The authors performed an analysis of ribosome pausing in Escherichia coli and Bacillus subtilis by ribosome profiling — a technique that allows the identification of ribosome-protected mRNA by high-throughput sequencing. They show that ribosome pausing is mediated by Shine–Dalgarno (SD)-like sequences within coding regions; this pausing seems to be due to an altered 16S anti-SD sequence within the ribosome that hybridizes with the SD-like sequence. In coding sequences, SD-like sequences are disfavoured and their inclusion is a way in which translation is controlled.
ORIGINAL RESEARCH PAPER
Li, G -W. et al. The anti-Shine–Dalgarno sequence drives translational pausing and codon choice in bacteria. Nature 28 March 2012 (doi:10.1038/nature10965)
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Stower, H. Anti-Shine–Dalgarno regulation of translation. Nat Rev Genet 13, 298 (2012). https://doi.org/10.1038/nrg3233
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DOI: https://doi.org/10.1038/nrg3233
