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Molecular characterization of an apoptotic strain of Newcastle disease virus isolated from an outbreak in India

Cancer Gene Therapy volume 22, pages 402–409 (2015)Cite this article

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Abstract

Newcastle disease (ND) is a highly contagious disease of poultry. The ND virus (NDV) encodes an error-prone RNA-dependent RNA polymerase which can cause high mutation rate leading to the emergence of its new antigenic variants. Antigenic difference of NDV strains may result in massive outbreak in vaccinated and unvaccinated poultry flocks around the globe. Apart from its pathogenic potential NDV has been explored as an oncolytic agent for a broad range of human cancers. In the present study, we isolated a novel NDV strain from an outbreak in chicken flock from the eastern part of India. Molecular characterization showed the NDV strain to be virulent in nature. The complete genome sequence analysis of the newly isolated strains belongs to genotype XIII. Moreover, the newly isolated strain of NDV showed positive results in various apoptotic assays in human breast cancer cells, MCF-7. The study will be useful to explore the possibility of using a newly isolated strain of NDV for virotherapy.

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Acknowledgements

We thank Drs Sobhakant pathak and Shivanand Kanshi, IAHP, Kanke Ranchi for providing NDV outbreak samples. We thank Sudhir Morla and all our laboratory members for their excellent technical assistance and help. We are thankful to Dr Monika Koul for proof reading the manuscript. The NDV research in our laboratory is currently supported by the department of biotechnology (NER-BPMC/2013/134/AAB21), the department of science and technology (IFA-LSBM-34) and the board of research on nuclear sciences (2012/20/37B/06/BRNS).

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  1. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India

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Correspondence to S Kumar.

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Kumar, U., Kumar, S. Molecular characterization of an apoptotic strain of Newcastle disease virus isolated from an outbreak in India. Cancer Gene Ther 22, 402–409 (2015). https://doi.org/10.1038/cgt.2015.35

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