Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Expanding the synthetic ribonucleoprotein world in cells

Protein-responsive ribozymes generated by a streamlined design process will expand the plug-and-play toolbox for synthetic biologists.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Toward programming cells.

References

  1. Ausländer, S. et al. Nat. Methods 11, 1154–1160.

  2. Win, M.N. & Smolke, C.D. Proc. Natl. Acad. Sci. USA 104, 14283–14288 (2007).

    Article  CAS  Google Scholar 

  3. Saito, H. et al. Nat. Chem. Biol. 6, 71–78 (2010).

    Article  CAS  Google Scholar 

  4. Perreault, J. et al. PLoS Comput. Biol. 7, e1002031 (2011).

    Article  CAS  Google Scholar 

  5. Ausländer, S., Ausländer, D., Müller, M., Wieland, M. & Fussenegger, M. Nature 487, 123–127 (2012).

    Article  Google Scholar 

  6. Endo, K., Stapleton, J.A., Hayashi, K., Saito, H. & Inoue, T. Nucleic Acids Res. 41, e135 (2013).

    Article  CAS  Google Scholar 

  7. Stapleton, J.A. et al. ACS Synth. Biol. 1, 83–88 (2012).

    Article  CAS  Google Scholar 

  8. Endo, K., Hayashi, K., Inoue, T. & Saito, H. Nat. Commun. 4, 2393 (2013).

    Article  Google Scholar 

  9. Kennedy, A.B., Vowles, J.V., d'Espaux, L. & Smolke, C.D. Nucleic Acids Res. doi:10.1093/nar/gku875 (1 October 2014).

  10. Warren, L. et al. Cell Stem Cell 7, 618–630 (2010).

    Article  CAS  Google Scholar 

  11. Beal, J. et al. ACS Synth. Biol. doi:10.1021/sb500173f (30 May 2014).

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hirohide Saito.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Endo, K., Parr, C. & Saito, H. Expanding the synthetic ribonucleoprotein world in cells. Nat Methods 11, 1105–1106 (2014). https://doi.org/10.1038/nmeth.3148

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.3148

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing