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Thermal denaturation and renaturation of a double-helical polysaccharide xanthan in acidic and basic solutions

Polymer Journal (2024)Cite this article

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Abstract

The double helix of a polysaccharide, xanthan was unwound by heating (denaturation) and rewound by cooling (renaturation) in acidic (0.01 M HCl) and basic (0.01 M NaOH) solutions. Circular dichroism spectra of xanthan renatured in the acidic and basic solutions were similar to those of native xanthan, which suggests that the local helical structures of the renatured xanthan were recovered by renaturation. The changes in the molar mass and intrinsic viscosity of xanthan induced by renaturation in the acidic solution can be explained by the renaturation scheme in the neutral solution, as reported previously. (Matsuda et al. Polym. J. 41, 526-532, 2009). Both the molar mass and radius of gyration of xanthan in the basic solution were decreased by denaturation and renaturation, and the molar mass dependence of the radius of gyration was similar to that of native xanthan. This change can be explained by the formation of a hairpin structure during renaturation in the basic solutions.

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References

  1. Norisuye T, Teramoto A. In Polymeric Materials Encyclopedia (ed. Salamone, JC) 8801-9 (RC Press, Boca Raton, USA, 1996).

  2. Sato T, Norisuye T, Fujita H. Double-stranded helix of xanthan in dilute solution: evidence from light scattering. Polym J. 1984;16:341–50.

    Article  CAS  Google Scholar 

  3. Sato T, Kojima S, Norisuye T, Fujita H. Double-stranded Helix of Xanthan in dilute solution: further evidence. Polym J. 1984;16:423–9.

    Article  CAS  Google Scholar 

  4. Sato T, Norisuye T, Fujita H. Double-stranded Helix of Xanthan in dilute solution: dimensional and hydrodynamic properties in 0.1 M aqueous sodium chloride. Macromolecules. 1984;17:2696–2700.

    Article  ADS  CAS  Google Scholar 

  5. Liu W, Sato T, Norisuye T, Fujita H. Thermally induced conformational change of Xanthan in 0.01 M aqueous sodium chloride. Carbohyd Res. 1987;160:267–81.

    Article  CAS  Google Scholar 

  6. Liu W, Norisuye T. Order-disorder conformation change of Xanthan in 0.01 M aqueous sodium chloride: dimensional behavior. Biopolymers. 1988;27:1641–54.

    Article  CAS  Google Scholar 

  7. Capron G, Brigand G, Muller G. About the native and renatured conformation of xanthan exopolysaccharide. Polymer. 1997;38:5289–95.

    Article  CAS  Google Scholar 

  8. Oviatt HW Jr, Brant DA. Viscoelastic behavior of thermally treated aqueous Xanthan solutions in the semidilute concentration regime. Macromolecules. 1994;27:2402–8.

    Article  ADS  CAS  Google Scholar 

  9. Kawakami K, Okabe Y, Norisuye T. Dissociation of dimerized xanthan in aqueous solution. Carbohydr Polym. 1990;14:189–203.

    Article  CAS  Google Scholar 

  10. Matsuda Y, Bjiyajima Y, Sato T. Thermal denaturation, renaturation, and aggregation of a double-helical polysaccharide Xanthan in aqueous solutions. Polym J. 2009;41:526–32.

    Article  CAS  Google Scholar 

  11. Matsuda Y, Sugiura F, Mays JW, Tasaka S. Atomic force microscopy of thermally renatured Xanthan with low molar mass. Polym J. 2015;47:282–5.

    Article  CAS  Google Scholar 

  12. Matsuda Y, Sugiura F, Okumura KW, Tasaka S. Renaturation behavior of Xanthan with high molar mass and wide molar mass distribution. Polym J. 2016;48:653–8.

    Article  CAS  Google Scholar 

  13. Matsuda Y, Okumura KW, Tasaka S. Molar mass dependence of structure of Xanthan thermally denatured and renatured in dilute solution. Polym J. 2018;50:1043–9.

    Article  CAS  Google Scholar 

  14. Zhang L, Liu W, Norisuye T, Fujita H. Double-stranded helix of xanthan: Rigidity in 0.01M aqueous sodium chloride containing 0.01 N hydrochloric acid. Biopolymers. 1987;26:333–41.

    Article  CAS  Google Scholar 

  15. Causse B, Spadini L, Sarret G, Faure A, Travelet C, Madern D, et al. Xanthan exopolysaccharide: Cu2+ complexes affected from the ph-dependent conformational state; implications for environmentally relevant biopolymers. Envir Sci Tech 2016;50:3477–85.

    Article  CAS  Google Scholar 

  16. Matsuda Y, Ashizawa H, Fukui T, Akao R, Tasaka S. Fabrication of polymer structure among fibrous structure of poly(lactic acid) gel and improvement of physical properties. Macromol Chem Phys. 2018;219:1700317.

    Article  Google Scholar 

  17. Sato T, Norisuye T, Fujita H. Triple helix of Schizophyllum commune polysaccharide in dilute solution. 5. Light scattering and refractometry in mixtures of water and dimethyl sulfoxide. Macromolecules. 1983;16:185–9.

    Article  ADS  CAS  Google Scholar 

  18. Tomofuji F, Matsuo K, Terao K. Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride. Carbohydr Polym. 2022;275:118681.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was partially supported by MEXT Promotion of Distinctive Joint Research Center Program Grant Number# JPMXP 0621467946. The analysis of the samples was partly carried out with a JASCO J-720KS at the Instrumentation Center, The University of Kitakyushu. We thank Professor Takahiro Sato at the Open University of Japan who kindly provided the xanthan samples and the dialyzer and, allowed us to use the CD instrument. This work was supported by JSPS KAKENHI Grant Number 19H02773.

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Authors and Affiliations

  1. Department of Applied Chemistry and Biochemical Engineering, Shizuoka University, 3-5-1 Johoku, Chuo-ku, Hamamatsu, 432-8561, Japan

    Yasuhiro Matsuda, Ryoga Saiki, Kaede Sato & Atsuya Kashiwagi

  2. Division of Molecular Science, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan

    Kazuto Yoshiba

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  1. Yasuhiro Matsuda
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  2. Ryoga Saiki
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Correspondence to Yasuhiro Matsuda.

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Matsuda, Y., Saiki, R., Sato, K. et al. Thermal denaturation and renaturation of a double-helical polysaccharide xanthan in acidic and basic solutions. Polym J (2024). https://doi.org/10.1038/s41428-024-00892-y

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  • DOI: https://doi.org/10.1038/s41428-024-00892-y

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