Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments

Thiam, Hawa-Racine; Vargas, Pablo; Carpi, Nicolas; Crespo, Carolina Lage; Raab, Matthew; Terriac, Emmanuel; King, Megan C.; Jacobelli, Jordan; Alberts, Arthur S.; Stradal, Theresia; Lennon-Dumenil, Ana-Maria; Piel, Matthieu

doi:10.1038/ncomms10997

Download PDF

Article
Open access
Published: 15 March 2016

Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments

Hawa-Racine Thiam¹,
Pablo Vargas ORCID: orcid.org/0000-0002-6475-494X^1,2,
Nicolas Carpi¹,
Carolina Lage Crespo³,
Matthew Raab¹,
Emmanuel Terriac¹,
Megan C. King⁴,
Jordan Jacobelli⁵,
Arthur S. Alberts⁶,
Theresia Stradal⁷,
Ana-Maria Lennon-Dumenil² &
…
Matthieu Piel¹

Nature Communications volume 7, Article number: 10997 (2016) Cite this article

16k Accesses
219 Citations
14 Altmetric
Metrics details

Subjects

Abstract

Cell migration has two opposite faces: although necessary for physiological processes such as immune responses, it can also have detrimental effects by enabling metastatic cells to invade new organs. In vivo, migration occurs in complex environments and often requires a high cellular deformability, a property limited by the cell nucleus. Here we show that dendritic cells, the sentinels of the immune system, possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. The cells’ requirement for Arp2/3 to pass through constrictions can be relieved when nuclear stiffness is decreased by suppressing lamin A/C expression. We propose a new role for Arp2/3 in three-dimensional cell migration, allowing fast-moving cells such as leukocytes to rapidly and efficiently migrate through narrow gaps, a process probably important for their function.

Optogenetic control of mRNA condensation reveals an intimate link between condensate material properties and functions

Article Open access 15 April 2024

Microenvironmental reorganization in brain tumors following radiotherapy and recurrence revealed by hyperplexed immunofluorescence imaging

Article Open access 15 April 2024

Spatially organized cellular communities form the developing human heart

Article Open access 13 March 2024

Introduction

The actomyosin machinery required for cell locomotion has been described in great details¹. It is mainly based on actin treadmilling and actomyosin contraction^2,3. The net rearward movement of actin filaments is transferred to the migration substratum via specific adhesion proteins (for example, integrin receptors^4,5), leading to forward cell locomotion^1,6. This well-defined set of physico-chemical processes, initially described based on cultured cells moving on flat glass surfaces, seems to be universal among migrating cells. Nevertheless, recent studies performed in more complex environments have proposed alternative scenarios and additional requirements for cell locomotion, suggesting that the precise function of some proteins might depend on the environment of migrating cells. The confining nature of micro-channels, gels of the extracellular matrix or tissues can relieve the need for specific integrin-based adhesion in immune and cancer cells^7,8,9,10. The complexity of three-dimensional (3D) gels also requires cells to restrict the number of their protrusions, to avoid simultaneously engaging multiple paths. This constraint makes polarity proteins such as Cdc42 essential for 3D locomotion, whereas on two-dimensional substrates it is required only for directionality¹¹.

In addition, the porous nature of gels and tissues places a strong requirement on cell deformability/compliance¹². Owing to the dynamic nature of the actin and microtubule cytoskeletons, the high compliance of the plasma membrane and of the majority of membranous internal organelles, most cytoplasmic elements do not limit cell deformation through the micrometric pores found in tissues¹³, at least on the time scale of cell locomotion. Cytoplasmic intermediate filaments can limit cell deformation^14,15; however, several studies have shown that the element that usually limits deformation through narrow pores is the cell nucleus^16,17. Indeed, the nucleus has a specific set of intermediate filaments that form a rigid shell underneath the inner nuclear membrane; these filaments are encoded by the genes for lamin A/C and B (LMNA and LMNB1/2).

To overcome this limitation, some migrating cells express a low level of lamin gene products, whereas others secrete proteases to enlarge pores in the extracellular matrix¹⁸. Matrix degradation is important for the migration of metastatic cells, but does not seem to be required for immune cell migration^17,19. Although the degree of lamin A/C expression correlates with the capacity of cells to pass through small pores in a protease-independent manner^16,17,20, low levels of lamin A/C also correlate with lower cell viability¹⁶. Immune cells such as neutrophils do not express lamin A/C and have a short lifespan (dying at the site of infection); however, dendritic cells (DCs), which patrol peripheral tissues and present antigens to T cells in lymph nodes, need to combine a high migration capacity and long-term survival, a combination which might also be essential for the metastatic capability of some cancer cells¹⁶.

We here aimed at studying the mechanism used by DCs to deform and migrate through narrow pores. Our study reveals that, as already reported for various other cell lines^16,17,21,22 the nucleus limits confined 3D migration in DCs. We show that DCs overcome this physical limitation by generating a dense and dynamic perinuclear actin network nucleated by Arp2/3 downstream of Wave2. This Arp2/3-nucleated perinuclear actin network allows nuclear deformation and the subsequent cell passage through constrictions, probably by rupturing the intranuclear lamina shell.

Results

The nucleus limits DC migration through micrometric pores

Mouse bone-marrow-derived DCs express higher levels of lamin A/C proteins compared with neutrophils¹⁷ or B lymphocytes (Supplementary Fig. 1A,B). However, they have a lower expression level of lamin A/C proteins than mouse embryonic fibroblasts (Supplementary Fig. 1A,B) or cultured cells used as model systems for tumour invasion (fibrosarcoma cells HT1080 and breast adenocarcinoma MDA-MB231; Supplementary Fig. 1A,B), which are slow-migrating cells that require matrix proteases for migration in dense 3D collagen gels¹⁷. DCs display an intermediate level of lamin A/C expression, consistent with a double requirement for a longer lifespan and a high migratory capacity independent of proteases¹⁹. This raises the question of how such lamin A/C-expressing cells deform their nucleus through small pores. Actomyosin contraction was proposed to play such a role, generating a pressure at the cell back that pushes the nucleus forward^23,24. Myosin II is also required for cell locomotion per se^17,25,26, and for cell polarity and retraction of lateral protrusions²⁷, making it difficult to clearly identify a specific role for it in nuclear deformation.

To ask how DCs deform their nucleus, we studied their migration through microfabricated constrictions²⁸ of well-defined sizes coated with Fibronectin (Fig. 1a,b and Supplementary Figs 1C,D and 2A,B; for ranges of physiological pore sizes see refs 13, 17). DCs migrated spontaneously and at high speed (mean velocity of 4.48±3.93 μm min⁻¹; mean±s.d.; n>100, N=2) in 7-μm-wide channels devoid of constrictions (see Supplementary Table 1 for all channels and constriction dimensions). Constrictions ≥1.5 μm in width induced a strong nuclear deformation but did not prevent cell passage (Fig. 1c–e, Supplementary Figs 1E–G and 2C,D, and Supplementary Movie 1; see also Supplementary Table 2 for exact mean±s.e.m. and P-value of all quantifications).

**Figure 1: The nucleus imposes a physical limitation to DC migration through micrometric pores.**

Time-lapse recording of migrating cells identified four phases of transmigration (Fig. 1d) as follows: (i) cell front entry into the constriction, which did not induce any slowdown; (ii) nuclear engagement and deformation, which led to a strong slowdown in constrictions <3 μm in width (Fig. 1f–h); (iii) nuclear exit; and (iv) cell exit. Varying the length of the constriction, from 15 μm to either 20 μm or 5 μm, did not significantly affect cell passage (compare Fig. 1 and Supplementary Fig. 1 with Supplementary Fig. 2). However, there was a clear effect of the constriction width (Fig. 1e–h) as previously reported in gels¹⁷. At 1 μm width, the cell front passed but only a small fraction of the nucleus was engaged in the constriction (Supplementary Fig. 1E and Supplementary Movie 1), leading to a very low (4%) rate of cell passage. Increasing the constriction width to 1.5 μm allowed 40% of cells to pass (Fig. 1e, Supplementary Fig. 1F and Supplementary Movie 1). The passage rate reached a maximum at ≥3 μm (78%; Fig. 1e and Supplementary Fig. 2D).

Quantifications (Fig. 1e–g and Supplementary Fig. 2D,E) showed that for intermediate sizes of 1.5 and 2 μm, while a majority of cells could pass through the constriction, nuclear passage induced a significant reduction of cell velocity (41% in 1.5 μm constrictions). Despite that delay, DCs passed through such small constrictions in only ∼20 min, while metastatic cells or fibroblasts have been reported to take several hours to pass through similar constrictions (mouse embryonic fibroblasts²¹ and MDA-MB-231 (ref. 22)). This suggests that fast immune cells might have a specific mechanism for deforming their nucleus in a shorter time. Importantly, for all sizes, non-passing DCs engaged in a constriction spent at least as much time in the constriction as passing cells (Supplementary Figs 1H and 2F), showing that non-passage was not due to an early change of direction of the cell induced by the constriction. Thus, DCs, despite their ability to express lamin A/C, pass constrictions down to ∼1.5 μm in width at high velocity. However, below 3 μm the DC nucleus is limiting their passage and speed.

Arp2/3 is required for DC passage through micrometric pores

To identify cytoskeletal effectors essential for nuclear passage, we performed a candidate screen using 2-μm-wide constrictions (2 × 3.5 μm²). Disrupting microtubules with nocodazole (Supplementary Fig. 3A) increased cell passage rate (Fig. 2a) and reduced passage time (Fig. 2b). Depletion of mDia1, the most expressed formin in DCs (Immunological Genome project http://www.immgen.org), which has been implicated in DC migration²⁹, only slightly reduced immature DC speed in channels without constrictions (Fig. 2c, see also³⁰) but had no effect on the rate of passage (Fig. 2a). Surprisingly, myosin II inhibition or depletion of myosin IIA (the only isoform expressed in DCs, Immunological Genome project and Supplementary Fig. 4A) had no effect on the rate of passage through constrictions (Fig. 2a and Supplementary Fig. 4B,C), even though, as expected, it had a strong effect on cell shape (Supplementary Fig. 4A,B), cell speed (Fig. 2c) and thus passage time (Fig. 2b and Supplementary Fig. 4D). We conclude that myosin II plays a role in increasing cell speed but is not required in DCs for nuclear deformation through small constrictions.

**Figure 2: Arp2/3-based actin polymerization is required for DC passage through constrictions.**

High doses of latrunculin A (250 nM), which depolymerizes actin filaments (Supplementary Fig. 3B), completely stopped cell migration (Supplementary Movie 2), ruling out an osmotically driven migration mechanism³¹. At low doses (50 nM), latrunculin A did not induce obvious changes in the overall actin cytoskeleton (Supplementary Fig. 3C and Supplementary Movie 2) neither slowed down cell movement in channels (Fig. 2c), but it prevented nuclear passage through constrictions (Fig. 2a). A similar result was obtained when the actin nucleator Arp2/3 was inhibited by CK666 (ref. 32), depleted by small interfering RNA (siRNA) (Supplementary Fig. 5A) or when the haematopoietic specific Wave2 subunit Hem1 was depleted³³ (Fig. 2a–c). The reduction in passage rate through constrictions was not due to an increase in changes of direction, as treated/depleted cells spent at least the same amount of time in constrictions as control/wild-type (WT) cells did (Fig. 2d), and they initiated nuclear entry into constrictions to the same extent as non-passing control/WT cells (Fig. 2e). Arp2/3 inhibition reduced the rate of passage only for constrictions <3 μm (Supplementary Fig. 5B,C), a result consistent with a specific function of Arp2/3 in nuclear passage. Overall, this screen revealed that Wave2/Arp2/3-based actin nucleation is specifically needed in DCs for nuclear passage through small constrictions.

Actin accumulates in constriction during nuclear passage

The requirement for Arp2/3 prompted us to look in more detail at the localization of actin filaments in cells passing through constrictions. DCs derived from LifeAct-GFP transgenic mice³⁴ showed a very strong enrichment of actin filaments around the nucleus inside small constrictions (Fig. 3a, Supplementary Fig. 6B and Supplementary Movie 3). This was confirmed with phalloidin staining of fixed cells (Supplementary Fig. 6A) and was quantified in live cells, showing that it was temporally restricted to the time during which the nucleus was deformed in the constriction and spatially limited to the constriction (Fig. 3b,c, Supplementary Fig. 6C and Supplementary Movies 3 and 4). This enrichment of actin was specific to small constrictions (Supplementary Fig. 6 C–N and Supplementary Movie 4) that required Arp2/3 for efficient passage (Fig. 2a and Supplementary Fig. 5B, C). This enrichment was distinct and stronger than the permanent enrichment of actin at the cell back and the transient enrichments observed at the cell front (yellow and pink arrow heads in Supplementary Fig. 6B,E, respectively). Actin accumulation around the nucleus was also observed in non-adhesive (pLL-g-PEG coated) channels (Supplementary Fig. 7A,B and Supplementary Movie 5) ruling out the involvement of adhesion complexes. Imaging of actin filaments in DCs migrating in dense, 3 mg mL⁻¹ rat tail collagen gels¹⁷ (Fig. 3d) or in mouse ear explants (Supplementary Movie 6), and also in myeloid cells migrating in live Medaka fish, showed that the degree of nuclear deformation correlated with an accumulation of actin around the nucleus (Fig. 3e,f and Supplementary Movie 7). This suggests that a specific set of actin filaments is assembled around the nucleus of a DC when it is strongly confined in vitro and in vivo. We propose to name this network confinement induced actin network (CiAN).

**Figure 3: A dynamic actin meshwork forms around the nucleus and deform this organelle during its passage through narrow constrictions.**

CiAN induces local nuclear deformation

To assess where forces were exerted during nuclear passage, we recorded cells passing through a constriction by reflection interference contrast microscopy. We found that cells were in close contact with the channel walls only at the rear and inside the constriction, but that the front part of the cell, which passed the constriction before the nucleus, had almost no contact with the bottom wall of the channel (Supplementary Fig. 7C and Supplementary Movie 8). This result, together with the absence of requirement for adhesion, suggests that forces exerted on the nucleus could not be pulling forces from the front protrusion, contrary to what was suggested by experiments performed on slow migrating mesenchymal cells³⁵. They could instead be either pushing forces from the back or lateral forces in the constriction. To test this hypothesis, we measured, all along constrictions, both the local width and the local density of the chromatin staining, as a function of the density of the local LifeAct-GFP staining. This showed that a local higher density of actin filaments correlated with a locally thinner and denser nucleus (smaller than the actual constriction width) (Fig. 3g–i). Overall, these data suggest that actin filaments accumulated around the nucleus in the constriction exert a lateral pushing force, increasing its deformation.

CiAN facilitates nuclear passage

This result prompted us to record the perinuclear actin network in non-passing cells. More than 90% of control cells passing through constrictions displayed an actin accumulation around the nucleus versus <30% of non-passing cells that engaged their nucleus in the constriction (Fig. 4a–c). Interestingly, 58% of cells that did not form a perinuclear actin network at the moment of nuclear entry displayed a higher actin density in constriction before nuclear entry (Fig. 4b). This subpopulation of cells nucleating actin in constrictions before nuclear entry was never observed in the 88 analysed passing cells. The remaining 42% did not show an increase in actin density in constriction neither prior nor when the nucleus was inside the constriction. When measuring the density of actin around the nucleus over time, we found that non-passing cells had in average a lower density of perinuclear actin network than passing cells (Fig. 4c; it is noteworthy that the amount of perinuclear actin in non-passing cells was increasing for nuclei, which reached further inside the constriction). These results suggest that cells that did not pass constrictions either did not assemble actin around their nucleus but in front of it, or assembled actin at a lower rate and in average up to a lower level than passing cells.

**Figure 4: The dynamic perinuclear actin network is nucleated by Arp2/3.**

We then recorded actin density around the nucleus in cells treated with 50 nM latrunculin A (Supplementary Fig. 8 and Supplementary Movie 9). We found that the density of perinuclear actin increased at a much lower rate and up to a lower maximal density than in control cells (Fig. 4d; it is noteworthy that even for treated cells the amount of actin increased for nuclei that reached further in the constriction), leading to a lower average actin accumulation inside constrictions (Fig. 4e). We also observed two patterns of actin accumulation around or in front of the nucleus in latrunculin A-treated cells that failed to pass constrictions (Supplementary Fig. 8B,C). Together, these results suggest that the amount of actin accumulation around the nucleus inside constrictions is strongly correlated with the fate of the cell (passing or not). This is consistent with a role for perinuclear actin in helping nuclear passage through the constriction.

Arp2/3 mediates CiAN assembly while myosin II does not

As Arp2/3 complex depletion/inhibition had a strong effect on nuclear passage, we tested whether it was responsible for the assembly of the perinuclear actin network. We immunostained Arp3 in cells fixed inside constrictions, after removal of the confining polydimethylsiloxane (PDMS) layer, to allow a direct access for the antibody to the cells (see Methods). This revealed a clear enrichment of the Arp2/3 complex around the nucleus, co-localized with the enrichment in actin filaments stained by phalloidin (Fig. 4f and Supplementary Fig. 9A; n=10). Of cells that passed through constrictions after Arp2/3 inhibition, only a minor fraction still displayed an actin accumulation around the nucleus (Fig. 4a,g, Supplementary Fig. 9B,C and Supplementary Movie 10). These results suggest that Arp2/3 might be responsible for the increase in actin density around the nucleus of cells passing constrictions.

Consistent with a requirement for myosin II for cell speed and not for nuclear passage, myosin IIA-GFP, imaged in DCs derived from myosin IIA-GFP transgenic mice³⁶, was strongly enriched at the cell back when cells were passing constrictions and did not show any enrichment around the nucleus inside the constriction (Fig. 4h,i, Supplementary Fig. 10A and Supplementary Movie 11). Moreover, inhibition of myosin II with blebbistatin did not prevent actin accumulation around the nucleus in constrictions (Supplementary Fig. 10B; representative of 19 over 21 observed cells), whereas it completely inhibited myosin II accumulation at the cell rear (Supplementary Fig. 10C; n=30; N=2). These results suggest that the actin accumulation observed around the nucleus in constrictions relies on Arp2/3 activity and corresponds to accumulation of newly polymerized actin filaments rather than to a contractile acto-myosin ring.

Thus, in DCs, myosin II motor activity, but not Arp2/3-based actin nucleation, is required for fast locomotion^24,30, probably by increasing the rear contractility of the cell, whereas Arp2/3 actin nucleation around the nucleus, but not myosin II motor activity, allows efficient passage through small constrictions, probably by generating a lateral pressure on the nucleus.

Nuclear passage leads to transient nuclear lamina rupture

To further understand how actin nucleation around the nucleus helps nuclear deformation, we stained lamin A/C and lamin B in fixed cells inside constrictions. The perinuclear lamina staining was interrupted or fragmented (Fig. 5a,b and Supplementary Fig. 11A–C) in 87% of the 98 observed cells with nuclei engaged in 2-μm-wide and 15-μm-long constrictions, whereas such staining was observed in only 7% of the 142 cells observed in straight, 8-μm-wide channels and was never observed in the 177 cells observed outside of channels (Fig. 5c,d). Only 43% of the cells engaged in 2-μm-wide but 5-μm-long constrictions showed a ruptured lamina network (Fig. 5d and Supplementary Fig. 11D), suggesting that increased ruptures correlate with increased global nuclear deformation (probably due to the increase in total surface area) and not only with the local minimal width of the constriction.

**Figure 5: Arp2/3-nucleated perinuclear actin facilitates nuclear squeezing through constrictions by weakening the lamin A/C network.**

In 97% of cells, the ruptured region was located at the nuclear tip (Fig. 5a–e and Supplementary Fig. 10), with part of the chromatin extruding out of the nuclear lamina staining, consistent with what was observed when cells were externally compressed³⁷. These data suggest that the accumulation of actin filaments might be required to deform the lamina network, possibly by exerting pressure on it³⁸, leading to its rupture at the site of highest curvature at the nuclear tip. Overall, these results suggest that on nuclear deformation, the nuclear lamina ruptures, which might facilitate the passage of the nucleus.

To directly test this hypothesis, we depleted lamin A/C in DCs (Supplementary Fig. 12) and inhibited Arp2/3. In contrast to control cells, inhibition of Arp2/3 did not prevent passage through small constrictions in lamin A/C-depleted cells (Fig. 5f). Depletion of lamin A/C did not affect the time required for passage through constrictions (Fig. 5g). These results show that Arp2/3-dependent actin nucleation around the nucleus is required to pass through small constrictions only in lamin A/C-expressing cells and they suggest that its function might be to facilitate nuclear deformation through transient rupture or disassembly of the lamin A/C network.

The requirement for a branched actin network (nucleated by Arp2/3 rather than formins) might be linked to the capacity of such networks to exert strong pressure via an increase in the density of branches when growing under a load or in confinement^{39,40,41,42,43}. Building on this concept, we propose that the accumulation of actin around the nucleus could be simply induced by the high load produced by the stiff nuclear lamin A/C mesh when it is confined within the constriction. Consistent with this proposal, we found that actin filament accumulation around the passing nuclei was reduced in cells depleted for lamin A/C (Fig. 5h,i). This suggests that the presence of the nuclear lamina not only makes Arp2/3 and perinuclear actin essential for nuclear passage, but is also responsible for triggering the formation of the perinuclear actin network on nuclear passage through a constriction.

CiAN does not depend on the LINC or on the nucleus

To test whether actin accumulation was due to a specific pathway associated with the presence of the nucleus in the constriction, we performed two sets of experiments. First, we inhibited the LINC complex, which is known to physically link the nuclear periphery to the actin cytoskeleton (for a recent review, see ref. 44). We chose to deplete the SUN proteins^45,46. We used cells from a SUN2KO mice⁴⁷ and RNA interference depletion of Sun1 (Supplementary Fig. 13A,B). As expected, we observed that LINC-depleted cells were less persistent and, consistently, have a lower non-passage time (Supplementary Fig. 13C–E). Nevertheless, we did not observe any effect on the percentage of cell passage through 2-μm-wide constrictions (Fig. 6a and Supplementary Fig. 13F) and actin accumulation was still observed around the passing nuclei (Fig. 6b representative of 16 over 20 cells, N=2). This suggests that the LINC complex is necessary neither for nuclear passage nor for the induction of actin polymerization on nuclear entry in the constriction.

**Figure 6: Actin assembly inside constrictions is not specific to the nucleus and can be induced by confinement of rigid particles.**

Second, we reasoned that if actin nucleation was simply induced by confinement of the Arp2/3-nucleated network by the stiff nucleus, any stiff object inside the cell should have the same effect. We took advantage of the capacity of DCs to internalize large particles²⁴ and incubated cells with polystyrene beads of 3 μm of diameter. The migration of LifeAct-GFP-expressing DCs loaded with such beads through 3.5-, 3- and 2.5-μm-wide constrictions was then recorded (Supplementary Movie 12). In more than 80% of cases, actin accumulated around 3 μm beads when they passed through 3 μm constrictions (Fig. 6c–e and Supplementary Movie 12). In 2.5 μm constriction, no beads could pass (beads remained stuck at the constriction entry) and no actin was observed around these beads, showing that entry inside the constriction was required to trigger actin accumulation (Fig. 6e). Although in this range of sizes (2.5–3.5 μm) the rate of nuclear passage did not change, the rate of cell passage was correlated to the rate of bead passage through the constrictions (Fig. 6e), showing that beads were then the limiting factor for cell passage. In 3.5 μm constrictions, no actin accumulation was observed around the beads (Fig. 6e–h). No correlation was observed between bead velocity and the measured actin density around beads, ruling out a requirement for shear stress on actin accumulation in constrictions (Fig. 6i). Moreover, when cells were loaded with both 2 μm and 3 μm beads, actin accumulation was observed only around 3 μm beads that entered the 3 μm wide constrictions (Supplementary Movie 13). These experiments show that stiff objects that are confined in constrictions, that is, beads that match the constriction size, can induce the accumulation of actin to the same extent as the nucleus. Together, these data strongly suggest that actin accumulation around the nucleus in constrictions is caused by confinement due to nuclear stiffness and rule out the requirement for specific nucleus-associated components.

Discussion

Our results identify a new mechanism required for the migration of fast-moving lamin A/C-expressing cells through small constrictions. This mechanism involves the nucleation of an Arp2/3-dependent actin network around the nucleus, which would transiently weaken the lamin A/C nuclear shell, making the nucleus more deformable. A transient rupture of the lamin A/C intranuclear shell, followed by re-assembly, has already been documented in the case of an imposed nuclear deformation³⁷, suggesting that strong pushing forces could rupture the nuclear lamina without killing the cell. Arp2/3 actin nucleation was also found to facilitate the nuclear envelope rupture at mitotic entry in starfish oocytes⁴⁸, a phenomenon also associated with nuclear lamina disassembly. The precise mechanism by which actin assembly is triggered and the nuclear lamina shell deformed will require further study (see the Supplementary Discussion section on other actin structures recently reported to be associated to the cell nucleus^{49,50,51,52,53,54,55}).

The LINC complex, which binds both the nuclear lamina and the cytoskeleton, is not required for nuclear passage through micrometric pores (see also the Supplementary Discussion section). As shown for other processes such as nuclear envelope breakdown⁵⁶ and the adaptation of nuclear stiffness to tissue mechanical properties³⁸, lamin A/C assembly might be regulated by phosphorylation on nuclear deformation. A recent article indeed suggested that ATR, a kinase normally involved in DNA repair, was activated and localized to the nuclear periphery on mechanical perturbation of the nucleus⁵⁷. Nevertheless, our observation of very local ruptures of the lamin A/C shell rather suggests a mechanical rupture than a signalling event.

According to our results, other cell types that do not express lamin A/C and thus have a softer nucleus should not show perinuclear actin accumulation. To test this idea, we performed a similar set of experiments on HL60-derived neutrophils, which have a low lamin A/C expression level²⁰ (Supplementary Fig. 1A,B). These cells could pass through 1 μm constrictions (Supplementary Fig. 14A–C), which WT DCs cannot pass, consistent with a softer nucleus²⁰. Similar to DCs depleted in lamin A/C, they did not need Arp2/3 to pass small constrictions (Supplementary Fig. 14D,E). No actin accumulation was observed around the nucleus of HL60-derived neutrophils even in the smallest constrictions (Supplementary Fig. 14A,F), with actin being rather enriched at the cell back (Supplementary Fig. 14A). Overall, these results confirmed that Arp2/3-dependent actin nucleation around the nucleus is required only for the deformation of nuclei, which possess a lamin A/C network. They also confirm that accumulation of perinuclear actin filaments depends on the presence of a lamin A/C intranuclear shell.

A surprising result from our study is that myosin II, while increasing the speed of cells through constrictions, was not required for the passage itself. This was also the case for cells after lamin A/C knockdown (Fig. 5f) and for neutrophils when passing similar constriction sizes (Supplementary Fig. 14G,H). Nevertheless, myosin II activity was required for neutrophils to pass through the smallest constrictions (1 μm), DCs not being able to pass such constriction (Supplementary Fig. 14D,E,G,H). In this case, in the absence of a nuclear lamina shell the resisting element might be the chromatin itself, which is known to have a high viscosity⁵⁸. myosin II contractility at the cell rear could then become necessary, to produce enough pressure to allow a flow of this viscous material through such a narrow pore. The mechanism we describe here allows fast migration through small constriction, by combining myosin II-dependant rear contraction and Arp2/3-dependant lateral compression of the nucleus (see the Supplementary Discussion section on the difference between mesenchymal and amoeboid cells).

Altogether, our results are in qualitative agreement with the following model (Supplementary Fig. 15): when cells face small constrictions, forces generated by the migration apparatus are enough to push the nucleus against the constriction, leading to the insertion of a part of the nucleus, whose size depends on the constriction width, the nuclear stiffness and the pushing force. The part of the nucleus inserted in the constrictions triggers the accumulation of Arp2/3-nucleated actin, due to confinement of the network^43,59. This actin network produces a lateral compression force, which further deforms the part of the nucleus inserted in the constriction, allowing further entry. When nuclear surface deformation reaches a certain threshold, the nuclear lamina locally ruptures, allowing full passage of the nucleus, at a rate set by the pushing force, the nuclear viscosity and the constriction cross-section area. In-depth physical modelling and quantitative biophysical experiments will be necessary to further test this model.

The fact that CK666 treatment reduces the percentage of cells that pass through the constrictions (Fig. 2a) but not the passage time (Fig. 2b) might seem surprising. Nevertheless, in the frame of our working model, this is perfectly consistent. The lateral compression by the Arp2/3-nucleated actin does not produce any forward pushing force; it is fighting against the energy barrier set by the stiffness of the elastic nuclear lamina. Thus, this contribution of Arp2/3-nucleated actin sets the level of deformation that can be reached. On the contrary, the cortex tension imposed by myosin II motors sets the difference of pressure between the front and the back of the nucleus when it is obstructing the constriction. This difference of pressure sets the time it will take, for a given viscosity, to pass through a constriction of a given size. In conclusion, myosin II motor activity is related to the viscous part of the nuclear resistance and this sets the passage time for a given channel size, whereas Arp2/3-nucleated actin is related to the elastic part of the nuclear resistance and thus sets the fraction of passing cells for a given amount of deformation of the nuclear surface required for passage. Importantly, such reasoning also suggests that depending on the visco-elastic properties of their nucleus and their migration speed, various types of cells might fall either in a range of parameters in which the elastic properties of their nucleus dominates, making Arp2/3-nucleated actin essential, or in a range of parameters in which the nucleus is rather viscous, in which case Arp2/3 will not be required and myosin II activity will set the passage time. As most visco-elastic materials are viscous at long time scales, a prediction of that model is that slow migrating cells, just similar to cells that do not express lamin A/C, will not require Arp2/3 for nuclear passage.

In conclusion, we show that DCs possess a specific mechanism, based on Wave2/Arp2/3 actin nucleation around the nucleus that enables them to deform their nucleus despite the presence of a stiff lamin A/C intranuclear shell. We propose that this mechanism helps them combine a high migration speed and a high level of deformability, with long-term survival. These characteristics are required for DCs to achieve their functions from peripheral tissue sampling to antigen presentation in lymph nodes. We anticipate that this mechanism might be used by other long-lasting leukocytes, and that it could facilitate the migration and survival of fast amoeboid metastatic cells through dense matrices and tissues.

Methods

Mice

Myosin IIA knockout mice were generated by crossing MyoIIAflox/flox mice¹⁰ with CD11c-Cre mice⁶⁰ and with Gt(ROSA)26Sor–flox-stop-flox–YFP mice (Rosa-YFP)⁶¹, to obtain MyoIIAflox/flox-CD11c-Cre+-Rosa-YFP mice that were used as bone marrow donors. Littermates or age-matched MyoIIAwt/wt-CD11c-Cre+–Rosa-YFP mice were used as control bone marrow donor mice. The breeder mice were previously backcrossed to C57BL/6 for at least seven generations.

I-Aβ^b-GFP and Myosin IIA-GFP knock-in mice were previously described^36,62.

Lifeact-GFP mice were a kind gift from Michael Sixt lab (IST, Austria) and were generated as described³⁴.

Details of the generation of the Hem1 knockout mice are described in ref. 33. Briefly, exons 4 and 5 were targeted and flanked with loxP sites and deletion of Hem1 was induced by crossing the mouse with a mouse expessing Cre recombinase under control of the keratin 14 promoter. Cells from Hem1KO mice (Stradal Lab) were kindly provided by Michael Sixt.

mDia1 knockout mice were obtained from Arthur Alberts lab (Van Andel Institute, USA) and were generated as described in ref. 63.

Sun 2 knockout mice previously described in ref. 47 were obtained from Megan King’s lab (Yale School of Medicine, USA).

C57BL/6 mice, described in ref. 25, were used for immunofluorescence (in Figs 4c and 5a,b, and Supplementary Figs 3A,5D,6A and 11A). Each result presented here was obtained from two to four independent experiments (two to four different mice). The experiments were performed on 6-week-old male or female mice. For animal care, we strictly followed the European and French National Regulation for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Directive 2010/63; French Decree 2013-118). The present experiments, which used mouse strains displaying non-harmful phenotypes, did not require a project authorization and benefited from guidance of the Animal Welfare Body, Research Centre, Institut Curie.

Cells

Immature mouse bone-marrow-derived DCs were cultured 10–12 days in DC medium (IMDM, FCS (10%), Glutamine (20 mM), pen–strep (100 U ml⁻¹) and 2-ME (50 μM)) supplemented with granulocyte–macrophage colony stimulating factor (50 ng ml⁻¹)-containing supernatant obtained from transfected J558 cells, as previously described²⁵.

To generate neutrophils, HL60 cells were maintained in culture and differentiated with 1.3% of DMSO as previously described in ref. 64. Lyn-emerald/Utrophin-mCherry HL60 was a kind gift from Dyche Mullins lab (UCSF, USA) and actin-GFP HL60 was kindly provided by Guillaume Charras (UCL, UK).

Antibodies and reagents

For imaging the nucleus, cells were incubated with 200 ng ml⁻¹ of Hoechst 33342 (Life Technologies) or 34580 (Invitrogen) for 30 min at 37 °C and 5% CO₂.

The following antibodies were used for immunoblotting: LmnA/C (H110, Santa Cruz), anti-ArpC4 and anti-sun2 (Abcam), and anti-actin (Millipore). Antibodies were used at 1:1,000 dilution.

For immunofluorescence, we used Alexa Fluor 594-coupled phalloidin (Invitrogen; 1:400), anti-LmnAC (N18, Santa Cruz (1:50) and clone 4C11 from Sigma Aldrich (1:2,000)), anti-Lamin B1 (ab16048, Abcam (1:500)) and anti-Arp3 (Abcam; 1:200); DAPI (4,6-diamidino-2-phenylindole) for DNA staining; and secondary antibodies anti-mouse-Alexa488 and anti-Goat-Alexa488 from Jackson ImmunoResearch Laboratories were used at 1:400. Slides were mounted with custom-made Moviol.

For lymphatic vessels visualization, anti-LYVE-1 (R&D System, 1/1,000) was used.

For drug treatment CK666, latrunculin A and blebbistatin were obtained from Tocris Bioscience, Y27632 from Calbiochem, nocodazole and DMSO from Sigma-Aldrich. For silencing, Smart pool ON-TARGETplus mouse LMNA siRNA, Arpc4 siRNA and Sun1 siRNA were purchased from Thermo Fisher Scientific. Micro-channels were coated with fibronectin (Sigma) or custom-made pLL(20)-g[3.5]-PEG(2)-Rhodamin.

siRNA silencing

DCs were transfected with SMARTpool: ON-TARGETplus siRNAs specific for Arpc4, LMNA or Sun1 (Thermo scientific) using the Amaxa Mouse Dendritic Cell Nucleofector Kit (Lonza). Briefly, 3 × 10⁶ DCs collected at day 7 of differentiation were transfected in 100 μl of Amaxa solution containing 1 μM of the desired siRNA. Transfected cells were cultured during 48–72 h in DC medium and then used to perform the desired experiment.

We noted that systematically, and for unknown reasons, control siRNA-electroporated DCs migrate faster than non-electroporated ones. Thus, they have a higher rate of passage through constrictions and a lower passage time (Supplementary Fig. 2 compared with Supplementary Fig. 12). For this reason, values should be compared with their internal control (either non-electroporated or electroporated).

Beads internalization

DCs were concentrated at 2 × 10⁷ cells per ml and incubated with 3 μm polystyrene beads (17145-5, amino beads, Polysciences) at 2:1 beads per cells ratio for 1 h at 37 °C/5% CO₂. Cells were resuspended in 1 ml of PBS (Gibco), which is then deposited on 3 ml of FCS before being centrifuged at 900 r.p.m. for 5 min, allowing the removal of floating non-internalized beads.

Channels preparation and cell loading

Microchannels were prepared as previously described^25,28. Briefly, PDMS (GE Silicones) was used to prepare 7 × 5 μm microchannels with constrictions from a custom-made mould. The PDMS chamber and a 35-mm glass-bottom dish (FD35-100, WPI) were plasma activated before being bound to each other. The binding was left to strengthen in a 65 °C oven for 1 h. The so-obtained microchannels were plasma cleaned then incubated with 50 μg ml⁻¹ of fibronectin or 100 μg ml⁻¹ of pLL-PEG-Rhodamin at RT for 1 h then washed with PBS before being incubated with the adequate medium (containing drugs if necessary) for at least 1 h at 37 °C and 5% CO₂ before cell loading.

Drugs treatment

CK666 was used at 50 μM, latrunculin A at 50, 100 and 250 nM, blebbistatin at 50 μM, Y27632 at 30 μM for DCs and 10 μM for HL60-derived neutrophils, nocodazole at 10 μM and DMSO was used as control for all drugs, except Y27632, which was dissolved in water. When drugs were used in microchannel experiments, the chamber was incubated with drug containing medium for at least 1 h before cell loading. Cells were resuspended in drug containing medium before being deposited in the chamber entry pores.

Immunoblotting

DCs were lysed on ice for 45–60 min in a buffer containing either 50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS (Supplementary Fig. 1A,B) or 100 mM Tris, 150 mM NaCl, 0.5% NP-40 (Supplementary Figs 5,12 and 13) or 1:100 of protease inhibitor cocktail (Roche) and 1:100 of phosphatase inhibitor cocktail (Sigma). Protein concentration was quantified using the DC Protein Assay (Biorad). Cell lysate was suspended in 2 × loading buffer containing 4% SDS. Thirty micrograms of soluble extracts were loaded onto a 4–20%/4–12% TGX gradient gel (BioRad) and transferred onto a Trans-Blot Turbo PVDF membrane (BioRad). The membrane was blocked using in 1 × Tris-Buffered saline solution containing 0.1% of Tween-20 and 5% of Milk, incubated with the appropriate antibodies and revealed with SuperSignal West Dura substrate (Thermo Scientific).

Quantitative PCR

Cells were cultured as described above. RNA extractions were performed using NucleoSpin RNA (Macherey-Nagel), according to manufacturer’s protocol. Complementary DNA were obtained with SuperScriptVILO cDNA synthesis kit (Life Technologies), according to manufacturer’s protocol, starting from 1 μg of RNA. Quantitative PCR were done with the Lightcycler 480 (Roche) using Taqman Gene expression assay (Applied Biosystem) with the following primers: Mm00659179_m1 (best coverage primers for mouse Sun1) and Mm99999915_g1 (gapdh, as a control).

Video microscopy and quantification of cell passage through constrictions

Migrating cells were imaged for 15–20 h on an epifluorescence microscope (Nikon TiE) equipped with a cooled CCD (charge-coupled device) camera (HQ2, Photometrics), with a × 10, 0.3 or 0.5 numerical aperture (NA) objective. We recorded an image every 2 min. When blebbistatin was not used, nuclear position was determined by recording the Hoechst signal with the same temporal resolution. We focused on the passage of the first constriction encountered by the cell. Characteristics of cell passage through constrictions were determined using a custom-made semi-automatized ImageJ macro combined with a Matlab script. The percentage of passage was calculated as the ratio between the number of cells that passed a constriction and the number of cells that encountered a constriction. The cell (or nucleus) passage time represents the time between the cell (nucleus) entering the constriction and the cell (nucleus) back exiting the constriction. The cell non-passage time corresponds to the time spent by the cell in the constriction before changing direction and moving backward.

Cell tracking

LifeAct and Hoechst signals were used to generate the mask of the cell and the nucleus, respectively. A custom-made ImageJ macro combined with a Matlab script was used to analyse cell and nuclear trajectories. Briefly, positions of the cell and nuclear centre of mass and edges (front and back) were detected from the masks at each time point. The change of speed between the channel and the constriction was determined by subtracting, at each time point, the mean cell velocity before the nucleus touched the constriction to the instantaneous speed in the constriction. The normalized velocity variation was then calculated as the ratio between the velocity variation and the mean velocity before the constriction, for each cell.

Quantification of actin enrichment around the nucleus and beads

Hoechst stained DCs from LifeAct-GFP mice or actin-GFP overexpressing HL60-derived neutrophils were loaded in microchannels and imaged using an epifluorescence microscope (Nikon TiE) equipped with a cooled CCD camera (HQ2, Photometrics) and a × 20 dry objective with a high NA (Nikon × 20 0.75 NA DIC or phase contrast). The LifeAct and Hoechst signals were used to generate the mask of the cell and the nucleus, respectively. A custom-made ImageJ macro combined to a Matlab (version R2011a) script was used to analyse the actin dynamics during cell passage through constrictions. The mean actin in constrictions was defined as the ratio between the total LifeAct signal and the cell area in the constriction. This value divided by the mean LifeAct intensity in the cell gave the normalized mean actin intensity inside constrictions. To obtain the normalized mean actin intensity around the nucleus, the mean LifeAct intensity in the mask of the cell cortex around the nucleus was determined and normalized by the mean LifeAct intensity in the whole cell. The mask of the cell cortex was obtained by subtracting a six-times-eroded from a one-time-dilated mask of the cell.

The same method was used to quantify actin dynamics around internalized beads. Beads masks were obtained by hand drawing of beads contours.

Immunofluorescence inside microchannels

To perform immunostaining on cells migrating inside microchannels, the PDMS chamber was reversibly bound to the glass coverslip by plasma activating only the glass coverslip. DCs were allowed to migrate in microchannels for 16 h. Cells were then fixed with 4% paraformaldehyde for 30 min at room temperature. After three washes with PBS, the PDMS was carefully removed to insure cell reachability during immunostaining. Cells were then permeabilized with PBS containing 0.5% Triton for 5 min and rinsed with PBS–5%BSA for 1 h at room temperature. Cells were incubated with primary, then secondary antibodies diluted in PBS–1%BSA. Phalloidin and DAPI staining was performed during the secondary antibody staining. Coverslips were washed with PBS and mounted in custom-made mounting media (Moviol). Image acquisition was performed on an inverted epifluorescence microscope (Nikon TiE) equipped with a cooled CCD camera (HQ2, Photometrics) and a × 100, 1.4 NA oil-immersion objective.

Actin visualization inside collagen gels

Collagen gels were prepared at 3.0 mg ml⁻¹ of Rat-tail collagen (10 mg ml⁻¹, Ibidi). DCs were embedded in polymerizing collagen at a concentration of 2.10⁶ ml⁻¹ and then placed on glass-bottom dishes. After 20 min of collagen gelation at 37 °C, medium was added to equilibrate the gel and cells were fixed with 4% paraformaldehyde for 40 min before being stained with phalloidin.

Actin visualization in ear explants

Ears from C57BL/6 mice were excised and a pair of forceps was used to create a hole.The ventral and dorsal sides of the explant were separated by peeling. The ventral sheet was kept and immunostained with anti-LYVE-1 (R&D Systems) primary antibody, to mark the lymphatic vessels. After washing with media, a secondary antibody against rat (Jackson Immunoresearch, 712-166-150) was used. The ear sheet was then spread flat in a six-well plate and a PDMS block with a central hole of diameter 8 mm was placed on top of each explant. Two hundred thousand LifeAct-GFP-expressing DCs labelled with Hoechst were added in 100 μl of culture medium inside the hole. After 1 h of incubation, the ear sheet was washed with culture medium and then placed with the face on which cells were incubating against the bottom glass slide in a FluoroDish. A block of PDMS was added on top to prevent the explant from moving during imaging. Imaging was performed on an inverted confocal microscope, at 37 °C and with 5% CO₂.

Actin visualization in medaka fish

Imaging was performed on TG(FmpoP::EB3-EGFP/FmpoP::RFP-LifeAct)⁶⁵ transiently expressing the nuclear H2B-CFP marker at 9 days post fertilization, wounded and mounted as previously reported⁶⁶. Time-lapse fluorescence images were acquired with an UltraView VoX spinning disk confocal unit (Perkin Elmer) equipped with an inverted Nikon Eclipse Ti microscope, coupled to a C9100-50 emCCD camera (Hamamatsu) and a Yokogawa CSU-X1 scanning head, and driven by Volocity software (Improvision, Perkin-Elmer). Cyan fluorescent protein/green fluorescent protein (GFP)/red fluorescent protein (RFP) imaging was performed using 405, 488 and 568 nm laser lines with a specific multiband-pass emission filter. Image sequences were acquired at 15–18 s time resolution using, an NA 1.3/ × 40 oil-immersion objective lens and 1 μm step size. Multi-channel images were acquired before switching Z-planes to improve the accuracy of co-localization between channels.

Statistics

GraphPad prism software was used to produce all the graphs. We used the Mann–Whitney test and the Fisher’s test, to determine the significance between two distributions of respectively random and Boolean variables. Mann–Whitney tests were performed using GraphPad prism and Fischer’s tests were performed using using R (version 3.0.2).

Code availability

Codes used to analyse cell passage through constrictions and actin dynamics during cell migration are accessible on request.

Additional Information

How to cite this article: Thiam, H. R. et al. Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments. Nat. Commun. 7:10997 doi: 10.1038/ncomms10997 (2016).

References

Danuser, G., Allard, J. & Mogilner, A. Mathematical modeling of eukaryotic cell migration: insights beyond experiments. Annu. Rev. Cell Dev. Biol. 29, 501–528 (2013) .
Article CAS Google Scholar
Ridley, A. J. et al. Cell migration: integrating signals from front to back. Science 302, 1704–1709 (2003) .
Article CAS ADS Google Scholar
Wilson, C. A. et al. Myosin II contributes to cell-scale actin network treadmilling through network disassembly. Nature 465, 373–377 (2010) .
Article CAS ADS Google Scholar
Chan, C. E. & Odde, D. J. Traction dynamics of filopodia on compliant substrates. Science 322, 1687–1691 (2008) .
Article CAS ADS Google Scholar
Gupton, S. L. & Waterman-Storer, C. M. Spatiotemporal feedback between actomyosin and focal-adhesion systems optimizes rapid cell migration. Cell 125, 1361–1374 (2006) .
Article CAS Google Scholar
Bangasser, B. L., Rosenfeld, S. S. & Odde, D. J. Determinants of maximal force transmission in a motor-clutch model of cell traction in a compliant microenvironment. Biophys. J. 105, 581–592 (2013) .
Article CAS ADS Google Scholar
Lämmermann, T. et al. Rapid leukocyte migration by integrin-independent flowing and squeezing. Nature 453, 51–55 (2008) .
Article ADS Google Scholar
Malawista, S. E. & Chevance, A. d. B. Random locomotion and chemotaxis of human blood polymorphonuclear leukocytes (PMN) in the presence of EDTA: PMN in close quarters require neither leukocyte integrins nor external divalent cations. Proc. Natl Acad. Sci. USA 94, 11577–11582 (1997) .
Article CAS ADS Google Scholar
Tozluoğlu, M. et al. Matrix geometry determines optimal cancer cell migration strategy and modulates response to interventions. Nat. Cell Biol. 15, 751–762 (2013) .
Article Google Scholar
Jacobelli, J. et al. Confinement-optimized three-dimensional T cell amoeboid motility is modulated via myosin IIA-regulated adhesions. Nat. Immunol. 11, 953–961 (2010) .
Article CAS Google Scholar
Lämmermann, T. et al. Cdc42-dependent leading edge coordination is essential for interstitial dendritic cell migration. Blood 113, 5703–5710 (2009) .
Article Google Scholar
Lautenschläger, F. et al. The regulatory role of cell mechanics for migration of differentiating myeloid cells. Proc. Natl Acad. Sci. USA 106, 15696–15701 (2009) .
Article ADS Google Scholar
Wolf, K. et al. Collagen-based cell migration models in vitro and in vivo. Semin. Cell Dev. Biol. 20, 931–941 (2009) .
Article CAS Google Scholar
Janmey, P. A., Euteneuer, U., Traub, P. & Schliwa, M. Viscoelastic properties of vimentin compared with other filamentous biopolymer networks. J. Cell Biol. 113, 155–160 (1991) .
Article CAS Google Scholar
Seltmann, K., Fritsch, A. W., Käs, J. A. & Magin, T. M. Keratins significantly contribute to cell stiffness and impact invasive behavior. Proc. Natl Acad. Sci. USA 110, 18507–18512 (2013) .
Article CAS ADS Google Scholar
Harada, T. et al. Nuclear lamin stiffness is a barrier to 3D migration, but softness can limit survival. J. Cell Biol. 204, 669–682 (2014) .
Article CAS Google Scholar
Wolf, K. et al. Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force. J. Cell Biol. 201, 1069–1084 (2013) .
Article CAS Google Scholar
Krause, M. & Wolf, K. Cancer cell migration in 3D tissue: negotiating space by proteolysis and nuclear deformability. Cell Adhes. Migr 9, 357–366 (2015) .
Article CAS Google Scholar
Pflicke, H. & Sixt, M. Preformed portals facilitate dendritic cell entry into afferent lymphatic vessels. J. Exp. Med. 206, 2925–2935 (2009) .
Article CAS Google Scholar
Rowat, A. C. et al. Nuclear envelope composition determines the ability of Neutrophil-type cells to passage through micron-scale constrictions. J. Biol. Chem. 288, 8610–8618 (2013) .
Article CAS Google Scholar
Davidson, P. M., Denais, C., Bakshi, M. C. & Lammerding, J. Nuclear deformability constitutes a rate-limiting step during cell migration in 3-D environments. Cell. Mol. Bioeng. 7, 293–306 (2014) .
Article CAS Google Scholar
Thomas, D. G. et al. Non-muscle myosin IIB is critical for nuclear translocation during 3D invasion. J. Cell Biol. 210, 583–594 (2015) .
Article CAS Google Scholar
Beadle, C. et al. The role of myosin II in glioma invasion of the brain. Mol. Biol. Cell 19, 3357–3368 (2008) .
Article CAS Google Scholar
Chabaud, M. et al. Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nat. Commun. 6, 7526 (2015) .
Article CAS Google Scholar
Faure-André, G. et al. Regulation of dendritic cell migration by CD74, the MHC class II-associated invariant chain. Science 322, 1705–1710 (2008) .
Article ADS Google Scholar
Wyckoff, J. B., Pinner, S. E., Gschmeissner, S., Condeelis, J. S. & Sahai, E. ROCK- and Myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo. Curr. Biol. 16, 1515–1523 (2006) .
Article CAS Google Scholar
Elliott, H. et al. Myosin II controls cellular branching morphogenesis and migration in three dimensions by minimizing cell-surface curvature. Nat. Cell Biol. 17, 137–147 (2015) .
Article CAS Google Scholar
Heuzé, M. L., Collin, O., Terriac, E., Lennon-Duménil, A.-M. & Piel, M. Cell migration in confinement: a micro-channel-based assay. Methods Mol. Biol. Clifton NJ 769, 415–434 (2011) .
Article Google Scholar
Tanizaki, H. et al. Rho-mDia1 pathway is required for adhesion, migration, and T-cell stimulation in dendritic cells. Blood 116, 5875–5884 (2010) .
Article CAS Google Scholar
Vargas, P. et al. Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells. Nat. Cell Biol. 18, 43–53 (2016) .
Article CAS Google Scholar
Stroka, K. M. et al. Water permeation drives tumor cell migration in confined microenvironments. Cell 157, 611–623 (2014) .
Article CAS Google Scholar
Hetrick, B., Han, M. S., Helgeson, L. A. & Nolen, B. J. Small molecules CK-666 and CK-869 inhibit actin-related protein 2/3 complex by blocking an activating conformational change. Chem. Biol. 20, 701–712 (2013) .
Article CAS Google Scholar
Eichner, A. et al. Lamellipodia and filopodia promote environmental exploration but are dispensable for locomotion of leukocytes. Nat. Cell Biol In Press .
Riedl, J. et al. Lifeact mice for studying F-actin dynamics. Nat. Methods 7, 168–169 (2010) .
Article CAS Google Scholar
Petrie, R. J., Koo, H. & Yamada, K. M. Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix. Science 345, 1062–1065 (2014) .
Article CAS ADS Google Scholar
Zhang, Y. et al. Mouse models of MYH9-related disease: mutations in nonmuscle myosin II-A. Blood 119, 238–250 (2012) .
Article CAS Google Scholar
Le Berre, M., Aubertin, J. & Piel, M. Fine control of nuclear confinement identifies a threshold deformation leading to lamina rupture and induction of specific genes. Integr. Biol. 4, 1406 (2012) .
Article CAS Google Scholar
Swift, J. et al. Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation. Science 341, 1240104 (2013) .
Article Google Scholar
Boukellal, H., Campás, O., Joanny, J. -F., Prost, J. & Sykes, C. Soft Listeria: actin-based propulsion of liquid drops. Phys. Rev. E Stat. Nonlin. Soft Matter Phys. 69, 061906 (2004) .
Article Google Scholar
Kawska, A. et al. How actin network dynamics control the onset of actin-based motility. Proc. Natl Acad. Sci. USA 109, 14440–14445 (2012) .
Article CAS ADS Google Scholar
van Oudenaarden, A. & Theriot, J. A. Cooperative symmetry-breaking by actin polymerization in a model for cell motility. Nat. Cell Biol. 1, 493–499 (1999) .
Article CAS Google Scholar
Risca, V. I. et al. Actin filament curvature biases branching direction. Proc. Natl Acad. Sci. USA 109, 2913–2918 (2012) .
Article CAS ADS Google Scholar
Bieling, P. et al. Force feedback controls motor activity and mechanical properties of self-assembling branched actin networks. Cell 164, 115–127 (2016) .
Article CAS Google Scholar
Tapley, E. C. & Starr, D. A. Connecting the nucleus to the cytoskeleton by SUN–KASH bridges across the nuclear envelope. Curr. Opin. Cell Biol. 25, 57–62 (2013) .
Article CAS Google Scholar
Crisp, M. et al. Coupling of the nucleus and cytoplasm: role of the LINC complex. J. Cell Biol. 172, 41–53 (2006) .
Article CAS Google Scholar
Luxton, G. W. G., Gomes, E. R., Folker, E. S., Worman, H. & Gundersen, G. G. TAN lines. Nucleus 2, 173–181 (2011) .
Article Google Scholar
Stewart, R. M. et al. Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. J. Cell Biol. 209, 403–418 (2015) .
Article CAS Google Scholar
Mori, M. et al. An Arp2/3 nucleated F-actin shell fragments nuclear membranes at nuclear envelope breakdown in starfish oocytes. Curr. Biol. 24, 1421–1428 (2014) .
Article CAS Google Scholar
Khatau, S. B. et al. A perinuclear actin cap regulates nuclear shape. Proc. Natl Acad. Sci. USA 106, 19017–19022 (2009) .
Article CAS ADS Google Scholar
Luxton, G. W. G., Gomes, E. R., Folker, E. S., Vintinner, E. & Gundersen, G. G. Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement. Science 329, 956–959 (2010) .
Article CAS ADS Google Scholar
Gomes, E. R., Jani, S. & Gundersen, G. G. Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell 121, 451–463 (2005) .
Article CAS Google Scholar
Versaevel, M., Grevesse, T. & Gabriele, S. Spatial coordination between cell and nuclear shape within micropatterned endothelial cells. Nat. Commun. 3, 671 (2012) .
Article ADS Google Scholar
Kim, D.-H., Chambliss, A. B. & Wirtz, D. The multi-faceted role of the actin cap in cellular mechanosensation and mechanotransduction. Soft Matter 9, 5516–5523 (2013) .
Article CAS ADS Google Scholar
Gay, O. et al. RefilinB (FAM101B) targets filamin A to organize perinuclear actin networks and regulates nuclear shape. Proc. Natl Acad. Sci. USA 108, 11464–11469 (2011) .
Article CAS ADS Google Scholar
Shao, X., Li, Q., Mogilner, A., Bershadsky, A. D. & Shivashankar, G. V. Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim. Proc. Natl Acad. Sci. USA 112, E2595–E2601 (2015) .
Article CAS ADS Google Scholar
Nigg, E. A. Targets of cyclin-dependent protein kinases. Curr. Opin. Cell Biol. 5, 187–193 (1993) .
Article CAS Google Scholar
Kumar, A. et al. ATR mediates a checkpoint at the nuclear envelope in response to mechanical stress. Cell 158, 633–646 (2014) .
Article CAS Google Scholar
Pajerowski, J. D., Dahl, K. N., Zhong, F. L., Sammak, P. J. & Discher, D. E. Physical plasticity of the nucleus in stem cell differentiation. Proc. Natl Acad. Sci. USA 104, 15619–15624 (2007) .
Article CAS ADS Google Scholar
Parekh, S. H., Chaudhuri, O., Theriot, J. A. & Fletcher, D. A. Loading history determines the velocity of actin-network growth. Nat. Cell Biol. 7, 1219–1223 (2005) .
Article Google Scholar
Caton, M. L., Smith-Raska, M. R. & Reizis, B. Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen. J. Exp. Med. 204, 1653–1664 (2007) .
Article CAS Google Scholar
Srinivas, S. et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 1, 4 (2001) .
Article CAS Google Scholar
Shi, G.-P. et al. Cathepsin S required for normal MHC Class II peptide loading and germinal center development. Immunity 10, 197–206 (1999) .
Article CAS Google Scholar
Peng, J., Wallar, B. J., Flanders, A., Swiatek, P. J. & Alberts, A. S. Disruption of the Diaphanous-related formin Drf1 gene encoding mDia1 reveals a role for Drf3 as an effector for Cdc42. Curr. Biol. 13, 534–545 (2003) .
Article CAS Google Scholar
Millius, A. & Weiner, O. D. Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration. Methods Mol. Biol. Clifton NJ 591, 147–158 (2010) .
Article Google Scholar
Crespo, C. L. et al. The PAR complex controls the spatiotemporal dynamics of F-actin and the MTOC in directionally migrating leukocytes. J. Cell Sci. 127, 4381–4395 (2014) .
Article CAS Google Scholar
Grabher, C. et al. Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka. J. Leukoc. Biol. 81, 263–271 (2007) .
Article CAS Google Scholar

Download references

Acknowledgements

We thank R. Adelstein for providing the Myosin IIA-GFP mice, H. Ploegh for providing I-A^bβ-GFP knock-in mice, Michael Sixt for providing the LifeAct-GFP mice, Dyche Mullins for providing the Lyn-Emerald, Utrophin-mcherry-expressing HL60 cells and Guillaume Charras for providing Actin-GFP expressing HL60 cells. We gratefully acknowledge Nicolas Minc, Clare Waterman, Katarina Wolf, Kristian Franze, Nicolas Manel, Megan King, Anne Houdusse, Dyche Mullins, Lillian Fritz-Laylin, Rachele Elena, Rhoda Hawkins, Michael Sixt and David Nadziejka for critical reading of the manuscript; V. Fraisier, L. Sengmanivong, the Nikon imaging centre and the PICT IBiSA platform at Institut Curie (CNRS UMR144) for technical support in microscopy; the Institut Curie animal facility; Susan Kitchen-Goosen for animal husbandry and technical support; Matthieu Maurin and Paolo Maiuri for their help in image analysis and statistical analysis; Marine Bretou for her help in qPCR; Raphael Voituriez, Anne Bertrand, Gisèle Bone and Mael Le Berre for helpful discussions. A.S.A. has been supported by the Van Andel, Grand Rapids Community and the Michelle Lund Hope Foundations. H.R.T. has been supported by fellowships from the Curie Institute PhD programme and the Ligue Contre le Cancer. This work was funded by Institut Curie and CNRS, as well as grants from the InNaBioSanté foundation (MICEMICO) and from ANR (ANR-09-PIRI-0027), to A.-M.L.-D. and M.P., and an ERC consolidator grant (PROMICO) to M.P.

Author information

Authors and Affiliations

Institut Curie, PSL Research University, CNRS, UMR 144, Paris, F-75005, France
Hawa-Racine Thiam, Pablo Vargas, Nicolas Carpi, Matthew Raab, Emmanuel Terriac & Matthieu Piel
Institut Curie, PSL Research University, INSERM U932, Paris, F-75005, France
Pablo Vargas & Ana-Maria Lennon-Dumenil
Division of Immunology, Transplantation and Infectious Diseases, San Rafaele Scientific Institute, Milan, 20132, Italy
Carolina Lage Crespo
Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, 06520-8002, Connecticut, USA
Megan C. King
National Jewish Health and University of Colorado, Denver, 80206, Colorado, USA
Jordan Jacobelli
Laboratory of Cell Structure and Signal Integration, Van Andel Research Institute, Grand Rapids, 49503, Michigan, USA
Arthur S. Alberts
Helmholtz Centre for Infection Research, Braunschweig, 38124, Inhoffenstrasse 7, Germany
Theresia Stradal

Authors

Hawa-Racine Thiam
View author publications
You can also search for this author in PubMed Google Scholar
Pablo Vargas
View author publications
You can also search for this author in PubMed Google Scholar
Nicolas Carpi
View author publications
You can also search for this author in PubMed Google Scholar
Carolina Lage Crespo
View author publications
You can also search for this author in PubMed Google Scholar
Matthew Raab
View author publications
You can also search for this author in PubMed Google Scholar
Emmanuel Terriac
View author publications
You can also search for this author in PubMed Google Scholar
Megan C. King
View author publications
You can also search for this author in PubMed Google Scholar
Jordan Jacobelli
View author publications
You can also search for this author in PubMed Google Scholar
Arthur S. Alberts
View author publications
You can also search for this author in PubMed Google Scholar
Theresia Stradal
View author publications
You can also search for this author in PubMed Google Scholar
Ana-Maria Lennon-Dumenil
View author publications
You can also search for this author in PubMed Google Scholar
Matthieu Piel
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

H.R.T. designed, carried out and analysed most of the experiments, prepared the manuscript figures and wrote the manuscript. P.V. contributed to DC culture and manipulation, contributed ideas and discussion, and co-developed the assay for immunostaining inside micro-channels. N.C. performed immunostaining in microchannels. C.L.-C. provided the movies on myeloid cell migration in medaka fish. M.R. performed immunostaining in collagen gel (Fig. 3d) as well as the LifeAct-GFP visualization in DCs migrating in mouse ear explant (Supplementary Movie 6). E.T. designed the microchannels used in Fig. 2a and trained H.R.T. in soft photolithography. J.J., A.A. and T.S. respectively provided the CD11c-Cre/MyosinIIA conditional KO bone marrow, the mDia1-KO and the Hem1-KO mice. A.-M.L.-D. provided DC reagents, most mice and co-supervised the work; M.P. designed experiments, supervised the work and wrote the paper.

Corresponding authors

Correspondence to Ana-Maria Lennon-Dumenil or Matthieu Piel.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Figures,

Supplementary Figures 1-15, Supplementary Tables 1-2, Supplementary Discussion and Supplementary References (PDF 3589 kb)

Supplementary Movie 1

Phase contrast time-lapse imaging of DCs migrating through 20 μm long constrictions of various cross-sectional areas (23 μm² = 5 μm x 4.6 μm; 4.5 μm² = 1.5 μm x 3 μm; 2.2 μm² = 1 μm x 2.2 μm). Hoechst staining (green) of DNA allowed nuclear localization. Time in hour: minutes. Each movie is followed by a zoom on a single cell. (AVI 18542 kb)

Supplementary Movie 2

Low resolution (20X binning 2) time-lapse imaging of LifeAct-GFP expressing DCs migrating on a 2D glass coverslip. Cells were treated with DMSO or 50, 100, 250 nM of Latrunculin A. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. (AVI 6942 kb)

Supplementary Movie 3

High resolution (60X, binning 2), time-lapse imaging of a LifeAct-GFP (green) expressing DC migrating through a 15 μm long, 7 μm² (2 μm x 3.5 μm) constrictions. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. (AVI 1661 kb)

Supplementary Movie 4

Low resolution (20X binning 1), time-lapse imaging of LifeAct-GFP (green) expressing DCs migrating through 20 μm long constrictions of various cross-sectional areas (23 μm² = 5 μm x 4.8 μm; 12 μm² = 3 μm x 4 μm. 4.5 μm² = 1.5 μm x 3 μm; 2.2 μm² = 1 μm x 2.2 μm). Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. Each movie is followed by a zoom on a single cell. (AVI 15206 kb)

Supplementary Movie 5

Time-lapse imaging of LifeAct-GFP (green) expressing dendritic cell migrating through 15 μm long, 7 μm² (2 μm x 3.5 μm) constrictions. Hoechst staining (red) of DNA allowed nuclear localization. Channels are coated with PLL-PEG to prevent the formation of adhesion complexes. Time in hour: minutes. Movies of single channels are concatenated for better visibility. (AVI 6140 kb)

Supplementary Movie 6

Time-lape imaging of LifeAct-GFP (green) expressing DCs migrating in a mouse ear explant. Hoechst staining (red) of DNA allowed nuclear localization. Lymph vessels were stained with a rat-anti-Lyve-1 antibody (blue). Time in minutes: seconds. The movie is followed by a zoom on a single cell then by a rotated view of this cell. (AVI 7831 kb)

Supplementary Movie 7

Time-lapse imaging of RFP-LifeAct (actin, green) and H2B-CFP (chromatin, red) expressing myeloid cells migrating in mekada fish after wounding of the tail. The movie shows two representative cells. Time in hour: minutes: seconds. White arrows indicate the presence of an actin accumulation. (AVI 4944 kb)

Supplementary Movie 8

Time-lapse imaging of an invariant chain knock-out dendritic cell migrating through a 15 μm long, 7 μm² (2 μm x 3.5 μm) constriction. Hoechst staining (red) of DNA allowed nuclear localization. Reflection interference contrast microscopy was used to visualize cell-substrate contact areas. Time in hour: minutes. (AVI 1039 kb)

Supplementary Movie 9

Low resolution (20X binning 2) time-lapse imaging of LifeAct-GFP expressing DCs migrating through 15 μm long, 7 μm² (2 μm x 3.5 μm) constrictions. Cells were treated with DMSO or 50nM of Latrunculin A. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. (AVI 1145 kb)

Supplementary Movie 10

Time-lapse imaging of LifeAct-GFP (green) expressing DCs migrating through 15 μm long, 7 μm² (2 μm x 3.5 μm) constrictions. Cells were treated with DMSO or with 50 μM CK666. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. The first movie is followed by a zoom on a single passing cell. The second movie is followed by a zoom on a single non-passing cell, then by a zoom on a passing cell. (AVI 2632 kb)

Supplementary Movie 11

Myosin IIA-GFP (green) expressing DCs migrating through 15 μm long, 7 μm² (2 μm x 3.5 μm) constrictions. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. The movie is followed by a zoom on a single cell. (AVI 4149 kb)

Supplementary Movie 12

Low resolution (20X binning 1), time-lapse imaging of LifeAct-GFP (green) expressing DCs migrating through 20 μm long constrictions of various cross-sectional areas (10 μm² = 2.5 μm x 4 μm; 12.6 μm² = 3 μm x 4.2 μm; 14.7 μm² = 3.5 μm x 4.2 μm). Bright spots in phase contrast represent the internalized beads. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. (AVI 7794 kb)

Supplementary Movie 13

Low resolution (20X binning 2), time-lapse imaging of LifeAct-GFP (green) expressing DCs migrating through 3 μm wide and 20 μm long constrictions. DCs have internalized beads of 3 μm and 2 μm of diameter. Bright spots in phase contrast represent the internalized 3 μm beads while 2 μm beads are in cyan. Hoechst staining (red) of DNA allowed nuclear localization. Time in hour: minutes. White arrows indicate the position of 2 μm beads. Time in hour: minutes. (AVI 5572 kb)

Rights and permissions

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Reprints and permissions

About this article

Cite this article

Thiam, HR., Vargas, P., Carpi, N. et al. Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments. Nat Commun 7, 10997 (2016). https://doi.org/10.1038/ncomms10997

Download citation

Received: 08 December 2015
Accepted: 09 February 2016
Published: 15 March 2016
DOI: https://doi.org/10.1038/ncomms10997

This article is cited by

ANTXR1 deficiency promotes fibroblast senescence: implications for GAPO syndrome as a progeroid disorder
- Matthias Przyklenk
- Shreya Karmacharya
- Alvise Schiavinato
Scientific Reports (2024)
LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion
- Yaiza Jung-Garcia
- Oscar Maiques
- Jeremy G. Carlton
Nature Cell Biology (2023)
Compressive forces stabilize microtubules in living cells
- Yuhui Li
- Ondřej Kučera
- Manuel Théry
Nature Materials (2023)
Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties
- Antoine Leblanc-Hotte
- Cindy Audiger
- Sylvie Lesage
Scientific Reports (2023)
Mena regulates nesprin-2 to control actin–nuclear lamina associations, trans-nuclear membrane signalling and gene expression
- Frederic Li Mow Chee
- Bruno Beernaert
- Adam Byron
Nature Communications (2023)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.