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Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate
The authors find that cotton bollworms secrete effectors into plant to weaken JA signaling via host endocytosis system while JA inhibits endocytosis to restrict effector imports. The defense and counter-defense loop between effector and JA reflects a robust arms race between plant and insect.
- Zi-Wei Yan
- , Fang-Yan Chen
- & Ying-Bo Mao
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Defense against phytopathogens relies on efficient antimicrobial protein secretion mediated by the microtubule-binding protein TGNap1
Defining plant defense machinery against pathogens is significant in cell biology and crop yield. TGNap1, a TGN and microtubule-binding protein, is required for defense and efficient anti-microbial protein secretion, linking secretion and cytoskeleton.
- Deepak D. Bhandari
- , Dae Kwan Ko
- & Federica Brandizzi
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Article
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Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence
The exocyst complex is essential for plant development. Here the authors reveal a link between the exocyst complex and the ER network in regulating the degradation of exocytic compartments, stigma senescence and flower receptivity
- Tong Zhang
- , Yifan Li
- & Pengwei Wang
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Article
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The plant unique ESCRT component FREE1 regulates autophagosome closure
Nutrient sensing impinges on the autophagosome biogenesis. Here, the authors present evidence that plant energy sensing regulates the autophagosome closure by modulating the phosphorylation status and localization of the ESCRT machinery.
- Yonglun Zeng
- , Baiying Li
- & Liwen Jiang
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Article
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Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light
Pexophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis under high-intensity light.
- Kazusato Oikawa
- , Shino Goto-Yamada
- & Mikio Nishimura
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Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking
The endocytic degradation of plasma membrane proteins can be modulated by deubiquitylating enzymes (DUBs). Here, the authors describe two plasma membrane localized Arabidopsis DUBs that can be activated by binding to anionic lipids and influence the endocytic transport of plasma membrane proteins.
- Karin Vogel
- , Tobias Bläske
- & Erika Isono
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TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy
Contact sites permit the exchange of signals and materials between organelles. Here the authors characterize a protein complex associated with an ER-mitochondrial contact site in Arabidopsis that is required for mitochondrial function and mitochondrial turnover
- Chengyang Li
- , Patrick Duckney
- & Pengwei Wang
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POD1-SUN-CRT3 chaperone complex guards the ER sorting of LRR receptor kinases in Arabidopsis
Cargo transport from the ER to the Golgi is highly selective. Here the authors identify three secretory pathway localized proteins that regulate ER sorting of receptor kinases in Arabidopsis and are required to support pollen tube growth.
- Yong Xue
- , Jiang-Guo Meng
- & Wei-Cai Yang
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Sterols are required for the coordinated assembly of lipid droplets in developing seeds
Lipid droplet biogenesis originates at the endoplasmic reticulum and is defined by a specific set of lipids and proteins. Here, the authors show that sterols play an important role in coordinating oil and oleosin biosynthesis for the formation of lipid droplets in plant leaves and seeds.
- Linhui Yu
- , Jilian Fan
- & Changcheng Xu
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Article
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Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network
Lipid composition impacts the function of cellular membranes. Here the authors show that a reduction in sphingolipid acyl-chain length promotes phosphoinositide consumption by phospholipase C at the Arabidopsis trans-Golgi network which in turn regulates sorting of the auxin efflux carrier PIN2.
- Yoko Ito
- , Nicolas Esnay
- & Yohann Boutté
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Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding
AtEH/Pan1 proteins contain two N-terminal Eps15 homology (EH) domains and are subunits of the endocytic TPLATE complex present in plants. Here, the authors combine X-ray crystallography, NMR and MD simulations with biochemical and in planta analysis to characterize the two AtEH1/Pan1 EH domains and reveal their structural differences and complementary functional roles.
- Klaas Yperman
- , Anna C. Papageorgiou
- & Daniel Van Damme
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Peroxisomes form intralumenal vesicles with roles in fatty acid catabolism and protein compartmentalization in Arabidopsis
Peroxisomes are organelles compartmentalising metabolic reactions such as the breakdown of fats, and are commonly thought of as single membrane-bound compartments. Here the authors show that Arabidopsis peroxisomes contain extensive internal vesicles that form from the bounding membrane in an ESCRT-dependent process.
- Zachary J. Wright
- & Bonnie Bartel
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The liverwort oil body is formed by redirection of the secretory pathway
Liverworts have a unique oil body organelle unrelated to lipid stores found in other eukaryotes. Here the authors show that oil body formation is analogous to that of cell plates, relying on periodic redirection of the secretory pathway and a syntaxin-1 homolog, and that oil bodies contribute to defence against herbivory.
- Takehiko Kanazawa
- , Hatsune Morinaka
- & Takashi Ueda
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SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistance
Cytokinin and auxin are two major hormonal regulators of plant growth. Here the authors identify SYAC1, a gene that is synergistically activated by the two hormones being applied together, and show that it is required for normal growth while negatively impacting pathogen resistance.
- Andrej Hurný
- , Candela Cuesta
- & Eva Benková
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Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery
Arabidopsis EH/Pan1 proteins are part of the TPLATE complex (TPC) that is required for endocytosis in plants. Here, the authors show AtEH/Pan1 proteins also act in actin-mediated autophagy, by interacting with VAP27-1 at ER-PM contact sites and recruiting TPLATE and AP-2 complex subunits, clathrin and ARP2/3/ proteins to autophagosomes.
- Pengwei Wang
- , Roman Pleskot
- & Patrick J. Hussey
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PAWH1 and PAWH2 are plant-specific components of an Arabidopsis endoplasmic reticulum-associated degradation complex
Endoplasmic reticulum (ER)-associated degradation (ERAD) removes misfolded proteins from the secretory pathway. Here the authors identify two plant-specific proteins in Arabidopsis, PAWH1 and PAWH2, that bind to and stabilise the ER-anchored ubiquitin ligase Hrd1.
- Liangguang Lin
- , Congcong Zhang
- & Jianming Li
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Systemic signaling contributes to the unfolded protein response of the plant endoplasmic reticulum
The unfolded protein response (UPR) maintains cellular function under ER stress. While typically considered cell autonomous, here Lai et al. show that in Arabidopsis the UPR also has a systemic component triggered by intercellular movement of at least one bZIP transcription factor.
- Ya-Shiuan Lai
- , Giovanni Stefano
- & Federica Brandizzi
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Article
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A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis
The ESCRT complex contributes to selective vacuolar degradation of membrane proteins. Here, the authors show that the plant-specific BRAF protein negatively regulates intraluminal vesicle formation and membrane protein degradation by preventing FREE1 binding to the ESCRT-I complex protein Vps23.
- Jinbo Shen
- , Qiong Zhao
- & Liwen Jiang
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Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi
Vacuolar sorting receptors (VSRs) are suggested to efficiently transport hydrolases by continuous cycling. Here, the authors use a nanobody-epitope interaction-based labeling approach to trace VSR recycling from the TGN/EE to the cis-Golgi and reveal ligand reloading of recycled VSRs.
- Simone Früholz
- , Florian Fäßler
- & Peter Pimpl
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Towards designer organelles by subverting the peroxisomal import pathway
Designer organelles could allow the isolation of synthetic biological pathways from endogenous components of the host cell. Here the authors engineer a peroxisomal protein import pathway orthogonal to the naturally occurring system.
- Laura L. Cross
- , Rupesh Paudyal
- & Stuart L. Warriner
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Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains
Sphingolipids in the trans-Golgi network have been implicated in polar trafficking. Here Wattelet-Boyer et al. show that hydroxylated C24- and C26-acyl-chain sphingolipids are enriched in trans-Golgi network subdomains that are critical for polar sorting of the PIN2 auxin carrier in plant cells.
- Valérie Wattelet-Boyer
- , Lysiane Brocard
- & Yohann Boutté
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Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis
Cellulose is produced in plants by cellulose synthase complexes (CSCs) that are assembled in the endomembrane system and then trafficked to the plasma membrane. Here Zhang et al. show that the Golgi-localized STELLO1 and 2 proteins are required for the proper assembly and distribution of CSCs in plant cells.
- Yi Zhang
- , Nino Nikolovski
- & Paul Dupree
Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling