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Generating transgenic nematodes by bombardment and antibiotic selection

Nature Methods volume 9, pages 118–119 (2012)Cite this article

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To the Editor:

In an extension of methods we1 and others2 have previously described in Nature Methods, we report here single- or dual-antibiotic selection to isolate transgenic nematodes after microparticle bombardment. The protocol makes it straightforward to generate integrated transgenes in diverse Caenorhabditis strains and species.

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Change history

  • 27 April 2012

    In the version of this supplementary file originally posted online, there were incorrect units. Specifically, μm and μl were mistakenly converted to mm and ml. The errors have been corrected in this file as of 27 April 2012.

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Acknowledgements

This work was funded by a European Research Council Starting grant, Ministerio de Ciencia e Innovación grant BFU2008-00365, Agència de Gestió d'Ajuts Universitaris i de Recerca, the European Molecular Biology Organization Young Investigator Program, ERASysBio plus and the European Molecular Biology Center for Genomic Regulation Systems Biology Program. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the US National Institutes of Health National Center for Research Resources.

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Authors and Affiliations

  1. European Molecular Biology Laboratory Centre for Genomic Regulation Systems Biology Unit, Barcelona, Spain

    Jennifer I Semple, Laura Biondini & Ben Lehner

  2. Institució Catalana de Recerca i Estudis Avançats, Centre for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain

    Ben Lehner

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  1. Jennifer I Semple
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  2. Laura Biondini
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  3. Ben Lehner
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Correspondence to Ben Lehner.

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The authors declare no competing financial interests.

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Supplementary Figures 1–9, Supplementary Tables 1–4 and Supplementary Methods (PDF 2974 kb)

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Semple, J., Biondini, L. & Lehner, B. Generating transgenic nematodes by bombardment and antibiotic selection. Nat Methods 9, 118–119 (2012). https://doi.org/10.1038/nmeth.1864

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