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Efficient delivery of lentiviral vectors into resting human CD4 T cells

Gene Therapy volume 21, pages 444–449 (2014)Cite this article

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A Corrigendum to this article was published on 03 March 2016

Abstract

Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.

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Acknowledgements

We thank Dr Matthew Spindler in the laboratory of Dr Bruce Conklin for the generous gift of pSico-mCherry HIV LV vector. We thank Dr Marielle Cavrois, Marianne Gesner and Jaime Tawney for assistance with flow cytometry. We also thank Dr Gary Howard, Crystal Herron and Dr Anna Lisa Lucido for editorial assistance, John C.W. Carroll, Giovanni Maki and Teresa Roberts for graphics arts, and Robin Givens and Sue Cammack for administrative assistance. Special thanks to Jason Neidleman for stimulating discussions and technical advice. Funding for this project was provided by the UCSF/Robert John Sabo Trust Award (GD), NIH/NIAID R21 AI102782 (WCG), 1DP11036502 (WCG) and NIH/NIAID U19 AI096113 (WCG). We thank the UCSF-GIVI CFAR for providing core services that facilitated these studies (CFAR P30 AI027763).

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Author notes

  1. X Geng and G Doitsh: These authors contributed equally to this work.

Authors and Affiliations

  1. Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA

    X Geng, G Doitsh, Z Yang, N L K Galloway & W C Greene

  2. Department of Medicine, University of California, San Francisco, San Francisco, CA, USA

    W C Greene

  3. Department of Microbiology and Immunology, University of California, San Francisco, CA, USA

    W C Greene

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Correspondence to W C Greene.

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Geng, X., Doitsh, G., Yang, Z. et al. Efficient delivery of lentiviral vectors into resting human CD4 T cells. Gene Ther 21, 444–449 (2014). https://doi.org/10.1038/gt.2014.5

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