Abstract
Protein S-acylation is a reversible post-translational lipid modification that involves linkage of a fatty acid chain predominantly to a cysteine amino acid via a thioester bond. The fatty acid molecule is primarily palmitate, thus the term ‘palmitoylation’ is more commonly used. Palmitoylation has been found to modulate all stages of protein function including maturational processing, trafficking, membrane anchoring, signaling range and efficacy, and degradation. In breast cancer, palmitoylation has been shown to control the function of commonly dysregulated genes including estrogen receptors, the epidermal growth factor (EGF) family of receptors, and cancer stem cell markers. Importantly, palmitoylation is a critical factor controlling the formation of complexes at the plasma membrane involving tetraspanins, integrins, and gene products that are key to cell–cell communication. During metastasis, cancer cells enhance their metastatic capacity by interacting with stroma and immune cells. Although aberrant palmitoylation could contribute to tumor initiation and growth, its potential role in these cell–cell interactions is of particular interest, as it may provide mechanistic insight into metastasis, including cancer cell-driven immune modulation. Compelling evidence for a role for aberrant palmitoylation in breast cancer remains to be established. To this end, in this review we summarize emerging evidence and highlight pertinent knowledge gaps, suggesting directions for future research.
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Introduction
Breast cancer is a heterogeneous disease commonly categorized into three distinct therapeutic groups based on the expression of estrogen receptor alpha (ER/ESR1), progesterone receptor (PR) and the epidermal growth factor receptor HER2/ERBB2.1 Metastasis to other organs, not cancer of the breast itself, is the major cause of mortality in patients with malignancies.2 Metastatic disease is generally understood as a linear succession of events commonly referred to as the metastatic cascade, in which cells from a primary tumor invade the local extracellular matrix and stroma, intravasate into the blood and/or lymphatic vessels, and subsequently arrest and engraft at distal sites.3 Cancer stem cells (CSCs) with enhanced capacity to disseminate from the primary tumor, and stemness and self-renewal properties that enable tumor-initiating ability at distal sites, are considered to be important drivers of metastasis.4 The activity of CSCs is also coupled with compromise or dysfunction of the immune system.4,5
Metastatic potential differs across therapeutic groups, and the location and receptor status of tumor(s) at the first site of metastasis have important consequences on the spatiotemporal progression of the disease.6 How dysregulation of the classical receptors (ESR1 and ERBB2) relates to mechanisms driving metastasis including CSC activity and immune modulation remains to be fully understood. Aberrant palmitoylation is potentially a unifying factor. Importantly, the molecular components and dynamics of the palmitome might harbor novel therapeutic targets. For instance, treatments directed at the post-translational level could mitigate dysregulation of the primary biomarkers, providing an alternative for patients who are refractory to drugs that target overexpressed receptors, or present with receptor-negative tumors. Compelling evidence for a role for aberrant palmitoylation in breast cancer remains to be established. To this end, we summarize evidence that links palmitoylation to critical hallmarks of breast cancer, and highlight knowledge gaps, suggesting directions for future research. We begin with a brief introduction to palmitoylation.
S-acylation/palmitoylation
Acylation is a protein modification in which an acyl group is covalently linked to a cysteine, serine, or threonine residue.7 Protein S-acylation is a subtype that involves linkage of the fatty acid chain predominantly to a cysteine amino acid in a thioester bond. This process was first described in viral glycoprotein maturation8 and subsequently found to be ubiquitous and highly conserved from yeast to human.7 Because the fatty acid molecule is primarily palmitate the term ‘palmitoylation’ is more commonly used, but other saturated (e.g., myristic and stearic) and unsaturated (e.g., oleic and arachidonic) fatty acids also form modifications. Acylation can also occur via an ester (O-acylation) or amide (N-acylation) linkage.9
Palmitoylation is enzymatically reversible, with variable cycling rates observed across substrates within and across proteins.10 These dynamic cycles have been shown to mediate all stages of protein function including maturational processing, trafficking, membrane anchoring, signaling range and efficacy, and degradation.9 Consequently, palmitoylation events are critical for normal cellular function and impact on diverse biological processes ranging from synaptic plasticity11 to immune regulation.12 There is evidence for non-enzymatic palmitoylation, but enzymatic mechanisms predominate. Palmitoylation and depalmitoylation processes are catalysed by protein acyltransferases (PATs) and acyl-protein thioesterases (APTs), respectively.13,14
Protein acyltransferases
PATs contain a DHHC cysteine-rich domain and were first described in yeast.15 Subsequently, 24 PATs were identified in human and designated ZDHHC1 to 24 (without ZDHHC10 and including ZDHHC11B).16 Palmitoylation proceeds via a two-step catalytic mechanism. In the first step, the cysteine residue in the DHHC motif is acylated with palmitate from palmitoyl-CoA (this step is often referred to as auto-palmitoylation, as APTs incubated with palmitoyl-CoA are spontaneously palmitoylated). In the second step, the palmitoyl moiety is transferred to the thiol of a cysteine in a protein substrate (Figure 1). In the absence of a protein substrate, hydrolysis of the ester bond results in the release of palmitate and the APT is free to repeat the process. Substrate specificity has been shown to vary across PAT family members and is yet to be fully characterized.17
Many PATs have been reported to act as tumor suppressors, oncogenes, or both.18 Indeed, following observations from murine cell studies ZDHHC2 was originally named ream, for reduced expression associated with metastasis.19 These authors noted that the gene maps to a region that is frequently lost in many cancers including breast cancer.19 PATs specifically implicated in breast cancer include ZDHHC7 and ZDHHC21, which are responsible for the palmitoylation of the sex steroid receptors estrogen (ER), progesterone (PR), and androgen (AR);20 ZDHHC17 and ZDHHC20, upregulated in breast cancer cells;21,22 and ZDHHC9, which induces hyperproliferation when overexpressed in the premalignant breast cancer cell line MCF10AT.23
Acyl-protein thioesterases
Palmitoyl protein thioesterase (PPT1), a liposomal resident, was the first enzyme shown to catalyse the removal of fatty acids from proteins. Two cytoplasmic APT enzymes were subsequently identified that also act as lysophospholipases and are thus named LYPLA1/APT1 and LYPLA2/APT2.24 Some evidence suggests that PPT1 may also be active in the cytoplasm.25 Hsu et al.26 reported that the retinoid-inducible tumor suppressor RARRES3/HRASLS4 acts as an APT to regulate Wnt proteins in breast cancer cells.26 RARRES3 was previously reported to display phospholipase PLA1/2 activities27 and concomitant lysophosphatidylcholine (LPC) O-acylation and phosphatidylethanolamine N-acylation.28 The capacity for APTs to confer multiple lipid modifications indicates that the enzymatic profile of many lipid-modifying enzymes may remain to be fully characterized. Furthermore, the interdependence and functional implications of simultaneous lipid-based events are yet to be fully explored.
As with PATs, the involvement of APTs is increasingly reported in the cancer literature. PPT1 has been associated with tumor progression and metastasis in colorectal cancer,29 is a potential biomarker for bladder cancer30 and is a direct target of the antineoplastic cyclic depsipeptide didemnin B.31 LYPLA1 is reported in depalmitoylation pathways in association with prostate cancer,32 endometrial cancer,33 and chronic lymphocytic leukemia.34 Interestingly, the Wnt family member Wnt5a promotes depalmitoylation of the cell adhesion molecules CD44 (a well-known CSC marker) and MCAM in melanoma cells via LYPLA1.35 This study found that three mutations in the ZDHHC20 PAT, observed in melanoma patients, increase the levels of palmitoylated enzyme as compared with wild type, and may therefore block capacity of the enzyme to enact the second step of palmitoylation catalysis (the transfer of palmitate from ZDHHC20 to the MCAM protein); and that depalmitoylation of MCAM alone is not necessarily sufficient to promote cell invasion, whereas overexpression of LYPLA1 is sufficient.
Palmitoylation and its possible role in breast cancer metastasis
Many genes associated with metastasis are functionally controlled by palmitoylation; however, a specific role for aberrant palmitoylation in their dysregulation or pathological activity remains to be investigated. The most-direct evidence linking perturbation of palmitoylation cycling to metastatic disease involves two proteins that modulate the post-translational modification, ZDHHC2 and RARRES3. The latter displays capacity for depalmitoylation; it was identified in gene signatures specific to bone and lung metastasis, and in breast cancer cells has been shown to modulate Wnt protein palmitoylation.26,36,37 ZDHHC2 is located at chromosome 8p21.3–22, which is frequently deleted or affected by loss of heterozygosity in metastatic cancers.18 An association between chromosome arm 8p and progression of breast cancer has been known for two decades,38,39 but the relevance of ZDHHC2 loss is only beginning to be understood.
ZDHHC2 regulates tetraspanins CD9 and CD151.40 Tetraspanins are small membrane proteins that contribute to cell motility, adhesion, invasion, and immune-signaling mechanisms. They have been implicated in many cancers, and act as both metastasis suppressors and metastasis promoters.40 How they influence metastasis appears to be cancer type-specific and depends on the composition of tetraspanin complexes. Palmitoylation is an essential mechanism controlling the formation of the complexes, which involves interactions between the tetraspanins themselves and other transmembrane and cytosolic proteins, including proteins that are also functionally regulated by palmitoylation, and have independently been implicated in metastasis-promoting mechanisms. The latter include CD44v6,41,42 EGFR,43,44 and integrin alpha 6 beta 4/ITGA6.45,46 Thus, loss of ZDHHC2 or RARRES3 indirectly impacts on mechanisms associated with metastasis by altering the interaction dynamics of their target proteins, many of which are key modulators of cell function and operate at cellular membranes. Although aberrant palmitoylation can contribute to tumor initiation and growth, its potential role in these cell–cell interactions is of particular interest as it may provide new mechanistic insights into metastatic disease.
It will be important to determine the specific implications of palmitoylation perturbation on key breast cancer metastasis genes whose functions depend on palmitoylation in other cell types. For example, in 2007 Weinberg and colleagues showed how the tumor environment facilitates metastatic spread by eliciting reversible changes in the phenotype of cancer cells via CCL5-CCR5 chemokine signaling involving mesenchymal stem cells.47 CCR5 also contributes to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in the bone cavity.48 As with most chemokines, CCR5 undergoes palmitoylation at three cysteine residues within its C-terminal region. In immune cells, palmitoylation controls transport of the receptor to the cell surface and its interaction with signaling pathways. Eliminating palmitoylation reduces surface expression by trapping the receptor in organelles, where it is degraded.49,50 Better understanding of the role for palmitoylation in controlling surface-level expression of CCR5 in breast cancer cells could elucidate its role in mediating cancer cell interactions with other cell types that support the metastasis process.
Differential perturbation of palmitoylation might also provide mechanistic insight into breast cancer subtype-specific patterns of metastasis. Here again, palmitoylation-focussed investigations are required to build on current evidence arriving from disparate studies. For example MUC1, a mucin-like protein on the apical membrane of epithelia, is associated with metastasis.51 In hamster ovary cells palmitoylation plays a role in modulating MUC1 recycling from endosomes to the plasma membrane,52 and intracellular localization of MUC1 is altered in a breast cancer subtype-specific manner during tumorigenesis.53
Palmitoylation and estrogen receptors
Estrogen receptor alpha (ESR1) is the major hormone receptor used to categorize patients with breast cancer, and most tumors are ESR1-positive.54 Expression of ESR1 in breast cancer tissue has been measured in patients for over 40 years and was initially determined using ligand-binding assays. Over time it has become increasingly clear that the function of ESR1 is highly nuanced and depends on the activity of many other molecules involved in estrogen signaling cascades,55 including other forms of estrogen receptors.56 The regulation of ESR1 continues to be elucidated57 in which the role of post-translational modifications is only beginning to be understood.58
A role for palmitoylation in estrogen-receptor signaling was first identified in 2003 when plasma membrane recruitment of an alternatively spliced isoform of ESR1, designated ER46 (46 kDa), was found to be palmitoylation dependent.59 Around the same time the Marino laboratory showed that palmitoylation targets the canonical receptors ESR1 and ESR beta (ESR2) to plasma membrane caveolae thereby enabling their subsequent interaction with signal-transduction molecules (Figure 2a).56,60 This work provided several useful insights. The sex steroid 17 beta-estradiol (E2) reduces palmitoylation in a time- and dose-dependent manner.56 Palmitoylation cycling is more rapid for ESR1 than for ESR2, and depalmitoylation of these receptors mediates opposite functions on E2-exposed cancer cells: E2-induced depalmitoylation drives proliferation pathways (ERK/MAPK, PI3K/AKT, and PKC signaling associated with cell survival and cell cycle modulation, e.g., cyclin D1), whereas depalmitoylation of ESR2 drives proapoptotic activity (p38/MAPK signaling cascade involving caspase-3 activation and PARP cleavage).61
The Levin laboratory20 extended this work by identifying a palmitoylation motif within the ligand-binding (E) domain of all sex-steroid receptors. In addition, the binding of heat shock protein (hsp)27 to the palmitoylation motif of ESR1, PR, and androgen receptor (AR) was found to promote palmitoylation. In the case of ESR1, the authors noted that palmitoylation can take place in the cytosol and involves monomeric forms of the receptor, indicating that E2 might control receptor palmitoylation by inducing dimerization and thus limiting the amount of monomeric ESR1 that is available to undergo palmitoylation.
Interestingly, palmitoylation-dependent plasma membrane localization and activation of a second isoform of ESR1, designated ER36 (36 kDa), has been described in triple-negative breast cancer cells that are generally considered to be negative for all receptors (ESR1, ERBB2, and PR).62 E2-induced ER36 was found to mediate anti-apoptotic effects in cells treated with the chemotherapeutic taxol, through two signaling pathways (Figure 2b). Importantly, both estrogens and anti-estrogens, including the common therapeutic tamoxifen, stimulate cell growth via ER36 and the MAPK/ERK pathway, with anti-estrogens showing a stronger activation of MAP/ERK.63 These observations have implications for the treatment of patients resistant to tamoxifen.
Knowledge gaps relating to estrogen receptor palmitoylation
These independent reports provide insight into the importance of palmitoylation-dependent regulation of estrogen receptor function, but many fundamental questions remain to be addressed. In addition to palmitoylation, ESR1 undergoes phosphorylation, acetylation, methylation, ubiquitination, and sumoylation.64–69 To what extent these modifications are interdependent is not clear. ESR2 has multiple isoforms for which a role for palmitoylation (and other post-translational modifications (PTMs)) is yet to be investigated. A better understanding of the post-translational landscape could explain why ESR1 and its alternative isoforms ER36 and ER46 share ligand- and DNA-binding domains but display different functions.58 Similarly, the mechanisms underlying the differential binding affinity of the receptor types for E2 and other estrogen-receptor agonists and antagonists remain elusive.70
A further gray area is the role of palmitoylation in mediating crosstalk among estrogen receptors, and also among these receptors and members of the EGF family of proteins that are also dysregulated during breast cancer. In ESR1-positive breast cancer cells, ESR1 and ER46 co-localize with EGFR and ERBB2 in lipid rafts,71 and in ESR1-negative cells a positive-feedback loop involving ER36 and EGFR promotes malignant growth.72 Differential perturbations of coactivity among the estrogen and EGF family receptors might contribute to the many different breast cancer subtypes described using whole-genome transcriptomic approaches, which often appear to be disconnected from the activity of the traditional clinical markers.73
Estrogen signaling has a complex and important role in innate and adaptive immune systems, the nuances of which are beyond the scope of this review.74–76 Of particular interest here is a study by Pelekanou et al.77 in monocytes and macrophages that identified a role for ER36 and GPER1. ER36 was shown to mediate E2-induced anti-inflammatory activity, and was transiently active within the nucleus where it physically interacted with GPER1.77 A role for palmitoylation was not investigated, but localization of ER36 to the nuclear membrane is palmitoylation dependent,62 and GPER1 is a G-protein-coupled receptor, most of which undergo palmitoylation.78 Given that nucleocytoplasmic trafficking of ER36 is dependent on exportins77 and the exportin (XPO1/CRM1) is palmitoylation-regulated and undergoes retinoylation as discussed later in this review,79–81 further post-translational mechanisms may be in play (Figure 3). However, this overall conjecture is likely oversimplified. Both membrane localization and function of ESR1, and ER36 inhibition of estrogen-dependent and estrogen-independent transactivation of ESR1 and ESR2, are palmitoylation dependent.56,63 Indeed in murine lymphocytes, fine-tuning of immune response involves a complex dynamic involving multiple forms of estrogen receptors.82 Pelekanou et al.77 evaluated all known E2-binding isoforms of ESR1, ESR2, GPER1 and AR in monocytes and macrophages, and found only GPER1 and ER36 to be expressed. However, a role for ESR1 in mediating E2-induced expression of inflammatory molecules in macrophages has been described.83 This contridiction may arise from differences in experimental conditions, or might reflect cellular environment-dependent variability in estrogen signaling. Clearly, further investigations are required to clarify the role of palmitoylation, and its perturbation, in estrogen receptor-mediated signaling in cells of the immune system; a better understanding of these mechanisms might elucidate pathways underlying cancer cell-mediated immune modulation during metastasis.
Palmitoylation and EGF family members
The epidermal growth factor receptor family is composed of four protein-tyrosine kinases (EGFR/ERBB1 and ERBB2–4) that belong to the ErbB lineage of proteins.84 Overexpression of EGFR is associated with multiple cancers, tumor progression, and metastasis.43 Investigation into the cancer-promoting protein CDCP1 in ovarian cancer has revealed a role for palmitoylation in EGF-stimulated expression of CDCP1.85 Under basal conditions CDCP1 is constitutively internalized and degraded. Following EGF exposure, palmitoylation and degradation are blocked and the lifespan of the protein is increased. This study demonstrates a link between palmitoylation events and EGFR signaling, but further work is required to elucidate the exact mechanisms involved.
EGFR function and FASN-dependent palmitoylation
Fatty acid synthase (FASN) is a major lipogenic enzyme that synthesizes palmitic acid. Overexpression of FASN in normal cells induces an oncogenic phenotype; the transformation involves enhanced lipid synthesis and an increase in phosphorylation and expression of EGFR.86 Using prostate and lung cancer cells Bollu et al.43 showed that EGFR activation involves FASN-dependent palmitoylation; this occurs intracellularly and is required for both EGFR ligand-dependent and ligand-independent activation. Treatment of cells with the FASN inhibitor cerulenin or the palmitoylation inhibitor 2-bromopalmitate (2-BP) reduces EGFR levels at the plasma membrane, and concurrently increases levels at lysosomes. The relationship between FASN and EGFR appears to be bidirectional, and Bollu et al. also demonstrated a link between plasma membrane EFGR (pmEGFR) signaling and EGFR present in mitochondria (mtEGFR). Here EGF-activated pmEGFR enhances FASN activity and thus the de novo production of palmitate, which subsequently acts as a signal molecule for mtEGFR, inducing palmitoylation-dependent activation of the receptor.87 Bollu and colleagues emphasize the implications of their findings in regard to therapeutic approaches that have been developed based on the understanding of ligand-dependent and EGFR kinase activity-dependant mechanisms; awareness of signaling circuits that are independent of ligand and kinase activity provides opportunity to improve drug efficacy and to better understand pathways to resistance.
Evidence for palmitoylation-dependent regulation of ERBB2
ERBB2 (also referred to as HER2/neu) is expressed at low levels in normal breast epithelial cells88 and overexpressed in about 15 to 25% of breast cancers.89 ERBB2 was first identified as a tumor antigen in cell lines from rat in which neuro/glioblastoma cancer had been chemically induced.90 ERBB2 is unique within the EGF family in that no known soluble ligand has been identified, and it does not contain a key intramolecular ‘tether’ in the extracellular region that autoinhibits other human EGF family members.91 As with EGFR, FASN phosphorylation, and subsequent activity have been associated with ERBB2 overexpression,92,93 and in one study changes in protein level were not reflected at the messenger RNA levels indicating that FASN-ERBB2 interaction is mediated post-transcriptionally. The Bollu group found that the cysteine residue for EGFR palmitoylation (Cys797) is conserved across EGFR, ERBB2 and ERBB4, all of which exhibit kinase activity, but is substituted by a serine in ERBB3, which lacks kinase activity. Furthermore, mutation of Cys797 in each EGFR family member blocked palmitoylation and ERK signaling in non-cancer cell lines (HEK293T).43
Prior to the work by Bollu et al., a crystallographic study hinted at the possibility of palmitoylation-dependent interaction between ERBB2 and non-soluble proteins.91 The authors conducted structural comparisons between human ERBB2 and the single EGF receptor in Drosophila melanogaster dEGFR. The latter has four ligands, one of which (Spitz) has been shown to require palmitoylation for plasma membrane association, and two others (Gurken and Keren) contain a similar palmitoylation site. The structural similarity of ERBB2 to dEGFR prompted the authors to posit that ERBB2 and dEGFR may both be regulated via palmitoylation-dependent activation involving membrane-bound as opposed to soluble ligands. In human, proteins that support this conjecture include Mucin 4 (MUC4) and the ERBB2-interacting protein ERBB2IP. The latter, also known as Erbin, was first described as a binding partner of ERBB2, and is dependent on palmitoylation for membrane localization. ERBB2IP is implicated in ERBB2-dependent tumor growth and cell migration.94–96 MUC4 is a cell-surface glycoprotein that also interacts with ERBB2 and promotes its tyrosine phosphorylation.97 At the time of writing, no reports describing palmitoylation of MUC4 have been identified but the modification is reported for other members of the mucin family: Mucin 2 (MUC2) undergoes N-terminal palmitoylation and is regulated by FASN in the colon;98 and recycling to the cell surface of Mucin 1 (MUC1), which modulates EGFR activity, requires palmitoylation of both cysteines within a CQC motif at the boundary of the transmembrane and cytoplasmic domains.52 Interestingly, both ERBB2IP and MUC4 have been associated with resistance to the ERBB2 antibody trastuzumab.94,99
Important knowledge gaps relating to EGF signaling
Palmitoylation of integrin subunits beta-4 (ITGB4), alpha-3 (ITGA3), and alpha-6 (ITGA6) impacts on the formation of integrin tetraspanin complexes.100 ITGA6 and ITGB4 cooperate with EGFR and ERBB2 to facilitate multiple aspects of tumor progression and metastasis.101 For example, in a mouse model of ERBB2-mediated breast cancer, ITGB4 forms a complex with ERBB2 and amplifies ERBB2 signaling. Loss of ITGB4 in this model reduced invasive growth and metastasis.102 Given recent evidence showing a role for palmitoylation in EGF receptor signaling it will be important to undertake studies to determine how different palmitoylation perturbation pathways impact on EGF receptor-integrin dynamics within tetraspanin complexes. It is envisaged that mutation-based approaches that eliminate the activity of specific palmitoyltransferases and/or target substrates will be useful in this regard.
Palmitoylation and breast cancer stem cell markers
Inflammatory conditions within the microenvironment and aberrant epithelial–mesenchymal transition (EMT) are thought to confer stem cell properties to carcinoma cells.4 These transformed cells have enhanced capacity for migration and invasion and are commonly referred to as cancer stem cells (CSCs). CSCs are characterized by the expression of specific surface markers including EpCAM+ CD24−/low/CD44+/high and ALDH+. The latter cells are epithelial-like and associate with a self-renewal phenotype, whereas EpCAM+ CD24−/CD44+ are associated with the metastatic and aggressive mesenchymal phenotype.103 CD44 is a large transmembrane glycoprotein that acts as a receptor for hyaluronic acid. Transcripts of this gene undergo complex alternative splicing to produce functionally distinct isoforms, and several post-translational modifications including palmitoylation have been reported.104 Specifically, the mutation of either of two highly conserved cysteine residues reduces the incorporation of palmitate, prevents association with CD44-lipid rafts, and inhibits the internalization of hyaluronan.105 Palmitoylation is thus a crucial determinant of CD44 turnover at the cell surface, and of hyaluronic acid endocytosis. In a rat model of colorectal cancer, a specific isoform of CD44, CD44v6, forms a complex with Claudin-7, a tetraspanin (TSPAN8/ CO-029) and EpCam. It has been suggested that this complex, rather than the individual proteins, supports tumor progression and metastasis.106
Gaps in current knowledge
EpCam co-localizes with CD44, Claudin-7, and TSPAN8 in tetraspanin-enriched membrane microdomains. The role of palmitoylation in the formation of these membrane complexes is only partially understood. The stabilization and interaction among tetraspanins is reported to be palmitoylation-dependent, but a role specific to TSPAN8 function is yet to be investigated.107 Palmitoylation of Claudin-7 is essential for association with, and recruitment of, EpCAM to glycolipid-enriched membrane domains in HEK cells.108 At the time of writing, no reports describing palmitoylation of EpCAM itself have been identified, but an extracellular domain within the N-terminal of the protein contains tandem EGF-like repeats.109 Mutational approaches show that these repeat domains are essential for accumulation of EpCAM molecules at cell–cell boundaries, lateral interaction between molecules, and anchorage to F-actin, all of which could potentially be palmitoylation dependent.109
Palmitoylation and immune modulation
To maintain immune homeostasis, pro-, and anti-inflammatory pathways act concurrently as opposing forces. Perturbation of the dynamic balance between them leads to immune-mediated disease including cancer progression and metastasis.5 In the latter, perturbation of cell population dynamics is an emerging theme. Essentially, the ratios between different phenotypes of the same cell-type become skewed, causing a pathological tip in the equilibrium toward either pro- or anti-inflammatory conditions. A study using a murine model of spontaneous breast cancer provides a case in point.110 This study describes a domino effect in which tumor-induced inflammation triggers signaling that leads to loss of the cytotoxic CD8 T lymphocytes that would normally supress neoplasia and metastasis. Cell-depletion approaches demonstrated that neutrophils were essential to this domino effect. The proportions of CD8+ T cells in the lungs of control and neutrophil-depleted tumor-bearing mice were similar, but importantly the number of (metastasis-preventing) effector T cells was enhanced in the latter. Although this example is specific to one mouse model, the concepts described, in particular changes in ratios between phenotypes of the same cell population, are increasingly reported in the breast cancer metastasis literature. In addition to the many subtypes of T cells, neutrophils, and macrophages exhibit both pro- (N1, M1) and anti-inflammatory (N2, M2) phenotypes, the ratio of which can become skewed within the cancer microenvironment. This domino affect involves upregulation of NOS2 by >150-fold.110 NOS2 encodes inducible nitric oxide synthase (iNOS), and palmitoylation of this protein is required for its proper sorting, localization to the endoplasmic reticulum, and nitric oxide synthetic activity.111 It will be important to determine whether, and how, aberrant palmitoylation contributes to the skewing of cell phenotypes as described during the domino effect.
The palmitome of primary and Jurkat T cells was recently evaluated, revealing a pool of 120 palmitoylated proteins common to both cell types. This pool contained well-known palmitoylated proteins plus 92 previously unreported as palmitoylated in vivo.81 Known palmitoylated proteins include the breast cancer-related proteins ERBB2IP, FASN, CD44, and products of 52 genes implicated in breast cancer metastasis to bone, lung, or brain (Table 1). The T-cell study also showed that some PAT enzymes are themselves palmitoylated, indicating a feedback mechanism, and differential palmitoylation of T-cell surface antigen (mono- versus dual-lipidation) was observed that likely affords greater functional plasticity. The T-cell receptor (TCR) is purported to be one of the best examples of the importance of protein palmitoylation for cell signaling.112 Although the TCR subunits do not undergo palmitoylation, the functions of co-receptors CD4 and CD8 and associated signaling molecules including SRC family kinases LCK and FYN and linker for activation of T cells are palmitoylation dependent. Altogether, these lines of evidence provide insight into how global changes in palmitoylation dynamics might be a common feature linking dysregulation of classical breast cancer biomarkers, stem cell surface markers and immune cell function.
Knowledge gaps in relation to chemokine signaling
Chemokines have a critical role in immune cell function including, for example, maintaining the balance between neutrophil release and retention.113 By analogy with palmitoylation-dependent function in immune cells, potential involvement of aberrant palmitoylation in breast cancer warrants clarification. In addition to the palmitoylation-dependent function of the CCR5 receptor (above), in T lymphocytes the function of another breast cancer-associated ligand, CXCL12, depends on dual acylation with myristic and palmitic moieties of a specific isoform of the SRC-family kinase member HCK.114 In another example, the ligand CCL18 has been identified as the main driving factor in a deleterious positive-feedback loop, in which CSCs activate macrophages with an anti-inflammatory M2-like phenotype. Reciprocally, the latter express EMT-inducing molecules that sustain the CSC population.115
Chemokine receptors are G-protein-coupled receptors (GPCR), most of which undergo palmitoylation at one, two, or three residues in the carboxyl-terminal cytoplasmic tail. All aspects of GPCR function including coupling to G proteins and strength of signaling have been associated with palmitoylation; however, effects vary across receptor types, and in many cases are yet to be fully characterized.78 Similarly, the regulation of palmitoylation depends on the type of receptor and involves a variety of mechanisms including activation by an agonist, changes in nitric oxide levels and/or lipid raft conditions (e.g., cholesterol depletion). Important questions remain to be addressed including why the attachment of palmitate to the same domain can subserve the many different functional roles of GPCR proteins.78 It is likely that different combinations of PTMs acting concurrently within a protein, and/or PTM events occurring simultaneously across multiple proteins, coenact specific biological effects. Palmitoylation-dependent regulation of chemokines is less understood, but likely mirrors the complexity and variation described for the broader family of GPCRs.
Interplay among PTMs including retinoylation
Palmitoylation events are part of a complex interplay involving several types of PTMs. Multiple lipid modifications can co-occur (e.g., palmitoylation and prenylation and/or myristoylation)116 and can act concurrently, sequentially, or antagonistically with other protein modifications including phosphorylation and ubiquitination.117,118 It has been shown, for instance, that O-linked acylation of serine is required for subsequent palmitoylation of cysteine in mouse Wnt proteins,119 and that palmitoylation enhances the interferon-induced transmembrane protein IFITM3, while ubiquitination decreases activity.118 Overall, the interdependency of PTMs occurring within a single protein is only just beginning to be described. Of particular interest to breast cancer biology is retinoylation (acylation by retinoic acid).
Takahashi and Breitman120 first presented evidence for covalent attachment of retinoic acid (RA) to protein in 1989. They subsequently showed that some proteins from MCF-7 cells undergo retinoylation and are also targets for E2 ligands.121 Treatment with tunicamycin, an inhibitor of both protein N-glycosylation and palmitoylation, decreased palmitoylation while enhancing retinoylation of proteins, indicating a potential opposing relationship between these modifications.122 XPO1 is an example of a protein potentially regulated by both palmitoylation and retinoylation, with functional relevance to breast cancer.
The karyopherins (e.g., KPNA1 and KNPB1) and XPO1 are nuclear transporters that shuttle proteins between the nucleus and the cytoplasm.123 Analysis of cultured rat embryonic neurons identified XPO1 as a potential candidate for palmitoylation.124 This protein is also a member of the palmitome of human primary and Jurkat T cells.81 Curcumin, which has been shown to block the auto-acylation step of the ZDHHC3 PAT,125 was found to target XPO1 and specifically interact with cysteine (Cys528).126 XPO1 undergoes retinoylation at the same residue that is targeted by curcumin (Cys528).79 Altogether, these lines of evidence suggest that XPO1 undergoes both palmitoylation and retinoylation.
Most transcription factors implicated in EMT contain conserved signal sequences that are recognized by karyopherins.123 Inhibition of XPO1 was found to reverse EMT in snail-transduced primary human mammary epithelial cells, and to completely eliminate tumors in a xenograft mouse model.123 XPO1-dependent protein shuttling is also important within the immune context. For example, the cellular release of proinflammatory TNF-alpha from stimulated neutrophils is XPO1-dependent.127 It would be interesting to determine whether retinoylation and palmitoylation differentially impact on XPO1 function, and if so whether perturbation of this dynamic contributes to breast cancer metastasis.
Retinoylation is inhibited or enhanced by palmitic and arachidonic acids, respectively. These fatty acids are products of phospholipase 2 (PLA2), and PLA2 activity is part of the functional repertoire of the tumor suppressor RARRES3. This gene was initially named retinoid acid receptor responder (tazarotene induced) 3 (RARRES3) due to its upregulation in human keratinocytes that had been exposed to the synthetic retinoid tazarotene.128 It has been extensively renamed and is most recently described as member 4 of the H-RAS-like suppressor (HRASLS) subfamily (HRASLS4). This family consists of five enzymes that display phospholipase A1/2 and N- and O-acyltransferase activities.27 The tumor suppressor effects of RARRES3 have been attributed, by two investigative studies, to its activity as a phospholipase129 and, as mentioned earlier in this review, as an APT (due to its capacity to reduce palmitoylation levels of Wnt proteins).26 In the former study, phospholipase activity was associated with cellular changes in arachidonic acid. It is interesting to speculate that increased arachidonic acid, and possibly subsequent increase in cellular retinoylation, may have indirectly contributed to the reduced Wnt protein palmitoylation observed in the latter study, as opposed to direct ATP-enacted depalmitoylation.
Conclusions
Aberrant protein palmitoylation during cancer represents an area of increasing interest. Research efforts have focussed on palmitoylation, but other forms of S-acylation likely have important roles in protein regulation. The lines of evidence presented herein indicate that aberrant palmitoylation may be a factor common to the dysregulation of primary breast cancer markers, chemokines and other molecules involved in tumor-induced immunosuppression. These insights raise interesting avenues for future research. For instance, viewing the relationship between estrogen and retinoid-related signaling through the lens of palmitoylation and retinoylation dynamics might improve our understanding of estrogen/retinoic acid antagonism and/or agonism described in breast cancer.130,131 Research into the relationship between estrogen and retinoid pathways has primarily focussed on classical retinoid acid receptor signaling, whereas non-classical pathways described in retinoylation,79 and in regard to the role of aldehyde dehydrogenases and cancer stem cells132 are perhaps more worthy of investigation within the context of post-translational protein regulation. Similarly, the interplay and possible interdependency among PTMs is only just beginning to be understood; perturbations arising here may represent causal mechanisms that drive ‘symptoms’ presenting at the transcriptional level, elucidating potent therapeutic targets. The development of highly specific palmitoylation inhibitors will likely prove important, but it may be that dysfunction is a consequence of substrate competition or imbalance in the cellular concentrations of palmitate and other moieties (e.g., arachidonic acid) as opposed to aberrant function of PATs and APTs.
Palmitoylation-specific investigations are required to help address the many fundamental questions that remain unanswered. It would be useful to evaluate the palmitome in CSCs extracted from patients with different breast cancer subtypes and/or stages of disease; compare palmitoylation events between tumor and control samples; and assess the influence of estrogen, retinoid, and EGF signaling pathways on known palmitoylation-dependent proteins that regulate immune cells within the tumor microenvironment.
We believe that RARRES3 is an ideal candidate for further enquiry at the enzymatic level to clarify, for instance, the functional consequences of multiple lipid-related modifications conferred concurrently or in rapid succession by a single enzyme. It is also possible that this enzyme has capacity to mediate retinoylation, for which enzymatic modifiers have yet to be described. We expect that a more-comprehensive understanding of palmitoylation will lead to novel therapeutic strategies for prevention and cure of breast cancer metastasis.
References
Koboldt, D. C. et al. Comprehensive molecular portraits of human breast tumours. Nature 490, 61–70 (2012).
Gupta, G. P. & Massague, J. Cancer metastasis: Building a framework. Cell 127, 679–695 (2006).
Valastyan, S. & Weinberg, R. A. Tumor metastasis: Molecular insights and evolving paradigms. Cell 147, 275–292 (2011).
Bottoni, P., Isgro, M. A. & Scatena, R. The epithelial-mesenchymal transition in cancer: a potential critical topic for translational proteomic research. Expert Rev. Proteomics 13, 115–133 (2016).
Crusz, S. M. & Balkwill, F. R. Inflammation and cancer: advances and new agents. Nat. Rev. Clin. Oncol. 12, 584–596 (2015).
Newton, P. K. et al. Spatiotemporal progression of metastatic breast cancer: a Markov chain model highlighting the role of early metastatic sites. Npj Breast Cancer 1, 15018 (2015).
Towler, D. A., Gordon, J. I., Adams, S. P. & Glaser, L. The biology and enzymology of eukaryotic protein acylation. Annu. Rev. Biochem. 57, 69–99 (1988).
Schmidt, M. F. G. & Schlesinger, M. J. Fatty acid binding to vesicular stomatitis virus glycoprotein: a new type of post-translational modification of the viral glycoprotein. Cell 17, 813–819 (1979).
Chamberlain, L. H. & Shipston, M. J. The physiology of protein S-acylation. Physiol. Rev. 95, 341–376 (2015).
Chamberlain, L. H. et al. Palmitoylation and the trafficking of peripheral membrane proteins. Biochem. Soc. Trans. 41, 62–66 (2013).
Brigidi, G. S., Santyr, B., Shimell, J., Jovellar, B. & Bamji, S. X. Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5. Nat. Commun. 6, 17 (2015).
Liu, Z. et al. Integrative chemical biology approaches for identification and characterization of ‘erasers’ for fatty-acid-acylated lysine residues within proteins. Angew. Chem. Int. Ed. 54, 1149–1152 (2015).
Zeidman, R., Jackson, C. S. & Magee, A. I. Protein acyl thioesterases (Review). Mol. Membr. Biol. 26, 32–41 (2009).
Vartak, N. et al. The autodepalmitoylating activity of APT maintains the spatial organization of palmitoylated membrane proteins. Biophys. J. 106, 93–105 (2014).
Lobo, S., Greentree, W. K., Linder, M. E. & Deschenes, R. J. Identification of a Ras palmitoyltransferase in Saccharomyces cerevisiae. J. Biol. Chem. 277, 41268–41273 (2002).
Lemonidis, K. et al. The zDHHC family of S-acyltransferases. Biochem. Soc. Trans. 43, 217–221 (2015).
Hornemann, T. Palmitoylation and depalmitoylation defects. J. Inherit. Metab. Dis. 38, 179–186 (2015).
Yeste-Velasco, M., Linder, M. E. & Lu, Y. J. Protein S-palmitoylation and cancer. Biochim. Biophys. Acta 1856, 107–120 (2015).
Oyama, T. et al. Isolation of a novel gene on 8p21.3-22 whose expression is reduced significantly in human colorectal cancers with liver metastasis. Genes Chromosomes Cancer 29, 9–15 (2000).
Pedram, A., Razandi, M., Deschenes, R. J. & Levin, E. R. DHHC-7 and-21 are palmitoylacyltransferases for sex steroid receptors. Mol. Biol. Cell 23, 188–199 (2012).
Ducker, C. E. et al. Discovery and characterization of inhibitors of human palmitoyl acyltransferases. Mol. Cancer Ther. 5, 1647–1659 (2006).
Draper, J. M. & Smith, C. D. DHHC20: a human palmitoyl acyltransferase that causes cellular transformation. Mol. Membr. Biol. 27, 123–136 (2010).
Young, E. et al. Regulation of Ras localization and cell transformation by evolutionarily conserved palmitoyltransferases. Mol. Cell Biol. 34, 374–385 (2014).
Lin, D. T. S. & Conibear, E. Enzymatic protein depalmitoylation by acyl protein thioesterases. Biochem. Soc. Trans. 43, 193–198 (2015).
Kim, S. J. et al. Palmitoyl protein thioesterase-1 deficiency impairs synaptic vesicle recycling at nerve terminals, contributing to neuropathology in humans and mice. J. Clin. Invest. 118, 3075–3086 (2008).
Hsu, T. H. et al. Involvement of RARRES3 in the regulation of Wnt proteins acylation and signaling activities in human breast cancer cells. Cell Death Differ. 22, 801–814 (2015).
Mardian, E. B., Bradley, R. M. & Duncan, R. E. The HRASLS (PLA/AT) subfamily of enzymes. J. Biomed. Sci. 22, 1–11 (2015).
Uyama, T., Jin, X. H., Tsuboi, K., Tonai, T. & Ueda, N. Characterization of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes. Biochim. Biophys. Acta 1791, 1114–1124 (2009).
Tsukamoto, T., Iida, J., Dobashi, Y., Furukawa, T. & Konishi, F. Overexpression in colorectal carcinoma of two lysosomal enzymes, CLN2 and CLN1, involved in neuronal ceroid lipofuscinosis. Cancer 106, 1489–1497 (2006).
Su, C. -C. et al. An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells. Mar. Drugs 9, 2622–2642 (2011).
Potts, M. B. et al. Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nat. Chem. Biol. 11, 401-+ (2015).
Davalieva, K. et al. Proteomics analysis of malignant and benign prostate tissue by 2D DIGE/MS reveals new insights into proteins involved in prostate cancer. Prostate 75, 1586–1600 (2015).
Trousil, S. et al. Alterations of choline phospholipid metabolism in endometrial cancer are caused by choline kinase alpha overexpression and a hyperactivated deacylation pathway. Cancer Res. 74, 6867–6877 (2014).
Berg, V. et al. miRs-138 and-424 control palmitoylation-dependent CD95-mediated cell death by targeting acyl protein thioesterases 1 and 2 in CLL. Blood 125, 2948–2957 (2015).
Wang, W., Runkle, K. B., Terkowski, S. M., Ekaireb, R. I. & Witze, E. S. Protein depalmitoylation is induced by Wnt5a and promotes polarized cell behavior. J. Biol. Chem. 290, 15707–15716 (2015).
Bos, P. D. et al. Genes that mediate breast cancer metastasis to the brain. Nature 459, 1005–U137 (2009).
Kang, Y. B. et al. A multigenic program mediating breast cancer metastasis to bone. Cancer Cell 3, 537–549 (2003).
Yaremko, M. L. et al. Loss of heterozygosity from the short arm of chromosome 8 is associated with invasive behavior in breast cancer. Genes Chromosomes Cancer 16, 189–195 (1996).
Anbazhagan, R., Fujii, H. & Gabrielson, E. Allelic loss of chromosomal arm 8p in breast cancer progression. Am. J. Pathol. 152, 815–819 (1998).
Zoller, M. Tetraspanins: push and pull in suppressing and promoting metastasis. Nat. Rev. Cancer 9, 40–55 (2009).
Babina, I. S., McSherry, E. A., Donatello, S., Hill, A. D. K. & Hopkins, A. M. A novel mechanism of regulating breast cancer cell migration via palmitoylation-dependent alterations in the lipid raft affiliation of CD44. Breast Cancer Res. 16, R19 (2014).
Diaz, L. K. et al. C44 expression is associated with increased survival in node-negative invasive breast carcinoma. Clinical Cancer Res. 11, 3309–3314 (2005).
Bollu, L. R. et al. Intracellular activation of EGFR by fatty acid synthase dependent palmitoylation. Oncotarget 6, 34992–35003 (2015).
Lim, S. O. et al. EGFR signaling enhances aerobic glycolysis in triple-negative breast cancer cells to promote tumor growth and immune escape. Cancer Res. 76, 1284–1296 (2016).
Sharma, C., Rabinovitz, I. & Hemler, M. E. Palmitoylation by DHHC3 is critical for the function, expression, and stability of integrin alpha 6 beta 4. Cell Mol. Life Sci. 69, 2233–2244 (2012).
Chen, M. & O'Connor, K. L. Integrin alpha 6 beta 4 promotes expression of autotaxin/ENPP2 autocrine motility factor in breast carcinoma cells. Oncogene 24, 5125–5130 (2005).
Karnoub, A. E. et al. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature 449, 557–U4 (2007).
Sasaki, S. et al. Essential roles of the interaction between cancer cell-derived chemokine, CCL4, and intra-bone CCR5-expressing fibroblasts in breast cancer bone metastasis. Cancer Lett. 378, 23–32 (2016).
Barmania, F. & Pepper, M. S. C-C chemokine receptor type five (CCR5): An emerging target for the control of HIV infection. Appl. Transl. Genomics 2, 3–16 (2013).
Percherancier, Y. et al. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J. Biol. Chem. 276, 31936–31944 (2001).
Horm, T. M. & Schroeder, J. A. MUC1 and metastatic cancer Expression, function and therapeutic targeting. Cell Adh. Migr. 7, 187–198 (2013).
Kinlough, C. L. et al. Recycling of MUC1 is dependent on its palmitoylation. J. Biol. Chem. 281, 12112–12122 (2006).
Iizuka, M. et al. Altered intracellular region of MUC1 and disrupted correlation of polarity-related molecules in breast cancer subtypes. Cancer Sci. 106, 307–314 (2015).
Allred, D. C. Issues and updates: evaluating estrogen receptor-alpha, progesterone receptor, and HER2 in breast cancer. Mod. Pathol. 23, S52–S59 (2010).
Stellato, C. et al. Identification of cytoplasmic proteins interacting with unliganded estrogen receptor and in human breast cancer cells. Proteomics 15, 1801–1807 (2015).
Acconcia, F. et al. Palmitoylation-dependent estrogen receptor alpha membrane localization: regulation by 17 beta-estradiol. Mol. Biol. Cell 16, 231–237 (2005).
Bennesch, M. A. & Picard, D. Minireview: Tipping the balance: ligand-independent activation of steroid receptors. Mol. Endocrinol. 29, 349–363 (2015).
Le Romancer, M. et al. Cracking the estrogen receptor's posttranslational code in breast tumors. Endocr. Rev. 32, 597–622 (2011).
Li, L., Haynes, M. P. & Bender, J. R. Plasma membrane localization and function of the estrogen receptor alpha variant (ER46) in human endothelial cells. Proc. Natl Acad. Sci. USA 100, 4807–4812 (2003).
Acconcia, F., Ascenzi, P., Fabozzi, G., Visca, P. & Marino, M. S-palmitoylation modulates human estrogen receptor-alpha functions. Biochem. Biophys. Res. Commun. 316, 878–883 (2004).
Galluzzo, P., Caiazza, F., Moreno, S. & Marino, M. Role of ER beta palmitoylation in the inhibition of human colon cancer cell proliferation. Endocr. Relat. Cancer 14, 153–167 (2007).
Chaudhri, R. A., Hadadi, A., Lobachev, K. S., Schwartz, Z. & Boyan, B. D. Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism. Biochim. Biophys. Acta 1843, 2796–2806 (2014).
Wang, Z. Y. et al. A variant of estrogen receptor-alpha, hER-alpha 36: Transduction of estrogen- and antiestrogen-dependent membrane-initiated mitogenic signaling. Proc. Natl Acad. Sci. USA 103, 9063–9068 (2006).
La Rosa, P., Pesiri, V., Leclercq, G., Marino, M. & Acconcia, F. Palmitoylation regulates 17 beta-estradiol-induced estrogen receptor-alpha degradation and transcriptional activity. Mol. Endocrinol. 26, 762–774 (2012).
Murphy, L. C., Seekallu, S. V. & Watson, P. H. Clinical significance of estrogen receptor phosphorylation. Endocr. Relat. Cancer. 18, R1–R14 (2011).
Wang, C. G. et al. Direct acetylation of the estrogen receptor alpha hinge region by p300 regulates transactivation and hormone sensitivity. J. Biol. Chem. 276, 18375–18383 (2001).
Subramanian, K. et al. Regulation of estrogen receptor alpha by the SET7 lysine methyltransferase. Mol. Cell 30, 336–347 (2008).
Ma, Y. X. et al. BRCA1 regulates acetylation and ubiquitination of estrogen receptor-alpha. Mol. Endocrinol. 24, 76–90 (2010).
Picard, N. et al. Identification of estrogen receptor beta as a SUMO-1 target reveals a novel phosphorylated sumoylation motif and regulation by glycogen synthase kinase 3 beta. Mol. Cell Biol. 32, 2709–2721 (2012).
Lin, A. H. Y. et al. Differential ligand binding affinities of human estrogen receptor-alpha isoforms. PLoS ONE 8, 9 (2013).
Marquez, D. C., Chen, H. W., Curran, E. M., Welshons, W. V. & Pietras, R. J. Estrogen receptors in membrane lipid rafts and signal transduction in breast cancer. Mol. Cell. Endocrinol. 246, 91–100 (2006).
Zhang, X. T. et al. A positive feedback loop of ER-alpha 36/EGFR promotes malignant growth of ER-negative breast cancer cells. Oncogene 30, 770–780 (2011).
Taherian-Fard, A., Srihari, S. & Ragan, M. A. Breast cancer classification: linking molecular mechanisms to disease prognosis. Brief. Bioinformatics 16, 461–474 (2015).
Frasor, J. et al. Positive cross-talk between estrogen receptor and NF-kB in breast cancer. Cancer Res. 69, 8918–8925 (2009).
Khan, D. & Ahmed, S. A. The immune system is a natural target for estrogen action: opposing effects of estrogen in two prototypical autoimmune diseases. Front. Immunol. 6, 8 (2016).
Kovats, S. Estrogen receptors regulate innate immune cells and signaling pathways. Cell Immunol. 294, 63–69 (2015).
Pelekanou, V. et al. Estrogen anti-inflammatory activity on human monocytes is mediated through cross-talk between estrogen receptor ERalpha36 and GPR30/GPER1. J. Leukoc. Biol. 99, 333–347 (2016).
Chini, B. & Parenti, M. G-protein-coupled receptors, cholesterol and palmitoylation: facts about fats. J. Mol. Endocrinol. 42, 371–379 (2009).
Dave, S. et al. Nuclear MEK1 sequesters PPAR gamma and bisects MEK1/ERK signaling: a non-canonical pathway of retinoic acid Inhibition of adipocyte differentiation. PLoS ONE 9 (6), e100862 (2014).
Kang, R. J. et al. Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation. Nature 456, 904–909 (2008).
Morrison, E. et al. Quantitative analysis of the human T cell palmitome. Sci. Rep. 5, 7 (2015).
Schneider, A. E. et al. A dynamic network of estrogen receptors in murine lymphocytes: fine-tuning the immune response. J. Leukoc. Biol. 96, 857–872 (2014).
Calippe, B. et al. 17 beta-Estradiol promotes TLR4-triggered proinflammatory mediator production through direct estrogen receptor alpha signaling in macrophages in vivo. J. Immunol. 185, 1169–1176 (2010).
Roskoski, R. Jr ErbB/HER protein-tyrosine kinases: Structures and small molecule inhibitors. Pharmacol. Res. 87, 42–59 (2014).
Adams, M. N. et al. EGF inhibits constitutive internalization and palmitoylation-dependent degradation of membrane-spanning procancer CDCP1 promoting its availability on the cell surface. Oncogene 34, 1375–1383 (2015).
Vazquez-Martin, A., Colomer, R., Brunet, J., Lupu, R. & Menendez, J. A. Overexpression of fatty acid synthase gene activates HER1/HER2 tyrosine kinase receptors in human breast epithelial cells. Cell Prolif. 41, 59–85 (2008).
Bollu, L. R. et al. Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells. Cell Cycle 13, 2415–2430 (2014).
Press, M. F., Cordoncardo, C. & Slamon, D. J. Expression of the HER-2/neu protooncogenein normal human adult and fetal tissues. Oncogene 5, 953–962 (1990).
Krishnamurti, U. & Silverman, J. F. HER2 in breast cancer: a review and update. Adv. Anat. Pathol. 21, 100–107 (2014).
Schechter, A. L. et al. The neu oncogene—an ERB-B-related gene encoding a 185,000-Mr tumour-antigen. Nature 312, 513–516 (1984).
Alvarado, D., Klein, D. E. & Lemmon, M. A. ErbB2/HER2/Neu resembles an autoinhibited invertebrate epidermal growth factor receptor. Nature 461, 287–291 (2009).
Jin, Q. R. et al. Fatty acid synthase phosphorylation: a novel therapeutic target in HER2-overexpressing breast cancer cells. Breast Cancer Res. 12, 15 (2010).
Yoon, S. et al. Up-regulation of Acetyl-CoA carboxylase alpha and fatty acid synthase by human epidermal growth factor receptor 2 at the translational level in breast cancer cells. J. Biol. Chem. 282, 26122–26131 (2007).
Liu, D. et al. Downregulation of Erbin in Her2-overexpressing breast cancer cells promotes cell migration and induces trastuzumab resistance. Mol. Immunol. 56, 104–112 (2013).
Tao, Y. M. et al. Role of Erbin in ErbB2-dependent breast tumor growth. Proc. Natl Acad. Sci. USA 111, E4429–E4438 (2014).
Izawa, I., Nishizawa, M., Hayashi, Y. & Inagaki, M. Palmitoylation of ERBIN is required for its plasma membrane localization. Genes Cells 13, 691–701 (2008).
Kozloski, G. A., Carraway, C. A. C. & Carraway, K. L. Mechanistic and signaling analysis of Muc4-ErbB2 signaling module: new insights into the mechanism of ligand-independent ErbB2 activity. J. Cell Physiol. 224, 649–657 (2010).
Wei, X. C. et al. Fatty acid synthase modulates intestinal barrier function through palmitoylation of Mucin 2. Cell Host Microbe 11, 140–152 (2012).
Nagy, P. et al. Decreased accessibility and lack of activation of ErbB2 in JIMT-1, a herceptin-resistant, MUC4-Expressing breast cancer cell line. Cancer Res. 65, 473–482 (2005).
Yang, X. W. et al. Palmitoylation supports assembly and function of integrin-tetraspanin complexes. J. Cell Biol. 167, 1231–1240 (2004).
Stewart, R. L. & O'Connor, K. L. Clinical significance of the integrin alpha 6 beta 4 in human malignancies. Lab Invest. 95, 976–986 (2015).
Guo, W. J. et al. beta 4 integrin amplifies ErbB2 signaling to promote mammary tumorigenesis. Cell 126, 489–502 (2006).
Luo, M., Brooks, M. & Wicha, M. S. Epithelial-mesenchymal plasticity of breast cancer stem cells: implications for metastasis and therapeutic resistance. Curr. Pharm. Des. 21, 1301–1310 (2015).
Guvench, O. Revealing the mechanisms of protein disorder and N-glycosylation in CD44-hyaluronan binding using molecular simulation. Front. Immunol. 6, 1–9 (2015).
Thankamony, S. P. & Knudson, W. Acylation of CD44 and its association with lipid rafts are required for receptor and hyaluronan endocytosis. J. Biol. Chem. 281, 34601–34609 (2006).
Kuhn, S. et al. A complex of EpCAM, claudin-7, CD44 variant isoforms, and tetraspanins promotes colorectal cancer progression. Mol. Cancer Res. 5, 553–567 (2007).
Charrin S. et al. Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation. FEBS Lett. 516, 139–144 (2002).
Heiler, S., Mu, W., Zoller, M. & Thuma, F. The importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities. Cell Commun. Signal. 13, 17 (2015).
Blazar, M. et al. Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol. Cell Biol. 21, 2570–2580 (2001).
Coffelt, S. B. et al. IL-17-producing gamma delta T cells and neutrophils conspire to promote breast cancer metastasis. Nature 522, 345-+ (2015).
Navarro-Lerida, I. et al. Palmitoylation of inducible nitric-oxide synthase at Cys-3 is required for proper intracellular traffic and nitric oxide synthesis. J. Biol. Chem. 279, 55682–55689 (2004).
Resh, M. D. Palmitoylation of ligands, receptors, and intracellular signaling molecules. Sci. STKE 2006, re14–re14 (2006).
Kruger, P. et al. Neutrophils: between host defence, immune modulation, and tissue injury. PLoS Pathog. 11 (2015).
Zaman, S. N., Resek, M. E. & Robbins, S. M. Dual acylation and lipid raft association of Src-family protein tyrosine kinases are required for SDF-1/CXCL12-mediated chemotaxis in the Jurkat human T cell lymphoma cell line. J. Leukoc. Biol. 84, 1082–1091 (2008).
Su, S. C. et al. A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell 25, 605–620 (2014).
Linder, M. E. & Deschenes, R. J. Palmitoylation: policing protein stability and traffic. Nat. Rev. Mol. Cell Biol. 8, 74–84 (2007).
Gauthier-Kemper, A. et al. Interplay between phosphorylation and palmitoylation mediates plasma membrane targeting and sorting of GAP43. Mol. Biol. Cell 25, 3284–3299 (2014).
Yount, J. S., Karssemeijer, R. A. & Hang, H. C. S-palmitoylation and ubiquitination differentially regulate interferon-induced transmembrane protein 3 (IFITM3)-mediated resistance to influenza virus. J. Biol. Chem. 287, 19631–19641 (2012).
Doubravska, L. et al. Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling. Cell Signal. 23, 837–848 (2011).
Takahashi, N. & Breitman, T. R. Retinoic acid acylation (retinoylation) of a nuclear-protein in the human acute myeloid-leukemia cell-line HL60. J. Biol. Chem. 264, 5159–5163 (1989).
Takahashi, N. & Breitman, T. R. Covalent binding of 17-beta-estradiol and retinoic acid to proteins in the human-breast cancer cell-line MCF-7. In Vitro Cell Dev. Biol. 25, 1199–1200 (1989).
Takahashi, N., Iwahori, A., Breitman, T. R. & Fukui, T. Tunicamycin in combination with retinoic acid synergistically inhibits cell growth while decreasing palmitoylation and enhancing retinoylation of proteins in the human breast cancer cell line MCF-7. Oncol. Res. 9, 527–533 (1997).
Azmi, A. S. et al. Targeting the nuclear export protein XPO1/CRM1 reverses epithelial to mesenchymal transition. Sci. Rep. 5, 13 (2015).
Kang, R. et al. Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation. Nature 456, 904–909 (2008).
Coleman, D. T., Soung, Y. H., Surh, Y. J., Cardelli, J. A. & Chung, J. Curcumin prevents palmitoylation of integrin beta 4 in breast cancer cells. PLoS ONE 10, 15 (2015).
Niu, M. S., Wu, S. J., Mao, L. & Yang, Y. L. CRM1 Is a cellular target of curcumin: new insights for the myriad of biological effects of an ancient spice. Traffic 14, 1042–1052 (2013).
Miskolci, V. et al. TNF alpha release from peripheral blood leukocytes depends on a CRM1-mediated nuclear export. Biochem. Biophys. Res. Commun. 351, 354–360 (2006).
DiSepio, D. et al. Identification and characterization of a retinoid-induced class II tumor suppressor/growth regulatory gene. Proc. Natl Acad. Sci. USA 95, 14811–14815 (1998).
Morales, M. et al. RARRES3 suppresses breast cancer lung metastasis by regulating adhesion and differentiation. EMBO Mol. Med. 6, 865–881 (2014).
Hua, S., Kittler, R. & White, K. P. Genomic antagonism between retinoic acid and estrogen signaling in breast cancer. Cell 137, 1259–1271 (2009).
Ross-Innes, C. S. et al. Cooperative interaction between retinoic acid receptor-alpha and estrogen receptor in breast cancer. Genes Dev. 24, 171–182 (2010).
Xu, X. et al. Aldehyde dehydrogenases and cancer stem cells. Cancer Lett. 369, 50–57 (2015).
Kubo, Y., Wada, M., Ohba, T. & Takahashi, N. Formation of retinoylated proteins from retinoyl-CoA in rat tissues. J. Biochem. 138, 493–500 (2005).
Minn, A. J. et al. Genes that mediate breast cancer metastasis to lung. Nature 436, 518–524 (2005).
Sevenich, L. et al. Analysis of tumour- and stroma-supplied proteolytic networks reveals a brain-metastasis-promoting role for cathepsin S. Nat. Cell Biol. 16, 876–888 (2014).
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Anderson, A., Ragan, M. Palmitoylation: a protein S-acylation with implications for breast cancer. npj Breast Cancer 2, 16028 (2016). https://doi.org/10.1038/npjbcancer.2016.28
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DOI: https://doi.org/10.1038/npjbcancer.2016.28
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