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Vitamins and plant ingredients

Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants

European Journal of Clinical Nutrition volume 66, pages 1054–1058 (2012)Cite this article

Subjects

Abstract

BACKGROUND/OBJECTIVES:

Oxidative stress (OS) is thought to be involved in both development of type 1 diabetes (T1D) and its further complications. In this study, certain biomarkers of OS were compared among the subjects with T1D, their non-diabetic siblings and unrelated healthy controls.

SUBJECTS/METHODS:

Known cases of T1D from both sexes aged 5–25 years were enrolled in a case–control study (n1=60). There were two control groups; non-diabetic siblings (n2=60) and unrelated apparently healthy subjects (n3=60). Anthropometric, dietary and laboratory assessments were done.

RESULTS:

There was no significant difference in dietary data among the groups. Total antioxidant capacity was significantly lower in T1D than both related and unrelated controls (1.6±0.05, 1.7±0.05 and 1.8±0.06 mmol BSA equivalent/l, respectively, P=0.044). Both T1D subjects and their siblings showed lower glutathione peroxidase (GSH-px) levels (median (interquartile range): 22.2 (28.6), 29.9 (23) and 41.8 (73.6) U/ml, respectively, P=0.006). On the contrary, superoxide dismutase concentrations were significantly higher in T1D group and the siblings than unrelated healthy controls (243 (45.3), 157.8 (176.9) and 27.9 (8.7) U/l, respectively, P<0.001). Serum concentrations of GSH correlated with energy intake in the siblings (r=0.521, P<0.001) and unrelated controls (r=0.268, P=0.042) but not in T1D group. The associations remained significant after controlling for blood glucose (r=0.437, P=0.001 and r=0.420, P=0.011, respectively) in both the groups.

CONCLUSION:

Augmented OS in the siblings may indicate an increased requirement for antioxidants in genetically diabetes-prone subjects.

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Acknowledgements

This study was funded by the National Nutrition and Food Technology Research Institute. All laboratory bench works were performed at the Laboratory of Nutrition Research, National Nutrition and Food Technology Research Institute. Finally, we do appreciate all the subjects who devotedly participated in this study.

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Authors and Affiliations

  1. Laboratory of Nutrition Research, National Nutrition and Food Technology Research Institute (NNFTRI), Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    T R Neyestani, A Kalayi, N Shariatzadeh & A Houshiarrad

  2. Department of Nutrition and Biochemistry, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Z Ghandchi & M-R Eshraghian

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  1. T R Neyestani
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  2. Z Ghandchi
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Correspondence to T R Neyestani.

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Contributors: Dr TR Neyestani designed and supervised the study, involved in laboratory analyses and wrote the manuscript. Ms Z Ghandchi performed most parts of laboratory analyses, involved in the whole-field works. All statistical analyses were done under Dr MR Eshraghian's supervision. Mrs N Shariatzadeh and Mr A Kalayi had a sincere cooperation in all the laboratory investigations. Finally, dietary assessments were performed under Ms A Houshiarrad guidance.

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Neyestani, T., Ghandchi, Z., Eshraghian, MR. et al. Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants. Eur J Clin Nutr 66, 1054–1058 (2012). https://doi.org/10.1038/ejcn.2012.81

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