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Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain

Nature Neuroscience volume 16, pages 1370–1372 (2013)Cite this article

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Abstract

To examine the role of gray and white matter niches for oligodendrocyte differentiation, we used homo- and heterotopic transplantations into the adult mouse cerebral cortex. White matter–derived cells differentiated into mature oligodendrocytes in both niches with equal efficiency, whereas gray matter–derived cells did not. Thus, white matter promotes oligodendrocyte differentiation, and cells from this niche differentiate more easily, even in the less supportive gray matter environment.

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Figure 1: Transplantation and differentiation of adult OPCs.
Figure 2: Morphology of transplanted cells at 11 wpt.

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Acknowledgements

We thank J. Trotter (Johannes Gutenberg-University) for the NG2-EYFP and F. Kirchhoff (University of Saarland) for the PLP-DsRed mouse lines, K. Karram for advice regarding cell isolation procedures, J. Ninkovic for help with the statistics, and E. Violette and S. Sirko for carefully reading the manuscript. We are also grateful to B. Sadowski for technical help with the electron microscope, as well as the technical assistants in the Physiological Genomics. W.M. is funded by the European Research Council Advanced Investigator Grant AXOGLIA to K.-A. Nave and is supported by the Cluster of Excellence and DFG research Center Nanoscale Microscopy and Molecular Physiology of the Brain. F.V. was, in part, supported by a doctorate fellowship from the University of Milan and an International Brain Research Organization InEurope grant. This work was mainly supported by the SFB 596 and the SFB 870 of the Deutsche Forschungsgemeinschaft, the Helmholtz Association of Mental Aging and the Friedrich Bauer Stiftung.

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Authors and Affiliations

  1. Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University, Munich, Germany

    Francesca Viganò, Magdalena Götz & Leda Dimou

  2. Institute for Stem Cell Research, HelmholtzZentrum, Neuherberg, Germany

    Francesca Viganò, Magdalena Götz & Leda Dimou

  3. Department of Pharmacological Sciences, University of Milan, Milan, Italy

    Francesca Viganò

  4. Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany

    Wiebke Möbius

  5. Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany

    Wiebke Möbius

  6. Munich Cluster for Systems Neurology (SyNergy), Munich, Germany

    Magdalena Götz

Authors
  1. Francesca Viganò
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  2. Wiebke Möbius
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  3. Magdalena Götz
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  4. Leda Dimou
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Contributions

F.V. performed and designed the experiments, analyzed the data, and wrote the manuscript. W.M. performed and analyzed the immunoelectron microscopy. M.G. helped to design some experiments and wrote the manuscript. L.D. designed and supervised experiments, originally developed the transplantation technique, and wrote the manuscript. F.V., M.G. and L.D. discussed the results and implications and commented on the manuscript at all stages.

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Correspondence to Leda Dimou.

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The authors declare no competing financial interests.

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Viganò, F., Möbius, W., Götz, M. et al. Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain. Nat Neurosci 16, 1370–1372 (2013). https://doi.org/10.1038/nn.3503

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