A new pyrrolysyl-tRNA synthetase/PyltRNA (PylRS/PyltRNA) pair that is mutually orthogonal to existing PylRS/PyltRNA pairs has now been discovered and optimized. This system could enable the site-specific incorporation of a greater number of distinct non-canonical amino acids into a protein.
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Ngai, W.S.C., Chen, P.R. Optimizing orthogonality. Nature Chem 10, 802–803 (2018). https://doi.org/10.1038/s41557-018-0115-7
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DOI: https://doi.org/10.1038/s41557-018-0115-7