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Zoletti GO, Carmo FL et al. J Med Microbiol 2010; 59: 1360–1364

This study used PCR-denaturing gradient gel electrophoresis (PCR-DGGE). This is a culture independent, rapid fingerprinting technique for different nucleotide sequences has been used in many fields of microbial ecology. Samples were taken from 23 teeth during revisionary endodontics. Twelve of these teeth had radiographic evidence of apical periodontitis. The investigators found that 'no significant pattern of bacterial community profile was associated with root-canal-treated teeth with or without apical periodontitis.' Nevertheless they reported that some of the bands (indicative of bacterial species) were more prevalent than others. In addition, the mean number of bands were higher (p = 0.04) in those patients with apical periodontitis.