Tailor-made reverse transcriptases are used in molecular biology and synthetic genetics. However, re-engineering these enzymes to work with non-natural nucleic acids is difficult and requires powerful directed evolution strategies. Now, an adaptable selection approach has been demonstrated for the evolution of new reverse transcriptases.
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Henkel, M., Marx, A. Expanding the reverse transcription toolbox. Nat. Chem. 12, 668–669 (2020). https://doi.org/10.1038/s41557-020-0501-9
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DOI: https://doi.org/10.1038/s41557-020-0501-9
