Key Points
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Analysis of the single-celled Arabidopsis thaliana trichomes has provided insights into several general cellular processes and machineries, including the function of transcription factors, cell-cycle regulation, the control of the microtubule and actin cytoskeleton, and cell-death control.
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Trichome patterning begins with cells that are initially equivalent. A pattern is generated through cell–cell communication between neighbouring cells, which is mediated by the movement of small MYB-like proteins that interact with a complex that comprises a MYB-like transcription factor, a basic helix–loop–helix (HLH) protein and a WD40 protein.
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Trichomes are polyploid, and overexpression studies and mutants enabled the analysis of how the switch from mitosis to endoreduplication is regulated and how the number of cycles is controlled.
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Trichome branching is controlled by at least four different pathways, which involve microtubules, cell growth or one of two genes — ANGUSTIFOLIA (which is involved in transcriptional regulation or Golgi-dependent processes) or STICHEL (which has unknown biochemical properties).
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The directionality of cell expansion is controlled by the actin-modulating ARP2/3 complex.
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Analysis of these different processes in one model cell type offers the possibility to explore how seemingly unrelated processes might be interconnected during development.
Abstract
During the past few years, the focus in plant developmental biology has shifted from studying the organization of the whole body or individual organs towards the behaviour of the smallest unit of the organism, the single cell. Plant leaf hairs, or trichomes, serve as an excellent model system to study all aspects of plant differentiation at the single-cell level, including the choice of cell fate, developmental control of the cell cycle, cell polarity and the control of cell shape.
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Acknowledgements
I would like to thank H. Meinhardt for providing the images and the movie that are presented in Box 1 and for stimulating discussions. I would also like to thank the members of the laboratory for helpful comments on the manuscript. Research in the author's laboratory is supported by the Deutsche Forschungsgemeinschaft and the Volkswagen Stiftung.
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Supplementary information
41580_2004_BFnrm1404_MOESM1_ESM.gif
supplementary information S1S1 (movie) | The Gierer-Meinhardt model. The movie illustrates how a de novo pattern is generated according to this theoretical model. The top panel shows the spatial distribution of the activity of the activator and the bottom panel shows that of the inhibitor. Please note that maximum activities of the activator and the inhibitor colocalize. This movie is courtesy of H. Meinhardt. (GIF 399 kb)
41580_2004_BFnrm1404_MOESM2_ESM.mp4
supplementary information S2S2 (movie) | Peroxisome movement in a wild-type trichome. A wild-type trichome that expresses peroxisome-targeted green fluorescent protein (GFP) is shown. Peroxisome movement depends on the actin cytoskeleton and in the wild-type trichome, peroxisomes move rapidly over long distances. This movie is courtesy of J. Mathur. (MP4 123 kb)
41580_2004_BFnrm1404_MOESM3_ESM.mov
supplementary information S3S3 (movie) | Peroxisome movement in a crooked-mutant trichome. A crooked-mutant trichome that expresses peroxisometargeted green fluorescent protein (GFP) is shown. Peroxisome movement depends on the actin cytoskeleton and the movie shows that, despite the defects in the actin organization, peroxisome movement is not generally affected. This movie is courtesy of J. Mathur. (MOV 2056 kb)
Glossary
- ENDOREDUPLICATION
-
A modified cell cycle in which DNA replication continues in the absence of mitosis and cytokinesis.
- PROTODERMAL CELL
-
A young epidermal cell that has not yet differentiated into a specialized cell type.
- MYB-RELATED TRANSCRIPTION FACTOR
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A transcription factor that contains a DNA-binding domain that shows sequence similarity to vMYB, the first-described member of this family.
- BASIC HELIX–LOOP–HELIX (BHLH) FACTOR
-
A protein that contains two α-helices separated by a loop (the HLH domain), which binds DNA in a sequence-specific manner.
- WD40 PROTEIN
-
A 40-amino-acid-long protein motif that contains a WD dipeptide at its carboxy terminus. This domain is found in many functionally diverse proteins and mediates protein–protein interactions.
- CORTEX CELL
-
The tissue between the vascular bundle and the epidermis. In A. thaliana, this is a single cell layer.
- PLASMODESMATA
-
Cell–cell connections in plants through which macromolecules, including RNA and proteins, can be transported in a regulated manner.
- B-TYPE AND D-TYPE CYCLINS
-
Cyclins regulate cell-cycle progression through interactions with cyclin-dependent protein kinases. B-type cyclins regulate entry into mitosis, whereas D-type cyclins are important in G1 phase and, in plants, also for entry into mitosis.
- DNA TOPOISOMERASE
-
An enzyme that can cleave and religate the DNA to allow a more relaxed DNA configuration.
- DECATENATE
-
During DNA replication, sister duplex molecules become interlinked (catenated). Decatenation is the separation of two entangled chromosomes.
- UBIQUITIN E3 LIGASE
-
An enzyme that attaches ubiquitin to a protein, thereby marking it for degradation in the proteasome.
- PRE-PROPHASE BAND
-
A dense band of microtubules at the cell cortex that appears before the start of cell division in plants. Its position marks the future division plane.
- PHRAGMOPLAST
-
A fibrous structure between the daughter nuclei at telophase in plant cells; also known as the cell plate.
- ARP2/3 COMPLEX
-
(Actin-related protein 2/3). A multi-protein complex that consists of seven different proteins and initiates new actin filaments on pre-existing ones.
- CHROMOCENTRE
-
A region in plant chromosomes that comprises heterochromatin and coincides with centromeres during meiosis.
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Hülskamp, M. Plant trichomes: a model for cell differentiation. Nat Rev Mol Cell Biol 5, 471–480 (2004). https://doi.org/10.1038/nrm1404
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DOI: https://doi.org/10.1038/nrm1404
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