A defining characteristic of HIV-1 infection is the ability of the virus to persist within the host. Specifically, MHC-I downregulation by the HIV-1 accessory protein Nef is of critical importance in preventing infected cells from cytotoxic T-cell mediated killing. Nef downregulates MHC-I by modulating the host membrane trafficking machinery, resulting in the endocytosis and eventual sequestration of MHC-I within the cell. In the current report, we utilized the intracellular protein-protein interaction reporter system, bimolecular fluorescence complementation (BiFC), in combination with super-resolution microscopy, to track the Nef/MHC-I interaction and determine its subcellular localization in cells. We demonstrate that this interaction occurs upon Nef binding the MHC-I cytoplasmic tail early during endocytosis in a Rab5-positive endosome. Disruption of early endosome regulation inhibited Nef-dependent MHC-I downregulation, demonstrating that Nef hijacks the early endosome to sequester MHC-I within the cell. Furthermore, super-resolution imaging identified that the Nef:MHC-I BiFC complex transits through both early and late endosomes before ultimately residing at the trans-Golgi network. Together we demonstrate the importance of the early stages of the endocytic network in the removal of MHC-I from the cell surface and its re-localization within the cell, which allows HIV-1 to optimally evade host immune responses.
The human immunodeficiency virus type 1 (HIV-1) encodes a class of proteins that lack any known enzymatic activity. These proteins, termed “accessory proteins”, include Nef, Vpr, Vpu and Vif. Accessory proteins can promote viral fitness by allowing infected cells to evade the host immune response1. The ability of Nef to promote HIV-1 immune evasion has been ascribed to its extensive interaction network with host proteins2,3,4. Indeed, Nef interacts with multiple proteins implicated in membrane trafficking in order to downregulate cell surface levels of major histocompatibility complex class I (MHC-I), resulting in a decreased ability of infected CD4+ T-cells to be detected and killed by CD8+ cytotoxic T lymphocytes (CTLs)5. This rerouting of MHC-I away from the cell surface is an example of a viral protein usurping host cell functions to ensure viral replication.
Currently, two models explain how Nef orchestrates the re-localization of MHC-I away from the cell surface (reviewed in ref. 6). The first model, termed the signaling mode of downregulation, is activated early during infection and involves the targeting of Nef to the trans-Golgi network (TGN) by the host membrane trafficking regulator protein phosphofurin acidic cluster sorting protein 2 (PACS-2)2,7. Once at the TGN, Nef binds and activates specific Src-family kinases (SFKs), which subsequently trigger the phosphoinositide 3-kinase (PI3K)-dependent endocytosis of cell surface MHC-I3. Internalized MHC-I is then sequestered in an intracellular compartment by a process involving the cytoplasmic tail of MHC-I8 and the membrane trafficking regulator phosphofurin acidic cluster sorting protein 1 (PACS-1), which has previously been identified to interact with the membrane adaptor protein-1 (AP-1)2,3,9. Interestingly, Nef, AP-1 and MHC-I have been described to form a ternary complex which depends on the cytoplasmic tail of MHC-I10. Structural information obtained by Jia et al. revealed that residues Y320 and D327 in the cytoplasmic tail of MHC-I bridge key interactions with Nef and AP-1, supporting Nef-dependent downregulation of MHC-I10.
The second model of Nef-dependent MHC-I downregulation, termed the stoichiometric mode, occurs at later stages of infection7. In this model, Nef interacts with the cytoplasmic tail of MHC-I and traffics the receptor to a degradative compartment in a process that also involves the membrane trafficking regulators AP-1 in addition to coat protein 1 (COPI)11. The signaling and stoichiometric models are not mutually exclusive and appear to be temporally linked7. Moreover, the signaling model may be more prominent in T-cells, as they have a relatively short half-life, whereas, the stoichiometric model may be more relevant in longer lived monocytes7.
Despite having identified multiple membrane trafficking regulator proteins implicated in the removal of MHC-I from the cell surface, it remains unknown what cellular compartments are used by Nef during this process precluding our understanding of the pathway subverted by HIV-1 to evade immune surveillance. In addition, prior analysis of pathways implicated in Nef-dependent MHC-I downregulation have primarily relied on the co-localization of Nef or MHC-I with markers of the membrane trafficking apparatus without analyzing Nef and MHC-I in complex, which is essential for Nef’s ability to downregulate MHC-I12,13. Importantly, the various compartments implicated in the trafficking of receptors such as MHC-I play distinct functional roles within the endosomal trafficking system2. Vesicles, such as early and late endosomes, are often implicated in the movement of cargo from the plasma membrane to distinct subcellular locations14,15. Moreover, late endosomes can also facilitate trafficking of cargo to degradative lysosomal compartments16. In parallel, recycling endosomes will continuously deplete proteins from the cell surface and return them to this location17. Fortunately the identity of the various intracellular compartments that comprise the endosomal trafficking system can be distinguished by specific effector molecules that coat the cytosolic face of these vesicles18.
We have previously localized interactions at the subcellular level between Nef and membrane trafficking regulators PACS-1 and PACS-2 using bimolecular fluorescence complementation (BiFC)2. This technique is used to study protein-protein interactions within cells and involves the reconstitution of a visible fluorophore from split fluorescent molecules expressed as fusion proteins on two distinct putative protein binding partners19,20,21. BiFC has enabled us and others to identify locations inside the cell where Nef can interact with itself or cytosolic binding partners, such as the trafficking regulator sorting nexin 18 (SNX18)21,22,23.
In the current report, we sought to determine the trafficking route undertaken by MHC-I in complex with Nef, in order to determine the fate of MHC-I in Nef-expressing cells. Using BiFC, we demonstrate that the Nef:MHC-I interaction is dependent on key residues in the cytoplasmic tail of MHC-I and we localize the Nef:MHC-I interaction within cells. Specifically, we show that Nef interacts with MHC-I in both early and late endosomes, and at the trans-Golgi network, but that the Nef:MHC-I interaction is not detectable in lysosomes. Interestingly, we show that Nef depletes the amount of MHC-I in Rab11 positive recycling endosomes and that a functional early endosomal compartment is required for Nef-dependent MHC-I downregulation. This Nef-mediated rerouting eventually sequesters MHC-I at the trans-Golgi network. Taken together, these results, demonstrate for the first time, the specific endocytic compartments utilized by Nef to orchestrate MHC-I downregulation and support a model that results in the sequestration of MHC-I by Nef in a non-degradative compartment.
Bimolecular fluorescence complementation microscopy detects a Nef:MHC-I complex in cells
The immunoevasive capabilities of HIV-1 are largely mediated by the ability of Nef to remove MHC-I from the cell surface24. The crystal structure of a Nef:MHC-I complex revealed that this interaction is stabilized by AP-1, demonstrating that Nef, MHC-I and AP-1 are able to form a ternary complex24. To validate the Nef:MHC-I interaction in cells, we performed a bimolecular fluorescence complementation (BiFC) assay. BiFC entails the expression of a split Venus fluorophore from two distinct plasmids in the form of fusion proteins, and results in a reconstituted, functional Venus fluorophore when the two fusion proteins are within 100 nm20. Co-transfection of HeLa cells with plasmids encoding Nef-VC and MHC-I-VN-Flag, more specifically Nef and MHC-I fused to carboxy (VC) or amino (VN) fragments of Venus, respectively, revealed that Nef and MHC-I form a complex (Fig. 1a). In order to test for the requirement of AP-1 in the formation of the Nef:MHC-I complex in cells, we tested Nef:MHC-I BiFC with MHC-I encoding mutations in residues previously implicated in interacting with AP-1 when MHC-I is in complex with Nef (MHC-I Y320 or D327). Co-transfection of plasmids encoding MHC-I Y320A-VN or MHC-I D327N-VN and Nef-VC into HeLa cells revealed a decrease in the BiFC signal relative to wild type MHC-I (Fig. 1a), as measured by Venus mean fluorescence intensity in cells expressing both Nef and Flag tagged MHC-I (Fig. 1b). Similarly, co-expression of Nef-VC and a MHC-I double mutant (MHC-I Y320A/D327N) resulted in a 2-fold reduction in BiFC signal (Fig. 1a,b), indicating that interactions between MHC-I and AP-1 are critical to observe a Nef:MHC-I BiFC signal, supporting the formation of a Nef:MHC-I:AP-1 ternary complex in cells. Importantly, the reductions in BiFC signal with the various MHC-I mutants were not due to differences in protein expression, as revealed by Western blot analysis (Fig. 1c). Furthermore, we demonstrate that the fusion of VN or VC to MHC-I or Nef respectively, did not alter protein function with respect to downregulation, as we previously demonstrated22. Specifically, wildtype MHC-I-VN is downregulated by Nef (Supplemental Fig. S1), unlike the double mutant (Y320A/D327N) (Supplemental Fig. S1). Similarly, Nef-VC is able to efficiently downregulate cell surface MHC-I (Supplemental Fig. S1).
Nef targets recycling MHC-I prior to transit through a Rab11 compartment
An obligate step in cellular homeostasis involves the rapid recycling of MHC-I to and from the cell surface in Rab11-positive recycling endosomes25. We first tested if Nef disrupts this rapid recycling step. To test this, we co-transfected HeLa cells with plasmids encoding MHC-I-eGFP and Rab11a-dsRed (Fig. 2a; panel 2), which labels recycling endosomes26. We then compared this to cells expressing Nef:MHC-I BiFC and Rab11-dsRed, in order to focus specifically on the MHC-I molecules that are targeted by Nef (Fig. 2a: panel 1). Pearson’s correlation analysis revealed that there was ~1.6 fold less co-localization between Rab11-dsRed and Nef:MHC-I BiFC than between Rab11-dsRed and MHC-I-eGFP, suggesting that Nef targets MHC-I prior to MHC-I entering a Rab11 dependent recycling route. Furthermore, an antibody uptake experiment confirmed that the majority of MHC-I that is being targeted by Nef originates from the cell surface (Fig. 2c), as the Nef-MHC-I BiFC signal originating from Nef-VC and MHC-I-VN-Flag strongly co-localized with the BB7.2 antibody (Fig. 2d).
Nef interacts with MHC-I within an early endosomal compartment
Since Nef reroutes cell surface MHC-I away from recycling endosomes (Fig. 2a,b), we next tested if a Nef:MHC-I complex would be located in early endosomes. Early endosomes constitute the initial compartment implicated in the endocytosis and sorting of cell surface cargo15,27. We co-transfected HeLa cells with plasmids encoding Nef-VC, MHC-I-VN-Flag and the early endosomal effector molecule mCherry-Rab5. Fluorescence microscopy analysis revealed that the Nef:MHC-I interaction co-localized with mCherry-Rab5, suggesting that the complex is present in early endosomes (Fig. 3a; Pearson’s = 0.612). Furthermore, in order to confirm that the Nef:MHC-I complex localizes to early endosomes, we co-expressed a constitutively active mCherry-tagged Rab5 molecule (mCherry-Rab5-CA; mCherry-Rab5 Q67L) with Nef-VC and MHC-I-VN-Flag. Expression of Rab5-CA has previously been associated with a build-up of cargo molecules in early endosomes resulting in their subsequent enlargement28. Rab5-CA expression modified the co-localization of the Nef:MHC-I BiFC signal, wherein the BiFC signal was more prominent within Rab5 positive early endosomes (Pearson’s = 0.89), confirming that these vesicles are indeed a transiting point for Nef:MHC-I complexes (Fig. 3a,b). Furthermore, expression of Rab5-CA reduced Nef’s ability to downregulate MHC-I, demonstrating that a functional early endosomal compartment through which Nef-MHC-I complexes can transit is required for optimal downregulation of MHC-I from the cell surface (Supplemental Fig. S2).
Current resolution limits of conventional widefield microscopy do not allow for the visualization of discrete vesicular structures. To determine whether the Nef:MHC-I complex is contained within Rab5 early endosomes, we imaged the Rab5-dependent sorting step using the super-resolution microscopy technique of ground-state depletion microscopy (GSDM). This technique allows for visualization of cellular compartments with a resolution 10X greater than conventional microscopy29. To visualize early endosomes by GSDM, HeLa cells were transfected with the BiFC plasmids (Nef-VC and MHC-I-VN-Flag), and immunostained for Rab5. The 10–25 fold gain in resolution provided by GSDM allowed for the detection of single Nef:MHC-I positive vesicles coated with the early endosomal effector Rab5 (Fig. 3c). Quantification of the association between Nef:MHC-I and Rab5 using the spatial association algorithm (SAA), which calculates the distance between punctate structures of different acquisition channels to determine if they are interacting/co-localizing together30. The SAA analysis revealed that the association of the Nef:MHC-I BiFC complex with Rab5 is significantly greater than that of randomly simulated positions in either acquisition channel (Fig. 3d,e). Taken together, multiple imaging techniques have demonstrated the close localization of the Nef:MHC-I complex with early endosomes suggesting that this organelle plays a key role in the immunoevasive capabilities of HIV-1.
Nef traffics MHC-I to a late endosomal compartment
Early endosomes mature to form late endosomes within the endocytic network14. In order to test if the Nef:MHC-I complex is present in late endosomes we visualized the Nef:MHC-I BiFC signal in the presence of the late endosomal marker Rab7, an effector molecule specifically loaded onto late endosomes31. HeLa cells expressing Nef-VC and MHC-I-VN-Flag were co-transfected with a plasmid encoding mCherry tagged wild type Rab7 (mCherry-Rab7). We observed that the Nef:MHC-I interaction occurs in late endosomes that are positive for mCherry-Rab7 (Fig. 4a). To further control for the Rab7 localization of Nef and MHC-I we utilized dominant-negative mCherry-tagged Rab7 (mCherry-Rab7-DN; Rab7 T22N). Previous expression of Rab7-DN has resulted in defects in trafficking of cargo transiting through late endosomes32. When testing the co-localization of the Nef:MHC-I BiFC with overexpressed mCherry-Rab7-DN, we observed no significant difference in colocalization between the wildtype and dominant-negative Rab7 (Fig. 4a,b). To confirm the late endosomal localization of the Nef:MHC-I complex, we utilized ground-state depletion super-resolution microscopy to gain the resolution needed to identify Rab7-positive vesicles containing the Nef:MHC-I complex (Fig. 4c). We observed that the Nef:MHC-I complex associated closely with Rab7. The SAA analysis revealed that the association of the Nef:MHC-I BiFC complex with Rab7 is significantly greater than that of randomly simulated positions in either acquisition channel (Fig. 4d,e). Taken together, these data demonstrate the localization of the Nef:MHC-I complex within Rab7 positive late endosomes, thereby implicating the late endosome in the re-routing of MHC-I away from the plasma membrane.
Nef and MHC-I are not trafficked to the lysosome, but traffic to the trans-Golgi network
Consistent with the maturation of certain late endosomes to lysosomes27, we next tested if the Nef:MHC-I complex is present in lysosomes. HeLa cells overexpressing Nef-VC and MHC-I-VN-Flag were immunostained with an antibody recognizing LAMP-1, a marker of lysosomes33 (Fig. 5a; panel 1). Immunofluorescence analysis revealed no significant co-localization of the Nef-MHC-I complex with lysosomes (Pearson’s = 0.15), suggesting that the complex does not traffic through this compartment. As lysosomes are a prime site of proteolytic degradation34,35, we sought to control for any degradation of Nef:MHC-I complexes that may occur within lysosomes and thereby mask our ability to detect these complexes within this compartment prior to imaging. Accordingly, HeLa cells were treated with ammonium chloride (NH4Cl) at 20 hours post transfection for 4 hours, which will block lysosomal degradation by increasing the cellular pH and thereby inactivating protease activity within lysosomes (Fig. 5a; panel 2)35. Despite increasing the cellular pH, we were unable to visualize the presence of the Nef:MHC-I complex within LAMP-1 positive lysosomes. In order to confirm that our NH4Cl treatment was sufficient to block lysosomal acidification we treated cells with Lysotracker, an agent retained in acidic compartments or we treated cells with complete media. Fluorescence intensity analysis revealed that Lysotracker dye fluorescence intensity was decreased, and not retained within distinct vesicular structures in the presence of NH4Cl, confirming that our NH4Cl treatment sufficiently inhibits the acidification of lysosomes (Fig. 5c,d).
In order to test the functional consequence of the Nef:MHC-I complex trafficking through early and late endosomes, we designed a lentiviral vector that expressed Flag-tagged MHC-I simultaneously with HIV-1 viral proteins (Fig. 5e). This vector system, which we have previously described22, utilizes the self-cleaving property of the 2 A peptide (F2A) from the foot and mouth virus to express MHC-I that is not linked to HIV-1 Gag/Pol. The infection of SupT1 cells with pNL4–3 F2A-MHC-I-Flag Nef and subsequent Western blotting demonstrated that the presence of Nef does not alter the protein expression levels of MHC-I, confirming that Nef does not direct MHC-I to degradative lysosomal compartment, consistent with previous experiments (Fig. 5e)7,22. Conversely, infection of SupT1 cells with a virus that overexpresses the CD4 receptor, pNL4–3 F2A-CD4-Flag ΔVpu Nef demonstrated that Nef is capable of reducing total cellular CD4 (Fig. 5e), which is consistent with previous reports of Nef-mediated degradation of CD436. Thus, the functional consequence of Nef on MHC-I and CD4 expression is different. In addition, to confirm that Nef expression was not sufficient to traffic MHC-I to lysosomes, we expressed MHC-I-eGFP and Nef-mCherry under non-BiFC conditions and found no significant localization of MHC-I-eGFP with the LAMP-1 lysosomal marker in the presence of Nef, with or without ammonium chloride treatment (Supplemental Fig. S3).
Since the Nef-MHC-I complex did not traffic to lysosomes we sought to identify an alternative subcellular localization for the complex. Previous studies have demonstrated that MHC-I is re-routed by Nef to a paranuclear compartment37. In order to visualize this compartment relative to the Nef:MHC-I complex, we counterstained HeLa cells co-expressing Nef-VC and MHC-I-VN-Flag with a marker of the TGN, TGN4638 (Fig. 6a). A high degree of co-localization was observed between the Nef:MHC-I complex and TGN46 (Fig. 6b; Pearson’s = 0.60) suggesting that the Nef:MHC-I complex traffics to the TGN38. However, our previous work demonstrated that Nef localizes to an uncharacterized Golgi-proximal compartment that cannot be resolved from the TGN in conventional images30,38. As such, GSDM imaging was performed to map the localization of the Nef:MHC-I complexes with high precision. HeLa cells transfected with Nef-VC and MHC-I-VN and immunostained for the TGN marker TGN46 demonstrated the close association of the Nef:MHC-I complex with TGN46 in both the epifluorescence image, and the super-resolution image (Fig. 6c). Quantification of this complex demonstrated a significant increase in the association between the BiFC signal of the Nef:MHC-I complex with TGN46 compared to randomized images, confirming that Nef:MHC-I complexes traffic to the TGN (Fig. 6d,e).
In the present study, we have tracked a complex between the HIV-1 protein Nef and MHC-I within the endosomal network. Our results demonstrate that a Nef:MHC-I complex traffics to both early and late endosomes in addition to the paranuclear TGN compartment. Furthermore, Nef impedes MHC-I from recycling to the cell surface by re-routing MHC-I through both early endosomes and late endosomes and subsequently to the trans-Golgi network. Overall, this mechanism inhibits MHC-I molecules from presenting antigens extracellularly, contributing to the ability of HIV-1 to evade host immune surveillance12.
Nef has the capability to disrupt the normal membrane trafficking events that occur within cells. Indeed, Nef downregulates the cell surface expression of over 36 surface receptors39. Interestingly, there is some degree of specificity within this seemingly non-discriminatory razing of the cell surface topography. This specificity lies within the host membrane adaptor molecules that are used by Nef to internalize specific receptors. For example, the membrane adaptor molecule AP-1 interacts with MHC-I and is implicated in the Nef-dependent internalization of MHC-I8. Conversely, the related membrane adaptor molecule, AP-2, is critical for the Nef-dependent internalization of CD440. Overall, these adaptor molecules are indispensible for the ability of Nef to bind and downregulate MHC-I and CD4 receptors. Structural studies have revealed that the cytoplasmic tails of MHC-I and CD4 mediate this specificity with adaptor molecules. Specifically, AP-1 recognizes the Yxxθ motif (where θ represents a bulky hydrophobic residue) in the MHC-I cytoplasmic tail, as demonstrated by the complex crystal structure between Nef:MHC-I and the mu-1 subunit of AP-110. In contrast, structural modeling of the CD4 cytoplasmic tail has implicated the canonical di-leucine motif of CD4 to be necessary for its downregulation by Nef41. In this study, residues Y320 and D327 in the MHC-I cytoplasmic tail were deemed critical to maintain the Nef:MHC-I complex in cells (Fig. 1). As these residues were implicated in interacting with AP-1 within crystal structures, this supports the formation of a Nef:MHC-1:AP-1 ternary complex within cells, in agreement with previous studies utilizing co-immunoprecipiation8,42. Functionally, the differences in Nef dependent internalization of CD4 and MHC-I are linked to the distinction that AP-2 dependent trafficking is linked to degradative compartments whereas AP-1 dependent trafficking is not7,40,43. We confirmed this assertion as Nef and MHC-I complexes were not directed to degradative compartments (Fig. 5a) and MHC-I was not degraded (Fig. 5e), whereas Nef mediated the degradation of CD4 as previously described (Fig. 5e and ref. 40).
BiFC is a powerful technique that demonstrates the interaction or close proximity of two proteins within a cell. Previously, BiFC was used to illustrate the interaction between Nef and the membrane trafficking regulator PACS proteins, PACS-1 and PACS-2, in late and early endosomes, respectively2. Thus, the BiFC interaction between Nef and MHC-I provides a platform to study the various models of Nef-dependent MHC-I downregulation. Our results demonstrate that newly endocytosed MHC-I originating from the cell surface is targeted by Nef (Fig. 2c,d). Further experiments highlight the presence of the Nef:MHC-I complex throughout the early and late endosomes, and demonstrate the importance of the early endosomal effector molecule Rab5 in this process (Fig. 3 and Supplemental Fig. S2). As Rab5 functions to sort cargo early upon endocytosis44, it is logical that the expression of Rab5-CA, which disrupts the maturation of endosomes44, disrupts Nef-dependent MHC-I cell surface downregulation3. A possible mechanism governing this trafficking step may rely on a ternary complex between Nef, MHC-I and the PACS proteins, as previously demonstrated biochemically for PACS-12. Interestingly, our results are directly linked to the signaling model of Nef-dependent MHC-I downregulation7, which is dependent on the PACS protein dependent endocytosis of MHC-I from the cell surface and not a block of MHC-I trafficking from the endoplasmic reticulum to plasma membrane which is linked to the Nef-dependent degradation of MHC-I in lysosomes45. We failed to observe the localization of the Nef:MHC-I complex in lysosomes even upon rendering these compartments more basic (Fig. 5a–c), suggesting that our experiments closely mimicked early time points in an infection7. This trafficking route would not result in the degradation of MHC-I, a phenomenon observed readily in other viral infections, such as Kaposi-sarcoma related herpesvirus infections, which mediates degradation of MHC-I by the K3 and K5 proteins46. Instead, the Nef:MHC-I complex was routed from Rab5 positive early endosomes to Rab7 positive late endosomes and the TGN and these interactions were observed at the 20 nm resolution offered by GSDM, unequivocally defining the subcellular route undertaken by MHC-I in the presence of Nef (Figs 3, 4 and 6). Moreover, the localization of the Nef-MHC-I complex in Rab7 positive late endosomes is reminiscent of the Nef dependent-localization of SERINC5 within this compartment47,48. SERINC5 was recently identified as a host cellular antiretroviral factor that inhibits HIV-1 replication48. Thus, it appears that Rab7-positive late endosomes are used by Nef to block both infectivity and the CTL response. Overall, Rab7 late endosomes are compartments used by multiple viruses for various steps of the viral infectious cycle including entry49,50 and would also represent a central compartment used by Nef to enable key HIV-1 functions such as the sequestration of MHC-I in an intracellular compartment.
The Nef:MHC-I interaction was also observed in the trans-Golgi network, consistent with the Nef and AP-1 dependent sequestration of MHC-I in a paranuclear compartment (Fig. 6a)37. We postulate that this compartment contains the re-routed MHC-I that has been pulled away by Nef from the cell surface. Previous studies by Blagoveshchenskaya et al. demonstrated that Nef increased the rates of endocytosed MHC-I in an Arf6 dependent manner3. Herein, we demonstrate that this endocytosed MHC-I localizes to the TGN in complex with Nef (Fig. 6). Indeed, we show that MHC-I is excluded from Rab11-positive recycling endosomes when in complex with Nef, suggesting that this represents a membrane trafficking junction point that differs between cells that do or do not express Nef (Fig. 2a,b). In uninfected cells, normal MHC-I recycling occurs via Rab11-positive compartments to enable antigen cross presentation, while maintaining physiological MHC-I levels on the cell surface51,52.
Overall, we propose a model for the Nef-dependent downregulation of cell surface MHC-I that highlights the cellular compartments that are subverted by Nef in order to coordinate the removal of MHC-I away from the cell surface. Our results demonstrate that a Nef:MHC-I complex traffics to both early and late endosomes in addition to the paranuclear TGN compartment. Further studies will be aimed at determining whether the transition between early and late endosomes is required for MHC-I sequestration, or if sequestration of MHC-I is independent of endosomal maturation. This work builds on the model of Nef-mediated MHC-I downregulation by identifying key cellular compartments in which Nef targets MHC-I. Understanding how Nef targets MHC-I will lead to new insights into how viruses mediate immune evasion, and specifically how HIV-1 persists within the infected host.
HeLa and HEK-293T cells (ATCC, Manassas, VA) were grown in complete DMEM (HyClone, Logan, UT) containing 10% fetal bovine serum (FBS) (Wisent, Montreal, Canada) and 100 μg/ml penicillin-streptomycin (HyClone). Sup-T1 cells were grown in complete RPMI (HyClone) containing 10% FBS and 10 μM L-Glutamine (HyClone). All cell lines were grown at 37 °C in the presence of 5% CO2 and sub-cultured in accordance with supplier’s recommendations.
HLA-A2 cDNA (provided by Dr. G. Thomas, University of Pittsburgh Medical School) was subcloned into a pcDNA 3.1 (+) plasmid encoding the N-terminal portion of the Venus fluorophore (VN 1–173), as previously described22. NL4.3 nef was subcloned into a pVC-N1 backbone plasmid encoding the C-terminal portion of the split Venus fluorophore (VC 155–273). HLA-A2 mutants were generated using overlap extension polymerase chain reaction. Expression vectors encoding mCherry-Rab5, mCherry-Rab5-DN, mCherry-Rab5-CA, mCherry-Rab7, mCherry-Rab7-DN, dsRed-Rab11a were provided by Dr. R. Flanagan, UWO and were previously described28,53. The MHC-I-eGFP plasmid was generated by subcloning the HLA-A2 gene into a peGFP-N1 (Clontech) using EcoRI and BamHI restriction digest enzymes. Viral vectors: pNL4.3 F2A-CD4-Flag Δvpu Nef/ΔNef and pNL4.3 F2A MHC-I-MHC-I-Flag Nef/ΔNef were generated by sub cloning MHC-I-Flag and CD4-Flag into the previously described base vector pNL4.3 F2A-X-Nef/ΔNef 22.
For BiFC and subcellular localization studies, 2.5×105 HeLa cells were seeded onto coverslips and 24 hours later plasmids were transfected into cells at equal molar ratios using PolyJet transfection reagent (FroggaBio, Toronto, Canada). Twenty-four hours post transfection cells were incubated for one hour at room temperature to allow for fluorophore maturation, as described previously22. Subsequently, cells were fixed in 4% PFA and prepared for immunofluorescence as described below.
For analysis of BiFC protein expression, HeLa cells were transfected with the specified BiFC vectors, incubated for 24 hours, washed once with phosphate buffered saline (PBS) and lysed in lysis buffer (0.5 M HEPES, 1.25 M NaCl, 1 M MgCl2, 0.25 M EDTA, 0.1% Triton X-100, 1X complete Protease inhibitor Tablets (Roche, Indianapolis, IN)). Cells were incubated on a rotator for 20 minutes at 4 °C before removing insoluble cellular debris by centrifugation at 20,000× g for 20 minutes. Lysates were boiled at 98 °C in 5X SDS-PAGE sample buffer (0.312 M Tris pH 6.8, 25% 2-Mercaptoethanol, 50% glycerol, 10% SDS) and proteins were separated on a 12% SDS-PAGE gel and subsequently transferred to nitrocellulose membranes. Membranes were blocked in 5% non-fat skimmed milk (BioShop Canada, Burlington, Canada) in TBST containing 0.1% Triton X-100 for 1 hour, then incubated overnight at 4 °C with various antibodies: rabbit anti-Nef polyclonal antibody (1:2000; catalog number 2949, NIH AIDS Research and Reference Reagent Program, USA54, rat anti-DYKDDDK monoclonal IgG (1:2500; BioLegend, San Diego, CA), anti-p24 (1:800; catalog number 4121, NIH AIDS Research and Reference Reagent Program, USA) and anti-Actin (1:2000; Thermo Scientific). Membranes were then washed and incubated for two hours with the appropriate species-specific HRP-conjugated antibodies (1:3000; Thermo Scientific). All blots were developed and quantified using ECL substrates (Millipore Inc., Billerica, MA) and a C-DiGit chemiluminescence Western blot scanner (LI-COR Biosciences, Lincoln, NE). To test Nef’s ability to mediate MHC-I or CD4 expression, we utilized our previously described viral vector system22 to express MHC-I-Flag or CD4-Flag, in the presence or absence of Nef. The resulting vectors: pNL4.3 F2A MHC-I-Flag Nef/ΔΝef or pNL4.3 F2A CD4-Flag Δvpu Nef/ΔNef were used to generate pseudoviral particles as previously described22. Sup-T1 cells were subsequently infected, and 48 hours post infection, cells were lysed and levels of MHC-I and CD4 were analyzed by Western blot as described above.
For subcellular localization studies, transfections were performed as described above. Cells used in BiFC studies were incubated at room temperature for 1 hour prior to fixation to allow the reconstituted fluorophore to mature22. All cells were fixed by washing twice with PBS, incubating for 20 minutes in 4% PFA and subsequently washing three times in PBS. To stain for the split fluorophore halves and various intracellular compartments, fixed HeLa cells were incubated in permeabilization/blocking buffer (5% BSA in PBS and 0.2% Triton X-100) for 1 hour. Cells were then incubated with the appropriate antibodies diluted in blocking buffer for 2 hours (anti-Rab5 (Cell Signaling); 1:200, anti-Rab7 (Santa-Cruz); 1:100, anti-LAMP-1 (DSHB); 1:200, anti-TGN46 (Sigma Aldrich); 1:200, anti-Flag (Biolegend): 1:400). Cells were subsequently washed three times in blocking buffer and incubated with the appropriate secondary antibody diluted in blocking buffer (donkey anti-rabbit AlexaFluor 647 or donkey anti-mouse AlexaFluor 647 (1:1000; Jackson ImmunoResearch)) for 2 hours at room temperature. Finally, cells were washed three times in PBS (3 minute each) and mounted onto glass slides using Fluormount-G or DAPI-fluoromount-G (Southern Biotech, Birmingham, AL). Fluorescence intensity of the BiFC signal was measured by selecting cells positive for both Nef and Flag-tagged MHC-I, then subtracting the background fluorescence using ImageJ55,56. To test the localization of MHC-I-eGFP with LAMP-1, cells were transfected with MHC-I-eGFP with Nef-mCherry or mCherry and immunostained as above. Co-localization of MHC-I-eGFP and LAMP-I was then measured in cells expressing either Nef-mCherry or mCherry in the presence or absence of ammonium chloride.
HeLa cells were transfected with equal molar amounts of MHC-I-VN-Flag and Nef-VC. Twenty-four hours post-transfection cells were washed with ice cold PBS, and subsequently anti-HLA-A2 (BB7.2; Biolegend) antibody was added at a dilution of 1:300. Antibody was allowed to bind for 20 minutes at 4 °C. Following antibody binding, cells were washed 3X with cold PBS, and either fixed (time 0 minutes), or supplemented with warm complete media and incubated for 90 minutes at 37 °C. Cells were then fixed, permeablized, and immunostained with donkey anti-mouse AlexaFluor 647 (1:400; Jackson ImmunoResearch) secondary antibody for 2 hours to detect the internalized antibody. Coverslips were then washed 3X in PBS and mounted using DAPI-Fluoromount-G (Southern Biotech).
Ammonium chloride treatment of cells to assess lysosomal trafficking
For assessing lysosomal subcellular localization, staining was performed as described above with anti-human LAMP-1 1:200 (Developmental Studies Hybridoma Bank; Iowa City, IA). Ammonium chloride was added to a concentration of 100 mM to prevent lysosomal acidification for 4 hours. To ensure the ammonium chloride treatment affected lysosomal acidification, HeLa cells treated in an equivalent manner were stained with Lysotracker DeepRed (10 μM; Life Technologies). Live cells were then imaged, and mean fluorescence intensity was measured using ImageJ.
Cells were viewed on a Leica DMI6000 B at 63X or 100X magnification using the FITC, Cy3, CY5 and DAPI filter settings and imaged with a Hamamatsu Photometrics Delta Evolve camera. Images were subsequently deconvolved using the Advanced Fluorescence Deconvolution (Lecia, Wetzlar, Germany) application on the Leica Application Suite software. Co-localization analysis was conducted using Pearson’s Correlation from the ImageJ plugin JACoP, as described previously57.
Super-resolution imaging was performed as previously described30. Briefly, HeLa cells were transfected with plasmid DNA, and 24 hours later immunostained as described above. Prior to imaging, cells were mounted in a depression slide containing 100 mM cysteamine (Sigma) buffer in PBS. Coverslips were sealed with Twinsil (Picodent), and imaged within 4 hours of mounting. All images were acquired using a Lecia SR GSD microscope with the 100X/1.43 NA objective lens containing an additional 1.6X magnifier. Fluorophores were excited using 125 mW–250 mW lasers (488, 532 and 647 nm) and a 30 mW backpumping 405 nm laser. Channels were acquired sequentially at 100 fps for 7,000–15,000 frames, with minor adjustments on laser power and backpumping to maintain 10 to 30 active fluorophores per frame. The resulting images were exported and molecule position files were converted to ASCII for analysis. Intermolecular interactions and localization within labeled endocytic compartments was quantified using spatial association analysis (SAA), performed using our custom-written MIiSR software30, with datasets filtered to remove molecules detected with a precision of <25 nm prior to analysis. All interactions were validated by comparing the observed degree of interaction to that observed in 10 images containing the same number of particles randomly scattered or an image of equal area.
To test the functional significance of disrupting Rab5, HeLa cells were transfected with mCherry-tagged Rab5-CA or Rab5 (wt) constructs in conjunction with Nef-eGFP or empty eGFP encoding backbone. Twenty-four hours post transfection, cells were trypsinized, washed twice with PBS and fixed in 2% PFA for 15 minutes. Following fixation, cells were washed in FACS buffer (0.5% FBS and 50 mM EDTA in PBS) and stained with W6/32 anti-MHC-I (1:4000) antibody for 30 minutes (provided by D. Johnson, Oregon Health and Science University). Cells were then washed twice and stained with donkey anti-mouse AlexaFluor 647 (1:1000) for 20 minutes, followed by two more washes in FACS buffer. Flow cytometry was then performed using a BD FACSCanto (BD Biosciences) and the geometric mean fluorescence intensity of AlexaFluor 647 (MHC-I) was determined for mCherry and eGFP positive cells.
To test the functionality of the MHC-I-VN-Flag fusion proteins, we transfected HeLa cells with vectors encoding MHC-I-VN-Flag or MHC-I-Y320A/D327N-VN-Flag in combination with either Nef-eGFP or eGFP alone. Twenty-four hours post transfection cells, were stained with BB7.2 anti-HLA-A2 (1:4000) and donkey anti-mouse AlexaFluor 647 (1:2000), as above, to specifically detect cell surface MHC-I fusion proteins. Cells were then analyzed by flow cytometry as above and geometric mean fluorescence intensity of AlexaFluor 647 (MHC-I) was determined for eGFP and AlexaFluor 647 positive cells.
To test the functionality of Nef-VC, HeLa cells were transfected with vectors encoding Nef-VC and eGFP as a transfection control, or a vector encoding eGFP alone. GFP positive cells were subsequently analyzed for cell surface MHC-I levels by flow cytometry using W6/32 anti-MHC-I antibody as described above.
How to cite this article: Dirk, B. S. et al. HIV-1 Nef sequesters MHC-I intracellularly by targeting early stages of endocytosis and recycling. Sci. Rep. 6, 37021; doi: 10.1038/srep37021 (2016).
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We acknowledge the NIH AIDS Reagent Program, Drs. Gary Thomas and David Johnson for reagents. This work was supported by operating grants to JDD from the Canadian Institutes of Health Research (CIHR-MOP 286719), the Natural Sciences and Engineering Council (NSERC Discovery grant 435677) and infrastructure grants to JDD from the Canadian Foundation for Innovation. BD is partially supported by an Ontario Graduate Studentship. EP is supported by an Alexander Graham Bell Doctoral Canada Graduate Scholarship from NSERC.
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