Riboswitches

Definition

A riboswitch is a regulatory segment within a messenger RNA (mRNA) that binds to specific metabolites and modifies the expression of the riboswitch-containing mRNA’s protein product. Riboswitches are used by bacteria to maintain specific levels of a given metabolite, by regulating the expression of the protein responsible for its accumulation.

Latest Research and Reviews

  • Research | | open

    Riboswitches are non-coding RNA elements that detect metabolites and control expression by regulating mRNA levels or translation. Here, the authors provide evidence that the E. coli thiC riboswitch has a pause site in the translation initiation region that acts as a checkpoint for thiC expression.

    • Adrien Chauvier
    • , Frédéric Picard-Jean
    • , Jean-Christophe Berger-Dancause
    • , Laurène Bastet
    • , Mohammad Reza Naghdi
    • , Audrey Dubé
    • , Pierre Turcotte
    • , Jonathan Perreault
    •  & Daniel A. Lafontaine
  • Research |

    Femtosecond XFEL crystallography is used to identify dynamic changes in the adenine riboswitch aptamer domain, with at least four states identified in real time, two in the apo form before binding and two with the ligand bound.

    • J. R. Stagno
    • , Y. Liu
    • , Y. R. Bhandari
    • , C. E. Conrad
    • , S. Panja
    • , M. Swain
    • , L. Fan
    • , G. Nelson
    • , C. Li
    • , D. R. Wendel
    • , T. A. White
    • , J. D. Coe
    • , M. O. Wiedorn
    • , J. Knoska
    • , D. Oberthuer
    • , R. A. Tuckey
    • , P. Yu
    • , M. Dyba
    • , S. G. Tarasov
    • , U. Weierstall
    • , T. D. Grant
    • , C. D. Schwieters
    • , J. Zhang
    • , A. R. Ferré-D’Amaré
    • , P. Fromme
    • , D. E. Draper
    • , M. Liang
    • , M. S. Hunter
    • , S. Boutet
    • , K. Tan
    • , X. Zuo
    • , X. Ji
    • , A. Barty
    • , N. A. Zatsepin
    • , H. N. Chapman
    • , J. C. H. Spence
    • , S. A. Woodson
    •  & Y.-X. Wang
    Nature 541, 242–246
  • Research |

    The integration of ligand-responsive riboswitches with single guide RNAs allows Cas9–effector fusions to be targeted in response to ligands and thereby translate a cellular signal into a downstream readout of choice.

    • Yuchen Liu
    • , Yonghao Zhan
    • , Zhicong Chen
    • , Anbang He
    • , Jianfa Li
    • , Hanwei Wu
    • , Li Liu
    • , Chengle Zhuang
    • , Junhao Lin
    • , Xiaoqiang Guo
    • , Qiaoxia Zhang
    • , Weiren Huang
    •  & Zhiming Cai
    Nature Methods 13, 938–944

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