Multiphoton microscopy articles within Nature Communications

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  • Article
    | Open Access

    Bioimaging with photocontrol and multiplexing capability is vital for studying cellular interactions and dynamics, but multiplexed stimulated Raman scattering (SRS) imaging with reversible photocontrol is elusive. Here, the authors report SRS microscopy with Carbow-switch enabling multiplexed SRS imaging and tracking in live cells with reversible photocontrol and high spatiotemporal selectivity.

    • Yueli Yang
    • , Xueyang Bai
    •  & Fanghao Hu

  • Article
    | Open Access

    The GRIN lenses widely used for deep brain functional imaging suffer from a small measurement field of view due to strong fourth-order astigmatism. Here the authors report Geometric Transformation Adaptive Optics (GTAO) that corrects field-dependent astigmatism and enables large-volume in vivo imaging of deep mouse brain through 0.5 mm GRIN lenses.

    • Yuting Li
    • , Zongyue Cheng
    •  & Meng Cui

  • Article
    | Open Access

    Brain morphology is complex, heterogenous and miniaturized—and notoriously difficult to visualize. Dembitskaya et al. show how fluorescence ‘shadow imaging’ gives detailed and comprehensive access to the cellular architecture of the mouse brain in vivo.

    • Yulia Dembitskaya
    • , Andrew K. J. Boyce
    •  & U. Valentin Nägerl

  • Article
    | Open Access

    Coherent Stokes Raman scattering (CSRS) has never been explored previously for chemical imaging due to a strong fluorescence background. Here, the authors demonstrate the first fluorescence-free CSRS laser scanning microscope and predict CSRS’ unique backscattering properties.

    • Sandro Heuke
    •  & Hervé Rigneault

  • Article
    | Open Access

    Current holographic approaches for neuronal stimulation have limitations in their temporal resolution and the number of targeted neurons. Here, the authors demonstrate an approach for ultra-fast holographic light targeting which, combined with optogenetics, enables sub-millisecond control of sequential neuronal activation and high throughput simultaneous multicell illumination.

    • Giulia Faini
    • , Dimitrii Tanese
    •  & Valentina Emiliani

  • Article
    | Open Access

    The acquisition speed of two-photon fiberscopes is currently suboptimal. Here the authors report advances, including a high-speed scanner and down-sampling scheme as well as a two-stage deep learning (DL) algorithm, to allow high-speed, high-resolution imaging in freely moving mice.

    • Honghua Guan
    • , Dawei Li
    •  & Xingde Li

  • Article
    | Open Access

    Bone remodeling involves a switch between bone formation and resorption, but the mechanisms is unclear. Here, the authors show that intercellular communication via extracellular vesicles secreted by mature osteoblasts is a key factor for the switching, via a microRNA-mediated mechanism.

    • Maki Uenaka
    • , Erika Yamashita
    •  & Masaru Ishii

  • Article
    | Open Access

    Functional brain imaging with two-photon microscopy is limited by a tradeoff between imaging area and acquisition speed. Here, the authors present Quadroscope, a flexible microscope which allows for simultaneous video rate acquisition of four independently targetable brain regions across 5 mm.

    • Mitchell Clough
    • , Ichun Anderson Chen
    •  & Jerry L. Chen

  • Article
    | Open Access

    The authors develop a method to build Manhattan Raman Scattering (MARS) probes based on different core atoms, conjugation ring numbers, and stable isotope substitutions. A quantitative model predicts vibrational frequencies of MARS dyes from structures, which are used in supermultiplexed vibrational imaging.

    • Yupeng Miao
    • , Naixin Qian
    •  & Wei Min

  • Article
    | Open Access

    Currently, bidirectional control of activity in the same neurons in the same experiment is difficult. Here the authors report a Bidirectional Pair of Opsins for Light-induced Excitation and Silencing, BiPOLES, which they use in a range of organisms including worms, fruit flies, mice and ferrets.

    • Johannes Vierock
    • , Silvia Rodriguez-Rozada
    •  & J. Simon Wiegert

  • Article
    | Open Access

    Wavefront shaping is used to overcome scattering in biological tissues during imaging, but determining the compensation is slow. Here, the authors use holographic phase stepping interferometry, where new phase information is updated after each measurement, enabling fast improvement of the wavefront correction.

    • Molly A. May
    • , Nicolas Barré
    •  & Alexander Jesacher

  • Article
    | Open Access

    Probes with reversible fluorescence are useful in super-resolution microscopy, but lack sufficient chemical specificity. Here, the authors engineer alkyne tagged diarylethene to realize photo-switchable stimulated Raman scattering probes with high chemical resolution, for applications in living cells.

    • Jianpeng Ao
    • , Xiaofeng Fang
    •  & Minbiao Ji

  • Article
    | Open Access

    Multiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.

    • Henry Pinkard
    • , Hratch Baghdassarian
    •  & Laura Waller

  • Article
    | Open Access

    Monitoring hemodynamics in the brain is important in understanding medical imaging data and mechanisms of disease. Here the authors use high-throughput two-photon microscopy with an axially-extended Bessel focus to measure vessel size and blood flow down to capillary scale in the awake mouse brain.

    • Jiang Lan Fan
    • , Jose A. Rivera
    •  & Na Ji

  • Article
    | Open Access

    Spectral phasor analysis allows unmixing fluorescence microscopy images, but it requires user involvement and has a limited number of labels that can be analyzed and displayed. Here the authors present a semi-automated solution to visualise multiple spectral components of hyperspectral fluorescence images, simultaneously.

    • Wen Shi
    • , Daniel E. S. Koo
    •  & Francesco Cutrale

  • Article
    | Open Access

    Anatomical brain atlases elucidate the anatomical and functional organisation across species but different atlases have conflicting anatomical border and 3D coordinates. The authors integrated two atlases into a unified and highly segmented anatomical labelling system of the mouse brain.

    • Uree Chon
    • , Daniel J. Vanselow
    •  & Yongsoo Kim

  • Article
    | Open Access

    Super-resolution microscopy is a valuable tool in bioimaging, but often requires complex systems or post-processing. Here, the authors present super-linear excitation-emission (SEE) microscopy, which overcomes these limitations by taking advantage of markers with super-linear dependence between emission and excitation power.

    • Denitza Denkova
    • , Martin Ploschner
    •  & James A. Piper

  • Article
    | Open Access

    Multicolour images are difficult to acquire with large-scale microscopy approaches. Here the authors present a microtome-assisted microscope capable of trichromatic two-photon excitation and label-free nonlinear modalities based on wavelength mixing, and use it to analyze astrocyte morphology and neuronal projections in thick brain samples.

    • Lamiae Abdeladim
    • , Katherine S. Matho
    •  & Emmanuel Beaurepaire

  • Article
    | Open Access

    Imaging the mouse brain using glass cranial windows has limitations in terms of flexibility and long-term imaging. Here the authors engineer transparent polymer skulls that can fit various skull morphologies and can be implanted for over 300 days, enabling simultaneous high resolution brain imaging and electrophysiology across large cortical areas.

    • Leila Ghanbari
    • , Russell E. Carter
    •  & Suhasa B. Kodandaramaiah

  • Article
    | Open Access

    Recording the activity of neurons over large brain regions requires expanding the field of view of the optics without losing on spatial and temporal resolution. Here, the authors report a micro-opto-mechanical device that enables two-photon imaging across distant motor areas around 6 mm apart in the mouse.

    • Shin-Ichiro Terada
    • , Kenta Kobayashi
    •  & Masanori Matsuzaki

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