Abstract
Cerebrovascular pressure autoregulation (CPA) regulates cerebral blood flow (CBF) in relation to changes in mean arterial blood pressure (MAP). Identification of a pressure-passive cerebral perfusion and the potentially modifiable physiologic factors underlying it has been difficult to achieve in sick infants. We previously validated the near-infrared spectroscopy–derived hemoglobin difference (HbD) signal (cerebral oxyhemoglobin − deoxyhemoglobin) as a reliable measure of changes in CBF in animal models. We now sought to determine whether continuous measurements of ΔHbD would correlate to middle cerebral artery flow velocity (CBFV), allow identification and quantification of pressure-passive state, and help to delineate potentially modifiable factors. We enrolled 43 infants (2 d to 7 mo old) who were undergoing open cardiac surgery and cardiopulmonary bypass. At 6 and 20 h after surgery, we measured changes in HbD, CBFV (by transcranial Doppler), and MAP at different end-tidal CO2 levels. We assigned a pressure-passive index (PPI) to each study on the basis of the relative duration of significant coherence between ΔMAP and ΔHbD. We found a significant relationship between ΔHbD and ΔCBFV at both time points. At 6 h after surgery, we showed high concordance (coherence >0.5; PPI ≥41%) between ΔMAP and ΔHbD, consistent with disturbed CPA in 13% of infants. End-tidal CO2 values ≥40 mm Hg and higher MAP variability both were associated with increased odds (p < 0.001) of autoregulatory failure. This approach provides a means to identify and quantify disturbances of CPA. High CO2 levels and fluctuating MAP are two important preventable factors associated with disturbed CPA.
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Main
Recent advances in infant cardiac surgery and critical care medicine have translated into high survival rates for infants with congenital heart disease. As mortality has decreased, the clinical focus has shifted to the prevention of perioperative neurologic injury (1). Previous studies have focused primarily on intraoperative cerebral hypoxia-ischemia as a key mechanism for neurologic dysfunction. However, recently it has become clear that multiple potential mechanisms, operating at different times, may be implicated in the pathogenesis of neurologic dysfunction after infant cardiac surgery (2). In the current research, we focused on the early postoperative period because of its high risk for hemodynamic instability and therefore potential for brain injury.
A complex system of intrinsic cerebral vasoregulatory mechanisms modulates cerebral blood flow (CBF) to maintain adequate oxygen and nutrients for normal brain metabolism. Previous studies by our group demonstrated an impairment in one component of intrinsic cerebral vasoregulation, CO2 vasoreactivity, during the early postoperative period (3). Cerebrovascular pressure autoregulation (CPA) maintains CBF constant over a range of cerebral perfusion pressures (the “autoregulatory plateau”) (4). When CPA fails, changes in blood pressure are associated with parallel changes in CBF, i.e. the cerebral circulation becomes pressure passive.
Despite the potential for hemodynamic instability in the early period after infant cardiac surgery, CPA has not been adequately studied in this setting. This deficiency is due in part to the lack of techniques for the noninvasive and continuous study of cerebral perfusion in infants during this period of intensive care. Previous studies have evaluated CPA with transcranial Doppler (TCD) to measure CBF velocity. However, the TCD technique has several potential limitations, including the difficulty during prolonged studies of maintaining a constant and reproducible insonation angle to the artery of interest. Moreover, this technique quantifies velocity of blood flow but not volume of blood flow. Near-infrared spectroscopy (NIRS) provides another approach for studying cerebral hemodynamics and oxygenation at the bedside of the sick infant. In previous validation studies in animals, we demonstrated a strong correlation between changes in the NIRS measurement of hemoglobin difference (HbD; i.e. the difference between cerebral oxyhemoglobin and deoxyhemoglobin concentrations) and CBF as measured by the gold standard radioactive microsphere technique (5,6). Subsequently, we studied CPA in premature infants by measuring the relationship between HbD and mean arterial blood pressure (MAP) and identified a pressure-passive state in 53% of those studied (7).
In the current research, our overall aims were to compare measurements of cerebral perfusion made by TCD and NIRS, to define the integrity of CPA in infants after open-heart surgery, and to determine factors associated with a disturbance in CPA in this setting. We hypothesized that NIRS parameters, specifically HbD, would correlate with CBF velocity by TCD; pressure-passive cerebral perfusion would be identifiable by simultaneous measurements of HbD and MAP; and higher CO2 levels would be associated with pressure-passive cerebral perfusion.
METHODS
Patient enrollment.
Infants were enrolled consecutively between July 2001 and August 2002 at Children's Hospital Boston. Eligibility criteria included infants who were ≤9 mo of age, had congenital heart disease, underwent corrective cardiac surgery using deep hypothermia and cardiopulmonary bypass, and had a birth weight >2.25 kg. The exclusion criteria were known dysmorphic or genetic syndrome, known neurologic abnormality before surgery, and previous cardiac surgery. The study was approved by the Institutional Review Board of Children's Hospital Boston, and written informed parental consent was obtained before surgery. A standardized protocol of cardiopulmonary bypass and anesthesia methods was used during the cardiac operation (8).
Physiologic measurements.
We measured cerebral oxygenation and hemodynamics with NIRS (NIRO 500; Hamamatsu Photonics, Hamamatsu City, Japan) a well-described, noninvasive technique for studying cerebral hemodynamics at the bedside of infants who require critical care (9,10). On the basis of modification of the Beer-Lambert law, determination of changes in light absorbance in the brain allows the calculation of changes in concentration (in micromoles per liter) of the chromophores oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), and oxidized cytochrome aa3 (CytO2). The HbD is derived by subtracting Hb from HbO2, whereas their summation results in Hb total (HbT).
We used transcranial Doppler (TCD; SN: KSO10064; Nicolet Biomedical, Madison, WI) to acquire mean middle cerebral artery timed averaged flow velocity (CBFV) over a period of 5 s. We also obtained mean middle cerebral artery resistive indices [RI; (systolic FV − diastolic FV)/systolic FV], a measure of cerebral vascular resistance. We positioned the TCD probe beside the NIRS optodes on the temporal acoustic window and fixed it with an elastic band for insonation at designated times.
We measured systemic parameters, including MAP, from an indwelling arterial catheter via a pressure transducer (Hewlett Packard cardiac monitor M1047A), arterial oxygen saturation (Sao2) by pulse oximetry (Nellcor N100, Hayward, CA), and end tidal CO2 (ET-CO2) by end tidal capnometer (Novometrix Capnogard, Wallingford, CT).
Study design.
We aimed to perform cerebral and systemic measurements at two time points in the postoperative period; 6 h (“early sessions”) and 20 h (“late sessions”) after the onset of intraoperative rewarming from hypothermia. We commenced each study only after the approval of the cardiac intensive care unit (ICU) attending physician. Continuously and simultaneously, we recorded all NIRS parameters and systemic measurements (MAP, Sao2, and ET-CO2). All recorded data were sampled at a frequency of 2 Hz, displayed and stored in a laptop computer in a time-locked manner.
A period of at least 10 min of stable CO2 (as monitored by ET-CO2) and HbD (as monitored by NIRS) was recorded. We then induced measured changes in CO2 by adjusting the ventilator rate while maintaining constant ventilatory pressure, inspired O2 concentrations, and Sao2. We then allowed sufficient time for CO2 and HbD to stabilize at the new CO2 level as assessed by visual inspection of the on-line screen display. ET-CO2 measurements were adjusted to arterial CO2 measurements at the beginning of each study session. Before and after a ventilator change, we measured CBFV and RI by TCD.
Data processing.
We then processed the cerebral and systemic data in the following manner. We visually screened for movement artifacts and eliminated these data to ensure high quality of our measurements. Because NIRS measurements are influenced by systemic Sao2 changes, our measurements included only periods of stable Sao2 with Sao2 fluctuations <5%.
We then obtained the 1-min average for all systemic and cerebral measurements at two time points, before a ventilator change and after the ET-CO2, HbD, and CBFV signals had stabilized at their new level. Change in these variables was determined by comparing the 1-min averaged values before and after the ventilator rate change. The difference between the measurements was presented as “Δ values” and used for correlations between changes in TCD and NIRS variables.
We studied CPA by evaluating the correlation between continuous measurements of MAP with simultaneous measurements of cerebral HbD, as an index of CBF. We divided the study data into consecutive and continuous 5-min epochs. We then subjected each 5-min epoch to a uniform and consistent computation. CPA is believed to be frequency specific (11,12). Therefore, we analyzed the relationship between MAP and HbD in a frequency-specific manner, using transfer function analysis in the frequency domain. Transfer function analysis is an established approach for analyzing the relationship between complex and changing physiologic signals (7,11,12) that uses Fourier transformation to compute the statistical correlation of two waveforms (in our study, MAP and HbD). In addition, it is known that autonomic control of the circulation occurs at low frequencies (13). We therefore used detection frequencies of 0–0.1 Hz, a bandwidth that corresponds to changes that occur over several minutes and hence of physiologic importance for autoregulatory control (12).
For minimizing the risk of aliasing, the upper bandwidth detection limit (1 Hz) was half of the sampling rate (2 Hz, or a sampling interval of 0.5 s), thereby meeting the requirements of the Nyquist theorem. For each 5-min data epoch, the degree of correlation between the MAP and HbD waveforms, at a given frequency range, can be quantified as a coherence score. A coherence score ranges between 0 (no correlation) and 1.0 (perfect correlation). By convention, coherence scores >0.5 were considered to represent significant concordance between the waveforms of MAP and HbD and, in our model, to indicate disturbed pressure autoregulation. Thus, each 5-min epoch could be categorized as either coherent (coherence score between 0.5 and 1, i.e. pressure passive) or noncoherent (coherence score between 0 and 0.5, i.e. pressure autoregulating). We then assigned an overall pressure-passive index (PPI) to each study session by calculating the percentage time of pressure passivity (i.e. number of coherent epochs/total number of epochs in that study session). A PPI of 0 indicates perfect autoregulation, whereas a PPI of 100% suggests a completely pressure-passive cerebral circulation over the entire study session. Computations were performed by a Matlab software package (Matlab, Natick, MA).
Next we combined the 5-min data epochs of all patients in the study and divided these into two dichotomous groups according to their MAP and HbD relationship—coherent (pressure passive) epochs versus noncoherent (pressure autoregulating) epochs—for further specific physiologic correlations. We documented the available mean ET-CO2 and MAP values of each time epoch. Because normal MAP differs with age, we used published normative values for each patient according to his or her age at surgery (14). Each MAP value was categorized as normal, elevated (>1 SD or >2 SD above the mean), or decreased (<1 SD or 2 SD below the mean). We also calculated the MAP coefficient of variation (CV), defined as the SD divided by the mean, for each 5-min epoch as a measure of MAP instability.
Clinical data.
To examine the effect of various clinical factors on CPA, we systematically reviewed the patient's medical record. We considered the following as potential risk factors during the pre- and intraoperative periods for subsequent CPA disturbance: cyanotic congenital heart disease (transposition of great arteries and tetralogy of Fallot), preoperative intubation (as a measure of respiratory instability), deep hypothermic circulatory arrest duration >20 min, and minimum intraoperative rectal temperature <18°C (i.e. “deep” hypothermia). We considered the effect on CPA of individual risk factors as well as the effect of the sum of risk factors used as a gross index of morbidity.
In addition, we considered the effect on CPA of specific conditions during each study session: time since intraoperative rewarming, core (rectal) temperature, acidosis (any blood pH <7.25 on day of study), and the medications in use. We reviewed and documented all potentially vasoactive medications used during the course of the study period. These were grouped into the following classes: inotropic agents (digoxin, dobutamine, dopamine), paralyzing agents (pancuronium, rocuronium, cisatracurium), sedative-analgesic agents (fentanyl, morphine, chloral hydrate, midazolam, ketamine), and antihypertensive diuretic agents (spironolactone, captopril, alprostadiol, furosemide). We considered the effect on CPA of individual drug classes (as grouped above), as well as the effect of each drug used.
Statistical analysis.
Continuous measurements are expressed as mean ± SD. The Pearson correlation coefficient (r) was used to measure the relationship between changes in Doppler measurements and changes in NIRS parameters. Generalized estimating equation models, which account for the correlation among multiple measurements made on the same patient, were used to calculate p values. Correlations were estimated for early and late studies separately and for all studies combined. The relationship between patient PPIs at the early and late studies was evaluated using the Spearman rank correlation coefficient (rs). Comparisons of mean CO2 levels and percentages of epochs with abnormal MAP were performed for pressure-passive versus autoregulating 5-min epochs using generalized estimating equation models to account for the correlation among multiple epochs for the same patient; these models were also used to estimate odds ratios (OR). For each patient, relationships between categories of PPI and potential risk factors were evaluated using Fisher's exact test.
RESULTS
We studied 43 infants (17 girls), aged 2 d to 7 mo (median 9 d), who overall had a total of 75 study sessions. After the onset of rewarming, early study sessions occurred after a mean of 5.7 h (±1.1), and late study sessions occurred after a mean of 19.8 h (±2.9). Study sessions lasted 52 min (±1.5) and 53 min (±1.2) for early and late studies, respectively. Thirty-two (75%) infants had both early and late study sessions, whereas 7 had only early study sessions and 4 had only late study sessions. Twenty-one (48.8%) infants had transposition of the great arteries, 13 (30.2%) had tetralogy of Fallot, and 9 (20.9%) had ventricular septal defect. Twenty-one (48.8%) infants were intubated before admission to surgery, 1 (2.3%) infant had an Apgar score <5 at 5 min, and 1 (2.3%) infant was acidotic on a study day. Median rectal temperatures during the early and late study sessions were 36.5°C (33.7–38) and 36.5°C (35.5–38.8), respectively. The clinical and demographic data of these infants are presented in Table 1. Clinical characteristics of infants with measurements at a single time point did not differ from those with measurements at both early and late sessions.
Relationship between NIRS parameters and Doppler measurements.
We evaluated the correlations between ΔCBFV, ΔRI, and ΔNIRS measurements. We used data from 32 early and 20 late study sessions that had NIRS and TCD data of adequate quality. We excluded TCD and NIRS data from 7 early and 16 late study sessions because of artifact-contaminated tracings (e.g. movement artifacts, baseline shift) or inability to induce CO2 changes because infants had recovered spontaneous respiration. We found no significant clinical difference between patients with excluded study sessions and those with adequate study sessions. Data are presented in Table 2. Changes in ET-CO2 had no significant effect on MAP or arterial Sao2 (r = −0.08, p = NS; and r = 0.02, p = NS, respectively). We found a significant relationship between ΔCBFV and ΔHbD in both the early and the late studies and in all studies combined (r = 0.64, p < 0.001; Fig. 1). A similar relationship was found between ΔHBO2 and ΔCBFV (r = 0.59, p < 0.001), whereas the relationship between ΔHbT and ΔCBFV was marginal (r = 0.32, p = 0.04). The relationship between ΔHb and ΔCBFV was not significant. Furthermore, ΔRI showed possible correlations with ΔHbD, ΔHBO2, and ΔHbT (r = −0.37, p = 0.006; r = −0.41, p < 0.001; and r = −0.32, p = 0.004, respectively) but not with ΔHb. We also found significant relationships between ΔCO2 and ΔHbD and between ΔCBFV and ΔHbD, ΔCBFV, and ΔRI (r = 0.74, p < 0.001; r = 0.64, p < 0.001; and r = −0.64, p < 0.001, respectively).
Disturbed CPA at early and late studies.
Because the cutoff for defining abnormal PPI is unknown, we arbitrarily divided the infants into two groups: intact CPA, i.e. infants who had no concordant relationship between MAP and HbD (coherence <0.5 in all study measurements; PPI = 0), and disturbed CPA, i.e. infants who had periods of a concordant relationship between MAP and HbD (coherence >0.5; PPI = 1–100%). Figure 2 shows an example of a patient with disturbed CPA. According to our model, CPA in most infants was either intact or mildly disturbed (PPI 1–20%). At 6 h after surgery, we found high concordance (coherence >0.5; PPI ≥41%) between MAP and HbD in 5 (13%) infants, consistent with disturbed CPA. Table 3 shows the distribution of PPI for early and late studies separately. For the 32 patients who had both early and late studies, we found a moderate correlation between PPI at each time point (rs = 0.37, p = 0.03). All infants with disturbed CPA (coherence >0.5; PPI ≥41%) at 18 h had been pressure passive at 6 h. This finding suggests that infants with disturbed CPA during the early postoperative period were likely to remain in a pressure-passive state of CPA into the late postoperative period (Fig. 3).
Data analysis at the epoch level. Acquiring all NIRS and systemic hemodynamic measurements in a continuous time-locked manner allowed an overall data analysis across all epochs. We analyzed data for early and late studies separately because these periods might have different hemodynamic characteristics. Across all 39 patients who had early studies, a total of 359 5-min data epochs were collected. Fifty-three (14.8%) epochs showed a pressure-passive relationship (i.e. coherence >0.5) between MAP and HbD, whereas 306 showed an autoregulating relationship (i.e. coherence <0.5). Across the 36 patients who had late studies, a total of 313 epochs were measured, 43 (13.7%) of which showed a pressure-passive relationship and 270 of which showed an autoregulating relationship between MAP and HbD. We used the total sets of 5-min data epochs to examine the relation between CPA and ET-CO2 as well as the effect of mean MAP and its variance over each 5-min epoch.
Association between ET-CO2 levels and CPA.
ET-CO2 values were available for 348 of 359 early study epochs. Mean ET-CO2 was 40.9 ± 5.5 for pressure-passive epochs and 35.9 ± 5.9 for autoregulating epochs (p < 0.001); thus, a higher ET-CO2 was associated with a pressure-passive versus an autoregulating epoch. Having ET-CO2 value ≥40 increased the odds of an epoch's being pressure passive compared with ET-CO2 <40 [OR 5.2; 95% confidence interval (CI) 2.4–10.9; p < 0.001; Table 4].
ET-CO2 measurements were available for 292 of 313 late study epochs. Mean ET-CO2 was 41.0 ± 6.3 for pressure-passive epochs and 39.0 ± 5.2 for autoregulating epochs (p = 0.04). However, the odds of an epoch's being pressure passive with ET-CO2 value ≥40 were not statistically significant (OR 1.7; 95% CI 0.8–3.6; p = 0.15). The association between hypercapnea and disturbed autoregulation thus is more conspicuous during early studies.
Association between MAP and CPA.
Considering all of the 5-min epochs across all studies, we found that 200 (56%) early study epochs and 217 (69%) late study epochs had mean MAP values that were considered “normal” for age (see definition in “Methods”). A total of 137 (38%) early study epochs and 92 (29%) late study epochs had mean MAP values between 1 and 2 SD above or below the mean. Only 21 (6%) epochs of early studies and 3 (1%) epochs of late studies had MAP values at least 2 SD either above or below the expected normal for age. Although a larger percentage of early study epochs was either low or high, the vast majority of epochs had normal or near-normal mean MAP values. No significant relationship was found between either low MAP (<1 SD or <2 SD below the mean) or high MAP (>1 SD or >2 SD above the mean) and an epoch's being pressure passive or autoregulating.
We also calculated the MAP CV of each epoch (see “Methods”). For early studies, MAP CV ≥0.08 increased the odds of an epoch's being pressure passive (OR 4.7; 95% CI 2.1–10.5; p < 0.001). For late studies, MAP CV ≥0.08 again increased the odds of an epoch's being coherent (OR 2.8; 95% CI 1.6–5.0; p < 0.001).
Association among perioperative risk factors, medication use, and CPA.
No significant associations were found between CPA and perioperative risk factors either individually or as a sum of classes. No significant associations were found between CPA and either individual drugs or drug classes (see “Methods” for detailed list of risk factors and drugs).
DISCUSSION
In this prospective study of infants after cardiac surgery, we first demonstrated a significant correlation between the NIRS-derived parameter of HbD and CBFV measured by TCD. Next we demonstrated the feasibility of using continuous HbD measurements to evaluate CPA during the early critical care period in these infants. Finally, we demonstrated that CPA may be disturbed in the early postoperative period, particularly at higher CO2 tensions and during periods of fluctuating systemic blood pressure. To our knowledge, the current study is the first to use NIRS measurements and coherence analysis to study CPA in infants after open-heart surgery.
A major impediment to the study of cerebral vasoregulation at the bedside of critically ill infants is the limited availability of safe and reliable techniques for making continuous measurements of cerebral perfusion. Clinical studies have demonstrated the feasibility of using NIRS for measuring cerebral vasoreactivity in critical conditions ranging from deep hypothermic infant cardiac surgery (3) to sick term and preterm infants (15,16). We used in the current study the NIRS-derived HbD as a measure of CBF, having previously validated this technique in two neonatal piglet models using radioactive microspheres (5,6).
Of interest in our current study is the finding of a significant correlation between measurements of ΔHbD and measurements of ΔCBFV and ΔRI by TCD. There is a long-standing controversy about the ability of TCD to accurately reflect volemic CBF (17–19). TCD measures flow velocity in large vessels and makes inferences about “downstream” vascular changes on the assumption that the vessel diameter at the point of insonation remains constant. However, reactivity and diameter changes have been demonstrated in the fetus and newborn (20,21), bringing into question the validity of the TCD technique. Our findings and those of others (22,23) suggest a correlation between measurements made by NIRS and TCD.
To date, the integrity of CPA has been studied mainly through the use of a so-called static approach, which examines the steady-state relationship between CBF and blood pressure at distinct time points (24–28). CPA is known to be a dynamic system that is influenced by multiple factors (e.g. CO2 tension, hypoxia, drugs) with different mechanisms operating at different rates (29). Our data suggest that CPA is not an “all-or-none” phenomenon but rather a system with fluctuating (dynamic) characteristics. Unlike the static approach, a dynamic approach using a frequency-dependent analysis (e.g. coherence function analysis) is capable of capturing and describing an enormous wealth of CPA data in the time domain. Previous studies have used such a dynamic approach to study CPA using TCD measurements of CBF velocity (12,30–33). Our group previously used coherence function analysis in premature infants and demonstrated that concordant changes between MAP and HbD (“pressure passive” state) were significantly associated with cerebrovascular lesions (7).
In our study, each 5-min epoch of data was assigned a coherence score; by convention, a coherence score between two signals of 0.5 or above indicates significant concordance between the two signals at that frequency. In our study, 5-min epochs with coherence between HbD and MAP of 0.5 or above were considered pressure passive. Next we used an index that quantifies disturbed CPA according to its duration over the course of a study, i.e. the percentage of all 5-min epochs that were pressure passive. The relationship between disturbed CPA and subsequent brain injury would be expected to depend on the magnitude of disturbances in blood pressure imposed on the cerebral autoregulatory system. Longer periods of concordance between MAP and HbD may be capable of identifying infants who have disturbed CPA and are at greater risk for brain injury should periods of hemodynamic instability develop. In the current study, most but not all infants had intact or mildly disturbed CPA. When present, a disturbance in CPA was usually evident early in the postoperative course and persisted through the first 20 h in most cases. The relatively low occurrence of postoperative pressure-passive cerebral circulation in our study may reflect the quality of intraoperative and postoperative treatment of these infants. We recently published data showing a dramatic decrease in the incidence of postoperative neurologic dysfunction at our center (34).
The cerebral pressure autoregulatory system responds to decreases in blood pressure by relaxing the vascular smooth muscle tone, causing vasodilation, thereby maintaining CBF. With a baseline increase in diameter of the resistance arterioles, such as occurs with a lower baseline MAP or increasing circulating Pco2, CPA becomes less efficient because of a reduced residual capacity for vasodilation. For this reason, we evaluated the efficiency of CPA in our infants at different levels of mean MAP within the 5-min epochs, as well as at different levels of Pco2 as induced by ventilator rate changes. We found that increased ambient Pco2 levels were associated with increased odds of autoregulatory failure in the early postoperative period. Previous studies in experimental models and in adult humans have shown similar effects of Pco2 on CPA (35–40). Because Pco2 is vasoactive in the pulmonary vasculature, changes in Pco2 are used in certain circumstances to adjust pulmonary vascular resistance in infants after cardiac surgery. Our data suggest that hypercarbia may reduce CPA and render the infant more vulnerable to cerebral injury during periods of blood pressure instability.
In this population of critically ill infants, the administration of pharmacologic agents or tilting of the infant solely for the purpose of inducing experimental blood pressure changes is clearly not feasible. We did not attempt to induce changes in baseline blood pressure purely for the sake of the study and relied entirely on “spontaneous” changes in baseline MAP. We found no statistical relationship between baseline MAP levels and the development of a cerebral pressure-passive state in both the early and the late studies. This finding is likely due in large part to the fact that strict hemodynamic management in our ICU successfully maintained baseline blood pressure within normal limits most of the time. This factor also made it impossible for us to address the upper and lower limits of the autoregulatory plateau in these infants. However, the occurrence of superimposed transient oscillations in MAP allowed us to assess CPA during these otherwise stable periods of baseline MAP. In fact, we found that periods of high MAP variability increased the odds of detecting a pressure-passive cerebral state. A number of previous studies have described intrinsic fluctuations of often unclear origin in cerebral hemodynamics and oxygenation (12,41–46). In one report, disturbances in the mechanics of ventilation induced the fluctuations and allowed formulation of an intervention (muscle paralysis) to correct both the ventilatory abnormalities and the fluctuations (42,43). Discussion of these phenomena is beyond the scope of this report.
This study has several potential limitations. Because our studies could begin only once the patient was deemed clinically stable by the attending, some variability in the timing of our measurements was unavoidable and may have introduced some level of bias. However, this effect is likely to be minor. Because the majority of our infants received their arterial lines and endotracheal tubes only in the operating room, we were not able to study reliably their preoperative CPA. Similarly, our recordings could not continue once infants were extubated and arterial lines were discontinued. Second, we attempted to identify perioperative factors (see “Methods”) that predisposed to disturbance of CPA in these infants. Among the factors that we analyzed, none was predictive of CPA disturbance, a finding that is likely due to the relatively small number of infants with disturbed CPA in our study and the resulting limitation of statistical power. It is also possible that our study population includes infants who were more hemodynamically stable, thus limiting the generalizability of our findings. Moreover, because the treatment of these infants follows strict treatment guidelines, the limited variability of many clinical factors is so small as to limit significantly the statistical power required to identify antecedents of disturbed CPA in this population.
Finally, as stated above, close clinical monitoring and management of blood pressure in these infants limited the range of blood pressures across which CPA could be measured. However, the goal of this study was to demonstrate the feasibility of this technique for studying CPA in these sick infants rather than to define the limits of their cerebral autoregulatory plateau. Furthermore, the presence of disturbed CPA does not necessarily suggest inevitable subsequent neurologic dysfunction but presumably could serve as an indicator that a given infant is at high risk for ischemic cerebral injury should unstable systemic hemodynamics develop. The importance of our findings will require further studies in the ICU and at follow-up.
In summary, we have demonstrated that continuous measurements of changes in HbD correlate well with changes in blood flow velocity measured by the more widely used TCD technique. More important, we also demonstrated the feasibility of monitoring and quantifying CPA in sick infants by analyzing the relationship between continuous changes in HbD and MAP using coherence function analysis. In our population, we found that most but not all infants seemed to have intact CPA during the early postoperative period but that hypercarbia and fluctuating MAP might predispose to a disruption of CPA. These findings have implications for the treatment of these infants in the postoperative period.
Abbreviations
- CBF:
-
cerebral blood flow
- CBFV:
-
cerebral blood flow velocity
- CI:
-
confidence interval
- CPA:
-
cerebrovascular pressure autoregulation
- CV:
-
coefficient of variation
- ET-CO2:
-
end-tidal CO2
- HbD:
-
hemoglobin difference (oxyhemoglobin − deoxyhemoglobin)
- HbT:
-
hemoglobin total (oxyhemoglobin + deoxyhemoglobin)
- ICU:
-
intensive care unit
- MAP:
-
mean arterial blood pressure
- NIRS:
-
near-infrared spectroscopy
- OR:
-
odds ratio
- PPI:
-
pressure-passive index (% time pressure passive state)
- RI:
-
resistive index
- Sao2:
-
arterial oxygen saturation
- TCD:
-
transcranial Doppler
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Acknowledgements
We gratefully acknowledge Dr. Joseph J. Volpe for critical review of our manuscript. We also thank Jody Bartlett and Ellen McGrath for research coordination and Shaye Moore and Michelle Mercadante for help in manuscript preparation. Many thanks to the children and their families for participating in this study.
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This study was supported by a grant from the LifeBridge Fund and grant HL 063411 from the National Heart, Lung, and Blood Institute.
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Bassan, H., Gauvreau, K., Newburger, J. et al. Identification of Pressure Passive Cerebral Perfusion and Its Mediators after Infant Cardiac Surgery. Pediatr Res 57, 35–41 (2005). https://doi.org/10.1203/01.PDR.0000147576.84092.F9
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DOI: https://doi.org/10.1203/01.PDR.0000147576.84092.F9
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