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Characterizing Focused-Ultrasound Mediated Drug Delivery to the Heterogeneous Primate Brain In Vivo with Acoustic Monitoring

Scientific Reports volume 6, Article number: 37094 (2016) | Download Citation

Abstract

Focused ultrasound with microbubbles has been used to noninvasively and selectively deliver pharmacological agents across the blood-brain barrier (BBB) for treating brain diseases. Acoustic cavitation monitoring could serve as an on-line tool to assess and control the treatment. While it demonstrated a strong correlation in small animals, its translation to primates remains in question due to the anatomically different and highly heterogeneous brain structures with gray and white matteras well as dense vasculature. In addition, the drug delivery efficiency and the BBB opening volume have never been shown to be predictable through cavitation monitoring in primates. This study aimed at determining how cavitation activity is correlated with the amount and concentration of gadolinium delivered through the BBB and its associated delivery efficiency as well as the BBB opening volume in non-human primates. Another important finding entails the effect of heterogeneous brain anatomy and vasculature of a primate brain, i.e., presence of large cerebral vessels, gray and white matter that will also affect the cavitation activity associated with variation of BBB opening in different tissue types, which is not typically observed in small animals. Both these new findings are critical in the primate brain and provide essential information for clinical applications.

Introduction

Blood-brain barrier (BBB) is a highly selective barrier in the cerebral endothelium that prevents neurotoxins from entering into the brain parenchyma while at the same time blocking the entry of diagnostic or therapeutic agents1,2. Focused ultrasound (FUS) in conjunction with microbubbles (1–10 μm) is currently the only way to open the BBB noninvasively, locally, and transiently for drug delivery and treatment for central-nervous-system (CNS) diseases3. It has been shown to treat glioblastoma4,5 and Alzheimer’s disease6,7 in small animal models. More applications are to be explored as various agents as small as magnetic resonance contrast agents (gadolinium, ~200 pm) to as large as viral vectors (~20 nm)8,9, nanoparticles (~100 nm)10,11, and stem cells (~10 μm)12 have be reported to pass the BBB with this technique. It has been translated to large animals such as non-human primates (NHP)13,14, with short-term (hours to 9 weeks) and long-term (4 to 20 months) safety confirmed with MRI, histology, and behavioral assessment15,16. This technique is under clinical trials to treat patients with malignant brain tumors17,18.

To ensure an effective and safe BBB opening for drug delivery and treatment, cavitation associated with BBB opening could be monitored19. This association of cavitation with BBB opening lies on the physical mechanism for BBB opening. Cavitation occurs in the vessels in the focal area during the FUS treatment, and the oscillating bubbles generated an acoustic signature reflecting both the strength and type of bubble activity20,21. If bubbles oscillate periodically without disruption, harmonic (bubble volumetric oscillation; n*f, where n = 1, 2, 3, … and f = excitation frequency) and/or ultraharmonic signals (bubble shell oscillation; n*f + f/2, where n = 1, 2, 3, … and f = excitation frequency) will be generated, a phenomenon known as stable cavitation. At higher pressures when bubbles oscillate violently and collapse rapidly, shock waves and microjets are generated with broadband emission known as inertial cavitation. Both stable and inertial cavitation applies mechanical stress onto the vessel walls22,23 which could disrupt the tight junctions and other processes of BBB and increase the permeability of capillary endothelium24.

Passive cavitation detection (PCD) served as a tool for real-time transcranial monitoring during FUS, and could serve as an online treatment evaluation complement to the post-operative MRI-based methods. Since its initial use during BBB opening in small animals19, PCD has been expanded to various types of contrast agents such as microbubbles of various sizes25, various shells26, and nanodroplets27 reporting a positive correlation between BBB opening and cavitation dose. A feedback control mechanism for BBB opening based on ultraharmonics and subharmonics was established in small animals28,29. The permeability and reversibility of BBB opening could also be predicted based on cavitation in mice30. Nevertheless, the translation of these findings with PCD monitoring to primates remains in question due to the thicker skull and heterogeneous brain parenchyma significantly attenuating the cavitation signals.

Since the preliminary study demonstrating the feasibility of transcranial cavitation detection in NHPs in vivo14 and evidence that both stable and inertial cavitation doses could be monitored in real time through the intact NHP and human skulls31, PCD monitoring has been steered towards clinical applications as it was the only technique assessing the treatment effectiveness in real time32. However, cavitation monitoring in NHP for assessing BBB opening was found to be highly variable14 and was not correlated with the BBB opening outcome such as opening volume33, unlike in small animals such as rodents that showed good correlation. We hypothesized that this discrepancy may be due to the heterogeneous and gyrencephalic nature of the primate brain (i.e., gray and white matter, and large cerebral vessels) in primates or the targeting parameters. Furthermore, information critical in future clinical applications such as the amount of drug delivered and its associated delivery efficiency has yet to be established with caviation in vivo. These issues can be of high importance and therefore essential in the pre-clinical evaluation of the technique in NHP.

Therefore, the aims of this study were to determine (1) how the cavitation activity correlated with the BBB opening volume, the amount of model drug (gadolinium) delivered through the barrier and its delivery efficiency; (2) how the heterogeneous primate brain (gray and white matter, large vessels) influenced the cavitation activity and the BBB opening outcomes; (3) the targeting accuracy and effect to the cavitation monitoring. Real-time transcranial cavitation monitoring of the stable and inertial cavitation doses was performed in four macaque monkeys during sonication through stereotactic targeting. For the quantitative analysis of BBB opening volume and drug delivery efficiency, T1-weighted (T1w) imaging and variable flip-angle (VFA) T1 mapping in magnetic resonance imaging (MRI) were performed 1 h following BBB opening. These opening outcomes were then correlated with the acquired acoustic monitoring findings in order to assess the PCD predictability value among animals. The effect of gray and white matter on BBB opening and cavitation was analyzed after tissue segmentation. The vasculature effect on cavitation monitoring was evaluated by targeting a region proximal to the medial cerebral artery (MCA) with a monotonically increasing pressure for PCD calibration. The effect of the incidence angle with respect to the skull on cavitation monitoring was evaluated by varying the targeting angle with PCD calibration as well.

Results

Variability in BBB opening and cavitation monitoring

With the FUS system setup targeting the basal ganglia (Supplementary Fig. S1), the BBB opening in NHP was achieved with the opening volume and delivery efficiency quantified following the pipeline in Fig. 1 through pre- and post-contrast (Gd, gadolinium) T1w imaging and T1 mapping for Gd concentration ([Gd]c) as the Gd, a paramagnetic particle does not cross the BBB, perfused the BBB opening region and shortened the relaxation time of the tissue. Figure 2 showed representative BBB opening findings in two NHPs with acoustic monitoring of the cavitation dose (SCDh: stable cavitation dose with harmonics, SCDu: stable cavitation dose with ultraharmonics, ICD: inertial cavitation dose with broadband emission). BBB opening was revealed in both T1w imaging (Fig. 2A,B) and the [Gd]c map (Fig. 2C,D) in both gray and white matter (Fig. 2E,F) after a significant increase of SCDh (Fig. 2G,H). In NHP 2 (300 kPa on the right putamen), an opening volume of 298 mm3 was achieved with the highest [Gd]c in the opening area reached 0.07 mM, and the amount of Gd delivered was 10.7 nmol. The BBB opening volume was 393 mm3 in NHP 3 (600 kPa on the left putamen) with the highest [Gd]c reached 0.06 mM, and the amount of Gd delivered was 8.7 nmol. The [Gd]c was higher in gray matter than that in white matter in the BBB opening area. While comparing to the Gd retention of the unsonicated tissue (Table 1), the [Gd]c at the center of the opening was higher than that in the unopened brain parenchyma (0.01–0.02 mM), similar to that of the muscle (0.06–0.07 mM), and lower than that of the vessel (0.14–0.15 mM).

Figure 1: Pipeline for BBB opening volume quantification and drug delivery analysis based on MR image processing.
Figure 1

(A) To quantify BBB opening volume, the contrast enhancement map (upper: horizontal slice, lower: coronal slice) were used after dividing the post-Gd T1 images by the pre-Gd images. Then, after applying the brain mask and the vessel mask in order to filter out the contrast enhancement outside of the BBB opening area (B), the opening volume was calculated by subtracting the VOI in the contralateral area (dashed rectangle) from the targeted area (solid rectangle) (C). On the other hand, the pre- Gd T1,0 (D) and post-Gd T1 maps (E) were used to quantify the amount of Gd delivered and its delivery efficiency since the Gd shorted the T1 time after diffused the BBB opening region (arrowhead). The Gd concentration map (F) (upper: horizontal slice, lower: coronal slice) was acquired by calculating the change of T1 time between pre- and post-Gd T1 maps. After applying the brain mask and excluding the Gd retention in the vessels by thresholding (G), the amount of Gd delivered was calculated by subtracting the VOI in the contralateral area (dashed rectangle) from the targeting area (solid rectangle) (H).

Figure 2: BBB opening with real-time acoustic cavitation monitoring in two NHPs.
Figure 2

FUS-induced BBB opening in NHPs was visualized in T1w images after overlaying the contrast enhancement onto the post-Gd T1w image (A,B) (A: NHP 2 at 300 kPa; B: NHP 3 at 600 kPa). The corresponded Gd concentration map ([Gd]c) (C,D) showed the variation of Gd delivered in gray and white matter according to the tissue segmentation map based on the pre-Gd T1 map (E,F) (dark gray: gray matter, light gray: white matter, black: blood, white: CSF). The acoustic cavitation emission were recorded and calculated in real time, where SCDh denotes stable cavitation dose with harmonics, SCDu for stable cavitation dose with ultraharmonics, and ICD for inertial cavitation dose. (G,H). The BBB opening of NHP 2 and 3 showed an inter-animal variation and may be due to the skull.

Table 1: Gd retention in different tissues after Gd injection (without applying FUS).

Both intra- and inter-animal variation in BBB opening were observed in the quantitative results (Fig. 3). In repeated sonication in NHP 1 (275 kPa at putamen, Fig. 3A) to estimate intra-animal variation, the coefficient of variation in the opening volume was 22% (ratio of the standard deviation to the mean). Inter-animal variation was observed in the pressure threshold for BBB opening after applying an estimate of 50% pressure increase to compensate the skull attenuation. As shown in Fig. 3B, the pressure threshold for NHP 1 and 2 was lower (250 kPa) than that in NHP 3 (350 kPa) with the same range of vital signs during sonication (Supplementary Table S1). This discrepancy was also observed in the [Gd]c map analysis performed in NHP 2 and NHP 3 (Fig. 3C), showing that the amount of Gd delivered and the delivery efficiency in NHP 2 was higher than that in NHP 3 using the same pressure. This inter-animal variation may be due to the difference in skull and tissue attenuation, and can be corrected through simulation predictions based on the same animal’s CT34. Nevertheless, an opening volume of 400 mm3 and 15 nmol of Gd delivered (0.0015% of delivery efficiency) could be achieved.

Figure 3: Cavitation monitoring in assessing the BBB opening and drug delivery.
Figure 3

(A) In order to study the intra-animal variation, NHP 1 was sonicated with the same targeting (putamen) and acoustic parameters (275 kPa) in a bi-weekly basis five times. FUS-induced BBB opening was performed in three NHPs with the opening volume (B), and the drug delivery analysis was performed in two NHPs for the amount of Gd delivered and the delivery efficiency (C). NHP 1 to NHP 3 were sonicated 7, 12, and 24 times, respectively, and the error bar represents standard deviation. Both (B) and (C) showed an inter-animal variation as the BBB opening threshold for NHP 1 and 2 were lower than that for NHP 3. Quantified cavitation doses (SCDh: stable cavitation dose with harmonics, SCDu: stable cavitation dose with ultraharmonics, ICD: inertial cavitation dose) were correlated with the BBB opening outcomes. The SCDh can be used to detect the effectiveness of BBB opening (D). For a quantitative assessment, the opening volume (E), the amount of delivered Gd and delivery efficiency (F) was positively correlated with the total cavitation dose (SCDh + SCDu + ICD). (The R2 of the linear fitting in (E) for NHP 1 to 3 and all experiments across animals was 0.81, 0.63, 0.50, and 0.47, respectively; that in (F) for NHP 2, 3, and all experiments across animals was 0.52, 0.71, and 0.61, respectively.)

Cavitation monitoring characterized BBB opening without significant intra- and inter-animal variation, and could be used to predict BBB opening volume, amount of delivered Gd and its delivery efficiency. The cavitation monitoring was positively correlated with the BBB opening volume given the intra-animal variation (Fig. 3A), suggesting the possibility of using cavitation monitoring as a feed-back loop to control the FUS treatment in NHPs. Moreover, the SCDh (sensitive to bubble activity at low pressures) was found to be an indicator of the effectiveness of the FUS procedure among animals (Fig. 3D, p < 0.01). The total cavitation doses (SCDh + SCDu + ICD) was positively and linearly correlated with the opening volume (Fig. 3E, R2 = 0.47), amount of delivered Gd and delivery efficiency (Fig. 3F, R2 = 0.61), and could serve as a surrogate for MRI-based treatment evaluation. No significant difference was found among animals, while the R2 of each individual varied. The total cavitation doses was adopted since it best correlated with the opening outcomes as the SCDh and SCDu may reach a plateau at high pressures while the ICD was detected31. Transcranial cavitation monitoring showed insignificant intra-animal variation may be because the recorded cavitation signal reflected the in situ pressure after attenuation.

Effect of brain heterogeneity

The effect of brain heterogeneity on BBB opening, drug delivery, and cavitation monitoring was separated into two parts: the effect of gray and white matter, and the effect of large vessels. First, in studying the effect of gray and white matter (Fig. 4), three animals were sonicated at the caudate nucleus and putamen at pressures causing a BBB opening volume of 400 mm3 (300 kPa for NHP 2, and 450 kPa for NHP 3 and 4 due to the inter-animal variation). The opening volume in the caudate nucleus was the same or slightly smaller than that in the putamen in the same animal without statistical significance (Fig. 4A), and the cavitation response revealed the same trend (Fig. 4B). Although the gray-and-white matter composition in the sonicated area was about 1:1 (Fig. 4C), the gray matter contributed 68.8% of the BBB opening in comparison to 21.4% for the white matter (Fig. 4D), meaning that the gray matter had the probability of BBB opening three times higher than that of the white matter. In order to investigate the capability of cavitation monitoring on the gray and white matter, their opening volume were delineated for correlating with the total cavitation dose. As shown in Fig. 4E, a better correlation of cavitation dose to the total opening volume was found (R2 = 0.51, p < 0.001) compared with the volume of gray matter only (R2 = 0.15, p = 0.05), suggesting that the cavitation was detected in both the gray and white matter and caused BBB opening in both.

Figure 4: Effect of gray and white matter.
Figure 4

FUS was applied in the caudate and putamen in three animals causing a BBB opening volume close to 400 mm3 (300 kPa for NHP 2, 450 kPa for NHP 3 and 4). The opening volume was shown in (A), and the total cavitation dose in (B). Based on the tissue segmentation, the gray-and-white matter composition in the sonicated area (C) and in the BBB opening area (D) were calculated. The correlation of total cavitation dose to the BBB opening volume was calculated in (E), in which it was better correlated with the total opening volume than with the volume in gray matter.

Second, the effect of large vessels (diameter ≥1 mm) in the BBB opening and cavitation monitoring were investigated by targeting a region (putamen) close or including the middle cerebral artery (MCA) in NHP 4. As shown in the MRA (Fig. 5A–C), the NHP brain is rich in cerebral blood vessels of different sizes. When applying FUS at 450 kPa, the BBB opening was found to be successful regardless of the distance to MCA in the four cases in Fig. 5D (opening volume: 309, 469, 443, and 758 mm3 and angle of incidence to the skull: 24°, 18°, 35°, 41° for case i to iv, respectively). However, the cavitation response was significantly enhanced as the focus drew closer to the MCA. Interestingly, the spontaneous SCDu and ICD were found to be reliably detected when targeting a region including MCA, and a periodicity of the cavitation level was similar to the breathing rate in certain cases where large vessels were in the focus. This increased cavitation response was also observed in the PCD calibration assessing the cavitation level (the cavitation dose of one single pulse) at different pressures (Fig. 5E–G) after the BBB opening in Fig. 5D. The ICD increased monotonically with pressure (Fig. 5G) while the SCDh (Fig. 5E) and SCDu (Fig. 5F) increased until a plateau was reached at higher pressures. Furthermore, although the opening volume in cases ii and iii were similar, the cavitation level of SCDh, SCDu, and ICD in the PCD calibration followed a different variation. The SCDh was slightly lower at pressures above 300 kPa while the SCDu and ICD were higher in case iii (MCA in the focus) compared to case ii (Fig. 5D).

Figure 5: Vasculature effect.
Figure 5

MR angiography (MRA) in NHP in (A) coronal, (B) sagittal, and (C) horizontal view, and the middle cerebral artery (MCA) was indicated by an arrowhead. (D) Four BBB opening cases targeting regions proximal to the MCA (arrowhead) at 450 kPa in NHP 4, where the upper row showed the opening volume overlaying onto the post-Gd T1w images and the lower row was the cavitation response along the sonication time. PCD calibration (E–G) after BBB opening in cases i-iii was performed in order to assess the cavitation level at different pressures (10 pulses per pressure) with targeted regions near or include the MCA (E: SCDh, F: SCDu, G: ICD), and the errorbar represented the standard deviation of the 10 sonications. Note that the opening volume for case i to iv was 309, 469, 443, and 758 mm3 and angle of incidence to the skull: 24°, 18°, 35°, 41°, respectively. The cavitation level varied as the targeted region approached the MCA that was correlated with the opening volume but was found to be independent of the incidence angle.

Targeting accuracy and the effect to cavitation monitoring

Following targeting planning in the caudate and putamen, the resulting BBB opening was visualized in brain in the 2D coronal plane (Fig. 6A), in the stacked 2D horizontal planes (Fig. 6B) and in 3D through the skull (Supplementary Fig. S2). The targeting accuracy was quantified in four animals (Fig. 6C) based on the defined FUS orientation (axial and lateral distance shift relative to the planned FUS trajectory) and the angle relative to the skull. The lateral and axial shift ranged between 0.9 mm to 6.4 mm and 1.0 mm to 7.6 mm. When targeting the caudate, the overall shift ranged between 2.4 mm to 6.5 mm with an average of 3.7 mm, and the angle shift was 8.1° with an averaged incidence angle of 5.0° and refraction angle of 7.1°. When targeting the putamen, it ranged between 4.4 mm to 10.1 mm with an average of 7.5 mm, and the angle shift was 7.3° with an averaged incidence angle of 14.2° and refraction angle of 15.8°.

Figure 6: Targeting accuracy and the effect on cavitation monitoring.
Figure 6

(A) Visualization of targeting in the caudate nucleus (dashed contour in the left column) and the putamen (dashed contour in the right column) and the BBB opening by overlaying the contrast enhancement onto the post-Gd T1w image, where * denotes the centroid of BBB opening. (B) Targeting trajectory and the opening trajectory showed in the stacked horizontal slices. (C) Target shift in distance (top) and angle (down), where lPut represents left putamen, rPut for right putamen, lCd for left caudate, and rCd for right caudate. (D) The effect of incidence angle to the cavitation response in NHP 2, where the legend denotes targeted structure and the incidence angle. The error bar represents standard deviation.

The effect of the incidence angle on the cavitation monitoring (Fig. 6D) was investigated by performing PCD calibration in NHP 2 with varied incidence angle targeting at basal ganglia (right caudate and right putamen with the same tissue composition in both sonicated region and BBB opening region). The SCDh was used since it was reliably detected at the pressures of 100 kPa to 600 kPa. With a lower incidence angle targeting at the right caudate (incidence angle: 6.6°), the SCDh was higher than that for the right putamen (incidence angle: 21.8°). However, the difference was statistically insignificant due to the intra-animal variation, suggesting an insignificant effect of transducer orientation to cavitation monitoring.

Safety

The safety of the FUS procedure was evaluated in all experiments using MRI in 1 h (edema in hyper-intensity of T2w imaging, and hemorrhage in hypo-intensity of SWI). No edema, hemorrhage, or any kind of macroscopic damage was detected in any of the animal in this study. Representative cases with large BBB openings in the four animals were shown in Fig. 7. For NHP 1, one sonication was performed at 275 kPa (putamen, opening volume: 397 mm3), two for NHP 2 at 400 kPa (caudate and putamen, opening volume: 783 mm3 and 436 mm3, respectively), one for NHP 3 at 450 kPa (putamen, opening volume: 845 mm3), and two for NHP 4 at 600 kPa (caudate and putamen, opening volume: 623 mm3 and 539 mm3, respectively). In addition, no damage was detected in the studies of the vasculature effect (Fig. 5) despite the high cavitation response. The damage was not detectable but, if present, might be microscopic and insignificant in histological examination for sonications without detectable damage in MRI14.

Figure 7: Safety assessments in the MRI.
Figure 7

(A) NHP 1 targeting putamen at 275 kPa. (B) NHP 2 targeting caudate nucleus and putamen at 400 kPa. (C) NHP 3 targeting putamen at 450 kPa. (D) NHP 4 targeting caudate nucleus and putamen at 600 kPa. No edema or hemorrhage was detected in any of the cases. 1st row: contrast enhancement of the BBB opening overlaying onto the post-Gd T1w imaging; 2nd row: T2w imaging for edema; 3rd row: susceptibility-weighted imaging (SWI) for hemorrhage.

Discussion

Our findings presented herein have provided a link between the transcranial cavitation detection for assessment of the BBB opening and the drug delivery outcomes in non-human primates through the use of both stable and inertial cavitation dose. The findings demonstrated a clear relationship between the cavitation measures and the BBB opening characteristics in non-human primates such as the opening volume, the drug amount delivered and the delivery efficiency. Cavitation was detected in both gray and white matter and was correlated with the BBB opening in both, while the cavitation response varied by the large cerebral vessels due to the change of cavitation threshold.

Several new findings have been shown in this study in comparison to previous NHP studies. First, cavitation monitoring was shown to be highly correlated with clinically relevant measures in the deeply seated subcortical structures, an improvement compared with the previous study using relative MRI enhancement in the cerebral cortex32. Second, cavitation monitoring was capable of detecting the delivery in both gray and white matter, while large vessels (diameter >1 mm) resulted in saturated stable cavitation response and significantly higher inertial cavitation response at high pressures without detectable damage in the MRI. Third, the cavitation quantification using harmonics, ultraharmonics, and broadband emission could improve the correlation to the BBB opening and drug delivery via compensation of the effect of brain heterogeneity and the nonlinear skull effect. Although harmonics can be more easily detected, they could be hindered by nonlinear skull or large vessel effects at higher pressures and deteriorate the correlation with drug delivery and BBB opening. The primate skull is reported to contribute to harmonic signals at higher pressures, which decreased the cavitation signal-to-noise ratio31. Furthermore, harmonics from bubbles in the large vessels could reach a plateau at higher pressures (Fig. 5). These could be the reason why the use of harmonics has previously failed in predicting the BBB opening volume in NHP33, and that harmonics were correlated well mostly in the cerebral cortex in a small juvenile monkey with fewer large vessels and thinner skull producing insignificant skull effects32.

Inertial cavitation reported to be indicative of vascular damage15 or using ultraharmonics to control the treatment28 should be considered with caution in large animals, since the large vessels can result in high ultraharmonics and broadband emission without detected damage in radiologic examination implying no significant damage in the histological examination15. This vascular effects in humans could prove even more important due to the larger sizes of cerebral vessels compared to smaller animals (e.g. the cerebral artery diameter for mice, monkey, and human is 0.2 mm35, 1.2 mm36, and 2.0 mm37, respectively). The reason behind variable cavitation monitoring may lie in the fact that the microbubbles circulating in large vessels responded differently to FUS. As has been reported previously, the threshold of inertial cavitation was lower in larger vessels38,39, so more bubbles may be disrupted in large vessels without stable cavitation, resulting in the decrease of SCDh and SCDu at higher pressures with a monotonically increase of the ICD. Since larger vessels allow microbubbles to nonlinearly oscillate, the energy of volumetric oscillation (SCDh) may convert to shell oscillation (SCDu) and violent bubble oscillation leading to bubble collapse (ICD)21. The high broadband emission in this case may not cause vascular damage as the microjets may exert negligible forces towards the vessel wall due to the larger lumen space. Furthermore, large vessels composed of thicker walls and muscles may not easily be disrupted. Therefore, large vessels should be excluded from the FUS focal region for reliable cavitation monitoring.

For molecular delivery to the heterogeneous brain, the BBB opening results showed a localized delivery in both the contrast enhancement and the [Gd]c map (Fig. 2). The highest [Gd]c after BBB opening was the same as that of the epicranial muscle (Table 1), since the highest tissue permeability after BBB opening was comparable to that of tissue without BBB40. Moreover, the highest delivery ([Gd]c) occurred only at the center of the focus given a larger opening volume (298 or 393 mm3) with a shape similar to that of the transducer focus. This suggests that the technique is suitable for region- or point-specific delivery with the highest efficiency, and the localization can be further improved using chirp coded acoustic pulses41. If a higher amount of delivery is required, increasing the injection dosage of the drugs could enhance the amount of delivery42. If a larger opening volume is desired, sonication at multiple locations would be necessary. However, the BBB opening occurred with greater ease in the gray than in the white matter15 was confirmed in this study. BBB opening occurred in both gray and white matter with the probability in the gray matter three times higher than that for the white matter (Fig. 4), and the drug concentration (Gd) was significantly higher in the gray matter as well (Fig. 2). This may be due to the lower vascularity or higher attenuation in the white matter than that in the gray matter43. Moreover, since cavitation could be monitored in both tissue types, this discrepancy may be associated with blood perfusion differences during sonication. For example, the blood volume (1.5–1.8 times44,45) or blood flow (1.7 times46) in the gray matter was higher than that of the white matter. Future studies regarding this issue would be required in optimizing this technique for various tissue types.

In addition, the BBB opening was achieved targeting a region including the large vessel MCA (Fig. 5). However, since microbubbles in circulation would attenuate ultrasound wave propagation at varying extents depending on the vessel distribution, size, and microbubble concentration47, this microbubble shielding effect may impose potential difficulties in humans17 as it was reported to hinder BBB opening in monkeys14. Therefore, large cerebral blood vessels should be avoided in the targeted region or acoustic path during FUS planning in order to avoid opening failure as well as monitoring discrepancies. MRA could be a useful tool for visualizing the position and size of cerebral blood vessels during the pre-planning process. Furthermore, the use of nanodroplets to induce BBB opening could potentially minimize the shielding effect since nanodroplets vaporize into bubbles only in the focus and induce opening, which also strongly decreases variability in the relationship of cavitation dose to BBB opening27.

Modeling bubble dynamics could potentially provide a priori knowledge for the safety and efficacy of BBB opening and drug delivery in various tissue types, as the mechanical effects of cavitation have long thought to associate with vascular permeability enhancement. A proper model is thus required for these emerging therapeutic purposes. Currently, modified or lumped-parameter models based on the Rayleigh-Plesset equation (free gas bubble model) have enabled us to investigate the behavior of coated microbubbles48,49 and the bubble-microvessel wall effects50, assuming symmetric bubble oscillating in free space. However, a finite-element modelling (FEM) approach provides flexibility for both symmetric and asymmetric bubble oscillation while considering a variety of bubble and in vivo environmental properties51. Despite the findings obtained with this modelling approach, many efforts are also required to build a linkage to the in vivo BBB opening and drug delivery. First, the mechanical effects directly associated with the therapeutic effects need to be identified. Second, the in vivo microbubble properties must be measured, including the size, shell25,52, stiffness53, and distribution of bubbles as well as the shape, size, and wall elasticity of the microvessels. Third, the bubble-to-bubble interaction and the bubble behavior after rupture49.

In this study, the targeting shift in the putamen was higher than in the caudate (Fig. 6). Both the gyrencephalic brain and the incidence angle could play an important role as more layers of gyrus and sulcus were included in the beam path when targeting the putamen with larger incidence angles. Heterogeneous tissue in the acoustic beam path may affect the targeting accuracy due to the refraction between layers and the heterogeneous BBB opening in the gray and white matter affecting the analysis. Higher incidence angles could cause larger shifts due to the wave distortion in the skull54 and refraction between the skull layer as well (refraction angle > incidence angle). Besides, the stereotactic arm holding the heavy FUS transducer may cause shift while targeting with a large angle deviating from the mid-sagittal plane.

Conclusion

The study presented herein expanded the role of transcranial cavitation monitoring in NHPs in vivo and demonstrated a correlation with the BBB opening volume and the drug delivery efficiency with various the tissue types and targeting. The achieved BBB opening volume, amount of delivered molecules and delivery efficiency was as high as 800 mm3, 40 nmol, and 0.004%, respectively, and could be predicted by the real-time cavitation monitoring. Quantitative cavitation monitoring and drug delivery were achieved in both the gray and white matter, with the probability of successful BBB opening three times higher in the gray matter than in the white matter. The average targeting shift was 3.7 mm in the caudate and 7.5 mm in the putamen, and the incidence angle to the skull had negligible effects on the cavitation monitoring, showing the capability of cavitation monitoring. The large cerebral vessels, however, may affect cavitation monitoring, and should be avoided in different targets.

Methods

Preparation of Animals

In accordance with the National Institutes of Health Guidelines for animal research, all procedures were reviewed and approved by the Institutional Animal Care and Use Committee at Columbia University and the New York State Psychiatric Institute. Four male rhesus macaques (Macaca mulatta, weight: 7–10 kg, age: 8–20 yo) were used in this study. Each animal was sedated with ketamine (5–15 mg/kg in conjunction with 0.04 mg/kg of atropine through intramuscular injection) for placement of an endotracheal tube and an intravenous catheter in the saphenous vein, and was under anesthesia using 1–2% isoflurane-oxygen mixture with vital signs (electrocardiography, heart rate, blood pressure, SpO2, breathing rate, end-tidal CO2) monitored during the entire experiments. No animals were euthanized in this study.

Ultrasound System

The system setup is shown in Supplementary Fig. S1. For sonication with FUS, a spherically-focused single-element transducer (H-107, Sonic Concepts, WA, USA) operating at 0.5 MHz (full-width-at-half-maximum focal size: 5.85 mm in width and 34 mm in depth, geometric focal depth: 62.6 mm) was used. For real-time monitoring of the acoustic cavitation emission (passive cavitation detection, PCD), a spherically focused, flat-band, polyvinylidene fluoride (PVDF) hydrophone (Y-107, Sonic Concepts, WA, USA), coaxially and confocally aligned with the FUS transducer, served as the passive cavitation detector. A PC workstation (model T7600, Dell) with a customized program in MATLAB® (Mathworks, MA, USA) was developed to automatically control the sonication through a programmable function generator (model 33220 A, Agilent Technologies, CA, USA) and a 50-dB amplifier (A075, ENI, NY, USA). The PCD signal acquisition was performed with a 14-bit analog-to-digital converter (Gage Applied Technologies, QC, Canada) (sampling rate: 50 MHz) after 20-dB amplification. The PCD signals were monitored transcranially in real time including the frequency spectra and cavitation doses, which were reported to be detectable with the same system and method at pressure as low as 100 kPa in vivo through the NHP skull31.

Experimental Procedures

A stereotaxis-based method developed previously33 was used for targeting the dorsal striatum (caudate and putamen) (Supplementary Fig. S1), deep subcortical structures associated with neurodegenerative diseases such as Parkinson’s disease. The pressures at the focus of the FUS transducer were calibrated using a bullet hydrophone through ex vivo rhesus macaque skulls, and 50% of pressure loss due to the skull was compensated in the in vivo experiments55. The in-house made, lipid-shelled, monodisperse microbubbles (4–5 μm in diameter)56 were freshly diluted to 3 mL with a dosage of 2.5 × 108 bubbles/kg of body weight. Another 3 mL of saline was used to flush after microbubble injection. At the beginning of the FUS procedure, the control sonication (duration = 5 s) was performed before microbubble injection as a baseline for cavitation monitoring, and then the microbubbles were injected in a bolus intravenously (saphenous vein) followed by saline flush within 30 s while the sonication started at the same time (peak negative pressure = 200–600 kPa, pulse length = 10 ms, pulse-repetition frequency = 2 Hz, duration = 2 min). A second sonication was performed in 11 out of a total of 47 experiments at a second non-overlapping target 20 min after the microbubbles were eliminated from the first experiment.

In order to investigate the effects of targeting and heterogeneous brain, PCD calibration (N = 31) (a 5-s consecutive sonication at monotonically increasing pressures ranging within 100–600 kPa starting at 10 s after re-injecting half the dose of microbubbles)31 was performed after the regular sonication targeting a region proximal to the medial cerebral artery (MCA). After the FUS procedure, the animal was transferred to the MRI suite for assessing the BBB opening and safety within 1 h. Two sham cohorts were also performed by applying FUS without injecting microbubbles (FUS +/MB−, N = 4) or without FUS procedure (FUS−/MB−, N = 3) for comparison to determine BBB opening in the experimental groups.

Magnetic Resonance Imaging Acquisition

A 3.0 T MRI system (Achieva, Philips Medical Systems, USA) with an eight-channel head coil was used for assessing safety and BBB opening. T1w imaging was used for BBB opening detection and opening volume quantification because of its higher sensitivity, while T1 mapping was for quantifying the amount of delivered MR contrast agents and the delivery efficiency. For assessing BBB opening, both pre- and post- contrast agent T1-weighted (T1w) images and T1 maps using the variable flip-angle (VFA) SPGR method were acquired. The contrast agent used in this study was Gd-DTPA-BMA as the model drug (gadodiamide or Gd, molecular weight = 573.66 Da; Omniscan®, GE Healthcare, NJ, USA) with the same dosage suggested for patients (0.2 mL/kg or 0.1 mmol/kg of body weight). Pre- and post-Gd (40 min after injection) T1w images using 3D spoiled gradient echo (SPGR) sequence (TR/TE = 8.5/4.8 ms, FA = 8°, SR = 0.97 × 0.97 × 1 mm3) were acquired for detecting the opening and analyzing the opening volume. For quantitative analysis of the Gd concentration and delivery efficiency, pre- and post-Gd (20 min after injection) T1 maps were acquired using a series of 3D SPGR sequence with five flip angles (TR/TE = 10/4 ms, FAs = 5°/10°/15°/20°/35°, SR = 0.89 × 0.89 mm2, SL = 1 mm). For detecting edema, T2-weighted (T2w) images were acquired using 3D Turbo Spin Echo sequence (TR/TE = 3000/80 ms, flip angle or FA = 90°, resolution = 0.42 × 0.42 × 2 mm3). For detecting hemorrhage, susceptibility-weighted imaging (SWI) was performed (TR/TE = 19/27 ms, FA = 15°, resolution = 0.44 × 0.44 × 1 mm3). Magnetic resonance angiography (MRA) was conducted in order to visualize the size and orientation of blood vessels using 3D time-of-flight SPGR sequence (TR/TE = 23/3.4 ms, FA = 15°, resolution = 0.89 × 0.89 × 1 mm3) in a separate 3.0 T scanner (Signa, GE Healthcare, USA) with a customized two-channel head coil.

Quantification for BBB Opening

The BBB opening volume was quantified using pre- and post-Gd T1w images in Matlab with custom-built programs31. In brief, both pre- and post-Gd images were first registered to the individual stereotactically-aligned images (IST) with FSL’s FLIRT toolbox57, computing the ratio of post- to pre-Gd images as a measurement of contrast enhancement, which was normalized by linear scaling with reference to the unsonicated thalamus and the anterior cerebral artery (ACA) as shown in dashed and solid circle in the horizontal slice (Fig. 1A), respectively. In order to filter out the background contrast enhancement in the cerebral vessels and muscle tissue outside the brain for quantifying the BBB opening volume, the brain mask was applied (generated using pre-Gd T1w images from the no-FUS sham cohort with FSL’s Brain Extraction Toolbox58) and the enhancement images of the FUS−/MB- sham cohort for each individual was subtracted from the enhancement images (coronal slice in Fig. 1A), giving rise to Fig. 1B. Finally, the opening volume was calculated by applying a volume of interest (VOI, 10 × 10 × 32.5 mm3) on the targeted region (solid box) subtracting the VOI on the contralateral side (dashed box) as shown in Fig. 1C. The threshold of BBB opening (80 mm3) was defined by the average opening volume plus 3 times the standard deviation in the FUS + /MB− sham cohort.

Similarly, the Gd concentration maps42,59 provide quantification of the Gd amount that crossed the BBB. The delivery efficiency was calculated using the pre- (Fig. 1D) and post-Gd T1 maps (Fig. 1E)59. First, the standard line fit method of VFA SPGR60 was used to calculate the pre- and post-T1 maps after registering the 3D SPGR images of various flip angles to the IST. Then, the Gd concentration map (Fig. 1F) was generated based on the following equation:

where [Gd]c is the Gd concentration, r1 is the relaxivity of the contrast agents (4 s−1mM−1 for Gd-DTPA-BMA61), T1 is the post-Gd T1 time, and T1,0 is the pre-Gd T1 time. Note that in the [Gd]c map (Fig. 1F), the concentrations in the unopened brain tissue (thalamus, dashed circle), temporalis muscle (black solid circle), and anterior cerebral vessels (ACA, white solid circle) were also calculated and compared with that of the BBB opening area. In order to filter out the [Gd]c in the cerebral vessels and muscle tissue outside the brain for quantifying the delivered Gd due to BBB opening, the [Gd]c maps (coronal slice in Fig. 1F) were thresholded by setting the concentration higher than that in ACA to zero and applying the brain mask, giving rise to Fig. 1G. Finally, the amount of delivered Gd was calculated based on equation (2) by applying a volume of interest (VOI, 10 × 10 × 32.5 mm3) on the targeted region (VOIipsi, solid box in Fig. 1H) subtracting the VOI on the contralateral side (VOIcontra, dashed box in Fig. 1H) for excluding the intrinsic Gd retention:

where [Gd]BBB is the amount of delivered Gd (mole) and V is the voxel volume in the T1 map. The delivery efficiency was defined as the percentage of the amount of delivered Gd to the total amount of injected Gd (estimated to be 1 mmol).

The opening volume was separated by the tissue type (i.e., the gray matter, white matter, blood, cerebral spinal fluid) segmented based on the T1 time in the pre-Gd T1 map. The T1 time for blood is between 1 ms to 700 ms, 700 ms to 1170 ms for white matter, 1170 ms to 1800 ms for gray matter, and 1800 ms to 5000 ms for cerebral spinal fluid based on our measurement in the NHP and the previous study in humans60.

Analysis of targeting accuracy

The targeting accuracy was analyzed by comparing the planned targeting to the quantified BBB opening results using the T1w images as described in the previous section. The planned targeting was composed of a targeting vector with a focus and an approaching direction (trajectory) for an interested brain structure, as well as the angle of incidence to the skull. This incidence angle was defined as the angle between the targeting vector and the vector normal to the outer surface of the skull (Supplementary Fig. 2), which was estimated using the 3-D gradient field in the skull mask after the skull extraction on the pre-Gd T1w images with the Brain Extraction Toolbox in FSL62. The BBB opening vector was subsequently defined by the 3-D center of mass in the BBB opening in the VOI described previously and the vector from the 3D linear curve fitting of the 2-D center of mass in each of the horizontal slices, respectively. Similar to the incidence angle, the refraction angle was the angle between the BBB opening vector and the vector normal to the inner layer of the skull in the 3D gradient field. Finally, the target shift was defined as the distance between the center of focus in the targeting vector and the center of mass in the BBB opening, and the lateral and axial shift was the shift perpendicular and parallel to the targeting vector, respectively.

Quantification of Acoustic Cavitation Emission

Based on the method previously proposed31, stable cavitation dose with harmonics (SCDh), stable cavitation dose with ultraharmonics (SCDu), and the inertial cavitation dose (ICD) representing volumetric oscillation, shell oscillation, and drastic bubble oscillation to bubble collapse, respectively, were quantified separately. The frequency spectrum of the PCD signal (in volts) from each pulse (1.25–5.00 MHz) was separately filtered to extract the harmonics (maxima in the bandwidth of 20 kHz around the harmonic frequency n*f, where f = 0.5 MHz and n = 3, 4, 5, …. 10), ultraharmonics (maxima in the bandwidth of 20 kHz around the ultraharmonic frequency m/2*f, where f = 0.5 MHz and m = 5, 7, 9, …. 19), and broadband emissions (signal after suppressing the harmonics and the ultraharmonics; 360 kHz around the harmonic frequency and 100 kHz around the ultraharmonic frequency, respectively), and the root-mean-squared (RMS) amplitude of the filtered PCD signal from one pulse () was defined as the cavitation level (dCD, representing dSCDh, dSCDu or dICD), i.e., the cavitation dose of a single pulse. The cavitation dose of the entire sonication (CD, representing either SCDh, SCDu or ICD) was thus equal to the sum of the cavitation levels over the entire sonication time:

where t is the time for each pulse and T the total sonication duration.

Statistical Analysis

The two-tailed Student’s t-test or an unsupervised linear regression fit were performed using GraphPad Prism (La Jolla, CA, USA) for quantitative PCD in assessing the BBB opening outcomes. Statistical significance was defined with p-value < 0.05.

Additional Information

How to cite this article: Wu, S.-Y. et al. Characterizing Focused-Ultrasound Mediated Drug Delivery to the Heterogeneous Primate Brain In Vivo with Acoustic Monitoring. Sci. Rep. 6, 37094; doi: 10.1038/srep37094 (2016).

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Acknowledgements

This study was supported in part by the National Institutes of Health (R01 AG038961, R01 EB009041), the FUS Foundation, the Alfonso Martin Escudero Foundation and Pedro Barrie de la Maza (C.S.S.). The authors wish to thank the laboratory members and collaborators at Columbia University, New York Presbyterian Hospital, and New York State Psychiatric Institute for assistance: Marilena Karakatsani for the experiments, Wenlan Zheng for the analysis, Chad Samaroo with MRI scanning; Girma Asfaw for advices in animal anesthesia; Feng Liu (PhD) and Yunsuo Duan (PhD) for acquiring the MRA.

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  1. Department of Biomedical Engineering, Columbia University, New York, New York, USA

    • Shih-Ying Wu
    • , Carlos Sierra Sanchez
    • , Gesthimani Samiotaki
    • , Amanda Buch
    •  & Elisa E. Konofagou
  2. Department of Neuroscience, Columbia University, New York, New York, USA

    • Amanda Buch
    •  & Vincent P. Ferrera
  3. Department of Radiology, Columbia University, New York, New York, USA

    • Elisa E. Konofagou

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Contributions

S.-Y.W. and E.E.K. designed the experiments and wrote the manuscript. S.-Y.W., C.S.S., A.B. and G.S. performed the experiments. S.-Y.W., C.S.S. and G.S. analyzed the data. V.P.F. facilitated the experiments. All authors provided editorial comments on the manuscript.

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The authors declare no competing financial interests.

Corresponding author

Correspondence to Elisa E. Konofagou.

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