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Cell division

ESCRT'll tear us apart

Nature Reviews Molecular Cell Biology volume 12, pages 208–209 (2011)Cite this article

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The ingression of the cleavage furrow coincides with reorganization of the actin and microtubule cytoskeletons and the plasma membrane. Through detailed live-imaging analysis of abscission, the authors were able to rule out a direct contribution to abscission from some of the usual suspects: vesicle trafficking, microtubules and filamentous actin (F-actin) were not required. Specifically, they saw that EGFP–RAB8 vesicles were no longer present at the intercellular bridge at the point when abscission begins, and disrupting Golgi traffic with brefeldin A had no effect. Furthermore, once the intercellular bridge was formed, disassembly of microtubules by the severing enzyme spastin was needed only for the completion of abscission. Super-resolution fluorescence microscopy also revealed that F-actin was not present at the constriction site and was not required for abscission once the furrow had ingressed.

ESCRT-III... regulates abscission... by promoting the formation of helical filaments that drive cortical constriction.

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References

ORIGINAL RESEARCH PAPER

  1. Guizetti, J. et al. Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science 10 Feb 2011 (doi: 10.1126/science.1201847)

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FURTHER READING

  1. Hurley, J. H. & Hanson, P. I. Membrane budding and scission by the ESCRT machinery: it's all in the neck. Nature Rev. Mol. Cell Biol. 11, 556–566 (2010)

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Authors
  1. Alison Schuldt
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Schuldt, A. ESCRT'll tear us apart. Nat Rev Mol Cell Biol 12, 208–209 (2011). https://doi.org/10.1038/nrm3084

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