Establishment of cell–cell adhesion is crucial in embryonic development as well as within the stem cell niches of an adult. Adhesion between macrophages and erythroblasts is required for the formation of erythroblastic islands, specialized niches where erythroblasts proliferate and differentiate to produce red blood cells throughout life. The Eph family is the largest known family of receptor tyrosine kinases (RTKs) and controls cell adhesion, migration, invasion and morphology by modulating integrin and adhesion molecule activity and by modifying the actin cytoskeleton. Here, we identify the proteoglycan agrin as a novel regulator of Eph receptor signaling and characterize a novel mechanism controlling cell–cell adhesion and red cell development within the erythroid niche. We demonstrate that agrin induces clustering and activation of EphB1 receptors on developing erythroblasts, leading to the activation of α5β1 integrins. In agreement, agrin knockout mice display severe anemia owing to defective adhesion to macrophages and impaired maturation of erythroid cells. These results position agrin-EphB1 as a novel key signaling couple regulating cell adhesion and erythropoiesis.
During embryonic development, adhesive interactions trigger a variety of morphogenetic processes throughout the organism, involving either direct cell–cell contact or adhesion to extracellular matrix networks. Eph receptors form the largest subfamily of receptor tyrosine kinases (RTKs) and, together with their ligands ephrins, control cell adhesion, migration, invasion and morphology by influencing integrin and intercellular adhesion molecule activity and by modification of actin cytoskeleton organization.1, 2 Eph/ephrin molecules are involved in a wide array of developmental processes such as cardiovascular and skeletal development, axon guidance and tissue patterning.3 Although Eph/ephrins have been studied classically in a developmental context, their physiological functions in the adult came to light, as they have been implicated in bone homeostasis,4 in red blood cell differentiation,5 and in alterations that lead to congenital diseases and cancer.6
Erythropoiesis, the process by which red blood cells are produced, is pivotal during all stages of life to all the vertebrates’ healthy existence. In adults, red blood cells are the terminally differentiated, end-product cells of a complex hierarchy of hematopoietic progenitors that become progressively restricted to the erythroid lineage.7 Mouse erythropoiesis takes place in the fetal liver between embryonic days 12.5 and 15.5, in the neonatal spleen, and in both spleen and bone marrow of adults. Definitive erythropoiesis takes place in specialized niches, the erythoblastic islands (EI),8 consisting of a central macrophage that extends cytoplasmic protrusions to a ring of surrounding erythroid precursors in different stages of maturation. It has been proposed that EI allow macrophages to provide nutrients and survival signals to the erythroblasts, to promote enucleation and to phagocytize the extruded erythroblast nuclei.9, 10 Recent advances in the understanding of the interactions within the erythroid niches have pointed out the extraordinary complexity of the adhesive interaction system that involves a wide range of ligands, such as β1 integrin, CD44, Lu and ICAM-4.11, 12, 13
Agrin is an extracellular matrix protein belonging to the heterogeneous family of heparan sulfate proteoglycans. Its best-characterized role is in the development of the neuromuscular junction during embryogenesis where it is expressed by motor neurons and leads to clustering and activation of the RTK MuSK.14 However, agrin is expressed in many tissues and its non-neuronal functions are poorly understood. We previously demonstrated that in the hematopoietic compartment agrin signals through the dystroglycan (DG) receptor by activating the Grb2-SOS-Ras signaling pathway15, 16 required for survival and differentiation of hematopoietic stem cells.
In the present work, we have addressed the role of agrin in erythropoiesis. We provide evidence for a novel mechanism, mediated by the agrin-DG signaling pathway, regulating the fitness of the erythroid niche.
Agrin knockout mice display an anemic phenotype
Mice lacking a functional agrin gene display embryonic lethality because of neuromuscular synapse dysfunctions, thus strongly limiting the studies in agrin-deficient adult animals.17 To overcome this limitation, in our previous15, 16 and present studies, we took advantage of the Musk-L;Agrn−/− mouse model, null for all agrin isoforms but carrying a transgene that increases Musk expression in skeletal muscle.18 Musk-L;Agrn−/− mice live longer, but still die postnatally between day (P) 7 and P10 for unknown reasons18 and our experiments were therefore performed with P5 mice. Musk-L;Agrn+/+ or Musk-L;Agrn+/− littermates (referred to as control mice) did not differ in any of the analyzed phenotypes,15 and hence, were not further distinguished. As already described,15 P5 Musk-L;Agrn−/− mice (referred to hereafter as agrin-deficient mice) are characterized by smaller size than control littermates, with a reduced number of hematopoietic cells in bone marrow, spleen and blood. In addition, agrin-deficient mice showed major alterations in the spleen, which is an important site of extramedullary hematopoiesis in neonatal mice and contains a large percentage of proliferating cells, among which about 50% are erythroblasts. Agrin-deficient mice showed a significant reduction in the spleen-to-body weight ratio and in the total number of splenocytes (Figure 1a). The immunohistochemical analysis of the erythroid-specific glycophorin-A-associated Ter-119 marker on spleen sections showed a significant reduction of the red pulp in agrin-deficient mice as compared with their control littermates (Figure 1b). In agreement, blood analyses indicated that hematocrit, hemoglobin, red blood cell and reticulocyte count indices were strongly reduced in agrin-deficient mice (Figure 1c). These results indicate that erythropoiesis in agrin-deficient mice is severely impaired.
Cell-autonomous control of late erythropoiesis by agrin
In agreement with our previous study showing bone marrow hypoplasia, as well as a reduction of the Lin−IL7R−c-Kit+Sca-1+ (LSK) HSC population,15 the analysis of bone marrow progenitors showed that both frequency and absolute number of megakaryocyte erythroid progenitors (MEP) were reduced in agrin-deficient (Musk-L;Agrn−/−) mice as compared with control littermates (Supplementary Figure 1A). This drop was paralleled by increased apoptosis in agrin-deficient MEP (Supplementary Figure 1B), suggesting a role for agrin and/or its receptor in promoting MEP survival. Indeed, we found that MEP progenitors express both agrin and its hematopoietic receptor α-DG,15, 16 as detected by flow cytometry analysis (Supplementary Figure 1C).
We then examined whether agrin deficiency affected the different stages of the erythroid maturation process. On the basis of the dynamic changes in membrane protein expression that characterize each erythroid differentiation step, we performed flow cytometry analysis with the erythroid-specific Ter-119 and the non-erythroid-specific transferrin receptor CD71 markers13, 19, 20 that, together, identify four subpopulations (R1-R4) representing sequential stages of maturation: proerythroblasts and early basophilic erythroblasts (R1); early and late basophilic erythroblasts (R2); chromatophilic and orthochromatophilic erythroblasts (R3); and late orthochromatophilic erythroblasts and reticulocytes (R4) (Figure 2a). Similar results were obtained using the double staining CD44/TER-11921 (not shown). Confocal microscopy and flow cytometry were used to validate the expression of agrin and its ligand α-DG15, 16 in control R1-R4 subsets; the results indicate that both the molecules are expressed in R1-R2 subsets (Figure 2a); real-time PCR analysis confirmed that agrin is endogenously produced by R1-R3 erythroid cell subsets (Supplementary Figure 2). Then, we analyzed the R1-R4 erythroid populations in agrin-deficient mice (Figure 2b). Interestingly, as compared with their littermate controls, agrin-deficient mice exhibited a reduction in the cell number of all R1-R4 erythroid cells in the spleen, although the defect was particularly dramatic in the case of the R2 subset (100.00±24,92 ctrl; 6,38±3.11 Musk-L;Agrn−/−) (Figure 2b). The reduction in cell number was accompanied by a significant increase in ex vivo apoptosis observed in the erythroid cells of agrin-deficient mice as compared with control ones (Figure 2c), whereas CD44 expression and cell size (expressed as FSC MFI) were similar in the two groups (Supplementary Figures 3A and 3B), suggesting that a reduced cell viability was responsible for the observed decline of the erythroid populations.
Agrin-deficient mice might have multiple defects that could indirectly cause the observed impairment of erythroid cell development.16 Moreover, the analysis of cell populations in agrin-deficient mice does not allow distinguishing between a cell-autonomous or niche-derived mechanism responsible for the defective erythropoiesis in the absence of agrin. This hypothesis can only be tested performing BM transfer experiments. Lethally irradiated wild-type recipients (CD45.1) were reconstituted using total bone marrow cells from either agrin-deficient or control mice (CD45.2), and the frequency of erythroid subsets was analyzed after 9 weeks. The frequency of peripheral blood hematopoietic cells expressing CD45.2 was quantified by flow cytometry, demonstrating comparable reconstitution in all recipients, that is, >90% for mice that received either control or Musk-L;Agrn−/− bone marrow cells (not shown and Mazzon et al.15). In an agrin-sufficient stromal niche,15 the generation of agrin-deficient MEP progenitors was not impaired and, interestingly, an increase in MEP numbers was detected in wild-type recipients reconstituted with agrin-deficient bone marrow cells (Figure 2d). We thus assessed the in vitro culture growth of P5 erythroid progenitors in burst-forming unit erythroid colony assay. In agreement with the in vivo analysis, the results showed no significant differences between control and agrin-deficient MEP (data not shown).
Whereas the experiments described above (Figure 2d and Supplementary Figure 4) demonstrated a niche-dependent role of agrin in MEP homeostasis, the analysis of agrin-deficient erythroid subsets performed in the BMT recipients showed a persistent defect in the cell number as compared with the control counterpart (Figure 2e). These results support a cell-autonomous role of agrin in the control of late erythroid maturation but not of erythroid precursors.
Agrin regulates adhesive interactions at the erythroblastic islands
The observed reduced viability of agrin-deficient erythroid cells might be explained by a defective formation of EI in the absence of agrin. Native EI, isolated ex vivo from spleens of agrin-deficient or control mice (Figure 3a), as well as islands reconstituted in vitro from bone marrow cells (Figure 3b), were analyzed by confocal microscopy using the erythroid-specific TER-119 and the macrophage-specific F4/80 markers. Agrin-deficient islands exhibited a very low number of erythroid cells per macrophage, suggesting an important role of this molecule in the formation of the niche. Moreover, co-cultures of R2 cells isolated from either control or Musk-L;Agrn−/− mice with control F4/80+ macrophages demonstrated that agrin deficiency affects red cell viability, as shown by the significantly higher number of annexin-V+/Ter119+ cells among agrin-deficient erythroid cells (Supplementary Figure 4). Agrin is also expressed by macrophages16 and the dysfunctional state of agrin-deficient macrophages has already been demonstrated.16 Thus, we analyzed EI reconstituted using either agrin-deficient macrophages and control erythroblasts or control macrophages and agrin-deficient erythroblasts (Figure 3c). The results indicated that, in order to establish proper adhesive interactions that are required to sustain erythroblast viability, both cell types must express agrin.
We have previously demonstrated that in the hematopoietic compartment agrin signals through the DG complex.15, 16 Thus, we speculated that inhibition of agrin-DG interactions would result in defective EI formation. Using an antibody that blocks the interaction of agrin with the α−DG receptor,15, 16 we inhibited EI formation in cells from control mice (Figure 3d), confirming the data obtained with agrin-deficient cells. Again, the pre-incubation of either macrophages or erythroblasts with the blocking antibody resulted in impaired formation of EI, indicating that both cell types require agrin and α-DG receptor for optimal cell–cell adhesion at the erythroid niche (Figure 3e). However, the results also indicate that, for the stability of the EI, the agrin-DG signaling is more critical for erythroid cells than macrophages.
Agrin signaling through DG results in EphB1 RTK activation
At the neuromuscular junction, agrin binds to Lipoprotein-related receptor 4 (Lrp4) and thus mediates triggering of the RTK MuSK.18, 22 We therefore asked whether the interaction of agrin with DG at the EI results in triggering of a member of the RTK family. R2 erythroid cells, unstimulated or stimulated for 5 and 30 min with agrin, were analyzed using a phospho-RTK array, which simultaneously determines relative phosphorylation levels in over 39 different kinases. Analysis of the subsequent phosphorylation profiles revealed that agrin stimulation induces the activation of EphB1 tyrosine kinase that depends on agrin-DG interaction (Figure 4a). This result was confirmed by western blot analysis of R2 erythroid cell lysates with a specific antibody against the phosphorylated form of EphB1 (Supplementary Figure 5). Eph receptor activation and signaling relies on clustering.6 Tyrosine phosphorylation of Eph receptors requires presentation of ephrin ligands in either clustered or membrane-attached forms,23 and specific Eph responses occur only at higher ligand oligomerization states.24 However, some studies suggested that Eph receptor activation is not completely explained by the model described above and indicated that ligand binding may not be required for receptor clustering and phosphorylation.25, 26 We therefore asked whether agrin induced EphB1 clustering in erythroblasts. To this end, R2 cells isolated from control mice were stimulated with agrin for 10 min, stained with anti-EphB1 antibodies and analyzed by confocal microscopy. Agrin stimulation induced EphB1 receptor capping (77.9±12.7% of R2 primary cells showing EphB1 receptor clustering, Figure 4b), which was completely abrogated by pre-incubation of R2 cells with the agrin-DG blocking antibody (77.9±12.7% of R2 primary cells showing EphB1 receptor clustering versus 13.8±1.6%, Figure 4b). In agreement, agrin stimulation induced tyrosine phosphorylation of β-DG in the K562 human erythromyeloblastoid leukemia cell line (Supplementary Figure 6), as already described in neurons,27 as well as DG-mediated actin polymerization in R2 primary cells (Figure 4c) and co-capping of the phosphorylated β-DG with EphB1 at the R2 cell surface (Pearson's coefficient=0.76±0.1, P=0.0003; Figure 4d). The physical interaction between DG and EphB1 was confirmed by co-immunoprecipitation experiments in the K562 cell line (Figure 4e).
The EphB1 downstream response is known to activate αvβ3 and α5β1 integrins and thus promote cell adhesion.28 We therefore examined whether agrin-induced triggering of EphB1 might result in the activation of αvβ3 and α5β1 integrins. Agrin stimulation of R2 erythroblasts induced the expression of both α5 and β1 integrins on the cell surface, and phosphorylation of β1 (Figure 4f). In contrast, no statistically significant difference was observed for αv and β3 integrins (Supplementary Figure 7).
Erythrocytes, produced through a process named erythropoiesis, are pivotal for the existence of all vertebrates and, indeed, agrin-deficient mice displayed overt and severe signs of anemia, with significant reductions in hematocrit, hemoglobin content and circulating red blood cells. However, no defects in fetal erythropoiesis (E15 and E17) were detected (not shown), indicating that agrin is specifically required for the regulation of postnatal erythroid development. These data are consistent with our previous report15 showing that the hematopoietic stem cell dysfunctions caused by agrin deficiency are restricted to postnatal life. The reason why agrin is specifically required for adult hematopoiesis and erythropoiesis is not known, but we speculate that other molecules may substitute agrin in the fetal liver microenvironment. The expression of both agrin and its receptor α-DG in red cells seems to be developmentally regulated and this is consistent with the modulation of expression of adhesion molecules observed during maturation of erythroid cells.21 The reconstitution experiments performed in this study demonstrate that, even in a permissive niche, agrin-deficient hematopoietic cells display defective erythroid maturation, thus highlighting an important and novel cell-autonomous role of agrin in erythropoiesis. Consistently, both agrin and its receptor α-DG are expressed on erythroid subsets during erythropoiesis. Although α-DG expression appears similar at all maturation stages analyzed, in the case of agrin, changes in mRNA and membrane-bound protein do not correlate. This may depend on more than one reason: (i) in R1 cells the membrane-bound agrin may derive from the extracellular microenvironment; (ii) mRNA processing and stability as well as protein stability and modifications may be responsible for the different results observed by analyzing mRNA and protein expression; (iii) finally, we cannot formally exclude the presence in erythroid cells of agrin isoforms not detected by our primers. The fluctuations in membrane-bound agrin expression during the erythropoiesis process are consistent with a previous study21 showing modulation of adhesion molecule expression during the maturation of erythroid cells.
Growing evidence supports the concept of EI as microenvironmental niches where cell–cell adhesive interactions, in concert with cytokines, are crucial for terminal erythroid differentiation and regulation of apoptosis.8, 29 Recent advances in the understanding of the cellular and molecular interactions within the erythroid niches have pointed out the extraordinary complexity of the adhesive interaction system, where both homophilic interactions, such as those mediated by the erythroblast macrophage protein (Emp),30 as well as heterophilic ones, involving, for example, α4β1 integrin and VCAM-1,31 are critical for island integrity13 although the precise roles of these adhesive connections are still unclear.9 Here, we demonstrate for the first time that agrin induces EphB1 phosphorylation in the primary R2 erythroid subset through its interaction with the α-DG receptor. We show that EphB1 and DG are physically and functionally associated, and provide evidence for a key role of agrin in the subsequent α5β1 integrin activation, thus mediating the stability of the interaction between erythroblasts and fibronectin-expressing macrophages. Our findings are in agreement with a previous study that has suggested α5β1 integrin as component or modulators of the agrin signal transduction pathway.32 We have recently reported severe defects in development and functions of agrin-deficient monocytes16 and thus we speculated that the observed defects in red cell maturation might depend on the inability of agrin-deficient macrophages to support EI formation. Indeed, EI isolated from agrin-deficient mice were largely defective and the in vitro experiments confirmed a role for the agrin/DG axis in their formation. Interestingly, our experiments showed that both macrophages and red cells require agrin and its receptor for optimal EI functionality. In line with our findings, previous studies showing that the severe macrophage depletion causes a mild deficit in red cell production.19, 20
Overall, our results identify new crucial players at the erythroid niche controlling adhesion in a complex manner: the DG complex binds agrin expressed by both erythroid cells and macrophages, induces clustering and activation of the RTK EphB1 and leads to the upregulation of α5β1 integrins. More importantly, this study demonstrates a new role for agrin and DG as triggers of EphB1 activity and suggests that similar signaling processes may occur during different cell–cell interactions and may be crucial for the activity of the Eph family kinases. Whether agrin regulates Eph signaling at the neuromuscolar junction is a question that deserves investigation.
Agrin-deficient, Musk-L;Agrn−/− mice (on C57Bl/6 background) have been described elsewhere.15, 16, 18 Congenic (CD45.1) mice were used as recipients of bone marrow transplantation experiments. Procedures involving animals and their care conformed to institutional guidelines in compliance with national (4D.L. N.116, G.U., suppl. 40, 18-2-1992) and international (EEC Council Directive 86/609, OJ L 358,1,12-12-1987; National Institutes of Health Guide for the Care and Use of Laboratory Animals) law and policies. The protocol was approved by the Italian Ministry of Health on 18 June 2007 and modified by Protocol 162/2011-B. All efforts were made to minimize the number of animals used and their suffering.
Immunohistochemistry and immunofluorescence
Formalin-fixed paraffin-embedded 2-μm sections were deparaffinized, rehydrated and incubated after antigen retrieval with anti-TER-119 (BD Pharmingen, San Diego, CA, USA) and the appropriate secondary antibody. Staining was visualized by 3,3′-diaminobenzidine (Dako, Carpinteria, CA, USA) and counterstained with hematoxylin. Images were acquired with Olympus BX51 microscope (Center Valley, PA, USA). Sorted erythroid cells were fixed with 1 or 4% formaldehyde, blocked with bovine serum albumin 1% and incubated with the primary antibodies anti-agrin (gift from Prof M. Ferns, Department of Physiology and Membrane Biology, University of California, Davis School of Medicine, Davis, CA, USA), anti–α-DG (AbCam, Cambridge, UK), anti ph-DG (AbCam) and anti-EphB1 (M19; Santa Cruz Biotechnology, Dallas, TX, USA). To quantify EphB1 clusters, primary erythroid R2 cells, either untreated or anti-α-DG antibody IIH6C4 (10 ug/ml, Millipore, Billerica, MA, USA) treated, were incubated with 10 ug/ml recombinant agrin (R&D Biosystems, Minneapolis, MN, USA); after fixation in 4% PFA for 30 min, cells were incubated with anti-EphB1 (M19; Santa Cruz) and anti-goat Alexa-conjugated secondary antibody (Molecular Probes, Eugene, OR, USA). The nuclei were counterstained with Hoechst 33342 (Molecular Probes). Images were acquired by a confocal microscope Fluoview FV1000 (Olympus) with an oil immersion objective (× 40 or 60 × 1.4 NA Plan-Apochromat; Olympus) using laser excitation at 405, 488, 594 or 633 nm, and processed using Adobe Photoshop 9.0.2.
FACS and analysis
Splenic cell suspensions were obtained by manual smashing of total spleens; BM cell suspensions were obtained by flushing of multiple bones (femur, tibia, humerus, ulna) in sterile staining buffer (PBS containing 2% FCS). Cell surface stainings were performed in staining buffer for 30 min, on ice, in the dark, with the appropriate combinations of saturating concentrations of the following conjugated monoclonal antibodies (mAbs) obtained from either BD Biosciences (San Jose, CA, USA), eBioscience (San Diego, CA, USA) or BioLegend (San Diego, CA, USA): CD71 (C2), TER-119 (Ly-76), CD11b (M1/70), CD45 (30-F11), CD49e (5H10-27, CD51 (RMV-7), CD61 (2C9.G2), CD29 (HMb1-1). F4/80 (A3-1) was purchased from Serotech (Kidlington, Oxford, UK). All macrophages sorting (FACS) experiments have been performed with the exclusion of SSChigh eosinophil population. Anti-Agrin antibody (Agr 247) was purchased from AbCam and its isotype control IgG1 from Invitrogen (Carlsbad, CA, USA); both antibodies were labeled using Zenon labeling technology (Molecular Probes). Anti-α-DG antibody IIH6C4 or isotype control were purchased from Millipore and Biolegend, respectively. Rabbit anti-Integrin Beta 1 /CD29 receptor phospho-specific (THR788/789) antibody (AB1929) was purchased from Millipore. Goat anti-mouse-Alexa 647 and Goat anti-rabbit-Alexa 647, purchased from Life Technologies (Carlsbad, CA, USA), were used as secondary antibodies. For actin polimerization assay, sorted R2 cells were treated using Cytofix/Cytoperm Kit (BD Bioscience) according to the manufacturer’s instructions and then stained with Phalloidin Alexa-488 purchased from Life Technologies. For Annexin-V staining, freshly isolated cells were first stained with the appropriate mAbs, and then washed in Annexin-V binding buffer (BD Biosciences) and incubated with Annexin-V (BD Biosciences). Dead cells were excluded using 7AAD or by low angle and orthogonal light scatter. The absolute count of leukocytes was performed using TruCount tubes (BD Biosciences) according to the manufacturer’s instructions. Stained cells were analyzed with a FACSCanto I (2 lasers) or FACSCanto II (3 lasers) or LSR Fortessa (4 lasers) flow cytometer (BD Biosciences). Cell sorting experiments were performed using a FACSAria III (4 lasers; BD Biosciences).
Clusters composed of splenic macrophages and erythroblasts were isolated as already described.33 Bone marrow or splenic cell suspensions were allowed to attach to glass coverslips for 4 h 37 °C in 5% CO2. In all the reconstituted island experiments, an equal number of sorted control or Musk-LAgrn−/− Ter119+ erythroid cells were added to F4/80+ cells (i.e., 10 Ter119+ cells per F4/80+ cell). When indicated, cells were incubated with anti-α-DG antibody IIH6C4 (10 ug/ml, Millipore) or isotype control (Biolegend). Cells were fixed with formalin (4%), blocked in PBS/1% bovine serum albumin and incubated with the indicated antibodies. Confocal z axis stacks were acquired and the number of TER-119-positive cells attached to each F4/80-positive, adherent macrophage was counted in 3D reconstructions.
Mouse phospho-RTK array
Murine sorted ctrl R2 cells (5 × 106) were stimulated or not with recombinant agrin (10 μg/ml, R&D Biosystems) for 5–30 min at 37 °C, after pretreatment with anti-α-DG blocking antibody (10 μg/ml, clone IIH6C4, Millipore) when required. Cells were lysed, proteins were extracted, RTK membranes were probed and densitometric analyses were performed according to manufacturer’s instructions (R&D Systems, Catalog number ARY014).
Western blot analysis
K562 human erytholeukemic cell line maintained in RPMI 1640 medium (HEPES modification), with 10% fetal calf serum, 1% penicillin/streptomycin, 2 mM L-glutamine at 37 °C, 5% CO2 were seeded at 0.2 × 106 cells/ml for 24 h prior to agrin treatment. Cells were lysed in 1% Triton X-100, 20 mM Tris-HCl pH 8, 137 mM NaCl, 10% glycerol, 2 mM EDTA, 1 mM PMSF, 1 mM Na3VO4, 50 mM NaF, 10 mM NaPP, 5 μg/ml leupeptin, 5 μg/ml Aprotinin, containing protease inhibitors cocktail (Roche, Mannheim, Germany), with rotation at 4 °C for 15 min. DNA complexes were disrupted incubating with Benzonase nuclease (Novagen, Darmstadt, Germany) for 10 min at room temperature. Proteins (15 μg) after quantification by the BCA protein assay (Thermo Scientific, Pittisburgh, PA, USA) were resuspended in Laemmli sample buffer, separated by electrophoresis on 4–15% TGX Stain-Free precast gels (Bio-Rad, Hercules, CA, USA) and transferred on PVDF membranes (Bio-Rad). The use of Stain-Free imaging allowed the normalization of bands to the total protein loaded on the gel, without housekeeping protein normalization or stripping and reprobing. Blots were probed with the primary antibody anti-DG (phospho Y893) or anti-Eph-B1 (phospho Y317) purchased from AbCam, according to the manufacturer’s protocol and then with peroxidase-conjugated secondary antibodies (Bio-Rad). Blots were developed with Luminata Forte Western HRP substrate (Millipore) on the ChemiDoc MP System (Bio-Rad).
The human K562 erythroleukemia cell line was used for immunoprecipitation experiments. Cells were maintained in RPMI 1640 medium with 10% fetal calf serum, 1% penicillin/streptomycin, 2 mM L-glutamine at 37 °C, 5% CO2 and seeded at 0.2 × 106 cells/ml the day prior to treatment. Cells were resuspended in RPMI 1640 medium with 2% fetal calf serum and were stimulated or not with recombinant agrin (10 μg/ml, R&D Systems) for 10 min at 37 °C, after pretreatment with anti-α-DG blocking antibody (10 μg/ml, clone IIH6C4, Millipore) when required. Cells were lysed on ice for 30 min in 50 mM Tris-HCl, pH 7.4, 2% CHAPS, protease inhibitor cocktail (Roche) and incubated with 375 U/ml Benzonase Nuclease (Merck Millipore, Massachusetts, USA). Magnetic beads with coupled sheep anti-mouse IgG (Dynabeads; Life Technologies) were washed with 0.1% bovine serum albumin IgG-free (Sigma-Aldrich, St. Louis, MO, USA) in PBS. Beads were incubated for 2 h at 4 °C with anti-EphB1 (clone 5F10A4, Thermo Fisher Scientific, Pittsburgh, PA USA). Equal amounts of protein (1000 μg) were pre-cleared incubated overnight at 4 °C with primary antibody cross-linked to the beads. Cross-linking was carried out by incubation with 20 mM dimethyl pimelimidate dihydrochloride (Sigma-Aldrich) in 0.2 M triethanolamine pH 8.2 for 30 min at room temperature. As a control, murine IgG-coated beads were used in each experiment. IPed proteins were eluted in Laemmli sample buffer. Samples were separated by 4–15% TGX Stain-Free precast gels (Bio-Rad) and WB for DG (phospho Y893, AbCam) was performed. IP experiments were repeated several times on independent sample sets, with consistent results.
Statistical significance was calculated using the unpaired Student's t-test; (GraphPad Prism version 4.0). Differences were considered significant when P⩽0.05 (asterisk), very significant when P⩽0.01 (two asterisks) and extremely significant when P⩽0.001 (three asterisks).
We thank Francesco Piazza and Marco Sandri for critical reading of the MS, Steven Burden for providing mice, Chiara Buracchi and Paolo Somma for cell sorting experiments and Annarita Destro for assistance. This work was funded by the ERC AdG STePS to AV.
AA and CM conceived and designed the study and performed experiments; EL performed biochemical experiments; CS and RA performed confocal microscopy experiments; CP performed in vivo experiments; GD and AS provided suggestions and reagents; CM and AV supervised the study and wrote the manuscript; AV provided funds.
bone marrow transfer
erythropoietin-producing human hepatocellular carcinoma line
Eph family receptor interacting proteins
glyceraldehyde 3-phosphate dehydrogenase
growth factor receptor-bound protein 2
intercellular adhesion molecule 4
lipoprotein-related receptor 4
megakaryocyte erythroid progenitors
polymerase chain reaction
receptor tyrosine kinases
son of sevenless guanine nucleotide exchange factor
vascular cell adhesion molecule 1
Supplementary Information accompanies this paper on Cell Death and Differentiation website (http://www.nature.com/cdd)
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