Abstract
Persistent hyperactivity of the Hippo effector YAP in activated satellite cells is sufficient to cause embryonal rhabdomyosarcoma (ERMS) in mice. In humans, YAP is abundant and nuclear in the majority of ERMS cases, and high YAP expression is associated with poor survival. However, YAP1 is rarely mutated in human ERMS. Instead, the most common mutations in ERMS are oncogenic RAS mutations. First, to compare YAP1 S127A and KRAS G12V-driven rhabdomyosarcomas, we re-analysed gene expression microarray datasets from mouse rhabdomyosarcomas caused by these genes. This revealed that only 20% of the up or downregulated genes are identical, suggesting substantial differences in gene expression between YAP and KRAS-driven rhabdomyosarcomas. As oncogenic RAS has been linked to YAP in other types of cancer, we also tested whether KRAS G12V alone or in combination with loss of p53 and p16 activates YAP in myoblasts. We found that neither KRAS G12V alone nor KRAS G12V combined with loss of p53 and p16 activated Yap or Yap/Taz-Tead1–4 transcriptional activity in C2C12 myoblasts or U57810 cells. In conclusion, whilst oncogenic KRAS mutation might activate Yap in other cell types, we could find no evidence for this in myoblasts because the expression of KRAS G12V expression did not change Yap/Taz activity in myoblasts and there was a limited overlap in gene expression between KRAS G12V and YAP1 S127A-driven tumours.
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Introduction
Rhabdomyosarcoma is the most common soft tissue sarcoma in children. The two main subtypes are alveolar rhabdomyosarcoma (ARMS), which is associated with PAX3/7-FOXO1 fusion genes, and embryonal rhabdomyosarcoma (ERMS), which is associated with mutations in common cancer genes such as HRAS, KRAS, NRAS, CTNNB1, PIK3CA and TP531.
The ubiquitously expressed Hippo pathway is a set of proteins that have been discovered as a result of tumour suppressor screens in the fruitfly (Drosophila melanogaster). At the core of the mammalian Hippo pathway lie two kinases with two isoforms each: MST1 (gene symbol STK4), MST2 (gene symbol STK3), as well as LATS1 and LATS2. These kinases phosphorylate and inhibit the transcriptional co-factors YAP (gene symbol YAP1) and TAZ (gene symbol WWTR1). Active YAP and TAZ bind to TEAD1–4 transcription factors to regulate sets of genes that typically increase proliferation and organ size and that promote tumourigenesis2.
There is substantial but scattered evidence linking the Hippo pathway to sarcomas3. Transgenic mice with mutations of Hippo members (i.e. mutants of Lats14, Nf2/Merlin5 and Mob16) all spontaneously develop various types of sarcoma. We previously found that the expression of the constitutively active human mutant YAP1 S127A in murine satellite cells causes ERMS-like tumours7. Additionally we found that YAP is nuclear and presumably active in 143 (≈73%) of 171 ERMS cases7. We also found that the abundance of the YAP paralogue TAZ in ERMS is associated with reduced survival and that TAZ can transform myoblasts8.
YAP and other Hippo members are rarely mutated in ERMS and other types of sarcoma1,9 as well as most other types of cancer10. If Hippo members are mutated, it is typically in the form of copy number aberrations. Specifically, copy number gains of YAP1 and of VGLL3 have been reported for sarcoma7,11. VGLLs and YAP can bind Tead transcription factors at the same site (demonstrated for Vgll1 and TEAD412) suggesting that they are related transcriptional co-factors. However, the frequency of Hippo gene copy number aberrations and other mutations is too low to account for the common nuclear localization of YAP in human ERMS7, suggesting that mutated cancer genes cause the activation of YAP and TAZ.
Supporting this notion are studies that link oncogenic RAS isoforms13,14,15, WNT members16, and mTOR mutants17,18 to the Hippo pathway and especially YAP activity. For instance, YAP is essential for neoplastic initiation and progression of KRAS-mediated pancreatic ductal adenocarcinoma (PDAC)15. YAP also bypasses KRAS addiction in PDAC13, and functionally substitutes KRAS in murine lung cancer model14 and mediates drug resistance to drugs that target oncogenic RAS signalling in human non-small cell lung cancer cells19. In KRAS G12V expressing human myoblasts, YAP has been shown to promote cell proliferation and inhibit apoptosis and myogenic differentiation in vitro and in murine xenografts in vivo20.
Because recurrent mutations of some of these genes are frequently found in ERMS1, it seems likely that YAP is activated through cross-talk with frequently mutated cancer genes. In addition, expression of KRAS G12V and knockout of p16 in isolated satellite cells results in the formation of rhabdomyosarcoma with pleomorphic features. The transcriptional profiles of these tumours recapitulate the gene expression signatures of human embryonal rhabdomyosarcoma21.
Based on the above, we hypothesize that oncogenic RAS mutations which are frequently found in ERMS1 activate YAP through unknown mechanisms. The aim of this project was therefore to test the hypothesis that KRAS G12V is sufficient to activate YAP in myoblasts and that YAP is essential for the tumourigenic effects of KRAS G12V in this cell type.
Results
The expression of YAP1 S127A alone in activated satellite cells results in the formation of ERMS in mice7. Also, KRAS G12V in combination with a Cdkn2a null mutation gives rise to pleomorphic rhabdomyosarcoma with a similar gene expression profile to embryonal rhabdomyosarcoma21 To investigate whether the gene expression in these two types of tumour is similar, we compared the gene expression profiles of the YAP1 S127A-driven ERMS7 with those of KRAS G12V-Cdkn2a null-driven ERMS21. This analysis revealed substantial differences in up-regulated genes between YAP1 S127A and KRAS G12V-induced rhabdomyosarcomas; approximately 20% (174 genes) of the genes, up-regulated in sarcomas compared to differentiated skeletal muscle, are identical in YAP1 S127A-driven and KRAS G12V-Cdkn2a null-driven ERMS (Fig. 1A).
To identify common features among the 174 genes that are up-regulated in both mouse RMS types we performed an enrichment analysis using TOPPgene (https://toppgene.cchmc.org/enrichment.jsp). This revealed that both YAP1 S127A and KRAS G12V-induced genes are associated with mitosis and the cytoskeleton. Genes induced in YAP1 S127A but not KRAS G12V-Cdkn2a null-driven ERMS are enriched for immune system-associated genes and the DNA packaging complex, as well as for nucleosomes and chromosomes. Genes induced by KRAS G12V-Cdkn2a null but not YAP1 S127A-driven ERMS are enriched for genes associated with cell differentiation, cell projections and neuronal differentiation plus junctions and focal adhesions (Table S4).
YAP1 S127A and KRAS G12V-Cdkn2a null-driven ERMS repress the expression of mostly different gene sets in sarcomas compared to differentiated skeletal muscle, but again approximately 20% (100 genes) of the down regulated genes are identical. An enrichment analysis of the 100 down-regulated genes revealed that YAP1 S127A and KRAS G12V-Cdkn2a null-repressed genes are enriched for differentiated skeletal muscle genes (Fig. 1A).
Next, we tested whether genes that are either up-regulated or repressed in both YAP1 S127A and KRAS G12V-Cdkn2a ERMS overlap with the top 100 genes, whose high or low expression has been associated with poor survival in 18,000 cases of human cancer22. 15 out of 174 genes (Fig. 1B) that are up-regulated in both YAP1 S127A and KRAS G12V-Cdkn2a-null induced rhabdomyosarcoma overlap with top 100 genes whose high expression is best associated with poor survival in 18,000 cases of human cancer. The enrichment is even greater in YAP1 S127A-driven ERMS, as 5 of YAP1 S127A-induced genes (BIRC5, FOXM1, TOP2A, TPX2, and CCNB1) are also genes whose high expression is most associated with poor survival across 18000 cases of human cancer22. These genes are associated with mitosis and cell proliferation. Only 2 (CBEP3 and SATB1) out of 100 genes (Fig. 1B) that are down regulated in YAP1 S127A and KRAS G12V-Cdkn2a-null induced rhabdomyosarcoma overlap with the bottom 100 genes whose low expression is best associated with poor survival in 18,000 cases of human cancer.
We then asked if genes that are either up regulated or repressed in both YAP1 S127A and KRAS G12V-Cdkn2a ERMS overlap with the 260 genes that have been identified as “significantly mutated” in cancer using a MutSigCV analysis10. Only 2 (CCNTB1 and RUNX1) out of 260 genes are genes that are both up-regulated by YAP1 S127A and KRAS G12V, and are significantly mutated in cancer10. In contrast, there is no overlap between genes that are repressed in both YAP1 S127A and KRAS G12V-Cdkn2a null-induced rhabdomyosarcoma, and genes that are significantly mutated in cancer10.
Next, to investigate whether KRAS G12V can alter Yap/Taz abundance or activity-related phosphorylation, we transduced C2C12 myoblasts with retroviruses expressing either control vector or KRAS G12V and then analysed Yap/Taz through Western blotting and immunohistochemistry. As shown in (Fig. 2A), KRAS G12V expression did not change Yap/Taz protein levels nor Ser112 (paralogous to serine 127 in humans) phosphorylation of Yap. To test whether KRAS G12V affects Yap localisation, we seeded C2C12 myoblasts expressing KRAS G12V or empty vector at high confluence to promote cytosolic localisation23 to see whether KRAS G12V makes Yap translocate to the nucleus. We found that KRAS G12V did not promote the nuclear translocation of Yap (Fig. 2B) or Taz (Fig. S1).
Next, to test whether KRAS G12V expression affects Yap/Taz transcriptional activity, we transduced C2C12 myoblasts with control and KRAS G12V vectors and then used a 8xGTIIC Yap/Taz Tead1-4 reporter as a readout for the transcriptional activity24,25. Measuring normalised luciferase activity from the Hippo reporter construct revealed that KRAS G12V expression significantly reduced Yap/Taz Tead1-4 transcriptional activity (Fig. 2C). Collectively, these data suggest that KRAS G12V alone does not activate Yap or Yap/Taz-Tead1-4 transcriptional activity in C2C12 myoblasts.
We also considered that the effects of KRAS G12V expression on Yap protein levels might be masked by high Yap activity because of the high serum content of the culture media26. We therefore knocked down Yap, which reduced Yap protein level (Fig. 3A) as well as Hippo reporter activity (Fig. 3B), and then transduced the cells with control vector or KRAS G12V retroviruses, However, KRAS G12V expression did not compensate the reduction in Yap protein levels and the Hippo reporter activity (Fig. 3A,B).
Stable expression of HRAS G12V induces cell senescence in primary human skeletal muscle cells20,27. To test whether KRAS G12V activates senescence signalling, we expressed either empty vector or KRAS G12V in C2C12 myoblasts and then measured p53 and p16 as senescence-associated proteins. We found that KRAS G12V significantly increased both p16 and p53 protein levels in C2C12 myoblasts28 when compared to the empty vector control (Fig. 4A). This suggests that KRAS G12V induces senescence-associated signalling in C2C12 myoblasts and this signalling might prevent the activation of Yap.
Therefore, to test the combinatorial effects of KRAS G12V expression and loss of senescence checkpoints, we knocked down p53 and p16 using CRISPR-cas9 and shRNA, respectively. We then overexpressed the small-T-antigen to generate a stable cell line with these mutations and verified the experimental manipulations by western blotting (Fig. 4B). We then expressed either control vector or KRAS G12V or empty vector in the genetically modified C2C12 myoblasts. As shown in (Fig. 4D), KRAS G12V expression did still not change Yap/Taz protein levels or the phosphorylation of Yap. Additionally, there was no change in the mRNA levels of Yap1, Wwtr1 (encoding Taz) or the Hippo target gene Cyr61 as shown by qPCR (Fig. 4E). Together, this suggests that even the expression of KRAS G12V together with the suppression of senescence checkpoints and small-T-antigen expression does not suffice to increase Yap/Taz Tead1-4 activity in myoblasts as judged by several assays.
To test whether this effect is specific to C2C12 myoblasts, we used U57810 cells, which were isolated from ERMS tumours arising in transgenic Myf6-Cre/p53−/− mice29. We first transduced these cells with control vector and KRAS G12V retroviruses. As shown in (Fig. 5A), there was no change in Yap, Taz or Yap S112 phosphorylation protein levels or Hippo reporter activity (Fig. 5B). We then generated a stable cell line as described previously (Fig. 4A) by knocking down p16 and overexpressed small-T-antigen before transducing the cells with either control vector or KRAS G12V retroviruses. Here, KRAS G12V significantly increased Yap/Taz protein levels when compared to control. However, there was a proportional increase in the phosphorylation of Yap at the inhibitory S112 site (Fig. 5C). Furthermore, there was no change in the Hippo reporter activity in KRAS G12V-transduced cells (Fig. 5D), suggesting that Yap/Taz-Tead1-4 activity was not increased. In summary, none of the experimental variations of KRAS G12V, small-T-antigen, p53 and p16 was sufficient to increase Yap/Taz- TEAD1-4 activity in C2C12 and U57810 myoblasts.
Discussion
A key dilemma in embryonal rhabdomyosarcoma is that YAP is expressed, nuclear and presumably active in the majority of human ERMS cases7 but that YAP1 is not significantly mutated in human ERMS which has a high frequency of oncogenic RAS mutations1 Given that interactions between mutated, oncogenic RAS and YAP have been reported for other cell and cancer types13,14,15, we tested for evidence that mutated, oncogenic KRAS can activate YAP in (rhabdo-) myoblasts to regulate the expression of a shared set of genes. Our main finding is that YAP1 S127A and KRAS G12V-Cdkn2a-driven rhabdomyosarcomas only share 20% of the up or down-regulated genes and that the expression of KRAS G12V together with various genetic manipulations of myoblasts does not activate Yap in these cells. This differs substantially from other types of cells and cancer, where oncogenic RAS isoforms cross-talk to and activate YAP13,14,15.
Reanalysis of gene datasets of YAP1 S127A-driven ERMS and KRAS G12V-Cdkn2a null-driven ERMS in mice showed that only 20% of up and down-regulated genes are the same in YAP1 S127A-driven ERMS and KRAS G12V-Cdkn2a null-driven ERMS7,21. The YAP1 S127A and KRAS G12V up-regulated genes include many genes involved with mitosis and the cell cycle such as the topoisomerase-encoding gene Top2a, centromere-related genes (Cenpa, Cenpe), or Plk4 which regulates centriole biogenesis30. Of these genes, Top2a was independently identified in a ChIP-Seq analysis as a direct YAP/TAZ target31. Additionally, YAP1 S127 and KRAS G12V up-regulated genes are enriched for genes that encode members of the cytoskeleton or focal adhesions such as the filamin FlnB, the actinin Actn1 or the actin-regulatory gene Capg. Conversely, YAP1 S127 and KRAS G12V down-regulated genes are often genes that are expressed in differentiated muscle such as the tropomyosin Tpm3, the myosin leight chain Mylpf and myoplalladin (Mypn). Collectively, this suggests that YAP and KRAS together both regulate the rhabdomyosarcoma core gene expression programme, which are genes associated with sustained proliferation whilst blocking terminal differentiation.
The role of the YAP and KRAS up-regulated cytoskeleton and focal adhesion-associated genes is less clear. However, there are some links between the cytoskeleton genes and Yap as the extra cellular matrix stiffness regulate Yap/Taz activity through cytoskeleton genes such as Rho and Actin25 and as Yap activity functions as mechano-regulator and influences the tissue tension32.
We additionally overlapped the YAP1 S127 and KRAS G12V up or down-regulated genes with the top or bottom 100 genes whose high or low expression is associated with poor survival in 18,000 cases of human cancer, respectively22. This is presumably the largest dataset linking the expression of individual genes to survival data pan-cancer. The overlap was 15 of the up-regulated and 2 of the down-regulated genes, suggesting that YAP1 S127 and KRAS G12V up-regulated mitosis and cell cycle-associated genes whose high expression is associated with poor survival pan-cancer. There was minimal overlap between YAP1 S127 and KRAS G12V-genes and genes that are significantly mutated pan cancer10.
Collectively, these findings reveal some limited similarities between YAP1 S127A and KRAS G12V-regulated genes but also that ≈80% of the target genes do not overlap. This contrasts other types of cancer where KRAS and YAP share a high proportion of target genes and are interchangeable. For example, in HCTtetK pancreatic adenocarcinoma cells, the expression of a mutant form of YAP1 rescues the expression of 77% of the genes that were decreased following KRAS knockdown14 suggesting that YAP and KRAS mostly regulate the expression of the same set of genes (77%) in pancreatic ductal adenocarcinoma (PDAC).
In contradiction to recent studies that linked Ras signalling and the Hippo pathway in a subset of tumours and cell lines33,34, the expression of KRAS G12V alone or in combination with loss of p53 and p16 was not sufficient to activate Yap in C2C12 myoblasts or in U57810 RMS cells. YAP is induced in a subset of KRAS-driven PDAC tumours following knockdown of KRAS and supports the growth of these tumours13. Additionally, in KRAS-driven lung cancer, acquired resistance to KRAS suppression was associated with increased YAP activity14.
Here, we found that ectopic expression of KRAS G12V reduced the transcriptional activity of Yap in C2C12 myoblasts as measured by Yap/Taz Tead1-4 luciferase reporter by 25% compared to control cells suggesting that KRAS G12V may negatively regulate Yap activity. However, we did not observe any changes in total Yap/Taz protein levels or their phosphorylation. In addition, there was no change in the localisation of Yap/Taz in C2C12 myoblasts cultured at high density. We hypothesized that the reduction in Yap/Taz transcriptional activity may be because the expression of RAS in human muscle cells has been shown to activate senescence check points as judged by increased p53 and p16 protein levels27,28. We indeed observed that the expression of both proteins (p53 and p16) is increased following the ectopic expression of KRAS G12V.
We therefore assumed that it is essential to knockdown both p53 and p16 in order to obtain a fully transformed cell line. We based that assumption on previously published studies where (H,K,N) RAS oncogenes in the presence of small-T-antigen overexpression and knockdown p53 and p16 knockdown have been shown to control YAP protein turnover and stability independently of the Hippo pathway in human primary BJ fibroblasts and human mammary epithelial cells33,34. The combination of both KRAS G12V and small-T-antigen expression in addition to p53 loss and p16 loss did not change Yap/Taz activity in C2C12 myoblasts. We speculate that Yap may not be directly activated by oncogenic KRAS but that Yap still is required for KRAS-mediated tumourigenesis. This is because Yap protein levels in AT1 cells isolated from KRAS-driven lung cancer were indistinguishable from control cells after KRAS induction for 6 weeks. However, at later stages Yap levels varied and correlated with tumour cell proliferation35.
In U57810 cells, which are murine ERMS cells isolated from transgenic p53 null mice in Myf6-positive differentiating myoblasts, the expression of KRAS G12V and small-T-antigen in addition to p16 knockdown led to an increase in Yap/Taz protein levels but there was a proportional increase in Yap S112/Taz S89 phosphorylation. In addition, there was no change in Yap/Taz transcriptional activity measured by Hippo Yap/Taz Tead1-4 luciferase reporter or Yap/Taz mRNA levels. This suggests that Yap/Taz are still under the control of upstream regulators of the Hippo pathway and that additional genetic alterations may be required in order to establish a direct link between Ras and Hippo signalling pathways. This also highlights the cell-type dependent regulation of the Hippo pathway36.
In summary, whilst there is evidence for a direct relationship between KRAS mutations and YAP activation in colon, pancreatic and lung cancer, this is not the case in (rhabdo-myoblasts) as KRAS G12V and YAP1 S127A drive mostly different gene expression programmes and that KRAS G12V expression alone or in combination of other genetic variations does not activate Yap/Taz in myoblasts.
Methods
Gene overlap analysis
The method of overlap analysis and of other bioinformatical analyses is described in the supplementary methods.
Cell culture
C2C12, mouse ERMS U5781 (isolated from transgenic mice harbouring loss of p53 in Myf6-positive differentiating myoblasts29) and HEK293T cells were cultured in Dulbecco’s minimum essential medium (DMEM, Sigma) supplemented with 10% foetal calf serum (Hyclone) and passaged when needed.
Retroviral and lentiviral expression vectors and transduction methods
pBABE puro KRAS G12V (Addgene plasmid #9052) and pBabe GFP Small-T-antigen (Addgene plasmid #10673) were used for the expression of KRAS G12V and Small-T-antigen respectively. pBABE-puro (Addgene plasmid #1764) was used as a control vector. p16 shRNA oligo (TRCN0000231227, Table S1 in supplementary methods) was designed using the Broad Institute RNAi consortium (http://portals.broadinstitute.org/gpp/public/seq/search), was used for p16 knockdown and subcloned into pLKO.1-blast, which was a gift from Keith Mostov (Addgene plasmid #26655). Retroviruses and lentiviruses were packaged in HEK293T cells as described previously37,38. Cells were transduced by incubation in diluted viral supernatant (1:4) until assayed.
Crispr-Cas9 knockdown of p53 and Yap
Protospacer sequences of CRISPR/Cas9 against p53 and Yap were designed by CRISPR DESIGN (http://crispr.mit.edu/). All specific target sequences were cloned into pX459 or pX330 vectors (Addgene plasmid #62988 and #42230 respectively). and verified by DNA sequencing. After the transient transfection of CRISPR/Cas9 into cells using Lipofectamine LTX reagent (Life Technologies), transfected cells were selected by DMEM culture medium supplemented with puromycin (2 μg/mL) (Sigma) for 48 hours and then expanded in regular culture medium. sgRNA oligo sequences targeting p53 and Yap are listed in (Table S2, supplementary methods).
Western blotting
Cells were lysed in modified RIPA buffer supplemented with protease and phosphatase cocktail inhibitors (Sigma). Whole cell lysate were separated via SDS-PAGE electrophoresis and transferred to 0.2 μM PVDF Western blotting membrane. Membranes were then probed with mouse anti-Yap/Taz (Santa Cruz, 101199, 1:100), rabbit anti-phospho Yap Ser127 (Cell Signaling, #9411, 1:1000) (this antibody can detect murine pYap S112 and pTaz S8925), pan-Ras (BD Bioscience, #610001, 1:1000), p53 (Cell Signaling, #2524, 1:1000), p16-INK4A (Proteintech, #10883-1-AP, 1:500), small-T-antigen (Millipore, #D01, 1:1000), tubulin (Sigma, #B512, 1:2000), actin (Sigma, #A5060, 1:2000) and Gapdh (Abcam, #Ab8245, 1:10000). Primary antibody binding was then visualised using species-specific conjugated secondary antibodies (Invitrogen) and digital imaging.
Immunocytochemistry
Fixed cells were permeabilized with 0.5% (v/v) Tween-20 in PBS for 6 minutes and blocked with 20% (v/v) goat serum in PBS for 30 minutes. Cells were incubated with primary anti-Yap (Santa Cruz, #101199, 1:50) and anti-Taz (Sigma, #HPA007415, 1:50) antibodies overnight at 4 °C. Species-specific fluorochrome conjugated secondary antibodies (Alexafluor, Invitrogen, 1:500) were added for 90 minutes at room temperature, and the slides were mounted with Vectashield medium with DAPI (Vector laboratories).
RNA extraction and quantitative RT-PCR
Total RNA was isolated from cultured cells using Trizol reagent (Invitrogen). cDNA was synthesized by reverse transcription using Superscript II (Invitrogen) and subjected to real-time PCR with gene-specific primers in the presence of 1x Light Cycler 480® SYBR green master (Roche). Relative abundance of mRNA was calculated by normalization to Gapdh (Table S3, supplementary methods).
Luciferase reporter assays
For the luciferase reporter assay, retrovirally transduced C2C12 myoblasts were seeded in 6-well plates overnight. The 8xGTIIC Yap/Taz Tead1-4 luciferase reporter (Addgene plasmid #34615) was co-transfected with TK-renilla plasmid. 24 hours after transfection, we lysed the cells and measured luciferase activity using a Dual-Luciferase Reporter Assay System (Promega) following the manufacturer’s instructions. All luciferase activities were normalized against renilla activity.
Statistical analysis
We analysed the data through GraphPad Prism (v5.0, GraphPad Software) and present the data as means ± S.D. Statistical comparisons were done using an unpaired Student’s-t-test. For all experiments, statistical analysis was conducted on raw data collected from at least three independent experiments performed on different occasions with three replicates each. A p-value of 0.05 or less was considered significant.
Change history
06 May 2019
The version of this Article previously published quoted an incorrect email address for Abdalla D. Mohamed. Correspondence and requests for materials should be addressed to abdalla.a.diaai@gmail.com. This has now been corrected in the HTML and PDF versions of the Article.
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The authors acknowledge funding from Sarcoma UK, grant number: SUK09.2015.
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A.D.M., N.V. and H.W. directed the project, designed the experiments and analysed the data and wrote the manuscript; A.D.M. and N.S. performed the experiments and discussed the results; S.H. discussed the results and wrote the manuscript.
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Mohamed, A.D., Shah, N., Hettmer, S. et al. Analysis of the relationship between the KRAS G12V oncogene and the Hippo effector YAP1 in embryonal rhabdomyosarcoma. Sci Rep 8, 15674 (2018). https://doi.org/10.1038/s41598-018-33852-7
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DOI: https://doi.org/10.1038/s41598-018-33852-7
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