This Month |
Featured
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Brief Communication |
Artifact-free high-density localization microscopy analysis
Preprocessing of localization microscopy datasets using Haar wavelet kernel (HAWK) analysis enables artifact-free analysis of high-density data for improved fixed and live-cell super-resolution microscopy.
- Richard J. Marsh
- , Karin Pfisterer
- & Susan Cox
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Brief Communication |
Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections
Active PSF shaping and adaptive optics are combined to enable 3D localization microscopy throughout thick tissues. The method was used to study the nanoscale architecture of amyloid fibrils in a mouse model of Alzheimer’s disease.
- Michael J. Mlodzianoski
- , Paul J. Cheng-Hathaway
- & Fang Huang
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Brief Communication |
Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.
- Francesca Pennacchietti
- , Ekaterina O. Serebrovskaya
- & Ilaria Testa
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Research Highlight |
Deep learning advances super-resolution imaging
Two approaches apply deep learning to improve single-molecule localization microscopy.
- Rita Strack
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Brief Communication |
Single-shot super-resolution total internal reflection fluorescence microscopy
Instant structured illumination and total internal reflection fluorescence microscopy are combined to carry out time-lapse super-resolution TIRF imaging at frame rates up to 100 Hz, enabling observation of fast biological processes.
- Min Guo
- , Panagiotis Chandris
- & Hari Shroff
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Article |
Self-interference 3D super-resolution microscopy for deep tissue investigations
Several methods make it possible to obtain depth information in 3D super-resolution microscopy. Here the authors exploit a self-interference phenomenon to allow deep imaging within tissue samples, as well as isotropic imaging.
- Pierre Bon
- , Jeanne Linarès-Loyez
- & Laurent Cognet
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Research Highlights |
Correlation analysis in single-molecule localization microscopy
A coordinate-based framework quantifies the correlation and interaction of biomolecules in images acquired with super-resolution microscopy techniques.
- Christian Schnell
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Brief Communication |
Real-time 3D single-molecule localization using experimental point spread functions
An open-source, real-time fitter for 3D single-molecule localization microscopy uses experimental point spread functions. This enables accurate 3D super-resolution microscopy on standard microscopes without dedicated 3D optics.
- Yiming Li
- , Markus Mund
- & Jonas Ries
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Tools in Brief |
Peptide-tag-specific nanobody for dSTORM
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Research Highlights |
Illuminating the brain's dark matter
SUSHI is a new method for imaging the extracellular space with high resolution in living brain tissue to reveal insights into the structure and dynamics of the extracellular space.
- Christian Schnell
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Methods in Brief |
Tilted light-sheet microscopy with 3D point-spread functions
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Brief Communication |
Quantitative mapping and minimization of super-resolution optical imaging artifacts
This paper reports an approach to map errors in super-resolution images, based on quantitative comparison to diffraction-limited equivalents.
- Siân Culley
- , David Albrecht
- & Ricardo Henriques
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Research Highlights |
Off-the-shelf super-resolution microscopy in whole cells
The combination of DNA-PAINT and spinning-disk confocal microscopy makes super-resolution microscopy in whole cells affordable and easy to implement.
- Christian Schnell
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Perspective |
STED super-resolved microscopy
This Perspective reviews nanoscopy via stimulated emission depletion (STED), focusing on challenges for biologists and how technical advances are helping to meet these challenges.
- Giuseppe Vicidomini
- , Paolo Bianchini
- & Alberto Diaspro
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News & Views |
Super-resolution imaging goes fast and deep
Advances in image scanning microscopy move super-resolution imaging deeper into tissues with faster visualization and finer details.
- Sam Duwé
- & Peter Dedecker
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Article |
Localization-based super-resolution imaging meets high-content screening
An automated system for data acquisition and analysis enables high-content screening localization microscopy and increases the throughput and information content of super-resolution microscopy methods such as dSTORM, DNA-PAINT and (spt)PALM.
- Anne Beghin
- , Adel Kechkar
- & Jean-Baptiste Sibarita
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Research Highlights |
From localizations to structure
Single-particle reconstruction algorithms can be used to obtain 3D structures from 2D localization microscopy images.
- Rita Strack
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Technology Feature |
Probes: FRET sensor design and optimization
Trial, error and the art of optimizing 'molecular rulers' that sense molecules or interactions.
- Vivien Marx
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Research Highlights |
Nanoscopic imaging that lasts
Spontaneously blinking membrane probes enable live-cell super-resolution imaging for up to half an hour.
- Rita Strack
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Research Highlights |
Super-resolution imaging with mCherry
A chemical caging strategy allows mCherry to be used for localization microscopy.
- Rita Strack
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Brief Communication |
Adaptive optics improves multiphoton super-resolution imaging
Adaptive optics and two-photon instant structured illumination microscopy are combined to provide improved super-resolution imaging within optically aberrating biological samples.
- Wei Zheng
- , Yicong Wu
- & Hari Shroff
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Brief Communication |
Quantitative mRNA imaging throughout the entire Drosophila brain
Improved fluorescence in situ hybridization enables smFISH in cleared whole-mount Drosophila brains with confocal microscopy; a custom Bessel beam structured illumination microscope allows single-mRNA detection across the entire brain.
- Xi Long
- , Jennifer Colonell
- & Timothée Lionnet
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Methods in Brief |
Chip-based nanoscopy
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Methods in Brief |
STED with twice the depletion
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Article |
Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution
New fluorescent biosensors enable the first super-resolution imaging of enzyme activity in live cells via fluorescence fluctuation increase by contact (FLINC).
- Gary C H Mo
- , Brian Ross
- & Jin Zhang
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Methods in Brief |
Improved 3D single-molecule imaging
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Methods in Brief |
Faster Bayesian localization microscopy
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Research Highlights |
Taking nanoscopy to the limit
MINFLUX nanoscopy can obtain 1-nm localization precision with orders of magnitude fewer emitted photons.
- Rita Strack
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Tools in Brief |
Special delivery for live-cell imaging
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Research Highlights |
Extending super-resolution's FOV
An optimized illumination strategy allows for uniform single-molecule localization microscopy over large regions.
- Rita Strack
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Correspondence |
Temporal accumulation analysis provides simplified artifact-free analysis of membrane-protein nanoclusters
- Christoph Spahn
- , Frank Herrmannsdörfer
- & Mike Heilemann
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Brief Communication |
Photometry unlocks 3D information from 2D localization microscopy data
Three-dimensional localization microscopy can yield important biological insights. A photometric approach is described that allows users to gain 3D information from existing 2D images and to improve axial resolution obtained with existing biplane setups.
- Christian Franke
- , Markus Sauer
- & Sebastian van de Linde
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Research Highlights |
Orientation mapping in super-resolution
Fluorescence polarization enables super-resolution dipole orientation mapping.
- Rita Strack
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Tools in Brief |
Monomeric near-infrared fluorescent proteins
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Correspondence |
An acquisition and analysis pipeline for scanning angle interference microscopy
- Catherine B Carbone
- , Ronald D Vale
- & Nico Stuurman
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Methods in Brief |
Fast and easy super-resolution microscopy
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Research Highlights |
Simultaneous multicolor localization microscopy
Point spread function engineering streamlines multicolor super-resolution imaging and single-particle tracking.
- Rita Strack
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Methods in Brief |
Expanding expansion microscopy
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Tools in Brief |
Super-resolution imaging in live fly larvae with RESOLFT
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Research Highlights |
Nanoscopy for the whole cell
Researchers plumb the depths of the cell with volumetric 4Pi nanoscopy.
- Rita Strack
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News & Views |
The quest for multiplexed spatially resolved transcriptional profiling
Crowded transcript signals are resolved by cross-correlation or by gel expansion microscopy.
- Carolina Wählby
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Article |
Reversible cryo-arrest for imaging molecules in living cells at high spatial resolution
Reversible cryo-arrest allows consecutive super-resolution and functional imaging of molecular patterns in the same mammalian cell. This method was used to study the evolution of receptor tyrosine kinase reaction patterns at multiple scales.
- Martin E Masip
- , Jan Huebinger
- & Philippe I H Bastiaens
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Article |
Nanoscale imaging of RNA with expansion microscopy
ExFISH extends expansion microscopy to single-molecule RNA imaging, enabling super-resolution imaging of diverse RNAs in cells and tissues on conventional microscopes. The method enables multiplexed imaging of RNA and improved RNA quantitation.
- Fei Chen
- , Asmamaw T Wassie
- & Edward S Boyden
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Brief Communication |
Varying label density allows artifact-free analysis of membrane-protein nanoclusters
PALM and STORM are powerful methods for studying membrane-protein clustering. However, fluorophore blinking can lead to miscounting artifacts. A new method shows that varying label density works for artifact-free analysis of membrane-protein nanoclusters.
- Florian Baumgart
- , Andreas M Arnold
- & Gerhard J Schütz
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Methods in Brief |
Open-source software for structured illumination