Electron diffraction using three-dimensional (3D) crystals may expand the reach of this technique.
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References
Shi, D. et al. Three-dimensional electron crystallography of protein microcrystals. eLife 2, e01345 (2013).
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Doerr, A. Electron crystallography goes 3D with MicroED. Nat Methods 11, 6–7 (2014). https://doi.org/10.1038/nmeth.2797
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DOI: https://doi.org/10.1038/nmeth.2797