Membrane trafficking

Definition

Membrane trafficking is the process by which proteins and other macromolecules are distributed throughout the cell, and released to or internalised from the extracellular space. Membrane trafficking uses membrane-bound vesicles as transport intermediaries. Cargo molecules are enclosed within or associate with the membrane of these vesicles.

Latest Research and Reviews

  • Research | | open

    Integrins are cell-surface adhesion receptors that are modulated by endo-exocytic trafficking, but existing tools to study this process can interfere with function. Here the authors develop β1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlourin tag or a HaloTag to facilitate dye attachment.

    • Clotilde Huet-Calderwood
    • , Felix Rivera-Molina
    • , Daniel V. Iwamoto
    • , Emil B. Kromann
    • , Derek Toomre
    •  & David A. Calderwood
  • Research | | open

    Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.

    • Douglas S. Richardson
    • , Carola Gregor
    • , Franziska R. Winter
    • , Nicolai T. Urban
    • , Steffen J. Sahl
    • , Katrin I. Willig
    •  & Stefan W. Hell
  • Research | | open

    Microtubule organization requires γ-tubulin ring complexes (γTuRCs), but the mechanisms that control γTuRC-mediated microtubule nucleation are unclear. Here the authors show that the DNA polymerase δ catalytic subunit controls noncentrosomal γTuRC activity and regulates the organization of Golgi-derived microtubules.

    • Yuehong Shen
    • , Pengfei Liu
    • , Taolue Jiang
    • , Yu Hu
    • , Franco K. C. Au
    •  & Robert Z. Qi
  • Research | | open

    Microbial pathogens secrete effector proteins into host cells to affect cellular functions. Here, the authors use a yeast-based screen to study around 200 effectors from six bacterial species, showing that over 30% of them interact with the eukaryotic plasma membrane or intracellular organelles.

    • Bethany A. Weigele
    • , Robert C. Orchard
    • , Alyssa Jimenez
    • , Gregory W. Cox
    •  & Neal M. Alto
  • Research |

    McNally et al. identify the retriever complex as required for endosomal cargo recycling. Retriever binds SNX17, the CCC and WASH complexes to govern cell surface expression of integrins, receptors and transporters.

    • Kerrie E. McNally
    • , Rebecca Faulkner
    • , Florian Steinberg
    • , Matthew Gallon
    • , Rajesh Ghai
    • , David Pim
    • , Paul Langton
    • , Neil Pearson
    • , Chris M. Danson
    • , Heike Nägele
    • , Lindsey L. Morris
    • , Amika Singla
    • , Brittany L. Overlee
    • , Kate J. Heesom
    • , Richard Sessions
    • , Lawrence Banks
    • , Brett M. Collins
    • , Imre Berger
    • , Daniel D. Billadeau
    • , Ezra Burstein
    •  & Peter J. Cullen

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