Site-specific modification of RNA in cells is crucial for analysis and functional investigations. Natural enzymes that promote RNA methylation using S-adenosyl-l-methionine (SAM) exist, but leveraging these proteins for RNA modification is limited by cell permeability, stability and specificity of their substrates. Now, a de novo ribozyme that acts on a stabilized and cell-permeable SAM analogue enables site-specific RNA modification with a click handle in living cells.
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Cornelissen, N.V., Rentmeister, A. Ribozyme for stabilized SAM analogue modifies RNA in cells. Nat. Chem. 15, 1486–1487 (2023). https://doi.org/10.1038/s41557-023-01354-3
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DOI: https://doi.org/10.1038/s41557-023-01354-3
