Abstract
When in equilibrium, thermal forces agitate molecules, which then diffuse, collide and bind to form materials. However, the space of accessible structures in which micron-scale particles can be organized by thermal forces is limited, owing to the slow dynamics and metastable states. Active agents in a passive fluid generate forces and flows, forming a bath with active fluctuations. Two unanswered questions are whether those active agents can drive the assembly of passive components into unconventional states and which material properties they will exhibit. Here we show that passive, sticky beads immersed in a bath of swimming Escherichia coli bacteria aggregate into unconventional clusters and gels that are controlled by the activity of the bath. We observe a slow but persistent rotation of the aggregates that originates in the chirality of the E. coli flagella and directs aggregation into structures that are not accessible thermally. We elucidate the aggregation mechanism with a numerical model of spinning, sticky beads and reproduce quantitatively the experimental results. We show that internal activity controls the phase diagram and the structure of the aggregates. Overall, our results highlight the promising role of active baths in designing the structural and mechanical properties of materials with unconventional phases.
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Main
In the classical picture of Brownian motion, the incessant motion of microscopic particles results from collisions with the fluid molecules. The particles and solvent are passive, driven by thermal fluctuations1,2. Agitated molecules diffuse, interact and collide, building materials. This view of assembly constituted an elemental inspiration for colloidal science, which aims to translate the versatility of chemistry to the microscale. It led to the design of a broad library of building blocks (1–10 μm in size) with various shapes and chemical properties to mimic chemical bonds3. A major obstacle remains, as thermal energy is not sufficient to allow micrometric particles to explore the conformational space efficiently, making assembly challenging and often elusive4,5. In living systems, assembly is assisted by molecular motors that generate active fluctuations6,7 and enhance intracellular transport8,9. Active agents present in a solvent generate forces and flows, adding active noise to thermal fluctuations. They constitute an effective medium, an active bath, that can, in principle, overcome kinetic barriers and control the (non-equilibrium) assembly of passive building blocks. Libchaber coined the term bacterial bath to describe the effect of swimming Escherichia coli on the positional fluctuations of micron-scale tracers10, later extended to suspensions of self-propelled particles, namely nanorods or bacteria at different concentrations and speeds11. Active baths can be defined with an effective temperature as hot thermal systems under certain conditions12 but remain intrinsically non-equilibrium, featuring properties prohibited by thermal physics. Active baths produce work13, power asymmetric gears14,15 and modulate effective interparticle interactions16,17. Yet, the use of active baths to control assembly has largely been unexplored.
Here, we investigate the aggregation of sticky colloids in an active bath of swimming E. coli bacteria. We show that the bacterial bath presents features of a hot thermal bath, effectively enhancing the dynamics of assembly. We further report that the aggregates exhibit a slow and persistent clockwise rotation, which makes the bacterial bath effectively chiral and controls the morphology and phase diagram of aggregation. Our results are quantitatively reproduced by a minimal numerical model of attractive spinners, demonstrating the importance of the non-equilibrium rotation and folding in the reshaping of aggregates and the structuring of gels. We further highlight how the mechanical properties of such materials, assembled in active baths, differ from conventional, thermal ones.
The experiment used colloidal beads (2.2-μm 3-(trimethoxysilyl)propyl methacrylate spheres) immersed in a suspension of swimming E. coli bacteria. Short-range attractions between the beads were produced using a conventional depletion interaction with a non-absorbing polymer as depletant, namely poly(ethylene oxide) (PEO) with a molecular weight (MW) of 600,000 Da. We performed our experiments with an attraction strength of ~75 kBT, where kB is the Boltzmann constant and T is the ambient temperature, which effectively led to irreversible binding of the colloidal beads after collisions, dubbed sticky colloids (Methods). A solution of sticky colloids, that is colloidal beads and depletants ([PEO] = 3.25 g l−1), was added to either a pure motility medium (the thermal bath) or swimming E. coli bacteria suspended in the motility medium, at concentration ρB (the active bath), and sealed in a glass capillary (Supplementary Information). To keep the activity of the bath constant in anaerobic conditions, we added 1% w/v glucose to the suspension, enabling the E. coli to swim at a constant speed, V = 23 μm s−1 ± 2 μm s−1 throughout the 4-h duration of an experiment (Supplementary Fig. 2)18,19. The concentration ρB of E. coli bacteria in the bath was controlled via optical density and adjusted by centrifugation and resuspension for each experiment (Supplementary Fig. 1). We examined active baths with various bacterial concentrations ρB, such that the maximal value ρ* = 6 × 108 cells per millilitre was an order of magnitude below the onset of bacterial turbulence20.
The glass capillary containing the sticky beads was laid on the programmable stage of an optical microscope and observed (Fig. 1a). The particles sedimented to form a near two-dimensional (2D) system, at constant surface fraction ΦS ≈ 18 ± 3%. Colloidal beads in the passive bath were agitated thermally. They collided and bound together, forming ramified aggregates resembling those obtained in diffusion-limited colloidal aggregation (DLCA)4. The system reached a near steady state in the experiments when large clusters diffused too slowly to continue growing. Similar experiments performed in the active bath of swimming E. coli bacteria revealed an entirely different dynamical state. The aggregates appeared more agitated and grew faster than in the thermal experiments. They were also visually distinct, being more compact than the ramified aggregates of the thermal experiment and presenting cavities (Fig. 1b and Supplementary Video 1).
We compared the dynamics of the aggregates in the thermal and active baths by tracking the aggregates. Collisions between aggregates were notably identified by abrupt changes in area and perimeter, and these led to novel aggregates. In addition to the translational dynamics of the centre of mass, we used the anisotropy of the aggregates to track their orientation and quantify their angular dynamics (Supplementary Information). Due to the complex and evolving shapes of the aggregates, a coarse-grained approach was required for meaningful comparisons. Thus, we characterized the size of the aggregates by their radius of gyration RG, as conventionally done for fluctuating polymers (Supplementary Information). The displacements were averaged over time and isotropically in space over long trajectories (>1 min), which allowed us to characterize the dynamics of the aggregates, for both translations and rotations, by their size RG and the concentration of the bacteria suspension ρB (Fig. 2).
In both the thermal and active baths, the mean squared displacement of the aggregates was linear at short times (Δt < 5 s), ΔR2(Δt) = 4DeffΔt, indicative of diffusive motion with effective diffusivity Deff (Fig. 2a, inset). The diffusivity in the thermal bath was lower than the Stokes–Einstein prediction for bulk diffusivity in water, a result of the increased viscosity of the motility medium with suspended depletant polymers21 and the added hydrodynamic dissipation from the proximal glass substrate of the capillary22. We further observed that Deff increased with bacterial concentration ρB, being up to eightfold larger than in the thermal bath. At fixed bacterial concentration ρB, the diffusivity decreased with RG, following the Stokes–Einstein scaling for a thermal system Deff = α(ρB)/RG, with α(ρB) a function of ρB extracted by fitting the data (Fig. 2a). Although non-monotonic behaviour of the diffusivity of spheres in a bacterial suspension has been previously reported23, this was only studied over a narrow range of sizes and the results are compatible with our observations for aggregates of complex shapes and varying sizes. Next, we studied the orientational dynamics of the aggregates. The dynamics of the orientation θ were similarly diffusive at short times, with \({{\Delta }}{\theta }^{2}({{\Delta }}t)=2{D}_{{{{\rm{eff}}}}}^{\theta }{{\Delta }}t\) and \({D}_{{{{\rm{eff}}}}}^{\theta }\) the angular diffusivity (Fig. 2b, inset). As for translational diffusion, \({D}_{{{{\rm{eff}}}}}^{\theta }\) increased with increasing bacterial concentration ρB and followed the Stokes–Einstein scaling: \({D}_{{{{\rm{eff}}}}}^{\theta }=\beta ({\rho }_\mathrm{B})/{R}_\mathrm{G}^{3}\), with 𝛽(ρB) a function of ρB extracted by fitting the data (Fig. 2b).
To compare the amplitude of the fluctuations that led to the translational and rotational diffusion in the active bath, we normalized the measured diffusivities by the value in the thermal bath and plotted α(ρB)/α0 and β(ρB)/β0, where α0 = α(0) and β0 = β(0) refer to the thermal system (Fig. 2c). Two comments are in order: (1) The normalized fluctuations α/α0 and β/β0 collapsed, highlighting that the fluctuations of the active bath were the common origin of the observed translational and rotational diffusion. (2) The normalized fluctuations scaled linearly with bacterial concentration ρB. These results agree with previous reports of the enhanced translational diffusivity of individual spheres in an active suspension11 and extend the conclusions to tracers with complex shapes and a broader range of sizes. The same holds for the rotational dynamics. Thus, ρB is a relevant experimental parameter for controlling the activity of a bacterial bath.
Our observations of the aggregates over tens of minutes revealed a small but persistent angular rotation, \(\varOmega\) < 10−2 rad s−1, which was previously imperceptible at shorter times (Fig. 2d and Supplementary Video 2). The rotation was consistently clockwise in experiments performed in the capillary (Fig. 2e–h), at a rate that increased with the bacterial concentration ρB and decreased with aggregate radius RG (Fig. 2i, inset). Remarkably, the data collapsed onto a master curve, \({{\varOmega }}/({{{\mathcal{M}}}} {\rho }_\mathrm{B}) \propto 1/{R}_\mathrm{G}^{3}\), where \({{{\mathcal{M}}}}\) is the mass of an aggregate particle (Fig. 2i). This indicates that a net torque \(\tau \propto {{{\mathcal{M}}}} {\rho }_\mathrm{B}\) was imparted from the active bath to the aggregates and balanced by a viscous torque proportional to \(1/{R}_{G}^{3}\). This linear dependence of the torque on the bacterial concentration ρB demonstrates the cumulative effect of the bacteria bath and led to the rotation of the aggregates in a persistent (clockwise) direction. It notably departs from the non-chiral rotation that arises from the summation of random contributions from bacteria in an aggregate24. By extracting the mobility μR from the measurements of rotational diffusivity in the thermal bath (Fig. 2b), we estimated the net torque exerted by the bacterial bath, \({\tau }_{{R}_\mathrm{G}}={\mu }_\mathrm{R}^{-1} {{\varOmega }}({R}_\mathrm{G}) \approx 0.4\) pN μm for RG ≈ 7 μm. This minute torque became noticeable in aggregation experiments lasting a few hours. We intuit that the rotational symmetry-breaking originates from the native chirality of E. coli flagella, which resulted in clockwise circular trajectories with typical radius of curvature RB ≈ 43 μm (Supplementary Fig. 3) near the no-slip boundary of the glass capillary25. Indeed, we could reverse the direction of rotation of the aggregates by performing experiments at the air–water interface of a pendant drop, where the direction of rotation of the bacteria reverse so that they move in counterclockwise circles26 (Supplementary Fig. 4). This confirms that the chirality of E. coli flagella breaks the rotational symmetry and makes the bacterial bath effectively chiral.
To understand the phenomenon better, we considered a toy model of a circular aggregate of radius a in an active bath of persistent self-propelled rods. For simplicity, the self-propelled particles exert a constant force F0 aligned with their propulsion direction (Supplementary Information and Supplementary Fig. 5). When the self-propelled particles navigate along straight segments, no net torque is exerted due to symmetry and the circular aggregate does not show persistent rotation14,15. In contrast, when the self-propelled particles move along circular clockwise trajectories, a wedge of unbalanced collisions appears. Its opening angle 2θ is controlled by the curvature 1/RB of the trajectories, θ ≈ 1/RB (Supplementary Fig. 5). The asymmetric collisions lead to a tangential force of amplitude F// ∝ 2θ × F0, which exerts a torque on the circular aggregate. The total torque, \({\tau }_{{R}_\mathrm{B}}\propto ({F}_{0}/{R}_\mathrm{B}) {\rho}_\mathrm{B}{a}^{2}\), is obtained by integration over the perimeter and multiple collisions and leads to clockwise rotation of the circular aggregate. Thus, this simple toy model highlights that an active chiral bath exerts an active torque on a circular aggregate, which is absent for an active bath of straight swimmers. We further extend this result, showing that an active bath that results in constant tangential forces along the boundary of an aggregate produces a torque proportional to \(\rho {{{\mathcal{A}}}}\), that is, proportional to the surface area \({{{\mathcal{A}}}}\) of the aggregate (Supplementary Information). Remarkably, this scaling, valid for aggregates of arbitrary shapes, agrees with the experimental results (Fig. 2i). We do, however, stress that, quantitatively, the rotation of the aggregates in the experiment is certainly more complicated than in this simple model, as the rotation is due to a superimposition of the effects of the complex shape of the aggregates, which can be locally asymmetric, as well as the forces exerted by the bacteria navigating inside the aggregate, as visible in Supplementary Fig. 3c. A quantitative description of these effects lies beyond the scope of this paper and constitutes further work.
Next, we considered the morphology and statistical properties of the colloidal aggregates formed in the thermal and active baths. To account for the accelerated dynamics of the active bath, we rescaled time so that the aggregates in the thermal and active baths reached the same average size. Aggregates formed after 25 h (thermal bath) or 1.25 h (active bath) reached comparable sizes, \({{{\mathcal{M}}}}=150\) colloids, but were different to the naked eye (Supplementary Fig. 6). The thermal aggregates were ramified, as is typical of DLCA, due to the low probability that a diffusing particle would reach the centre of the aggregate. In contrast, the aggregates in the active bath were compact and had cavities. Remarkably, aggregates that formed in either the thermal or active baths fall into distinct groups based on the fraction of colloids at the perimeter, a salient control parameter in the mechanical response of gels27 (Supplementary Fig. 8). To quantify the morphological changes further, we computed the fractal dimensions ν of an ensemble of aggregates, as \(\mathcal{M}_{i} \propto {R}_{\mathrm{G},i}^{\nu }\), where \({{{{\mathcal{M}}}}}_{i}\) is the mass, or number of colloids, in aggregate i and RG,i is the radius of gyration of aggregate i (Fig. 3a). Aggregates for which ν = 1 are elongated, whereas those with ν = 2 are compact. Intermediate values of ν are indicative of ramified, fractal structures. The fractal dimensions of the aggregates in the thermal bath and the active bath evolve in time before reaching a plateau after approximately 2 h (Supplementary Fig. 9). The value of the fractal dimension measured in the thermal bath, ν0 ≈ 1.4, agrees with values reported for DLCA in 2D28. As observed by the naked eye, aggregates in the active bath were more compact than those in the thermal bath, reaching ν(ρ*) ≈ 1.8 for the highest activity level.
The aggregate morphology was further quantified via the normalized complementary cumulative size distribution \({{{\mathcal{P}}}}\), corresponding to the probability for an aggregate to be larger than a given size (Supplementary Information). In the thermal bath, the size distribution of aggregates can be rescaled by the mean value, such that at all times the distributions collapse onto a single log-normal distribution (Supplementary Fig. 10), a universal feature of merging and fragmenting systems29,30. In contrast, the size distribution of aggregates formed in the active baths cannot be collapsed and exhibits significant deviations from log-normal. Notably, the tails become more prominent with increasing activity (Fig. 3b). Thus, aggregation in the thermal and active baths do not belong to the same universality class, highlighting a profound effect of the bacterial bath on the aggregation properties29,30. We intuit that those non-equilibrium properties originate in the compaction and folding of the aggregates, which are due to the observed rotation (Fig. 4a).
To understand the mechanism better, we developed a minimal physical model that aims to capture the chirality of the bacterial bath without explicitly considering the swimming bacteria. We expected the chiral bath to cause the aggregates of colloids to rotate, and we modelled the system as an ensemble of attractive rotating beads coupled to each other by friction (Fig. 3c and Supplementary Information). Rotation was implemented in a mean field fashion by attributing a constant torque to each bead. These molecular dynamics simulations were similar in spirit to previous models of internally driven gears31,32 with the addition of strong attraction between the spinners. The latter leads to irreversible aggregation, a notable difference from crystals of rotating cells reported at moderate attraction33. The clockwise rotation of the beads was implemented via an internal driving torque whose amplitude controls the internal activity of the system, thus allowing comparisons between the simulations and our experimental results. The numerical simulations capture visually the difference between aggregation in the thermal and active baths (Fig. 3d,e and Supplementary Videos 3–5). They, furthermore, reproduce quantitatively the experimental increase in fractal dimensions and the size distributions of the aggregates (Fig. 3f,g), thus validating the predictive power of the model. The aggregates rotated and grew faster than with thermal aggregation, in line with a simple analytical model of aggregation and collision that accounts for the sweeping effect of the rotation on the collision rate (Supplementary Fig. 11). The simulations, like the experiment, show two different effects of the rotation: (1) the mentioned sweeping motion of spinning aggregates and (2) the self-folding of the aggregates (Fig. 4b and Supplementary Video 6).
To disentangle their role, we compared our initial set of simulations, where aggregates can deform as a result of their internal torques (Fig. 4c), with another set where the aggregates were treated as rigid objects (Fig. 4d). At low internal torque, the simulations of both deformable and rigid aggregates formed 2D gels (Fig. 4c,d). The structures obtained were, however, markedly different. The gels formed by the percolation of deformable aggregates were structurally heterogeneous, with both compact and ramified regions (Fig. 4e). The gels built from rigid aggregates appeared more uniform in structure (Fig. 4g). At sufficient internal torque, the simulations of rigid aggregates produced space-spanning networks that resemble thermal DLCA gels (Fig. 4g,h). In contrast, the deformable aggregates do not percolate at high internal torques, as the internal torque folds each large, deformable aggregate onto itself. This leads to large compact clusters with internal cavities (Fig. 4f) and prevents the formation of a percolated network (Fig. 4c). The rotation enhances the dynamics of aggregation while the self-folding of the ramified aggregates controls their fractal dimension. This mechanism highlights the difference in the nature of the reported aggregation with conventional reaction-limited colloidal aggregation and leads to different morphologies4.
We next investigated the phase behaviour of aggregation for various surface fractions Φ and internal torques. We observed a gel phase and a cluster phase controlled by the activity (Fig. 5a). The (ν,Φ) plane was obtained by measuring the fractal dimension of the aggregates, which allowed us to characterize the experimental data in the bacterial baths (Fig. 5a inset and Supplementary Fig. 12). The gel phase was separated from the cluster phase by a critical fractal dimension \({\nu }_\mathrm{C} \approx d+\alpha \log {{\varPhi }}\), where d = 2 is the dimension of space and α ≈ 0.29 is phenomenologically adjusted to the data, the scaling in \(\log {{\varPhi }}\) originating from the definition of \({{\varPhi }}\propto {{{\mathcal{M}}}}\) (ref. 5). Overall, sufficient activity suppressed the gel phase in favour of compact aggregates.
Having established that the activity of the bath controls the phase behaviour of aggregation into a cluster phase or a gel phase, we next investigated the structural and mechanical properties of each phase. We observed for the cluster phase that the porosity of aggregates is controlled by the internal activity. Increasing the internal torque augments the compaction of the aggregates, leading to dense and compact structures (Fig. 5b). We considered 2D gels (at Φ = 0.31) assembled in a thermal bath or with internal activity. We probed those passive gels mechanically by numerically measuring the stress and strain (Supplementary Videos 7 and 8). Gels obtained by aggregation in the thermal bath exhibit conventional elastic behaviour in the limited range of the strain considered, namely ~10%. By contrast, gels assembled due to internal activity were highly nonlinear and behaved like mechanical diodes: elastic in compression and stiff under extension (Fig. 5c). The onset of the plastic regime also appeared to be controlled by the activity of the assembly.
To gain insights into the origin of this unconventional mechanical behaviour, we performed additional experiments and simulations, at Φ = 0.39, a surface fraction for which we can experimentally achieve a 2D percolated network greater than 1 mm × 1 mm in size in both the thermal and bacterial baths. We used bacterial concentrations of up to ρB = 0.1 ρ*, beyond which the gel phase is replaced by the cluster phase. Once again, there was good agreement between our experiments and the phase diagram predicted numerically (Fig. 5a). The gels produced in the active baths had significant structural differences compared to those achieved thermally (Fig. 6a,b). This could be seen as surprising, considering that the added fluctuations of the bacterial bath were comparable to the thermal energy itself (Fig. 2c). It simply highlights that the bacterial bath is more than a mere hot bath and that the acquired rotation of the aggregates has a profound effect on the dynamics and structure of the aggregates, as already apparent from the anomalous size distributions (Fig. 3b,g).
We quantified the structural differences by measuring the density fluctuations of the gels (Fig. 6c). We can define a typical length scale for the gel structure by identifying the local maximum of the number fluctuations σ2(N)/〈N〉 (ref. 34). As visible to the naked eye, the length scale grows from ~10 colloid diameters in the thermal gel to ~30–40 colloid diameters in the gels assembled in the bacterial bath. Additionally, the emergence of giant number fluctuations for gels assembled in the active bath highlight that the structures were more heterogeneous compared to those in the passive bath. To further quantify this aspect, we characterized the pores of the 2D gels by computing the distance from points in a pore to the nearest colloid in the network backbone35, as plotted in Fig. 6d. Gels assembled in the active baths had broader distributions than gels assembled thermally, indicative of large heterogeneous voids in the structure. These structural heterogeneities arise from the rotation of the aggregates induced by the chiral bacterial bath. The rotation results in the ramified aggregates jamming into each other and the formation of large isostatic structures (Fig. 6b, inset). Remarkably, the results from our simple numerical model of rotating aggregates with internal torques is again in good agreement with the results from our experimental realization of gels (Fig. 6g,h). This highlights how rotation and folding of the aggregates are the key ingredients in shaping and forming heterogeneous structures and driving exotic mechanical responses, in line with the role of structural heterogeneities in determining the breaking and restructuring of gels36,37.
Previous works reported that the moderate substitution of passive particles by active particles facilitates the annealing of colloidal monolayers38,39 or can control the yield stress of gels40,41. Our results highlight how the assembly in an active bath directs the structural and mechanical properties of materials entirely made of passive constituents. We are hopeful that our results will stimulate future work and lead to a complete microscopic model that translates aggregation in bacterial baths into the design of gels with unconventional mechanical responses.
In summary, we have demonstrated that active baths are a potent instrument for producing unconventional aggregates and gels and that activity is a salient parameter for phases of matter out of equilibrium. It is noticeable that simulations of a purely active system of spinners quantitatively reproduced experimental results of passive particles in a chiral bacterial bath, possibly highlighting generic features between these two classes of systems. The importance of chirality in morphogenesis42,43,44 further hints at the potential of chiral active baths to produce materials beyond what can be achieved thermally. Although our study was limited to 2D, it is a proof of concept for materials powered from within, in phases and structures not achieved via thermal treatments, as also recently highlighted for the effect of activity on liquid interfaces45. It will motivate efforts to assemble 3D materials in active baths, whereas the characterization of their non-conventional mechanical and rheological properties will drive further experimental and theoretical work. This work effectively lays out a road map for establishing the concept of bacterial forging, in which gels structures are controlled by the bacterial bath, the way forging in metallurgy controls the properties of metals through sequences of annealing and quenching. Because activity can, in principle, be controlled in time and space externally46, our findings open up a branch of materials science in which the properties of passive materials are tailored during their assembly in active baths and active materials are supplied with energy as desired, to induce healing or annealing.
Methods
Stock solutions
For all stock solutions, chemicals were dissolved in deionized (DI) water with a resistivity of 18 MΩ cm from a Milli-Q EQ 7000 water purification system. A stock solution of 200 mM NaCl (Sigma-Aldrich, MW 58.4) was prepared by dissolving 0.12 g of NaCl in 10 ml of DI water. A stock solution of 2% w/v F108 (Sigma-Aldrich Synperonic F108 surfactant, MW 14,600) was prepared by dissolving 0.2 g of F108 in 10 ml of DI water. A stock solution of 25 g l−1 PEO (Sigma-Aldrich, MW 600k) was prepared by dissolving 0.25 g of PEO in 10 ml of DI water, and stirred overnight using a magnetic stirring rod until dissolved. A stock solution of 0.1 M potassium phosphate buffer, used to prepare the motility medium, was prepared by dissolving 9.34 g of K2HPO4 (Sigma-Aldrich, MW 174.2) and 6.31 g of KH2PO4 (Sigma-Aldrich, MW 136.1) in 1 l of DI water. Then, 0.5 M of ethylenediaminetetraacetic acid (EDTA) stock solution was prepared by dissolving 186.10 g of EDTA dihydrate (Sigma-Aldrich, MW 372.2) into 1 l of DI water. The pH was adjusted to 8 using NaOH pellets, to dissolve the EDTA.
Depletion interaction
We controlled the attraction between colloids using depletion interactions with PEO polymer as the depletant. Unless otherwise stated, in each experiment, we added a stock solution of PEO to a concentration of 3.25 g l−1. Following the derivation given in ref. 47, we estimated the strength of the induced attraction using the following equation:
where r is the radius of the depletant (57 nm), ndep is the number density of the depletant, R is the radius of the colloids (1.1 μm) and h is the distance between the colloids. Setting h = 0, we estimated the maximum strength of the attraction to be ΔE = −75 kBT. Additionally, we understand the strength of the attraction to be linearly proportional to the concentration of the depletant, with an interaction range proportional to the radius of the depletant.
Microscopy
All data were captured with a Nikon TI Eclipse microscope equipped a motorized stage, controlled using the Micro-Manager software. The microscope had two cameras. A Hamamatsu Orca Flash 4.0 CMOS, using a 2,048 × 2,048 pixel field of view and 16 bits, was used for taking time-lapse photographs with a frame rate of 1 frame per minute. For videos requiring a faster frame rate, we used an Edmunds Optics USB 3.0 CMOS camera, with a 480 × 752 pixel field of view and 8 bits. All images were captured with a Nikon ×20 objective (numerical aperture = 0.45) using bright-field microscopy, unless otherwise stated.
Computational model
A colloid is composed of a central core of diameter σ, interacting with other cores via an attractive (20 kBT) cosine-squared potential of range 1.15σ and a repulsive Weeks–Chandler–Anderson potential for distances < σ. The surface of each core is decorated with 5 rigid patches, as in ref. 48, which interact with other patches with a weakly repulsive (10 kBT) cosine-squared potential of diameter 0.15σ, thereby producing friction and transmitting rotation. Molecular dynamics simulations are performed using a Langevin thermostat with a characteristic time of ten time steps, so that motion is practically Brownian when no internal torque is applied. Simulations are performed in LAMMPS49 and visualized with OVITO50. Details in the Supplementary Information.
Reporting summary
Further information on the research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
The data that support the plots within this paper and other findings of this study are available from the corresponding author upon request.
Code availability
The code that supports the plots within this paper and other findings of this study are available from the corresponding author upon request.
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Acknowledgements
D.G. and J.P. thank E. Krasnopeeva, C. Guet, G. Guessous and T. Hwa for providing the E. coli strains. This material is based upon work supported by the US Department of Energy under award DE-SC0019769. I.P. acknowledges funding by the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie Grant Agreement No. 101034413. A.Š. acknowledges funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (Grant No. 802960). M.C.U. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie Grant Agreement No. 754411.
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D.G. and J.P. conceived of the work and designed the experiment. D.G. performed the experimental research and analysed experimental data. I.P., A.Š., D.G. and J.P. designed the simulations. I.P. performed the simulations and analysed the data from the simulations. D.G., M.C.U., J.P. and E.H. performed the analytical modelling and related comparisons with experiments. D.G. and J.P. wrote the paper. D.G., I.P. and M.C.U. wrote the Supplementary Information. All authors reviewed and commented on the paper and on the Supplementary Information.
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Supplementary information
Supplementary Information
Description of videos, Supplementary text and Figs. 1–15.
Supplementary Video 1
(Experiment) Comparison of aggregation in thermal versus active baths. Sticky colloids aggregate in either a thermal (ρB = 0) or active bath of swimming E. coli (ρB = 0.5 ρ*). All other conditions were kept fixed (concentration of colloids, strength of attraction by depletion, objective and speed of acquisition). The field of view in each video is 650 × 650 μm. The dynamics appear much faster in the active bath than in the thermal bath, and the morphology of the aggregates differ. Real time for the video is 4 h.
Supplementary Video 2
(Experiment) Persistent clockwise rotation of aggregates in the active bath. An accelerated video of sticky colloids in an active bath of swimming E. coli (ρB = ρ*), which highlights the persistent clockwise motion of the aggregates. Real time for the video is 2 h.
Supplementary Video 3
(Simulation) Conventional aggregation in a thermal bath. Simulated aggregation of colloids with no internal activity. The internal torque for each particle was set to 0 kBT; see Supplementary Information. The playback speed increases twice during the video, at times 10,000 and 50,000, to capture both the formation of small aggregates at short times and the formation of large structures and gelification on longer timescales. The time of each frame is indicated in the top right corner. Packing fraction Φ = 0.16.
Supplementary Video 4
(Simulation) Aggregation at low activity. The internal torque for each particle was set to 1 kBT; see Supplementary Information. Rotation and partial folding of the aggregates arise, resulting in faster and morphologically different aggregation, compared to the thermal case. The system percolates and forms a space-spanning network at large time. The playback speed increases twice during the video, at times 10,000 and 50,000, to capture both the formation of small aggregates at short times and the formation of large structures and gelification on longer timescales. The time of each frame is indicated in the top right corner. Packing fraction Φ = 0.16.
Supplementary Video 5
(Simulation) Aggregation at high activity. The internal torque for each particle was set to 10 kBT; see Supplementary Information. Rotation and folding of the aggregates arise, resulting in faster and morphologically different aggregation, compared to the thermal case. The system does not percolate and the gel phase is replaced by compact clusters. The playback speed increases twice during the video, at times 10,000 and 50,000, to capture both the formation of small aggregates at short times and the dynamics on long-time scales. The time of each frame is indicated in the top right corner. Packing fraction Φ = 0.16.
Supplementary Video 6
(Simulation) Zoomed in view of aggregation and folding at high activity. This is a subsection of the simulation window, focusing on the non-conventional aggregation of particles. The rotation and folding lead to the formation of a compact cluster. The internal torque for each particle was set to 10 kBT; see Supplementary Information. The playback speed is constant, and the time of each frame is indicated in the top right corner. Packing fraction Φ = 0.16.
Supplementary Video 7
(Simulation) Mechanical test of a gel assembled in thermal conditions. The gel was previously assembled with the internal torque for each particle set to 0 kBT (see Supplementary Information). The internal torque remained at 0 kBT during the stretching experiment. The strain increases with time, and the exact value is indicated in the top right. The gel exhibits an elastic response in the considered stain regime (see the main text).
Supplementary Video 8
(Simulation) Mechanical test of a gel assembled in active conditions. The gel was previously assembled in active conditions. The internal torque for each particle was set to 3 kBT; see Supplementary Information. The internal torque was set to 0 kBT during the stretching experiment. The strain increases with time, and the exact value is indicated in the top right. The aggregation in active conditions results in a gel with unconventional structure, compared with the gel assembled in thermal conditions (Supplementary Video 7). This results in a nonlinear mechanical response, akin to a mechanical diode.
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Grober, D., Palaia, I., Uçar, M.C. et al. Unconventional colloidal aggregation in chiral bacterial baths. Nat. Phys. 19, 1680–1688 (2023). https://doi.org/10.1038/s41567-023-02136-x
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DOI: https://doi.org/10.1038/s41567-023-02136-x
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