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MiR-101 regulates apoptosis of trophoblast HTR-8/SVneo cells by targeting endoplasmic reticulum (ER) protein 44 during preeclampsia

Journal of Human Hypertension volume 28, pages 610–616 (2014)Cite this article

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Abstract

To investigate a possible association between miR-101 and apoptosis of human trophoblast cells mediated by endoplasmic reticulum protein 44 (ERp44) in preeclampsia (PE), we explored the expression of miR-101 in PE placentas (n=30) compared with normotensive pregnant placentas (n=30) and the correlation between miR-101 and ERp44 was also analyzed. Furthermore, both the apoptotic rate of trophoblast cells and the ER stress-induced apoptotic proteins were assayed when the HTR-8/SVneo cells were treated with miR-101 mimics or inhibitors in vitro. We found a lower expression of miR-101 and an inverse correlation between miR-101 and ERp44 protein in PE placentas. Upregulation of miR-101 expression could inhibit trophoblast HTR-8/SVneo cell apoptosis and repress ER stress-induced apoptotic proteins by targeting ERp44 in vitro, whereas inhibition of miR-101 could induce HTR-8/SVneo cell apoptosis. Our findings indicated that overexpression of miR-101 could downregulate ERp44 and suppress apoptosis in trophoblast cells during PE. Therefore, loss of miR-101 expression could contribute to ER stress-induced trophoblast cell apoptosis by targeting ERp44.

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Acknowledgements

This study was supported by The National Natural Science Foundation of China, Grant Nos 81070511 and 81270710.

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  1. Y Zou, Z Jiang and X Yu: These authors contributed equally to this work and should be regarded as joint first authors.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China

    Y Zou, Z Jiang, Y Zhang, W Wang, Z Ge & L Sun

  2. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China

    X Yu, M Sun & W De

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Correspondence to L Sun.

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Zou, Y., Jiang, Z., Yu, X. et al. MiR-101 regulates apoptosis of trophoblast HTR-8/SVneo cells by targeting endoplasmic reticulum (ER) protein 44 during preeclampsia. J Hum Hypertens 28, 610–616 (2014). https://doi.org/10.1038/jhh.2014.35

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