Parasite genetics

  • Article | | open

    Host-parasite interactions during the exoerythrocytic stage of Plasmodium infection remains poorly understood. Using dual RNA-Seq, the authors show that human mucosal immunity protein mucin-13 is upregulated during Plasmodium hepatic-stage infection and marks infected cells independent of tested Plasmodium species.

    • Gregory M. LaMonte
    • , Pamela Orjuela-Sanchez
    • , Jaeson Calla
    • , Lawrence T. Wang
    • , Shangzhong Li
    • , Justine Swann
    • , Annie N. Cowell
    • , Bing Yu Zou
    • , Alyaa M. Abdel-Haleem Mohamed
    • , Zaira Hellen Villa Galarce
    • , Marta Moreno
    • , Carlos Tong Rios
    • , Joseph M. Vinetz
    • , Nathan Lewis
    •  & Elizabeth A. Winzeler
  • Article | | open

    Increasing resistance of Plasmodium falciparum strains to piperaquine (PPQ) in Southeast Asia is of concern and resistance mechanisms are incompletely understood. Here, Ross et al. show that mutations in the P. falciparum chloroquine resistance transporter are rapidly increasing in prevalence in Cambodia and confer resistance to PPQ.

    • Leila S. Ross
    • , Satish K. Dhingra
    • , Sachel Mok
    • , Tomas Yeo
    • , Kathryn J. Wicht
    • , Krittikorn Kümpornsin
    • , Shannon Takala-Harrison
    • , Benoit Witkowski
    • , Rick M. Fairhurst
    • , Frederic Ariey
    • , Didier Menard
    •  & David A. Fidock
  • Article | | open

    Plasmodium vivax incidence in Malaysia has declined markedly over the last decade, despite evidence of chloroquine resistance. Here, Auburn et al. compare population structure of P. vivax in Malaysia to regions with intermediate and high transmission and identify genetic regions under putative selection.

    • Sarah Auburn
    • , Ernest D. Benavente
    • , Olivo Miotto
    • , Richard D. Pearson
    • , Roberto Amato
    • , Matthew J. Grigg
    • , Bridget E. Barber
    • , Timothy William
    • , Irene Handayuni
    • , Jutta Marfurt
    • , Hidayat Trimarsanto
    • , Rintis Noviyanti
    • , Kanlaya Sriprawat
    • , Francois Nosten
    • , Susana Campino
    • , Taane G. Clark
    • , Nicholas M. Anstey
    • , Dominic P. Kwiatkowski
    •  & Ric N. Price
  • Article | | open

    Many strains of Plasmodium differ in virulence, but factors that control these distinctions are not known. Here the authors comparatively map virulence loci using the offspring from a P. yoelii YM and N67 genetic cross, and identify a putative HECT E3 ubiquitin ligase that may explain the variance.

    • Sethu C. Nair
    • , Ruixue Xu
    • , Sittiporn Pattaradilokrat
    • , Jian Wu
    • , Yanwei Qi
    • , Martine Zilversmit
    • , Sundar Ganesan
    • , Vijayaraj Nagarajan
    • , Richard T. Eastman
    • , Marlene S. Orandle
    • , John C. Tan
    • , Timothy G. Myers
    • , Shengfa Liu
    • , Carole A. Long
    • , Jian Li
    •  & Xin-zhuan Su
  • Article | | open

    Antimalarial chemotherapy relies on combination therapies (ACTs) consisting of an artemisinin derivative and a partner drug. Here, the authors study the effects of globally prevalent mutations in a multidrug resistance transporter (PfMDR1) on the parasite’s susceptibility to ACT drugs.

    • M. Isabel Veiga
    • , Satish K. Dhingra
    • , Philipp P. Henrich
    • , Judith Straimer
    • , Nina Gnädig
    • , Anne-Catrin Uhlemann
    • , Rowena E. Martin
    • , Adele M. Lehane
    •  & David A. Fidock
  • Article | | open

    Current strategies for regulatory control of gene expression are orthogonal to the host organism mechanisms. Here the authors demonstrate an RNA aptamer controlled system integrated into native regulatory pathways in the parasite Plasmodium falciparum.

    • Suresh M. Ganesan
    • , Alejandra Falla
    • , Stephen J. Goldfless
    • , Armiyaw S. Nasamu
    •  & Jacquin C. Niles
  • Article | | open

    The functions of many putative membrane transport proteins of malaria parasites are unknown. Here, Kenthirapalan et al. use mutant strains carrying targeted gene deletions to study the functions of 35 such proteins during the life cycle of Plasmodium berghei in mosquito and mouse hosts.

    • Sanketha Kenthirapalan
    • , Andrew P. Waters
    • , Kai Matuschewski
    •  & Taco W. A. Kooij
  • Article | | open

    Toxoplasma gondii is a parasite that causes zoonotic infections in humans. Here, the authors identify tandem amplification and diversification of secretory pathogenesis determinants in the T. gondii genome and show that clade-specific inheritance of conserved haploblocks enriched for these determinants shapes population structure.

    • Hernan Lorenzi
    • , Asis Khan
    • , Michael S. Behnke
    • , Sivaranjani Namasivayam
    • , Lakshmipuram S. Swapna
    • , Michalis Hadjithomas
    • , Svetlana Karamycheva
    • , Deborah Pinney
    • , Brian P. Brunk
    • , James W. Ajioka
    • , Daniel Ajzenberg
    • , John C. Boothroyd
    • , Jon P. Boyle
    • , Marie L. Dardé
    • , Maria A. Diaz-Miranda
    • , Jitender P. Dubey
    • , Heather M. Fritz
    • , Solange M. Gennari
    • , Brian D. Gregory
    • , Kami Kim
    • , Jeroen P. J. Saeij
    • , Chunlei Su
    • , Michael W. White
    • , Xing-Quan Zhu
    • , Daniel K. Howe
    • , Benjamin M. Rosenthal
    • , Michael E. Grigg
    • , John Parkinson
    • , Liang Liu
    • , Jessica C. Kissinger
    • , David S. Roos
    •  & L. David Sibley
  • Article | | open

    Antigens encoded by var genes are major virulence factors of the human malaria parasite Plasmodium falciparum. Here, Larremore et al. identify var-like genes in distantly related Plasmodium species infecting African apes, indicating that these genes already existed in an ancestral ape parasite many millions of years ago.

    • Daniel B. Larremore
    • , Sesh A. Sundararaman
    • , Weimin Liu
    • , William R. Proto
    • , Aaron Clauset
    • , Dorothy E. Loy
    • , Sheri Speede
    • , Lindsey J. Plenderleith
    • , Paul M. Sharp
    • , Beatrice H. Hahn
    • , Julian C. Rayner
    •  & Caroline O. Buckee
  • Article | | open

    Screening for new anthelmintic compounds that are active against parasitic nematodes is costly and labour intensive. Here, the authors use the non-parasitic nematode Caenorhabditis elegans to identify 30 anthelmintic lead compounds in an effective and cost-efficient manner.

    • Andrew R. Burns
    • , Genna M. Luciani
    • , Gabriel Musso
    • , Rachel Bagg
    • , May Yeo
    • , Yuqian Zhang
    • , Luckshika Rajendran
    • , John Glavin
    • , Robert Hunter
    • , Elizabeth Redman
    • , Susan Stasiuk
    • , Michael Schertzberg
    • , G. Angus McQuibban
    • , Conor R. Caffrey
    • , Sean R. Cutler
    • , Mike Tyers
    • , Guri Giaever
    • , Corey Nislow
    • , Andy G. Fraser
    • , Calum A. MacRae
    • , John Gilleard
    •  & Peter J. Roy
  • Article | | open

    Lucilia cuprina is a parasitic blowfly of major economic importance worldwide that feeds on the tissues of animals such as sheep. Here, the authors sequence the genome of L. cuprina and provide insights into the fly’s molecular biology, interactions with the host animal and insecticide resistance.

    • Clare A. Anstead
    • , Pasi K. Korhonen
    • , Neil D. Young
    • , Ross S. Hall
    • , Aaron R. Jex
    • , Shwetha C. Murali
    • , Daniel S.T. Hughes
    • , Siu F. Lee
    • , Trent Perry
    • , Andreas J. Stroehlein
    • , Brendan R.E. Ansell
    • , Bert Breugelmans
    • , Andreas Hofmann
    • , Jiaxin Qu
    • , Shannon Dugan
    • , Sandra L. Lee
    • , Hsu Chao
    • , Huyen Dinh
    • , Yi Han
    • , Harsha V. Doddapaneni
    • , Kim C. Worley
    • , Donna M. Muzny
    • , Panagiotis Ioannidis
    • , Robert M. Waterhouse
    • , Evgeny M. Zdobnov
    • , Peter J. James
    • , Neil H. Bagnall
    • , Andrew C. Kotze
    • , Richard A. Gibbs
    • , Stephen Richards
    • , Philip Batterham
    •  & Robin B. Gasser
  • Article | | open

    Toxocara canis is a zoonotic parasite of major worldwide socioeconomic importance. Here, the authors sequence the genome and transcriptome of T. canis, and highlight potential mechanisms involved in development and host–parasite interactions that could support the pursuit of new drug interventions.

    • Xing-Quan Zhu
    • , Pasi K. Korhonen
    • , Huimin Cai
    • , Neil D. Young
    • , Peter Nejsum
    • , Georg von Samson-Himmelstjerna
    • , Peter R. Boag
    • , Patrick Tan
    • , Qiye Li
    • , Jiumeng Min
    • , Yulan Yang
    • , Xiuhua Wang
    • , Xiaodong Fang
    • , Ross S. Hall
    • , Andreas Hofmann
    • , Paul W. Sternberg
    • , Aaron R. Jex
    •  & Robin B. Gasser
  • Article | | open

    The factors that modulate growth rate of the human malaria parasite Plasmodium falciparum remain poorly understood. Here Scherf and collaborators demonstrate that the Plasmodium sirtuin PfSir2a regulates the transcription of ribosomal DNA, thereby modulating parasite proliferation rate and virulence.

    • Liliana Mancio-Silva
    • , Jose Juan Lopez-Rubio
    • , Aurélie Claes
    •  & Artur Scherf