Abstract
Microbial pathogens have evolved several strategies for interacting with host cell components, such as glycosaminoglycans (GAGs). Some microbial proteins involved in host–GAG binding have been described; however, a systematic study on microbial proteome–mammalian GAG interactions has not been conducted. Here, we used Escherichia coli proteome chips to probe four typical mammalian GAGs, heparin, heparan sulphate (HS), chondroitin sulphate B (CSB) and chondroitin sulphate C (CSC) and identified 185 heparin-, 62 HS-, 98 CSB- and 101 CSC-interacting proteins. Bioinformatics analyses revealed the unique functions of heparin- and HS-specific interacting proteins in glycine, serine and threonine metabolism. Among all the GAG-interacting proteins, three were outer membrane proteins (MbhA, YcbS and YmgH). Invasion assays confirmed that mutant E. coli lacking ycbS could not invade the epithelial cells. Introducing plasmid carrying ycbS complemented the invading defects at ycbS lacking E. coli mutant, that can be further improved by overexpressing ycbS. Preblocking epithelial cells with YcbS reduced the percentage of E. coli invasions. Moreover, we observed that whole components of the ycb operon were crucial for invasion. The displacement assay revealed that YcbS binds to the laminin-binding site of heparin and might affect the host extracellular matrix structure by displacing heparin from laminin.
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Introduction
The extracellular matrix (ECM) is a major structure comprising molecules that facilitate cell adhesion, cell-to-cell communication and differentiation among various animal cells1. The ECM components are intracellularly produced by resident cells; once secreted, these molecules deposit and subsequently assemble into the existing matrix2. The ECM is composed of a laminated mesh of fibrous proteins and glycosaminoglycans (GAGs)3,4,5,6. GAGs are carbohydrate polymers that comprise variably sulphated disaccharide repeats of β4GlcUA–α4GlcNAc or β4GlcUA–β3GalNAc. The β4GlcUA–α4GlcNAc repeats contribute to the synthesis of heparin and heparan sulphate (HS), whereas the β4GlcUA–β3GalNAc repeats contribute to the synthesis of chondroitin sulphate B (CSB) and chondroitin sulphate C (CSC)7. GAG chains, including HS, CSB and CSC, are commonly attached to core proteins on the ECM, forming negatively charged proteoglycans. By contrast, heparin is secreted from degranulated mast cells and exists as a free GAG chain, which interacts with the cell surface8. These GAGs exhibit diverse cellular functions according to their composition and polysaccharide chain size7,9.
Several studies have reported that GAGs are involved in cellular structure, recognition, adhesion and signalling4,10,11. In addition, GAGs interact with microbial proteins and these interactions are substantial for microbial pathogenicity6,12. Some pathogens have evolved GAG-binding proteins (GBPs) that recognise host GAGs and use the advantages of these molecules13,14. For example, such binding facilitates bacterial attachment to the host cell surface and in some cases even mediates pathogen dissemination14. Bacterial adhesins are the most common type of bacterial virulence factors and facilitate the adherence of bacterial cells to the host surface. Many fimbrial-type adhesins recognise GAGs and thus mediate attachment to the host as a critical step in the pathogenesis of most infections15.
Some microbial virulence factors involved in host–GAG binding have been described; however, a systematic study on microbial proteome and mammalian GAG interactions has not been reported. Protein microarrays have recently emerged as a useful and powerful tool for conducting high-throughput protein interaction studies. Several proteome microarrays have been fabricated, such as yeast16, Escherichia coli17, Arabidopsis18 and human proteome microarrays19,20. These studies have recently been applied to various research areas, including protein–protein, protein–lipid, protein–DNA, protein–peptide, protein–cell and protein–small molecule interactions21. Here, we conducted a proteome-wide screening of the GAG interactome by using E. coli proteome chips containing approximately 4300 nonredundant proteins. We used four typical mammalian GAGs, namely heparin, HS, CSB and CSC, to profile the interactions of these molecules with the E. coli proteome. Several intracellular and membrane-bound GBPs were identified after thorough screening and bioinformatics analysis. We validated three outer membrane GBPs: MbhA, YcbS and YmgH. YcbS was confirmed as a major molecule for bacterial infection.
Results
Identification of the GAG-interacting proteome by using E. coli proteome chips
We performed proteome-wide screening of the GAG interactome by using E. coli proteome chips. The combined system approaches were used to assess novel GBPs and the key virulence factors were accordingly determined. As shown in Supplementary Fig. S1, we expressed and purified approximately 4300 E. coli proteins to fabricate the E. coli whole proteome chip. These proteins were purified using a high-throughput protein purification protocol and subsequently printed onto chips in duplicate17. We used four typical mammalian GAGs labelled with DyLight 650, heparin, HS, CSB and CSC to profile the interactions of these molecules with the E. coli proteome. The GBPs were identified using a local cutoff of the mean + 2 standard deviations (SDs), which refers to 2 SDs higher than the regional signal mean (protein spot area, 9 × 9) of the specific protein spot. Bioinformatics analyses, namely Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway mapping and motif searches, were performed to determine the biological relevance of the identified GBPs. The outer membrane GBPs were subsequently identified and their functions were validated using E. coli invasion assays involving knockout, complementation, overexpression and blocking strategies. The GAG–GBP interactions were further confirmed using binding affinity (Kd) assays and through flow cytometry. Moreover, the action of GBP hits on GAGs was evaluated using displacement assays.
In the chip assay, we chemically labelled the GAGs with DyLight 650 N-hydroxysuccinimide (NHS) ester (Fig. 1a), which reacted with the primary or secondary amines to generate stable linkages. The DyLight 650-labeled GAGs were individually incubated with the E. coli proteome chip. The representative binding protein signals are shown in Fig. 1b. We used a cutoff value of mean + 2 SDs and identified 185, 62, 98 and 101 proteins as GBPs for heparin, HS, CSB and CSC, respectively (Supplementary Data S1). No binding signal was identified from the negative control image (only DyLight 650 without GAG).
KEGG pathway analysis of the identified GBPs
We used KEGG to delineate the biological relevance of the identified GBPs. Among all the GBPs, two enriched functions (lipopolysaccharide biosynthesis and ribosome) were identified. N-acetylglucosamine (GlcNAc)-harbouring heparin and HS were reported to regulate virulence properties in microbes22; therefore, we further analysed the KEGG function in heparin- and HS-specific GBPs, which bound to heparin or HS but not to CSB or CSC. Overall, 105 heparin- and HS-specific GBPs exist. The results revealed enhanced lipopolysaccharide biosynthesis in heparin- and HS-specific GBPs. In addition, among these GBPs, we identified a unique enriched function governing glycine, serine and threonine metabolism, suggesting that many heparin- and HS-specific GBPs function in bacterial amino acid metabolism. Amino acid metabolism involves virulence factor synthesis in bacteria23; therefore, these findings have further clarified the biological roles of heparin- and HS-specific GBPs in regulating amino acid metabolism, thereby potentially regulating bacterial virulence factor synthesis.
Motif search of heparin- and HS-specific GBPs
We used GLAM2 to identify all the known motifs occurring in a sequence of heparin- and HS-specific GBPs. Specifically, we identified a unique consensus sequence, XPAX(0,1)EA[SV]X(0,1)EXQ[LV]X(0,2)[AR]RLLR, for this GBP group (Supplementary Fig. S2). In addition, the residue 13–18 (X(0,2)[AR]RLLR) of this motif matched five of the six previously reported known binding motifs of heparin24. This motif likely represents the binding site of heparin.
Bioinformatics analyses revealed that the membrane-bound GBPs possibly yielded the most intriguing observation. Because of the cellular location of these molecules, the outer membrane-bound GBPs have a higher likelihood of establishing direct contact with the host-cell GAGs and might thus contribute to bacterial attachment and invasion. Gene ontology of the cellular component of the GBPs by using the chip assays yielded three outer membrane proteins: MbhA, YcbS and YmgH. MbhA binds to heparin, HS and CSB. YcbS and YmgH bind to heparin alone (Fig. 1c). These three GBPs were subsequently used in further analyses to examine the roles of these molecules in host–pathogen interactions.
YcbS is crucial for E. coli invasion into host cells
We investigated whether the binding of the identified outer membrane proteins is critical for the invasion of E. coli into host cells. The three outer membrane proteins were knocked down to investigate the relevance of these molecules in bacterial infections by using invasion assays (Supplementary Fig. S3). Specifically, the bacteria were added to the human ileocecal epithelial cell line HCT-8 for invasion. The extracellular noninvading bacteria were killed after incubating the HCT-8 cells with gentamycin for 1 h25. The cytosol-invading bacteria were subsequently released after disrupting the HCT-8 cells with 0.1% Triton X-100 and quantified through the spread plate method. A wild-type (WT) control was used as a comparison to calculate the percentage of bacterial invasion. As shown in Fig. 2a, only ycbS-lacking E. coli mutants (∆ycbS) showed a significant decrease in bacterial invasion compared with the WT control (p < 0.05). We subsequently transformed the ycbS plasmid (pCA24N–ycbS) to ∆ycbS. The YcbS in ∆ycbS was induced to yield a WT level of expression with 0.0025 mM IPTG. The YcbS can be further induced with higher concentrations of IPTG in a dose-dependent manner. YcbS was nondetectable in ∆ycbS (Fig. 2b). The invasion assay showed that compared with the invasion ability of the WT control, that of ∆ycbS was complemented by the plasmids carrying ycbS at 0.0025 mM IPTG induction (Fig. 2c). Moreover, ycbS induction at higher concentrations of IPTG enhanced the bacterial invasions in ∆ycbS_ycbS (p < 0.05; Fig. 2c). This result indicates that YcbS directly contributed to the capability of E. coli to infect host cells.
Next, we determined whether the outer membrane protein YcbS blocks E. coli invasion into the HCT-8 cells. We preincubated the HCT-8 cells with different concentrations (0, 50 and 100 nM) of purified YcbS, followed by assessment by using invasion assays. The AroP protein was used as the negative control because it is not in our GBP list. The results revealed that blocking with YcbS gradually reduced the percentage of successful E. coli invasion into the host cells compared with the WT control cells (p < 0.05; Fig. 2d). The negative control cells exhibited no blocking effects. This result further confirms that YcbS facilitates E. coli invasion.
The ycb operon plays a critical role in E. coli invasion into host cells
YcbS is a component of a putative chaperone-usher fimbrial operon in E. coli26. Therefore, we investigated whether the other components of this operon also contribute to E. coli–host cell interactions. We performed invasion assays in different E. coli mutants lacking ycbQ, ycbR, ycbS, ycbT, ycbU, ycbV, or ycbF. To ensure the invasion assay result was solely because of the invasion ability, not the growth ability in the assay period, the ycb-lacking E. coli mutants were examined to ensure that they exhibited equivalent growth curves with the WT control (Supplementary Fig. S4). Our results showed that all the mutants, except ycbF, had a lower invasion ability than the WT control did (p < 0.05; Fig. 3), indicating that the ycb operon plays a major role in bacterial invasion.
Binding affinity between YcbS and heparin
YcbS showed a strong binding affinity for heparin (Fig. 1b); therefore, we further measured the Kd of YcbS to heparin by using surface plasmon resonance (SPR). Purified YcbS was first immobilised onto the SPR sensor chip. Different concentrations of heparin (0.8, 4, 20 and 100 μM) were subsequently used to probe with the YcbS chip. The result revealed that YcbS was bound to heparin with a Kd of 6.9 μM (Fig. 4a).
We then investigated whether YcbS binds to the heparinised surfaces of mammalian cells through flow cytometry. HCT-8 cells were also used as a host cell model. A DyLight 650-labeled anti-Histidine (His) antibody was used to stain YcbS. Moreover incubation with the anti-His antibody alone was considered the negative control. DyLight 650 signals on cells incubated with YcbS were significantly higher (p < 0.05; Fig. 4b), thus confirming that YcbS binds to HCT-8 cells.
YcbS displaces heparin–laminin binding
Laminins bind to heparin in laminin globular (LG) domains, which play an crucial role in regulating the ECM assembly and stabilise the cell membrane structure27. Moreover, the heparin-binding domain of LG domains is a bacterial target. Interference with the laminin–heparin interaction by high-affinity heparin-binding pathogens might destabilise the cell membrane structure, possibly resulting in the loss of appropriate signals and a significant disruption of the cell function28,29. The present findings revealed YcbS–heparin interactions, which involve bacterial invasions; therefore, we hypothesised that YcbS targets the heparin–laminin interaction. To test this hypothesis, we performed displacement assays to characterise YcbS-mediated heparin–laminin binding through a chip assay approach. Protein chips containing printed laminin were preincubated with DyLight 650-labeled heparin for conducting the binding reaction. After several washes, the displacement assay was performed through further incubation of the chips with different concentrations of purified YcbS (Fig. 5a). The results revealed that YcbS dose-dependently reduces the fluorescence signals of DyLight 650-labeled heparin bound to laminin (Fig. 5b,c). Bovine serum albumin (BSA) was used as a negative control. This result indicated that YcbS targets heparin–laminin interactions by displacing heparin from binding to laminin. This indicated that YcbS binds to the laminin-binding site of heparin and potentially disrupts the host ECM structure, suggesting that YcbS is crucial for bacterial invasion.
Discussion
We conducted a systematic proteome-wide screening of the E. coli protein interactome of four typical mammalian GAGs. Hundreds of proteins were identified as GBPs. We used gene ontology to visualise the enrichment of GBPs in cellular component, biological process and molecular function. We observed many terms related to cytoplasm were enriched in cellular component (Supplementary Fig. S5a). Analysis in biological processes further revealed several enrichments regarding metabolism (Supplementary Fig. S5b). This result indicated that most of the GBPs were located intracellularly and has functions involving bacterial metabolism, which might reflect interactions of GBPs that contribute to the biosynthesis of the bacterial capsule or other bacterial virulent components with a composition similar to that of mammalian GAGs30,31. In addition, the molecular function of GBP revealed nucleic acid-binding proteins as the most dominant protein class (Supplementary Fig. S5c). This result was expected because many nucleic acid-binding proteins tend to bind to negatively charged molecules, such as DNA and RNA and sulphated GAGs32. Among the four GAGs, heparin bound to most identified GAG hits, reflecting that heparin is the most negatively charged biological molecule that has been identified33,34,35. As previously described, heparin provides the perfect surface for the attachment of both Gram-positive and -negative bacteria through binding to positively charged proteins on the bacterial surface36.
In this study, we focused on three outer membrane GBPs to investigate the implications of these molecules on E. coli adherence to the host cells. Invasion assays revealed that YcbS not only binds to the surface of mammalian cells but also plays a crucial role in the invasion of host cells. We also observed that not only ycbS but also nearly all the components of the ycb operon (ycbQ, ycbR, ycbS, ycbT, ycbU and ycbV) are critical for bacterial invasion.
ycb is a cryptic operon system in E. coli K12, producing an extracellular fimbrial-like structure that is crucial for forming biofilms26. Therefore, the regulation of this fimbrial-like structure by the ycb operon likely contributes to the bacterial attachment to the host cell surface. YcbQ, another component of the ycb operon, also binds to laminin and plays a major role in bacterial adherence to the host cells37. Therefore, the results revealing YcbS–heparin binding extend the current understanding of the functional roles of the ycb operon in E. coli infection. In addition, YcbQ and YcbS are two outer membrane components of the fimbrial structure produced by the ycb operon38, suggesting that these two proteins act as bacterial adhesins and establish a direct interaction with the host cell surface. Considering the discrepancy in the binding preference of YcbS and YcbQ to host ECM components, whether these two proteins function synergistically or alternatively bind to diverse host cell surfaces should be determined. The displacement assay revealed that YcbS displaces heparin from binding to laminin, indicating that YcbS probably contributes to bacterial infection by disrupting the heparin–laminin binding on the host cellular surface. Notably, the heparin–laminin interaction is a pivotal aspect of mammalian ECM organisation, contributing to a series of interactions that result in tight adherence, subsequent internalisation and potential downstream inflammatory responses that are characteristic of infections39. Therefore, YcbS and YcbQ might act cooperatively to facilitate bacterial infections.
In addition to the finding of a novel role of YcbS, our chip assay revealed hundreds of proteins interacted with GAGs. Despite their biological relevance owing to GAG binding, the potential use of GBPs is crucial for the development of disease therapeutics, not only aimed at infection-related diseases but also at cancer treatment. For example, GBPs would be useful for developing biologically active GAG-binding peptides, thus demonstrating an alternative drug therapy against cancers40.
In conclusion, this is the first study to systematically screen four typical mammalian GAGs, providing a list of GBPs that are potentially crucial for both further molecular studies and the development of novel therapeutic treatments targeting interactions with cellular GAGs. Further analyses of the outer membrane proteins revealed a role of the ycb operon in E. coli invasion.
Methods
Bacterial strains, cell culture and chemicals
Single gene knockout mutants of E. coli K12 were obtained from the Keio collection41; these mutants were designed to create in-frame deletions upon the elimination of the resistance cassette. The open reading frame (ORF) clones of E. coli K12 were obtained from the pCA24N-based overexpression ASKA library42. Each clone encodes a protein of a predicted ORF attached to His under the control of an IPTG-inducible promoter. Kanamycin (50 μg/mL) was used for preculturing the Keio knockout mutants. The ASKA ORF clone was maintained by adding chloramphenicol (30 μg/mL).
The human ileocecal epithelial cell line HCT-8 (American Type Culture Collection, CCL-244) was maintained in a complete medium composed of an RPMI-1640 medium (Sigma–Aldrich) supplemented with 1 mM sodium pyruvate (Sigma–Aldrich) and 10% (v/v) heat-inactivated horse serum (Invitrogen) at 37 °C under 5% CO2 and 95% humidity. The cells were subcultured twice a week at a ratio of 1:3 to 1:8.
The GAGs (Sigma–Aldrich; range, 10–15 KDa) were heparin, HS, CSB and CSC and these molecules were used for proteome microarray screening.
Fabrication of the E. coli proteome chips
We constructed a proteome chip comprising approximately 4300 individual purified full-length E. coli proteins, as previously reported12. E. coli clones harbouring vectors expressing 6× His-tagged proteins42 were inoculated in 96-deep well plates (Nunc). Expression was subsequently induced using IPTG for 3.5 h; the clones were pelleted and stored at −80 °C overnight.
For protein purification, the thawed cell pellets were resuspended in a lysis buffer composed of 50 mM NaH2PO4, 300 mM NaCl, 30 mM imidazole, CelLyticB, 1 mg/mL lysozyme, 50 units/mL benzonase, a proteinase inhibitor mixture, 1 mM phenylmethanesulfonyl fluoride and Ni-NTA Superflow resins (Qiagen). Following a 2.5-h incubation on a plate shaker at 4 °C, the mixtures were transferred into 96-well filter plates (Nunc) and subsequently washed with wash buffer I (50 mM NaH2PO4, 300 mM NaCl, 20% glycerol, 20 mM imidazole and 0.1% Tween 20, pH 8.0) and wash buffer II (50 mM NaH2PO4, 150 mM NaCl, 30% glycerol, 30 mM imidazole and 0.1% Tween 20, pH 8.0) and subsequently eluted with an elution buffer (50 mM NaH2PO4, 150 mM NaCl, 30% glycerol, 300 mM imidazole and 0.1% Tween 20, pH 7.5). The purified proteins were printed in duplicate on Biao aldehyde-coated slides by using ChipWriter Pro (Bio-Rad) and stored at −80 °C until further use.
DyLight 650 labelling of GAGs
The GAGs were chemically labeled with DyLight 650 (Thermo Fisher Scientific). A mixture of 0.5 mM of each GAG was dissolved in 50 mM borate buffer and 10 mg/mL of a DyLight 650 NHS ester (molar ratio, 1:20) was incubated at 4 °C for 2 h. The reaction mixture was subsequently incubated with a 10-fold molar excess of Tris-HCl buffer (pH 8.5) to quench the unreacted NHS group and stored at −80 °C until further use.
Probing the E. coli proteome chips with GAGs
Each E. coli proteome chip was blocked with 1% BSA to reduce nonspecific binding. Approximately 0.5 μM DyLight 650-labeled heparin, HS, CSB and CSC in Tris-buffered saline (TBS) with 0.05% Tween 20 and 1% BSA were individually probed with the chip at room temperature for 1 h. Following incubation, the chips were sequentially washed with TBS with 0.05% Tween 20 and distilled water for 5 min to remove excess unbound GAGs. The chips were dried through centrifugation at 201 × g and subsequently scanned using a LuxScan-10 K/A array scanner (CapitalBio). The binding signals were acquired and analysed using GenePix Pro 6.0 software. Each GAG-binding experiment was conducted in three separate E. coli proteome chips.
Bioinformatics analysis
GBPs were selected on the basis of a local cutoff, defined as 2 SDs above the regional signal mean (protein spot area, 9 × 9) of the specific protein spot. ProCAT43 was used for signal normalisation. All identified GBPs were mapped into functional groups by using the KEGG database44,45. The consensus motif was searched using GLAM2. The identified GBP sequences were converted to the FASTA format and analysed using GLAM2 to survey consensus motifs46. The GLAM2 parameters were set as the default and the resulting motifs were searched through the entire E. coli proteome by using GLAM2SCAN.
Quantitative real-time reverse transcription PCR (QPCR)
The total cellular RNA was extracted using the MagNA Pure Compact System (Roche). The quantity of RNA was determined using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). RNA samples were reverse-transcribed for 120 min at 37 °C with the High Capacity cDNA Reverse Transcription Kit according to the manufacturer’s instructions (Applied Biosystems). QPCR was performed using the 2 × Power SYBR Green PCR Master Mix (Applied Biosystems) and 200 nM forward and reverse primers under the following conditions: 10 min at 95 °C, 40 cycles of 15 s at 95 °C and 1 min at 60 °C. The used primer sequences are as follows: forward 5′-GAACAGCACCGGGCTGAA-3′ and reverse 5′-TAACGACGCCGCATCAAGT-3′ for ycbS and forward 5′-ATACCGCATAACGTCGCAAGA-3′ and reverse 5′-GTGAGCCGTTACCCCACCTA-3′ for 16S rRNA. Each assay was run in triplicate on an Applied Biosystems 7300 Real-Time PCR system (Applied Biosystems) and expression fold changes were derived using the comparative CT method. Moreover, 16S rRNA was used as the reference gene to normalise specific gene expression in each sample.
Complementation of ycbS in the Keio E. coli mutants
The ycbS-lacking E. coli mutant was obtained from the Keio collection41. To complement the Keio ycbS mutants, the plasmid pCA24N–ycbS harbouring ycbS was obtained from the ASKA clone library42 and used to transform the ycbS mutant, as previously described47.
Quantitative cell invasion assays
To determine the bacterial invasion into epithelial cells, the spread plate bacterial colonies were first preinoculated in 3 mL of a fresh Luria–Bertani (LB) medium with shaking overnight at 37 °C and 30 × g. Approximately 1 mL of the bacterial cultures were then inoculated into 20 mL of a LB medium and incubated with shaking at 37 °C and 30 × g until they reached the midlogarithmic phase (optical density at 600, 1.0). For the cell invasion assays, 2 × 107 CFU/mL of bacterial cells were added to confluent HCT-8 cells (approximately 2.3 × 105 cells in a 12-well tissue culture plate) in a complete medium composed of an RPMI-1640 medium supplemented with 1 mM sodium pyruvate and 10% heat-inactivated horse serum. The plate was incubated at 37 °C under 5% CO2 and 95% humidity for 3 h. The infected epithelial cells were subsequently incubated with 100 μg/mL of gentamycin in a complete medium after five washes with an RPMI-1640 medium (Sigma–Aldrich). Furthermore, the cells were incubated for an additional 1 h at 37 °C under 5% CO2 and 95% humidity to kill the extracellular bacteria, as previously reported25. Next, the HCT-8 cells were washed five times with an RPMI-1640 medium and disrupted with 0.1% Triton X-100 and the released bacterial cells were quantified through the spread plate method. The cell invasion efficiency was determined as the percentage of bacterial cells recovered from the five biological repeat wells compared with 100% efficiency in the cells infected with their isogenic parental strain (WT control).
In experiments in which ycbS was supplied using the pCA24N plasmid, the spread plate bacterial colonies were first preinoculated in 3 mL of a fresh LB medium with shaking overnight at 37 °C and 30 × g. Approximately 1 mL of the bacterial cultures were subsequently transferred to 20 mL of a fresh LB medium and incubated with shaking at 37 °C and 30 × g until they reached an optical density at 600 of 0.7. Next, different concentrations of IPTG was added to the bacterial cultures, including the WT control and the cultures were incubated at 37 °C and 30 × g for 3 h48 to ensure YcbS expression from the inoculated bacteria during the invasion assay.
Blocking assays
The purified YcbS (10, 50 and 100 nM) was incubated with the HCT-8 cells at 37 °C for 1 h. The cells were subsequently washed twice with an RPMI-1640 medium (Sigma–Aldrich) and subjected to cell invasion assays, as described in the previous section.
Kd determination by using SPR
A customised SPR biosensor49 built according to wavelength interrogation with a five-channel Teflon flow cell was used to monitor the recognition interaction between heparin and YcbS. The bare gold surface of an SPR chip was first thoroughly washed with deionised water and absolute ethanol, dried under argon and immediately immersed in a mixture of 0.5 mM 11-amino-1-undecanethiol hydrochloride and 1.5 mM 6-mercapto-1-hexanol in absolute ethanol. The chip surface was subsequently immersed in 12.5% glutaraldehyde for 1 h at room temperature. Furthermore, YcbS (1 μM) was covalently immobilised on the activated surface overnight; the sensor chip surface was cleaned with pure water and dried using nitrogen before use. To obtain interaction kinetic measurements, the SPR was stabilised in TBS for 15 min. The indicated concentration of heparin was injected for 15 min (flow rate = 18.2 μL/min) and subsequently dissociated for 15 min. The affinity interactions between immobilised YcbS and heparin in the solution were determined, as previously reported50.
Flow cytometry
Flow cytometry was used to validate YcbS binding to the HCT-8 cells. YcbS was purified and verified through sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). The HCT-8 cells were detached using 10 mM ethylenediaminetetraacetic acid–phosphate-buffered saline (PBS) and incubated with 100 nM YcbS at 4 °C for 1 h. The cells were subsequently washed twice with PBS containing 0.1% BSA at 157 × g for 5 min to remove the unbound proteins. The cells were further stained with DyLight 650-tagged anti-His antibody at 4 °C for 30 min to capture the cell–YcbS complex. After washing through centrifugation at 157 × g for 5 min, 5000 cells were collected from each sample and analysed using the Muse Cell Analyser (Merck Millipore). The processed data were acquired as screenshots and the text size was edited for clarity. All the results were compared with untreated cells stained with anti-His antibody in triplicate.
YcbS-induced displacement of heparin–laminin binding
To evaluate YcbS displacement in heparin–laminin binding, the protein chips were printed using 150 μg/mL of laminin (EMD Millipore) and immobilised at 4 °C for 12 h. The chips were subsequently incubated with 1.34 μM of DyLight 650-labeled heparin for 1 h at room temperature to facilitate laminin binding to DyLight 650-labeled heparin. After being washed with TBS containing 0.05% Tween 20, the chips were further incubated with three concentrations (4, 20 and 100 nM) of purified YcbS and rinsed with distilled water following incubation for 20 min. The remaining DyLight 650-labeled heparin signal was obtained using a LuxScan laser scanner (CapitalBio).
Statistical analyses
The cumulative hypergeometric distribution (or Fisher exact test, referred to as the hypergeometric test) was used to score the motif enrichment. Invasion assay results were presented as mean ± standard error; the experimental and control groups were compared through the two-tailed Student t test.
Additional Information
How to cite this article: Hsiao, F. S.-H. et al. Systematic protein interactome analysis of glycosaminoglycans revealed YcbS as a novel bacterial virulence factor. Sci. Rep. 6, 28425; doi: 10.1038/srep28425 (2016).
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Acknowledgements
We thank Dr. Wen-Yih Chen, Mr. Chung-En Lin and Yi-Ju Chen for their assistance with SPR. This study was supported by the Ministry of Science and Technology, Taiwan (MOST 104-2320-B-008-002-MY3 and MOST 104-2627-M-008-001), Aim for the Top University Project (104G603-6), Cathay General Hospital (104CGH-NCU-A1) and Landseed Hospital (NCU-LSH-104-A-001).
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F.S.-H.H. and C.-S.C. designed the experiments. C.-S.C. supervised the project and F.R.S. fabricated the proteome chip. F.S.-H.H. performed GAG labelling and chip assays. F.S.-H.H. and J.-M.L. screened all the GBPs. Moreover, F.R.S., G.-D.S. and J.-M.L. performed the bioinformatics analysis. F.S.-H.H. and G.-D.S. performed the flow cytometric assays. F.S.-H.H. and Y.-W.C. performed all the invasion assays; F.S.-H.H. performed the binding affinity and displacement assays. F.S.-H.H., F.R.S. and C.-S.C. drafted and revised the manuscript.
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Hsiao, FH., Sutandy, F., Syu, GD. et al. Systematic protein interactome analysis of glycosaminoglycans revealed YcbS as a novel bacterial virulence factor. Sci Rep 6, 28425 (2016). https://doi.org/10.1038/srep28425
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DOI: https://doi.org/10.1038/srep28425
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