Permeation and transport

  • Article
    | Open Access

    Structural studies of human Na+–taurocholate co-transporting polypeptide in complex with nanobodies reveal mechanisms for bile salts transport and HBV recognition involving an open-pore intermediate state.

    • Kapil Goutam
    • , Francesco S. Ielasi
    •  & Nicolas Reyes
  • Article
    | Open Access

    Cryo-EM structures of AMPA receptor with the subunit γ2 in non-desensitizing conditions at low glutamate concentrations disprove the one-to-one link between the number of glutamate-bound subunits and ionotropic glutamate receptor conductance.

    • Maria V. Yelshanskaya
    • , Dhilon S. Patel
    •  & Alexander I. Sobolevsky
  • Article |

    Cryo-electron microscopy structures of the sodium–glucose cotransporter SGLT1 and a related transporter SMCT1 define the architecture of this protein family and provide insights into substrate binding and transport function.

    • Lei Han
    • , Qianhui Qu
    •  & Liang Feng
  • Article |

    Cryo-electron microscopy studies show that dynamic coordination of Na+ in the ion channel of Dispatched homologue 1 and the transmembrane Na+ gradient have key roles in exporting lipid-modified Hedgehog protein signal.

    • Qianqian Wang
    • , Daniel E. Asarnow
    •  & Philip A. Beachy
  • Article |

    A structure of the sperm-specific CatSper complex features a number of additional components; together, these components and the CatSper complex are termed the CatSpermasome.

    • Shiyi Lin
    • , Meng Ke
    •  & Jianping Wu
  • Article |

    Cryo-electron microscopy structures of PCFT in a substrate-free state and bound to the antifolate drug pemetrexed provide insights into how this protein recognizes folates and mediates their transport into cells.

    • Joanne L. Parker
    • , Justin C. Deme
    •  & Simon Newstead
  • Article |

    Structures of the human sodium-dependent citrate transporter NaCT in complexes with citrate or a small-molecule inhibitor reveal how the inhibitor—which binds to the same site as citrate—arrests the transport cycle of NaCT.

    • David B. Sauer
    • , Jinmei Song
    •  & Da-Neng Wang
  • Article |

    Partial structures are reported for Cav3.1 both on its own and in complex with a phase II drug candidate for seizure and neuropathic pain; this presents the overall architecture of T-type Ca2+ channels, insights into their gating and permeation mechanisms, and how they are modulated by small-molecule blockers.

    • Yanyu Zhao
    • , Gaoxingyu Huang
    •  & Nieng Yan
  • Article |

    The cryo-EM structure of the zebrafish cation–chloride cotransporter NKCC1 reveals the domain organization, ion translocation pathway, ion-binding sites and key residues for binding activity, providing insights into the activity of this family of transporter proteins with key roles in physiology.

    • Thomas A. Chew
    • , Benjamin J. Orlando
    •  & Liang Feng
  • Article |

    The regulatory mechanism through which ryanodine receptor 2 is modulated by calmodulin is revealed through structural determination of ryanodine receptor 2 under eight conditions.

    • Deshun Gong
    • , Ximin Chi
    •  & Nieng Yan
  • Letter |

    Cryo-electron microscopy of the serotonin 5-HT3 receptor in complex with various ligands yields four distinct structures, capturing serotonin binding in detail and increasing understanding of the gating mechanism of the receptor.

    • Lucie Polovinkin
    • , Ghérici Hassaine
    •  & Hugues Nury
  • Article |

    The structure of a homomeric channel of subunit A of leucine-rich repeat-containing protein 8 (LRRC8) determined by cryo-electron microscopy and X-ray crystallography reveals the basis for anion selectivity.

    • Dawid Deneka
    • , Marta Sawicka
    •  & Raimund Dutzler
  • Letter |

    X-ray and cryo-electron microscopy structures of the acid-sensing ion channel ASIC1a reveal the molecular mechanisms of channel gating and desensitization.

    • Nate Yoder
    • , Craig Yoshioka
    •  & Eric Gouaux
  • Letter |

    The cryo-electron microscopy structure of the calcium channel TRPV6 in its open and closed states demonstrates a novel gating mechanism involving an alanine hinge.

    • Luke L. McGoldrick
    • , Appu K. Singh
    •  & Alexander I. Sobolevsky
  • Letter |

    The structure of mouse transient receptor potential mucolipin 1 (TRPML1), a cation channel located within endosomal and lysosomal membranes, is resolved using single-particle electron cryo-microscopy.

    • Qingfeng Chen
    • , Ji She
    •  & Youxing Jiang
  • Article |

    High-resolution structures of the thermostabilized human excitatory amino acid transporter EAAT1, alone or in association with its substrate or small molecule inhibitors, reveal architectural features of human SLC1 transporters and an allosteric mechanism of inhibition.

    • Juan C. Canul-Tec
    • , Reda Assal
    •  & Nicolas Reyes
  • Letter |

    The X-ray crystal structure of NapA, a Na+/H+ antiporter from Thermus thermophilus, in an active, outward-facing state is reported; comparisons to the structure of a related transporter in a low pH/inactivated, inward-facing state show the conformational changes that occur when the membrane protein moves from an inward-facing to an outward-facing state, suggesting that Na+/H+ antiporters operate by a two-domain rocking bundle model.

    • Chiara Lee
    • , Hae Joo Kang
    •  & David Drew
  • Article |

    Here it is shown that ion flux through the TrkH–TrkA complex is upregulated by ATP and downregulated by ADP; solving the X-ray crystal structures of the tetrameric TrkA ring in the absence and presence of TrkH suggests a mechanism by which ATP-induced conformational changes in TrkA augment the activity of TrkH.

    • Yu Cao
    • , Yaping Pan
    •  & Ming Zhou
  • Letter |

    STIM1-mediated gating of CRAC channels occurs through a mechanism in which ion selectivity and gating are closely coupled, and the residue V102 is identified as a candidate for the channel gate.

    • Beth A. McNally
    • , Agila Somasundaram
    •  & Murali Prakriya