK1, and their respective activated forms (p-Samd2/3, pTGFβ-RII) (Fig. 6F, S4C). Our results suggest that deletion of N-WASP expression in dermal fibroblast leads to increase in fibroblasts number, leading to increase in collagen content and secretion of growth factors/cytokines/enhanced TGFβ signaling which results in epidermal hyperproliferation and faster wound healing in N-WASPFKO mice.
Discussion
N-WASP, a member of the WASP family of proteins, regulates the actin polymerization by regulating the activity of the Arp2/3 complex14. N-WASP is expressed ubiquitously and N-WASP knockout mice were found to be embryonically lethal suggesting that N-WASP is an essential gene in during the development of mice24,25. The N-WASP knockout embryos were found to survive past gastrulation stage and initiate organogenesis but die before embryonic day 12 with neural tube and cardiac defects25. Thus a number of studies have used the loxP-Cre system to generate conditional N-WASP knockout mice to study the role of N-WASP in various tissues. Conditional knockout of N-WASP in muscles using Myf5-cre and MyoD-cre resulted in post-natal death39. Similarly, conditional knockout of N-WASP in brain using Nestin-cre also resulted in post-natal death probably due to hydrocephalus26. Two research groups have generated conditional knockout mice with expression of N-WASP ablated in keratinocytes using K5-cre28,29, however the two groups reported contrasting roles for N-WASP in keratinocytes. Leferver et al. showed that lack N-WASP expression in keratinocytes caused reduced growth of the keratinocytes while Lyubimova et al. showed that N-WASP deficiency leads to epidermal hyper proliferation28,29.
We have previously found that N-WASP in fibroblasts plays a critical role in cell-ECM adhesion15. In order to characterize the role of N-WASP in fibroblasts in the development and maintenance of skin, we generated mice deficient for N-WASP expression in fibroblasts using Fsp-cre as the driver. Mice with the genotype of N-WASPfl/fl; Fsp-cre, N-WASPFKO were born following Mendelian genetics, and did not show any abnormalities up to 1 year of growth in the laboratory. There were neither physical abnormalities nor reduced sexual vigor observed in N-WASPFKO mice compared with controls (Data not shown).
Histological sections of the mice skin showed that the N-WASPFKO mice had a thicker epidermis compared with controls and immunostaining for PCNA suggested that the keratinocytes in N-WASPFKO mice were undergoing hyperproliferation (Fig. 2). The fibroblasts and keratinocytes in the skin have a complex interaction in order to maintain homeostasis of the skin and are very critical during wound healing31,40. Thus we characterized the wound healing characteristics of the N-WASPFKO mice and found that N-WASPFKO mice healed the wound faster than that of control mice, the difference was significant at all the time points analyzed (Fig. 5A–D). Conditional knock out N-WASP in keratinocytes led to hyperproliferation however there was no difference in the wound healing characteristics of the knockout mice compared to the control29. Thus the hyperproliferation of keratinocytes seen in N-WASPFKO is probably not the sole reason for faster wound healing in N-WASPFKO. Upon injury the fibroblasts promotes wound healing by several mechanisms31,41, the fibroblasts proliferate, synthesis collagen and differentiate to myofibroblasts which helps in wound healing by contracting the wound31,42. Thus we analyzed the collagen gel contraction properties of WT and N-WASP deficient fibroblasts by TGFβ treatment of cells embedded in collagen matrix. The N-WASP deficient fibroblasts induced a larger gel contraction compared with WT fibroblasts (Fig. 5G). Notably, our finding was in contrast to a previous in vitro study that suggested a role of N-WASP in integrating FAK and Arp2/3 signaling to mediate formation of α-SMA-containing cytoplasmic filaments during myofibroblast differentiation and maturation43. In that study, fibroblasts with decreased N-WASP expression (N-WASP knockdown) showed impaired ability to contract collagen gels in response to TGF-β143. The reason for this discrepancy could be due to the difference between the type of cells used. We used knockout cells while Cai et al., used knockdown cells. A number of studies have shown that knockout cells may have different phenotypes to knockdown cells; caveolin-1 knockout cells had higher proliferation than control WT cells while caveolin-1 knockdown (80% reduction) did not exhibit increased proliferation44. Expression of N-WASP was found to be higher in chronic wound compared to acute wound45. HaCaT cells (keratinocytes) treated with N-WASP inhibitors (Wiskostatin or 178-1) had increased cell migration. Similarly, knocking down N-WASP expression also caused increased cell migration45. Both the chemical inhibitors were found to enhance wound healing in mice, suggesting that inhibition of N-WASP promotes wound healing45. The inhibitors are likely to inhibit N-WASP in all cell types thus it is not possible to identify the cell in which N-WASP activity has to be modulated to promote wound healing. Our studies are consistent with the finding that N-WASP inhibition promotes wound healing.
Various growth factors such as fibroblast growth factors (FGFs), platelet derived growth factors (PDGFs) and cytokines play critical roles during wound healing38. PDGFs have been shown to enhance the fibroblast cells proliferation, extracellular matrix production and stimulates fibroblast contraction in collagen matrices46. Moreover, impaired wound healing in aged mice is associated with delay in appearance of PDGF receptors47. In our study, N-WASPFKO mice skin had increased number of fibroblast compared to control mice as determined by elevated expression of vimentin and increased number vimentin positive cells (Fig. 6A,B). The increased number of fibroblast probably led to enhanced deposition of collagen in N-WASPFKO mice wounded skin tissue (Fig. 6D). A moderate but not significant increase in PDGFR was also observed in N-WASPFKO mice skin (Data not shown). Among FGF family members, FGF7 has been shown to specifically target keratinocytes. FGF7 is secreted by dermal fibroblast and its expression is elevated by injury to skin38. Previous reports suggest that FGF7 induces epidermal cell hyperproliferation and migration without affecting their final differentiation or barrier function, and thus promoted faster healing of wounds48. In addition, epidermal atrophy with dermal thickening and delay in re-epithelialization of wounds has been reported in transgenic mice expressing dominant negative FGF7 receptor49. Consistent with previous studies, expression of FGF7 was found to be elevated in N-WASPFKO mice skin compared to control mice (Fig. 6E) which probably caused epidermal hyperproliferation (without altering epidermal cell differentiation and barrier function) (Fig. 3) and faster wound healing. Moreover, expression of inflammatory cytokines IL-1α was found to be upregulated in N-WASPFKO mice skin (Fig. S4B). IL-1α is secreted by various kinds of cells such as neutrophils, macrophages and fibroblasts. It is very likely that IL-1α stimulates keratinocytes and fibroblast proliferation and regulates wound healing as tropical application of IL-1α accelerated epidermal wound healing50. The relationship between IL-1α and FGF7 underscores an important epithelial-mesenchymal communication for skin homeostasis. Keratinocytes are known to synthesize IL-1α in response to injury and IL-1α has been shown to stimulate fibroblast and keratinocyte growth, collagen synthesis by fibroblasts, and chemotaxis of keratinocytes51,52. In a double paracrine manner, keratinocyte IL-1α stimulates the expression FGF7 in fibroblasts53,54. The fibroblast FGF7 enhances epidermal proliferation. The deletion of N-WASP in the fibroblast perturbed this epithelial-mesenchymal communication, which manifested as epidermal hyperproliferation and increased fibroblast number.
Fibroblasts produce many components of ECM and are also a relevant source of TGFβ55. Our results suggest that N-WASP modulates TGFβ signaling in fibroblasts as we found enhanced expression of TGFβ signaling proteins in N-WASPFKO mice skin (Fig. 6F, S4C) and N-WASPKO MEFs caused a slight but significant in-vitro collagen gel contraction even in the absence of exogenous TGFβ (Fig. S3C). Both WT MEFs and N-WASPKO MEF caused in-vitro collagen gel contraction in the presence of exogenous application of TGFβ however the N-WASPKO MEF caused significantly higher gel contraction (Fig. 5G). TGFβ signal through binding to two TGF-β receptors (TGFβ-RI and TGFβ-RII). TGFβ-RII is constitutively phosphorylated and on binding of the ligand, phosphorylates TGFβ-RI. Phosphorylation of the receptor complex activates the SMAD intracellular signaling pathway through the receptor Smads (Smad-2 and Smad-3) and co-Smad 4. The receptor SMADs and Smad-4 cross over the nuclear membrane where they regulate a number of genes56. In N-WASPFKO mice skin, expression of Smad-2 and Smad-3 and respective phospho-Smad2 and phospho-Smad-3 were elevated (note: we do not observe any difference in ratio of p-Samd3/Samd3 or p-TGFβ-RII/TGFβ-RII between control and N-WASPFKO mice skin, thus enhanced expression of these TGFβ signaling associated factors is probably causing the significant differences in the wound healing between control and N-WASPFKO mice) (Fig. S4C). This enhanced expression of Smad-2 might have up-regulated the expression of receptor TGFβ-RII via positive feedback loop and thus representing the increased TGFβ signaling in N-WASPFKO mice skin56. TGFβ is a mediator of tissue repair, wound healing and has been widely implicated in progressive tissue fibrosis57. TGFβ drives fibroblast-myofibroblast differentiation. Myofibroblasts express α-smooth muscle actin (α-SMA) and are present in granulation tissue where they are responsible for wound contraction. TGFβ has been shown to upregulate the expression of α-SMA both in vitro and in vivo58,59. Conceivably, the enhanced TGFβ signaling stimulates fibroblast proliferation and also activates normal fibroblasts to become myofibroblasts. A recent study suggests that reconstitution of vimentin expression in vimentin−/− fibroblasts restored their proliferation and TGFβ production, thus is critical for wound healing60. This study further potentiates our observation; enhanced vimentin expression/enhanced fibroblasts proliferation/enhanced TGFβ signaling. The enhanced TGFβ signaling associated factors in N-WASPFKO mice skin probably promotes fibroblasts to myofibroblasts differentiation and faster wound healing. Taken together; our observations suggest that ablation of N-WASP expression in dermal fibroblasts, increase fibroblast cell number, which is probably due to the activation of fibroblasts by autocrine/paracrine signaling. This activation of fibroblasts in N-WASPFKO mice, enhanced expression/secretion of growth factors/cytokine (FGF7/IL-1α/TGFβ signaling) or collagen deposition, resulted in epidermal hyperproliferation and accelerated wound healing in aged mice (Fig. 6G).
Materials and Methods
Animals
N-WASPfl/fl mice were generated as described previously26. In order to generate mouse skin fibroblast cells specific N-WASP expression deletion, N-WASPfl/fl mice were crossed with Fsp-cre (fibroblast specific protein) mice followed by back cross. Genotyping of mice were performed as described previously26. In brief, small piece of tail tips (2–5 mm) were digested in tail digestion buffer containing 50 mMKCl, 10 mMTris–HCl (pH 9.0), 0.1% Triton X-100, and 0.4 mg/mL Proteinase K (Sigma-Aldrich; P2308). Tail PCR was performed using KAPA HiFi PCR Kit (KK2101). The primers used to detect flox/flox were (5′ to 3′ direction); AGCTCAGAGAAGGTGTATTGG (forward) and AGGA CTTACATCTCCAGCAAAGG (reverse). Fsp-cre primers used were: GCGGTC TGGCAGTAAAAACTATC (forward), GTGAAACAGCATTGCTGTCACTT (reverse); internal control primers: CTAGGCCACAGAATTGAAAGATC-3′ (forward), GTAGGT GGAAATTCTAGCATCAT (reverse). In order to detect the deletion of N-WASP at genomic level, another reserve primer (WASL-Flr) has been used together with flox/flox 5′ primer; GGACAGGGTCTATCTCTGAATTCCT. N-WASPfl/fl;Fsp-cre (N-WASPFKO) and control (N-WASPfl/fl) mice were maintained on standard chow diet with unlimited water at constant temperature of 20 °C and 12/12 hours of artificial dark/light cycle. All the experiments were performed according to approved protocols of Institutional Animal Care and Use Committee. All the animal experiments performed were approved by Institutional Animal Care and Use Committee (IACUC) of Nanyang Technological University (NTU), (ARF-SBS/NIE-A0250 and ARF-SBS/NIE-A0236).
Histology
Mice were anaesthetized and sacrificed via cervical dislocation. Dorsal skin of mice was flattened on parafilm and fixed in 4% paraformaldehyde overnight in 4 °C. Fixed dorsal skin tissue was then washed with 1X PBS and dehydrated using a different percentage of absolute ethanol solution (70, 80, 90 and 100%) for 1 hour each followed by dehydration in 50% xylene/ethanol mixture and 100% xylene solution. Dehydrated samples were then submerged in paraffin wax at 60 °C overnight and subsequently embedded in paraffin molds. Paraffin embedded tissue was sectioned to 5 μm size on superfrost slides (Fisher). For staining, slides were placed in 60 °C to dissolve the paraffin and subsequently rehydrated and stained with haematoxylin and eosin. Sections were stained with respective primary antibodies; anti-E-cadherin (BD Bioscience; 610181), keratin 10 (sc-23877), involucrin (sc-28558), transglutaminase (sc-25786), KGF7 (sc-1365) and vimentin (550513, BD Biosciences). Fluorescence labeled secondary antibodies (Alexa-488 or 594; Thermo Fisher Scientific) were used at 1:100 dilution. For analysis of proliferating cells, PCNA antibody (sc-56; PC10) was used to stained paraffin embedded dorsal skin sections. Quantification of PCNA positive cells was performed using ImageJ software61. PCNA positive cells were counted as number of PCNA positive cells per microscopic field. Images were taken using the 40X objective and minimum 6 microscopic fields were counted for each sample for individual experiment and experiment was repeated thrice. Fluorescent images were captured using Olympus microscope fitted with CoolSNAPHQ2 camera.
Wound healing assay
13–15 weeks old control (n = 16) and N-WASPFKO male mice (n = 16) were anesthetized using Ketamine/Xylazine (90 μg/10 μg). Mice were shaved at dorsal side and cleaned with alcohol. Two full thickness circular, (average size of 10 mm in diameter) wounds were made per mouse (separated by at least 1 cm of uninjured skin) using forceps and scissor at dorsal side without injuring the underlying muscle as described62,63. The wounds were covered with occlusive dressing (Tegaderm; 3MTM) in order to prevent any infection. The wounds were photographed with scale using digital camera at 0, 2, 5 and 7th day post injury. The wounded area was determined using imageJ software and wound closure rate was determined as percentage of wound area to the initial wound size. Skin biopsies for histology were performed from each wound at day 0 and 7 after surgery. To visualize collagen, skin sections were stained with masson’s trichrome (Cat No: 25088A-F, Polysciences Inc).
TPA induced hyperproliferation
13–15 weeks old control and N-WASPFKO mice were shaved 24 hours before TPA application (6.5 nM/50 μl in solvent (acetone)) on the dorsal skin. Mice were sacrificed after 24 hours of TPA treatment; skin was isolated, fixed in 4% paraformaldehyde/PBS at 4 °C overnight and embedded in paraffin for histological sections. Sections were stained with haematoxylin and eosin, PCNA and E-cadherin antibodies.
Lucifer yellow penetration assay
For Lucifer yellow penetration assay, 1 mM of Lucifer yellow was poured on to the 1 cm2 piece of skin tissue (dorsal skin, epidermal side) with precautions taken as dye was not allowed to go through the dermal side of the skin. After incubation for 1 hour at 37 °C, the skin was washed excessively with PBS and skin was cryo-sectioned. Cryo-sections were stained with DAPI and analyzed with fluorescence microscope.
Collagen gel contraction assay
Experiment was performed as described previously with some modifications64. N-WASPWT and N-WASPKO mouse embryonic fibroblast cells were maintained in DMEM/10% FBS media. N-WASPWT and N-WASPKO MEFs were kind gift from Scott B Snapper lab25. Mouse embryonic fibroblast cells were trypsinised, counted and 200,000 cells were added into 0.5 mg/ml of Corning Rat Tail Collagen I solution (Corning, USA). 1 μM NaOH was immediately added to neutralize the mixture. The entire mixture was transferred to a 24 well plate immediately. Gel was allowed to set in the 37 °C CO2 incubator for three hours. After the gel has solidified, complete DMEM was added. Solidified Collagen Gel was detached from the side of the well. Pre-diluted TGFβ1 was added to achieve a final concentration of 4 ng/ml. The complete media was renewed every 24 hours using complete DMEM containing TGFβ1. Image was taken and quantified using ImageJ every 24 hours starting with the 0 hour image. Images were taken by placing the 24-wells plate, containing the gel, above a light box with a ruler alongside the gel as a standard to calibrate the pixel size.
Statistical analysis
For statistical significance analysis, unpaired student’s t-test was performed and p value < 0.05 was considered as significance. Values in bar charts are the mean ± S.D from three independent experiments.
Additional Information
How to cite this article: Jain, N. et al. Conditional knockout of N-WASP in mouse fibroblast caused keratinocyte hyper proliferation and enhanced wound closure. Sci. Rep. 6, 38109; doi: 10.1038/srep38109 (2016).
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Acknowledgements
This work was supported by the following grants to TT: Academic Research Fund Tier 1 (MOE), RG46/13 and Academic Research Fund Tier 2 (MOE 2013-T2-2-031).
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N.J., P.K., K.W.T., and H.B.T.: Maintained the mice and carried out experiments with mice as well as histological sectioning/staining of sections. M.K.S. and J.S.K.C. helped in immunostaining and image capture. N.J.: Drafting of the manuscript. T.T. and N.S.T.: Designed the study, analyzed and interpreted the results and wrote the manuscript.
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Jain, N., Kalailingam, P., Tan, K. et al. Conditional knockout of N-WASP in mouse fibroblast caused keratinocyte hyper proliferation and enhanced wound closure. Sci Rep 6, 38109 (2016). https://doi.org/10.1038/srep38109
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DOI: https://doi.org/10.1038/srep38109
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