Abstract
Objective:
To investigate whether prohibitin (PHB) is a target gene in adipocyte differentiation and modulates insulin-induced adipocyte differentiation.
Design:
3T3-L1 preadipocyte overexpressing wild-type PHB and PHB mutant (lacking tyrosine-114 phosphorylation site) with or without insulin.
Results:
The treatment of 3T3-L1 fibroblasts with insulin or peroxisome proliferator-activated receptor-γ agonist resulted in an upregulation of PHB in a dose- and time-dependent manner. An analysis of the PHB promoter sequence revealed the presence of putative insulin-response elements and CCAAT/enhancer-binding protein transcription elements within ∼1 kb upstream of the translation initiation site. The functional relevance of these sites was determined using reporter gene assay. Surprisingly, PHB was also found to be regulated by leptin. Furthermore, the overexpression of PHB in 3T3-L1 fibroblasts was sufficient to induce adipogenesis.
Conclusion:
In summary, we have identified PHB as an important protein in adipocyte differentiation.
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Acknowledgements
We thank Dr Arianne Theiss (University of Emory) and Dr Tooru Mizuno (University of Manitoba) for providing PHB-luciferase constructs and mice tissue samples, respectively. This work was supported by funds from the Natural Sciences and Engineering Research Council of Canada, Canada Foundation for Innovation and Manitoba Health Research Council (MHRC). SM is a recipient of the MMSF Career Development Award and SRA is supported by an MHRC Postdoctoral Fellowship.
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Ande, S., Xu, Z., Gu, Y. et al. Prohibitin has an important role in adipocyte differentiation. Int J Obes 36, 1236–1244 (2012). https://doi.org/10.1038/ijo.2011.227
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DOI: https://doi.org/10.1038/ijo.2011.227
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