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Gamma oscillations in V1 are correlated with GABAA receptor density: A multi-modal MEG and Flumazenil-PET study

  • Scientific Reports 5, Article number: 16347 (2015)
  • doi:10.1038/srep16347
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Abstract

High-frequency oscillations in the gamma-band reflect rhythmic synchronization of spike timing in active neural networks. The modulation of gamma oscillations is a widely established mechanism in a variety of neurobiological processes, yet its neurochemical basis is not fully understood. Modeling, in-vitro and in-vivo animal studies suggest that gamma oscillation properties depend on GABAergic inhibition. In humans, search for evidence linking total GABA concentration to gamma oscillations has led to promising -but also to partly diverging- observations. Here, we provide the first evidence of a direct relationship between the density of GABAA receptors and gamma oscillatory gamma responses in human primary visual cortex (V1). By combining Flumazenil-PET (to measure resting-levels of GABAA receptor density) and MEG (to measure visually-induced gamma oscillations), we found that GABAA receptor densities correlated positively with the frequency and negatively with amplitude of visually-induced gamma oscillations in V1. Our findings demonstrate that gamma-band response profiles of primary visual cortex across healthy individuals are shaped by GABAA-receptor-mediated inhibitory neurotransmission. These results bridge the gap with in-vitro and animal studies and may have future clinical implications given that altered GABAergic function, including dysregulation of GABAA receptors, has been related to psychiatric disorders including schizophrenia and depression.

Introduction

Neuronal oscillations across multiple frequencies are a prominent feature of brain function. In particular, high-frequency neural oscillations and broadband activity in the gamma-band (ca. 30–200 Hz) have been demonstrated to be involved in a wide range of primary and high-level cognitive processes1,2,3,4,5,6,7,8,9,10,11. Interestingly, the blood oxygen level dependent signal (BOLD) used in functional magnetic resonance imaging has also been shown to correlate with the gamma-band activity12, across a variety of brain regions13,14.

Yet, despite being the subject of a thriving field of research, the neurochemical mechanisms underlying the emergence of gamma-band oscillations in the human brain remain poorly understood. Evidence from animal studies suggests that they are generated by a neuronal network containing interconnected pyramidal cells and GABA (gamma-aminobutyric acid)-ergic inhibitory interneurons15,16. In line with this, gamma-oscillations measured with Magnetoencephalography (MEG) have been shown to depend on GABA concentration levels estimated via magnetic resonance spectroscopy (MRS) in primary visual17,18, primary motor19, left dorsolateral prefrontal cortex20. However, MRS-based GABA-Gamma correlations are challenged, to a certain extent, by partly inconsistent findings21,22,23, as well as failure to replicate the correlation between GABA-concentration and gamma oscillation frequencies in V124. These diverging results could be due to the fact that MRS GABA concentration does not specifically quantify synaptic GABA and that it is susceptible to macromolecule contamination24. Other more specific markers of GABAergic synaptic signaling might thus be necessary to reliably probe its putative link to gamma oscillations.

We hypothesized that GABAA receptor density, which is more closely related to inhibitory neurotransmission than total GABA concentration, could provide a more direct assessment of the relationship between GABAergic signaling and gamma oscillations in humans. Recent findings in rodents have shown that high-frequency oscillations in the ripple range (80–250) are abolished following focal GABAA receptor blockade25. Furthermore, in a mouse model of schizophrenia, the disruption of GABAA receptor subunit clustering has been associated with a reduction of gamma power in the ca. 40–70 Hz range26. In addition, ample evidence from modeling and in-vitro studies point to the key role of GABAA receptor mediated inhibition in the generation of gamma oscillations8,16,27,28. Reliable evidence for such a link in humans is still missing.

We set out to evaluate the effects of variations in GABAA receptor distribution, estimated during rest, on rhythmic activity recorded during visual perception. To this end, we investigated the relationships between the timing, amplitude and frequency of alpha-, beta- and gamma-band oscillatory activity (determined with MEG) and GABAA receptor density (determined with 11C-flumazenil positron emission tomography, FMZ-PET) in primary visual cortex (V1). We hypothesized that the PET-based GABAA receptor measure would form an informative proxy for the strength of inhibition in the V1 neural network, and, thus, predict the properties of visual gamma-band oscillations across individuals. Our results demonstrate that the density of GABAA receptors correlates positively with gamma peak frequency and negatively with gamma amplitude in human primary visual cortex.

Results

To investigate the relationship between GABAergic inhibition and high-frequency oscillations in V1, we measured GABAA receptor density using FMZ-PET and gamma oscillation dynamics with MEG in the same population of healthy individuals (n = 13, age 19–29 yrs, mean 24 yrs). The FMZ-PET data used to determine the GABAA receptor density were collected while the subjects were resting in the scanner, and the characteristics of oscillatory neuromagnetic signals in each individual were estimated from their visual responses in a standard n-back working memory task.

Significant modulation of alpha-, beta and gamma-band activity and high density of GABAA receptors in V1

Visual stimulation at task onset induced a significant (p < 0.005) prominent gamma-band response within a window of 0–600 ms in bilateral occipital and occipito-temporal cortices (Fig. 1a). In parallel, both alpha and beta bands showed significant (p < 0.005) suppression of activity with respect to baseline (-200 to 0 ms) in medial occipital cortex (Fig. 1a). No significant modulation of activity was detected in the theta-band (4-7 Hz) at the selected threshold (p < 0.005). The highest density of GABAA receptors across the cortex was evident in the bilateral medial occipital cortex (Fig. 1b). The observed MEG alpha- and beta-band suppression, the gamma-band increase and the peak of the GABAA receptor density in the occipital cortex all fell within or showed spatial overlap with the anatomically defined primary visual cortex (Fig. 1c). In addition to the visual regions, there was both modulation of gamma-band activity and a high density of receptors in the left cingulate cortex. In the bilateral V1, the stimulation induced a prominent gamma-band response at around 100–500 ms and in almost the entire evaluated frequency range, peaking at ~300 ms and ~65 Hz (Fig. 1d). The alpha- and beta-band suppressions occurred slightly later, peaking at around 400 ms and 12 Hz (alpha) and at ~350 ms and ~20 Hz (beta). The responses showed substantial variability across subjects, particularly for the gamma-band peak frequency and timing (Fig. 2).

Figure 1: Medial views of modulation of neural activity, distribution of GABAA receptors and the primary visual cortex (V1).
Figure 1

(a) Modulation of neural activity in the alpha-, beta- and gamma-bands with respect to baseline time-window (paired t-test, p < 0.005, uncorrected). (b) Cortical GABAA receptor density across subjects (threshold at mean + 1 SD). (c) Consistency of V1 in the template brain across subjects. (d) Group-level neural responses (normalized units) in the anatomically defined bilateral V1. The data are shown for the 10 subjects for whom both MEG and PET data were recorded successfully. Cortical-level visualization was performed using FreeSurfer software57.

Figure 2: Anatomical definition of and the neural responses in V1 for 2 subjects.
Figure 2

Anatomically defined V1 (top), time-frequency representations (TFR) of the neural responses (middle), and the time-courses of modulation of activity in the alpha-, beta, and gamma-bands (bottom). In the TFRs, the black lines show the spectral behavior across the 0 to 600 ms time-window. In the lower bands (5–30 Hz), the curves have been inverted (prominent deflection indicates strong suppression). Cortical-level visualization was performed using FreeSurfer software57.

GABAA receptor density correlates with gamma-band frequency and amplitude in V1

The correlation analysis between FMZ-PET data and MEG neural response properties in V1 revealed a significantly positive correlation between GABAA receptor density and gamma peak frequency (rho = 0.74, p = 0.014; 95% confidence limits for correlation, CI95%, 0.50 − 0.90; Fig. 3a) and a significantly negative correlation between GABAA receptor density and gamma amplitude (rho = -0.70, p = 0.031; CI95% = −0.86 −0.45; Fig. 3b). The amplitude and frequency of the gamma-band activity were, however, not mutually correlated (rho = -0.47, p = 0.17). By contrast to the gamma-band, the alpha- and beta-bands did not show significant correlations with GABAA receptor density, neither for peak amplitude (alpha, rho = -0.07, p = 0.86; CI95% = −0.44 - 0.33; beta, rho = -0.02, p = 0.97; CI95% = −0.50–0.42) nor for frequency (alpha, rho = -0.20, p = 0.58; CI95% = −0.71 − 0.06; beta, rho = 0.51, p = 0.14; CI95% = 0.27 − 0.80; Fig. 3). Moreover, no significant correlation between peak timing and GABAA receptor density was detected in any of the frequency bands (alpha, rho = 0.23, p = 0.53; beta, rho = 0.6, p = 0.073; gamma, rho = 0.40, p = 0.25).

Figure 3: MEG-PET correlation in V1.
Figure 3

Correlation between the total GABAA receptor density and the modulation peak (a) frequency and (b) amplitude in the gamma-, alpha-, and beta-bands.

Correlation between GABAA receptor density and gamma-band activity is a specific mechanism in V1

To assess the robustness and specificity of the correlation observed between GABAA receptor density and V1 gamma oscillation properties, we conducted extensive additional analyses exploring multiple potential confounds. First, to make sure that the observed correlations were specifically linked to the GABAergic properties of the V1 region, we recomputed the correlations replacing V1 receptor density with the overall brain-wide GABAA-density. The results (Fig. 4a) demonstrate that the amplitude and frequency properties of V1 gamma-band activity are not correlated with the total GABAA-density (amplitude, rho = -0.33, p = 0.35; CI95% = −0.64 − 0.00; frequency, rho = 0.44, p = 0.21; CI95% = 0.13 − 0.71). We also tested whether correlations could arise between the gamma-activity properties and anatomical properties of V1: No significant correlation was detected between the gamma properties and volume (amplitude, rho = -0.14, p = 0.71; frequency, rho = 0.57, p = 0.084 CI95% = 0.32 − 0.74) or surface area (amplitude, rho = -0.05, p = 0.89; frequency, rho = 0.57, p = 0.084; CI95% = 0.20 − 0.71) of V1 (Fig. 4bc). Furthermore, given that previous reports have reported relationships between GABAA receptor and neuronal density29 and between gamma frequency and cortical surface area30, we also tested whether the observed correlations subsist after regressing out the effect of these parameters. To this end, we replicated the main correlation analyses using PET data from which we first regressed out V1 gray matter density and surface area. The conducted regression strengthened the correlation measured between GABAA receptor density and gamma-band peak frequency (rho = 0.86, p = 0.0013; CI95% = 0.73 − 0.98), whereas the correlation between the receptor density and gamma-band amplitude only showed a trend (rho = -0.56, p = 0.096; CI95% = −0.83 −0.14). The correlations results summarized in Table 1 also show that this tendency was further confirmed using a Bayes factor (BF) analysis, which provided evidence against the null hypothesis of no correlation with gamma frequency (BF = 6.0064; Pearson’s r = 0.75973, p = 0.0108) but no evidence against the null hypothesis when it came to gamma-amplitude (BF = 1.4159, Pearson’s r = -0.61411, p = 0.0589).

Figure 4: Correlation between V1 gamma-band activity, whole-brain GABAA receptor density and size of V1.
Figure 4

(a) Correlation between the whole-brain receptor density and the peak frequency and amplitude. (b) Correlation between the volume of V1 and the peak frequency and amplitude. (c) Correlation between the surface area of V1 and the peak frequency and amplitude.

Table 1: Correlation between GABAA receptor density and gamma-band activity in V1.

To further probe the robustness of our findings, we also computed correlations between V1 gamma oscillation characteristics (peak frequency and amplitude) and the PET-based GABAA receptor density in randomly selected sets of voxels across the brain (either randomly distributed voxels or spatially contiguous random voxel sets). The magnitude of the correlations between the V1 MEG estimates and PET GABAA receptor data from any of the 200 random sets of PET-voxels was systematically lower than the ones obtained with the corresponding V1-PET values. We also explored whether our findings depend on the way the gamma-band modulations were evaluated. By replacing absolute with percent signal change, a significant correlation was still present between the GABAA receptor density and the peak gamma frequency (rho = 0.84, p = 0.0024; CI95% = 0.68 − 0.97); however, the correlation between GABAA receptor density and peak amplitude was no longer significant (rho = -0.34, p = 0.36; CI95% = −0.57 − 0.12). Finally, we found that neither peak frequency nor amplitude of V1 gamma correlated with the subjects’ age (frequency, rho = 0.47, p = 0.17; amplitude, rho = -0.10, p = 0.77).

Discussion

By combining MEG and Flumazenil-PET measurements the current study reveals that the density of GABAA receptors correlates positively with gamma peak frequency and negatively with gamma amplitude in primary visual cortex. These results support the view that gamma-range neuronal response profiles across individuals are shaped by inhibitory neurotransmission, assessed by GABAA receptor density.

The prominent correlation between GABAA receptors and gamma peak frequency is in-line with modeling work31 which predicts that the frequency strongly depends on the balance between AMPA and GABA synaptic currents. This may be explained by the fact that both pyramidal-to-interneuron connections and the pyramidal-to-pyramidal connections tend to decrease population frequency. Our findings thus improve our understanding of the neurochemical mechanisms that determine gamma oscillations in human cortex. Such insights are critical given the importance of precise spike time correlations across networks for synaptic integration and plasticity.

Notably, while the significant positive correlation between GABAergic inhibition and peak V1 gamma frequency agrees with two previous MRS-MEG studies17,18, the significant negative correlation we observed between GABAA receptor density and gamma amplitude was not observed in these studies. Interestingly, previous results have, in fact, shown that the administration of both alcohol32 and lorazepam33 may affect both the frequency and amplitude of gamma-band activity in the visual cortex. However, it should be noted that in the present study the statistical significance observed in the gamma amplitude correlation (rho = -0.7, p = 0.031) was weaker than the one we found for gamma frequency (rho = 0.74, p = 0.014). Besides, when regressing out the effect of gray matter density and size of V1, we observed that the gamma frequency correlation was actually enhanced (rho = 0.86, p = 0.0013), whereas the correlation between the receptor density and gamma amplitude only showed a trend to significance (rho = -0.56, p = 0.096). Furthermore, the Bayes factor correlation evidence results also confirm the statistical significance of the gamma-frequency-GABAA but not the gamma-amplitude- GABAA correlation (Table 1).

Previous reports have also suggested that the frequency of gamma-band activity in the visual cortex may depend on the surface area of V1, which, in turn, is linked to the horizontal connectivity and homogeneity within the region30. In the present study, correlations between gamma-band peak frequency and V1 surface area or volume did not reach significance (p = 0.084, in both cases). There are two possible explanations for this difference. First, in the study by Schwarzkopf et al.30, the surface area of V1 was defined using retinotopic mapping, whereas in our study the definition was based on a probabilistic atlas and the curvature of each subject’s cortex. Second, the definition of the surface area of V1 is only a surrogate of the homogeneity of V1, and would thus not necessarily correlate directly with the measured oscillation frequency. Interestingly, other studies have also failed to replicate the findings by Schwarzkopf et al.30 with respect to the relationship between V1 size and gamma properties34. Furthermore, our correlation analyses between FMZ-PET and alpha or beta oscillations provide no evidence for the influence of the GABAergic system on oscillatory amplitude and frequency in V1 at these lower frequencies. Thus, our data indicate that in V1 GABAergic inhibition primarily affects the properties of oscillatory neural responses in the gamma-band.

Exploring the neurochemical basis of neuronal oscillations, and its putative link to GABAergic inhibition in particular is a challenging endeavor35. A few recent studies were able, using MEG and MRS measurements, to link the concentration of GABA to the properties of oscillatory activity in the human brain17,18,19,20, and, specifically, to the peak frequency of gamma band-activity, in line with predictions from computational models of neural populations31. Interestingly, initial reports of correlation between GABA concentration and gamma oscillation frequency in human primary visual cortex17 were not replicated by a recent study24. The studies differed, among other things, in the number of participants, the type of MRS sequence used and the size of the MRS voxel of interest. Yet, irrespective of the reasons behind the differing results, the diverging findings between these studies but also from other reports21,22,23,36 do question the reliability of using MRS as a measure of the GABAergic mechanisms thought to mediate gamma oscillations24. MRS measurement of GABAergic inhibition suffers from susceptibility to macromolecule contamination and the need to sample large voxels. In addition, because MRS measures an overall GABA concentration level, it cannot be used to distinguish the contributions of neurotransmitter synaptic pool from the metabolic pool37. In fact, the quantification of GABA concentration following selective modulation with Tiagabine suggests that the MRS signal is only weakly affected by synaptic GABA38. This highlights the need to examine in vivo GABAergic inhibition and the GABA-gamma relationship with alternative techniques39. To this end, we chose Flumazenil-PET which allows quantification of GABAA benzodiazepine receptor density, a measure likely to be more directly related to the GABAergic inhibitory mechanisms involved in gamma oscillations; the correlation we found between gamma-frequency and GABAA receptor density in V1 supports the hypothesis that FMZ-PET measurements might indeed be better surrogate for GABAergic inhibition than MRS-based estimates. It should be noted, however, that although our findings were robust to numerous control analyses, they would certainly benefit from replication in a larger cohort. Sample size is a critical limitation in such correlation analyses and may potentially explain the discrepancies previously observed in MRS-based studies on the same question17,24.

In a sense, our results are consistent with both the Muthukumaraswamy et al.17 and the Cousijn et al.24 studies: The significant GABA-gamma frequency correlation replicates and extends results of the former study, and the fact that it has been unraveled using a technique more selective to GABAergic inhibition is in line with conclusions of the latter study. Moreover, discrepancies between MRS-based GABA-concentration and FMZ-PET-based GABAA receptor density measures are to be expected. It has been shown that vigabatrin may change the tissue GABA levels without an associated change in the benzodiazepine downregulation40, and TMS-based estimates of synaptic GABAA and GABAB activity do not necessarily correlate with the MRS-based concentration measures41,42,43.

Nevertheless, it is important to note that the claim made here about the potential higher suitability of FMZ-PET compared to MRS, only relates to the specific question of linking gamma oscillation properties to GABAergic inhibition. MRS-mediated GABA concentration measures provide highly relevant findings linking GABA levels to behavior. For instance, GABA concentration in frontal eye fields (FEF) predicts saccade distractibility44 whereas its concentration in supplementary motor area (SMA) is correlated with responsiveness of subconscious motor mechanisms45. Whether such explorations would benefit in any way from measuring GABAA receptor densities is an open question.

Finally, it is noteworthy that although FMZ-PET provides an assessment of GABAA receptor density rather than total GABA, neither FMZ-PET nor MRS-derived measures provide a direct quantification of inhibition strength. Flumazenil cannot be used to isolate a specific synaptic GABA-benzodiazepine receptor signal: The [11C]Flumazenil radioligand binds with similar affinity to synaptic (α1-α3) and to extrasynaptic (α5) subunits of GABA-benzodiazepine receptors. In contrast, recent findings indicate that the inverse agonist GABA-benzodiazepine receptor PET tracer [11C]Ro15-4513 can be used to quantify specific synaptic (alpha-1 subtype) signals46 and can detect acute increases in synaptic alpha-1 GABA37. Combining data from [11C]Ro15-4513 PET with MEG measurements could help fine-tune the relationship between GABAergic inhibition and gamma oscillations. Moreover, it should be noted that at present it is difficult to associate our findings with the extensive modeling, animal and imaging work that shows that increased GABAergic efficacy (as measured by the inhibitory postsynaptic current decay times) leads decreased gamma frequencies32,33,47,48,49. Our results suggest that the PET based GABAA receptor density measurement can serve as a proxy for the total inhibition strength that has been suggested to also affect the gamma frequency31. It is, however, unclear how the GABAA receptor density links to GABAergic efficacy and the various network kinematics properties that have been shown to influence the frequency of gamma oscillations8.

In conclusion, the present results show a prominent correlation between PET based measurements of GABAA receptor density and MEG based recordings of gamma-band oscillations in primary visual cortex. These results provide the first evidence in humans for a direct link between gamma oscillation properties and inhibitory neurotransmission indexed by GABAA receptor density. Moreover, our findings also suggest that Flumazenil based PET-GABAA estimates provide a spatially specific measure of GABAergic inhibition that, in combination with MEG measurements, could help elucidate the mechanisms that determine the properties of gamma-band oscillations and their variability across individuals in health and disease.

Methods

Subjects and experimental design

Thirteen healthy, native French-speaking subjects participated in the study. For one subject, the MEG recording failed due to technical reasons, and a second subject did not participate in the PET recording. In addition, the MEG data of a third subject had to be discarded due to excessive blinking. Both PET and MEG data of sufficient quality were thus recorded from 10 subjects (1 female, 9 males; age 19–29 years, mean 24 years). Informed consent was obtained from all subjects, in agreement with the prior approval of the Institutional Review Board and by the National French Science Ethical Committee (CPPRB). All methods were conducted in accordance with the CPPRB guidelines. The MEG experiment consisted of a classical working memory task of visually presented letters, with 3 s intervals between consecutive letters. Each letter was shown for 300 ms. The paradigm comprised three different memory load conditions (1-back, 2-back and 3-back tasks), and the subjects indicated with a button press, after each letter, whether the letter matched or did not match the appropriate preceding letter. Here, we focus on the initial average visual response across the three tasks for the non-target trials; we excluded the target-trials where the subjects’ behavior would have been markedly different with respect to the non-target trials. These trials and the early time-window contain the neural responses that reflect the perception and encoding of the presented letters to memory. The PET data used to determine the GABAA receptor density were collected while the subjects were resting eyes-closed in the scanner.

MEG, PET and MRI data collection

All data (MEG, PET and MRI) were collected from the same participants at the CERMEP imaging centre (Lyon, France). The MEG data were recorded in a magnetically shielded room using a 275-channel CTF whole-head system. The signals were band-pass filtered at 0.016-150 Hz and sampled at 600 Hz. The PET data were recorded with a Siemens HR + camera, and [11C]flumazenil (FMZ), an agent that binds to benzodiazepine (BZD) receptor, was used. FMZ (RO15-1788) was labeled with 11C, using the methylation process50. A dynamic 3D acquisition was applied providing 12 consecutive frames of 63 contiguous 2.42 mm thick slices, with an isotropic spatial resolution near 5 mm3 FWHM (full width at half-maximum). A 68Ge transmission scan was used to measure the attenuation correction. A partial saturation protocol consisting of a single intravenous injection of a mixture of 5 mCi of [11C]FMZ and 0.01 mg/kg of unlabeled FMZ was used, followed by acquisition of the emission data for 55 minutes. This single injection allowed the calculation of B′max parametric images, i.e., estimates of receptor density, without arterial blood sampling51. Anatomical MRIs were collected with a 1.5 Tesla scanner (Siemens Sonata Maestro Class).

MEG data analysis

Neural activity estimates were obtained from the MEG signals with event-related Dynamic Imaging of Coherent Sources (erDICS)52, a beamforming technique in the time-frequency domain. In erDICS, the cortical-level beamforming estimates are based on the sensor-level time-dependent cross-spectral density matrix (CSD), computed using Morlet wavelets53. Here, wavelets of width 7 were used to calculate the CSD in the range from 200 ms before stimulus onset to 800 ms post stimulus at 17 ms intervals; the width parameter defines both the spectral bandwidth and duration of the of the wavelets. The estimation of the time-frequency data was conducted by constructing wavelets at different frequency intervals at the different frequency ranges; the range from 4 to 15 Hz was sampled with 0.25 Hz resolution, whereas the range from 15 to 30 Hz and 36 to 104 Hz were sampled with 0.5 and 2 Hz resolutions, respectively. Trials in which the amplitude of either the vertical or the horizontal electro-oculogram exceeded 150 μV were rejected. In addition, the MEG data were examined visually and data segments that contained artifacts were excluded from the analysis. The number of accepted trials varied between 151–254 across subjects. The beamforming estimates of cortical oscillatory power levels at different frequencies were computed, using a spherical head model, at each time-instance, as an average of the time-bin itself and six preceding and following time-bins; the baseline activity level per frequency bin was estimated as the average power in the −200 to 0 ms baseline interval (total of 13 time-bins as well). For frequencies up to 30 Hz, the estimates were computed for individual frequency bins, whereas in the interval of 40–100 Hz, we averaged the data across an 8 Hz window at each bin to reduce the effects of noise (e.g., estimate of activity of 40 Hz was calculated based on the average CSD at the interval of 36–44 Hz). The general effects of the stimulation were evaluated at the group-level (paired t-test, p < 0.005, uncorrected) by comparing the average activity in the alpha (8–13 Hz), beta (15–25 Hz) and high-gamma band (60-90 Hz) between the pre-stimulus (-200 to 0 ms) and the post-stimulus (0 to 600 ms) time-windows. For visualization, the results were projected to the surface of the brain.

PET data analysis

Static PET images were obtained by summing frames 8–12 from the dynamic imaging (corresponding to the acquisition period from 20 to 55 min post-injection). These static images of the PET volumes were realigned to the anatomical MRIs. Ten millimeter circular ROIs were placed over the midportion of the pons, a suitable reference region for the calculation of the non-specific FMZ binding51; the reference encompassed 7–9 consecutive MRI slices displaying that structure. These ROIs were then transferred onto the corresponding FMZ-PET slices. An additional circular ROI with 15 mm diameter was placed over the occipital cortex which commonly displays a high concentration of BZD receptors54. A partial-saturation model, based on a Scatchard plot, was then used to obtain the B′max parametric images55. In this model, the free ligand concentration is estimated in the pons, whereas the range of the bound ligand concentration is evaluated in the occipital cortex. Using this approach we obtained, for each subject, a set of 63 contiguous 2.42 mm thick parametric images of BZD receptor B′max. Each individual’s PET data were smoothed using a 15 × 15 × 21 mm full width at half maximum Gaussian kernel; the level of smoothing was chosen to achieve similar resolution to the MEG estimates of neural activity56.

MEG-PET correlation analysis in the primary visual cortex

The MEG estimates were computed separately in each voxel of a grid that covered the gray matter surface of the brain. An equivalent spatial sampling across subjects was achieved by first forming a volumetric grid with 6-mm sampling in the template brain. A grid point was included in the analysis when it was within 1.5 cm of the brain surface. The template brain grid, consisting of 3922 points, was transformed, via an elastic transformation (implemented in SPM8; Wellcome Trust Centre for Neuroimaging), to each individual’s anatomy. The MEG beamforming estimates were calculated directly at these individual-level grid points. The PET-data were coregistered with each individual’s anatomical MRI in SPM8.

The MEG-PET correlation analysis was conducted in the bilateral primary visual cortex (V1). The anatomical definition of V1 (see Figs 1c, 2) was based on the automatic segmentation and parcellation procedure in Freesurfer 5.357; in the procedure, the bilateral V1 is defined based on a probabilistic atlas and subject-specific cortical folding patterns58. Following the segmentation of the gray matter surface and the determination of the V1 labels (left/right) in the surface space, the labels were transformed into volumetric data and the MR-voxels corresponding to the V1-gray matter surfaces were identified. With regard to the MEG data, we determined, for each subject, the grid points that belonged to V1, and collected the MEG estimates across these grid points. The amplitudes of each subject’s data were normalized by the standard deviation in all V1 grid points across all frequency bins in the baseline time-window; the normalized estimates were used to eliminate the effects of head and brain geometry on the amplitude estimates. This normalization was conducted separately for the low (4 to 30 Hz) and high-frequency (40-100 Hz) bands. For the PET data, we determined for each V1 MR-voxel the closest corresponding PET-voxel, and defined the unique set of these V1-PET voxels. The MEG responses were computed by subtracting the average activity during the baseline time-window from the post-stimulus time-bins, separately at each frequency; the MEG responses used in the MEG-PET correlation analysis were obtained by averaging the MEG responses across the V1 grid points. For each subject, we identified the peak frequency, timing and amplitude of the MEG responses, separately for the alpha- (7-14 Hz), beta- (15-30 Hz), and gamma-bands (40-100 Hz). The peak frequency was identified as the frequency showing the largest modulation between 0 and 600 ms; the peak timing was defined as the time-instance between 0 and 600 ms showing the largest modulation of activity in each band (alpha, beta, gamma); the peak amplitude was determined at the time-frequency bin corresponding to the peak frequency and time-instance. The amplitude measures are reported in arbitrary units as they have been normalized by the standard deviation across frequencies and V1 grid-points. As the GABAA estimate we used the total GABAA receptor density in the unique set of V1-PET voxels. The correlation between the PET-GABAA estimates and the MEG estimates were computed using Spearman’s rho.

We also evaluated the possibility of finding correlations between the V1-specific MEG estimates and PET-GABAA estimates in random voxel sets outside the visual cortex (selected from among voxels that were more than 4 cm from the primary visual cortex). Here, we selected 100 sets of fully random voxels and 100 sets of random contiguous voxels (clusters); for both set-types, an equal number of voxels to voxels in each subjects’ V1 were selected. We also investigated whether it would matter if one quantifies the modulations as an absolute or percent signal change between the post-stimulus and baseline time-windows. In addition to the MEG-PET correlation analysis, we also evaluated the correlation between the size of V1 (volume and surface area) and the MEG responses. Moreover, because FMZ-PET based measures of GABAA receptor density have been used as a surrogate marker for neuronal density29, it is possible that any observed relationship between GABAA density estimates and rhythmic activity could be due to inter-subject differences in neuronal volume of V1. Moreover, previous reports have suggested that the gamma-band frequency is dependent on the surface area of V130. To account for the contribution of these possible confounding factors, we applied linear regression to remove the effects of V1 surface area and gray matter density from the PET based GABA-A estimates and ran the main correlation analyses over again. Note that the gray matter density in V1 was determined by first computing the proportion of gray matter for each PET voxel and by averaging these values across V1. Unless mentioned otherwise, for all correlations analyses reported here between GABAA receptor density on one hand and oscillatory amplitude or frequency on the other, across the population, we used uncorrected p-values.

Finally, in addition to Spearman’s rank correlation analysis, we also computed Pearson’s correlation coefficient and a Bayes factor (BF) based on the approach described by Wetzels and Wagenmakers59. BF summarizes the ratio of evidence for a correlation of any kind against the evidence for no correlation (the null hypothesis). Despite the lack of clear-cut categorical boundaries, a BF < 0.3 is taken as an indication in favor of and a BF > 3 as strong evidence against the null hypothesis60.

As with low number of subjects the correlation estimates may be influenced by outliers in the data, we computed the 95% confidence limits for the correlation estimates using bootstrapping. In the approach we left out data pairs for 2 random subjects 200 times, and computed the correlation and p-values for the re-sampled data. From the obtained distribution of 200 correlation-values we calculated the 95% upper and lower confidence limits. This procedure was applied to all main correlation analyses.

Additional Information

How to cite this article: Kujala, J. et al. Gamma oscillations in V1 are correlated with GABAA receptor density: A multi-modal MEG and Flumazenil-PET study. Sci. Rep. 5, 16347; doi: 10.1038/srep16347 (2015).

References

  1. 1.

    , , & Visually induced gamma-band activity predicts speed of change detection in humans. Neuroimage 51, 1162–1167 (2010).

  2. 2.

    , , , & High-frequency neural activity and human cognition: past, present and possible future of intracranial EEG research. Prog Neurobiol 98, 279–301 (2012).

  3. 3.

    et al. How silent is silent reading? Intracerebral evidence for top-down activation of temporal voice areas during reading. J Neurosci 32, 17554–17562 (2012).

  4. 4.

    , , & Gamma oscillations in human primary somatosensory cortex reflect pain perception. PLoS Biol 5, e133 (2007).

  5. 5.

    , , & Temporal coding organized by coupled alpha and gamma oscillations prioritize visual processing. Trends Neurosci 37, 357–369 (2014).

  6. 6.

    & The gamma oscillation: master or slave? Brain Topogr 22, 24–26 (2009).

  7. 7.

    , , & Oscillatory activity of the human cerebellum: the intracranial electrocerebellogram revisited. Neurosci Biobehav Rev 37, 585–593 (2013).

  8. 8.

    & Mechanisms of gamma oscillations. Annu Rev Neurosci 35, 203–225 (2012).

  9. 9.

    et al. Task-related gamma-band dynamics from an intracerebral perspective: review and implications for surface EEG and MEG. Hum Brain Mapp 30, 1758–1771 (2009).

  10. 10.

    & High-frequency oscillations—where we are and where we need to go. Prog Neurobiol 98, 316–318 (2012).

  11. 11.

    , , & Oscillatory brain state predicts variability in working memory. J Neurosci 34, 7735–7743 (2014).

  12. 12.

    , , , & Neurophysiological investigation of the basis of the fMRI signal. Nature 412, 150–157 (2001).

  13. 13.

    , , , & Variability of the Relationship between Electrophysiology and BOLD-fMRI across Cortical Regions in Humans. J Neurosci 31, 12855–12865 (2011).

  14. 14.

    , et al. Multivariate analysis of correlation between electrophysiological and hemodynamic responses during cognitive processing. Neuroimage 92, 207–216 (2014).

  15. 15.

    , & Synaptic mechanisms of synchronized gamma oscillations in inhibitory interneuron networks. Nat Rev Neurosci 8, 45–56 (2007).

  16. 16.

    & Interneuron diversity series: inhibitory interneurons and network oscillations in vitro. Trends Neurosci 26, 676–682 (2003).

  17. 17.

    , , , & Resting GABA concentration predicts peak gamma frequency and fMRI amplitude in response to visual stimulation in humans. Proc Natl Acad Sci USA 106, 8356–8361 (2009).

  18. 18.

    , , & Orientation discrimination performance is predicted by GABA concentration and gamma oscillation frequency in human primary visual cortex. J Neurosci 29, 15721–15726 (2009).

  19. 19.

    , , & Relating MEG measured motor cortical oscillations to resting gamma-aminobutyric acid (GABA) concentration. Neuroimage 55, 616–621 (2011).

  20. 20.

    et al. GABA level, gamma oscillation, and working memory performance in schizophrenia. Neuroimage Clin 4, 531–539 (2014).

  21. 21.

    , , , & Neuronal network pharmacodynamics of GABAergic modulation in the human cortex determined using pharmaco-magnetoencephalography. Hum Brain Mapp 31, 581–594 (2010).

  22. 22.

    et al. Elevating endogenous GABA levels with GAT-1 blockade modulates evoked but not induced responses in human visual cortex. Neuropsychopharmacology 38, 1105–1112 (2013).

  23. 23.

    et al. Marked reductions in visual evoked responses but not gamma-aminobutyric acid concentrations or gamma-band measures in remitted depression. Biol Psychiatry 73, 691–698 (2013).

  24. 24.

    et al. Resting GABA and glutamate concentrations do not predict visual gamma frequency or amplitude. Proc Natl Acad Sci USA 111, 9301–9306 (2014).

  25. 25.

    et al. Pyramidal cell-interneuron interactions underlie hippocampal ripple oscillations. Neuron 83, 467–480 (2014).

  26. 26.

    , et al. Disrupting the clustering of GABAA receptor alpha2 subunits in the frontal cortex leads to reduced gamma-power and cognitive deficits. Proc Natl Acad Sci USA 110, 16628–16633 (2013).

  27. 27.

    & Control of hippocampal gamma oscillation frequency by tonic inhibition and excitation of interneurons. Nat Neurosci 13, 205–212 (2010).

  28. 28.

    , , , & Disruption of GABA(A) receptors on GABAergic interneurons leads to increased oscillatory power in the olfactory bulb network. J Neurophysiol 86, 2823–2833 (2001).

  29. 29.

    et al. Where in-vivo imaging meets cytoarchitectonics: the relationship between cortical thickness and neuronal density measured with high-resolution [18F]flumazenil-PET. Neuroimage 56, 951–960 (2011).

  30. 30.

    , , , & The frequency of visually induced gamma-band oscillations depends on the size of early human visual cortex. J Neurosci 32, 1507–1512 (2012).

  31. 31.

    & What determines the frequency of fast network oscillations with irregular neural discharges? I. Synaptic dynamics and excitation-inhibition balance. J Neurophysiol 90, 415–430 (2003).

  32. 32.

    , , & Acute effects of alcohol on stimulus-induced gamma oscillations in human primary visual and motor cortices. Neuropsychopharmacology 39, 2104–2113 (2014).

  33. 33.

    , , & GABAergic modulation of visual gamma and alpha oscillations and its consequences for working memory performance. Curr Biol 24, 2878–2887 (2014).

  34. 34.

    , , , & The properties of induced gamma oscillations in human visual cortex show individual variability in their dependence on stimulus size. Neuroimage 68, 83–92 (2013).

  35. 35.

    et al. Neurosystems: brain rhythms and cognitive processing. Eur J Neurosci 39, 705–719 (2014).

  36. 36.

    et al. The role of GABAergic modulation in motor function related neuronal network activity. Neuroimage 56, 1506–1510 (2011).

  37. 37.

    et al. Acute increases in synaptic GABA detectable in the living human brain: a [(11C]Ro15-4513 PET study. Neuroimage 99, 158–165 (2014).

  38. 38.

    , , , & Measurement of GABA using J-difference edited 1H-MRS following modulation of synaptic GABA concentration with tiagabine. Synapse 68, 355–362 (2014).

  39. 39.

    et al. Tiagabine increases [11C] flumazenil binding in cortical brain regions in healthy control subjects. Neuropsychopharmacology 34, 624–633 (2009).

  40. 40.

    et al. Effects of vigabatrin on the GABAergic system as determined by [123I]iomazenil SPECT and GABA MRS. Epilepsia 40, 1433–1438 (1999).

  41. 41.

    et al. Relationship between transcranial magnetic stimulation measures of intracortical inhibition and spectroscopy measures of GABA and glutamate + glutamine. J Neurophysiol 109, 1343–1349 (2013).

  42. 42.

    , & What are we measuring with GABA magnetic resonance spectroscopy? Commun Integr Biol 4, 573–575 (2011).

  43. 43.

    et al. Relationship between physiological measures of excitability and levels of glutamate and GABA in the human motor cortex. J Physiol 589, 5845–5855 (2011).

  44. 44.

    , , , & More GABA, less distraction: a neurochemical predictor of motor decision speed. Nat Neurosci 13, 825–827 (2010).

  45. 45.

    et al. Individual differences in subconscious motor control predicted by GABA concentration in SMA. Curr Biol 20, 1779–1785 (2010).

  46. 46.

    et al. Characterisation of the contribution of the GABA-benzodiazepine alpha1 receptor subtype to [(11)C]Ro15-4513 PET images. J Cereb Blood Flow Metab 32, 731–744 (2012).

  47. 47.

    , , , & Analysis of gamma rhythms in the rat hippocampus in vitro and in vivo. J Physiol 493 (Pt 2), 471–484 (1996).

  48. 48.

    , & Effects of intravenous anaesthetic agents on fast inhibitory oscillations in the rat hippocampus in vitro. Br J Pharmacol 118, 1977–1986 (1996).

  49. 49.

    , & Synchronized oscillations in interneuron networks driven by metabotropic glutamate receptor activation. Nature 373, 612–615 (1995).

  50. 50.

    , , , & Synthesis of ethyl 8-fluoro-5,6-dihydro-5-[11C]methyl-6-oxo-4H-imidazo [1,5-a] [1,4]benzodiazepine-3-carboxylate (RO 15.1788-11C): a specific radioligand for the in vivo study of central benzodiazepine receptors by positron emission tomography. Int J Appl Radiat Isot 35, 973–976 (1984).

  51. 51.

    et al. Quantification of benzodiazepine receptors in human brain using PET, [11C]flumazenil, and a single-experiment protocol. J Cereb Blood Flow Metab 15, 284–300 (1995).

  52. 52.

    , & A method for spatiotemporal mapping of event-related modulation of cortical rhythmic activity. Neuroimage 42, 207–217 (2008).

  53. 53.

    , , & Oscillatory gamma-band (30–70 Hz) activity induced by a visual search task in humans. J Neurosci 17, 722–734 (1997).

  54. 54.

    et al. SPECT imaging of the benzodiazepine receptor: feasibility of in vivo potency measurements from stepwise displacement curves. J Nucl Med 32, 1754–1761 (1991).

  55. 55.

    , , , & Parametric images of benzodiazepine receptor concentration using a partial-saturation injection. J Cereb Blood Flow Metab 17, 343–355 (1997).

  56. 56.

    , , , & Properties of MEG tomographic maps obtained with spatial filtering. Neuroimage 19, 1329–1336 (2003).

  57. 57.

    FreeSurfer. Neuroimage 62, 774–781 (2012).

  58. 58.

    et al. Accurate prediction of V1 location from cortical folds in a surface coordinate system. Neuroimage 39, 1585–1599 (2008).

  59. 59.

    & A default Bayesian hypothesis test for correlations and partial correlations. Psychon Bull Rev 19, 1057–1064 (2012).

  60. 60.

    Bayesian Versus Orthodox Statistics: Which Side Are You On? Perspect Psychol Sci 6, 274–290 (2011).

Download references

Acknowledgements

We thank the Academy of Finland (personal grant to JK), TES Foundation, KAUTE Foundation, and Oskar Öflund Foundation for financial support. This research was performed within the framework of the LABEX CORTEX (ANR-11-LABX-0042) of Université de Lyon, within the program ANR-11-IDEX-0007. This research was undertaken, in part, thanks to funding from the Canada Research Chairs program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author information

Affiliations

  1. Department of Neuroscience and Biomedical Engineering, Aalto University, 02150 Espoo, Finland

    • Jan Kujala
  2. Lyon Neuroscience Research Center, INSERM U1028—CNRS UMR5292, F-69000, Lyon, France

    • Jan Kujala
    • , Julien Jung
    • , Françoise Lecaignard
    • , Amélie Lothe
    • , Romain Bouet
    • , Carolina Ciumas
    • , Philippe Ryvlin
    •  & Karim Jerbi
  3. Department of Epileptology and Functional Neurology, Lyon Neurological Hospital, F-69000, Lyon, France

    • Julien Jung
  4. CERMEP imaging center, F-69003, Bron, France

    • Sandrine Bouvard
    •  & Françoise Lecaignard
  5. Institute for Child and Adolescent with Epilepsy (IDEE), F-69000, Lyon, France

    • Sandrine Bouvard
    • , Carolina Ciumas
    •  & Philippe Ryvlin
  6. Department of Clinical Neurosciences, CHUV, 1011, Lausanne, Switzerland

    • Philippe Ryvlin
  7. Department of Psychology, University of Montreal, H3C 3J7 Montreal, Québec, Canada

    • Karim Jerbi

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Contributions

J.J., F.L, C.C. and P.R. designed the experiments; J.J., S.B., F.L. and A.L. conducted the experiments; J.K. S.B., F.L., R.B. and K.J. analyzed the data; J.K., J.J., S.B., F.L., A.L., R.B., C.C., P.R and K.J. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Jan Kujala.

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