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The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue

International Journal of Obesity volume 36, pages 1360–1365 (2012)Cite this article

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Abstract

Objective:

Hypoadiponectinemia observed in obesity is associated with insulin resistance, diabetes and atherosclerosis. The aim of the present study was to investigate secretion of adiponectin and its multimeric isoforms by explants derived from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in obese and non-obese subjects.

Design:

Paired samples of SAT and VAT and blood samples were obtained from 23 subjects (10 non-obese and 13 obese) undergoing elective abdominal surgery. Total adiponectin quantities and adiponectin isoforms were measured in conditioned media of explants derived from SAT and VAT using enzyme-linked immunosorbent assay and non-denaturing western blot, respectively.

Results:

Total adiponectin plasma levels were lower in obese than in non-obese subjects (P<0.05). Secretion of total adiponectin in adipose tissue (AT) explants was lower in obese than in non-obese subjects in SAT (P<0.05) but not in VAT. In both, SAT and VAT, the most abundant isoform released into conditioned media was the high-molecular weight (HMW) form. Its relative proportion in relation to total adiponectin was higher in conditioned media of explants from both fat depots when compared with plasma (P<0.001). The proportion of secreted HMW vs total adiponectin was higher in VAT than in SAT explants in the group of non-obese individuals (49.3±3.1% in VAT vs 40.6±2.8% in SAT; P<0.01), whereas no difference between the two depots was found in obese subjects (46.2±3.0 % in VAT vs 46.0±2.4 % in SAT).

Conclusion:

Obesity is associated with the decrease of total adiponectin secretion in SAT. The profile of adiponectin isoforms secreted by SAT and VAT explants differs from that in plasma. Secretion of total adiponectin and HMW isoform of adiponectin are different in obese and non-obese subjects in relation to AT depot.

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Acknowledgements

The study was supported by GAUK1269/08/C/2008; Integrated Project HEPADIP (http://www.hepadip.org), Contract No. LSH-2003-1.1.3-1, and Collaborative Project ADAPT (http://www.adapt-eu.net), Contract No. HEALTH-F2-2008-2011 00). The Research Project of the Czech Ministry of Education MSM 0021620814. We would like to thank to Karin Doležalová, MD, Anna Jungwirthová, MD and David Melechovskỳ, MD, for the collection of adipose tissue samples and cooperation on the study.

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Authors and Affiliations

  1. Department of Sport Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic

    Z Kovacova, M Tencerova, Z Wedellova, L Rossmeislova, J Polak & V Stich

  2. Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Charles University, Prague, Czech Republic

    Z Kovacova, M Tencerova, Z Wedellova, L Rossmeislova & V Stich

  3. Franco-Czech Laboratory for Clinical Research on Obesity, Inserm, U1048, Toulouse, France

    B Roussel & D Langin

  4. Laboratoire de recherches sur les Obésités, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), UMR 1048 Inserm, Université Paul Sabatier; Laboratoire de biochimie, Institut Fédératif de Biologie, CHU Toulouse,

    B Roussel & D Langin

  5. Laboratoire de biochimie, Institut Fédératif de Biologie, CHU Toulouse, Toulouse, France

    B Roussel & D Langin

  6. 2nd Internal Medicine Department, University Hospital of Kralovske Vinohrady, Prague, Czech Republic

    Z Wedellova & J Polak

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Kovacova, Z., Tencerova, M., Roussel, B. et al. The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue. Int J Obes 36, 1360–1365 (2012). https://doi.org/10.1038/ijo.2011.223

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