Multienzyme complexes articles within Nature

Featured

• Article | 20 July 2022

  Membrane-anchored HDCR nanowires drive hydrogen-powered CO₂ fixation

  The cryo-electron microscopy structure of the filamentous hydrogen-dependent CO₂ reductase (HDCR) enzyme from Thermoanaerobacter kivui, together with enzymatic analysis and in situ cryo-electron tomography, provides insight into the high catalytic activity of HDCR.

  • Helge M. Dietrich
  • , Ricardo D. Righetto
  •  & Jan M. Schuller

• Article | 13 July 2022

  Structure of Tetrahymena telomerase-bound CST with polymerase α-primase

  Cryo-electron microscopy structures of Tetrahymena thermophila telomerase-bound Ctc1–Stn1–Ten1 and DNA polymerase α–primase provide insights into the molecular mechanisms underlying telomere replication and maintenance.

  • Yao He
  • , He Song
  •  & Juli Feigon

• Article | 29 September 2021

  Mechanism for Cas4-assisted directional spacer acquisition in CRISPR–Cas

  Structures of the Cas4–Cas1–Cas2 complex from Geobacter sulfurreducens show that a 3′-overhang in the protospacer adjacent motif is required for complex assembly and spacer insertion into the CRISPR array.

  • Chunyi Hu
  • , Cristóbal Almendros
  •  & Ailong Ke

• Letter | 13 June 2018

  Structural basis for regulation of human acetyl-CoA carboxylase

  Cryo-electron microscopy studies of distinct, catalytically active and inactive filaments of human acetyl-CoA carboxylase 1 reveal the structural basis of its regulation.

  • Moritz Hunkeler
  • , Anna Hagmann
  •  & Timm Maier

• Article | 01 November 2017

  Structures of transcription pre-initiation complex with TFIIH and Mediator

  Cryo-electron microscopy structures of the yeast pre-initiation complex (PIC) and its complex with core Mediator provide insights into the opening of promoter DNA and the initiation of transcription.

  • S. Schilbach
  • , M. Hantsche
  •  & P. Cramer

• Letter | 27 March 2013

  A solution to release twisted DNA during chromosome replication by coupled DNA polymerases

  During chromosome replication, coupled polymerases introduce topological changes that cause lower processivity and transient lagging-strand polymerase dissociation from DNA.

  • Isabel Kurth
  • , Roxana E. Georgescu
  •  & Mike E. O'Donnell

• Article | 07 August 2011

  Structure of the membrane domain of respiratory complex I

  • Rouslan G. Efremov
  •  & Leonid A. Sazanov

• Letter | 16 June 2010

  Members of the H3K4 trimethylation complex regulate lifespan in a germline-dependent manner in C. elegans

  It is shown here that the methylation of histone proteins regulates lifespan in Caenorhabditis elegans. Deficiencies in members of the ASH-2 complex, which trimethylates histone H3 at lysine 4 (H3K4), extend worm lifespan. Meanwhile, the H3K4 demethylase RBR-2 is required for normal lifespan. These findings are consistent with the idea that an excess of H3K4 trimethylation reduces longevity. The extension of lifespan caused by ASH-2 deficiency requires an intact adult germline and the continuous production of mature eggs.

  • Eric L. Greer
  • , Travis J. Maures
  •  & Anne Brunet

• Article | 27 May 2010

  The architecture of respiratory complex I

  Complex I is an enzyme of the respiratory chain, and is crucial to cellular energy production: it couples electron transfer between NADH and quinine to proton translocation. Here, structures are presented of the membrane domain of complex I from Escherichia coli, and of the entire complex I from Thermus thermophilus. It is proposed that conformational changes at the interface of the two main domains drive a particular 110-Å-long a-helix in a piston-like motion, tilting nearby transmembrane helices and causing proton translocation.

  • Rouslan G. Efremov
  • , Rozbeh Baradaran
  •  & Leonid A. Sazanov

• News & Views | 26 May 2010

  Piston drives a proton pump

  The membrane-spanning enzyme known as complex I couples the movement of electrons to that of protons as a way of converting energy. Crystal structures suggest how electron transfer drives proton pumping from afar.

  • Tomoko Ohnishi