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Article
| Open Access
A microbial supply chain for production of the anti-cancer drug vinblastine
De novo microbial biosynthesis of vindoline and catharanthine using a highly engineered yeast and in vitro chemical coupling to vinblastine is carried out, positioning yeast as a scalable platform to produce many monoterpene indole alkaloids.
- Jie Zhang
- , Lea G. Hansen
- & Jay D. Keasling
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Article |
Biosynthesis of medicinal tropane alkaloids in yeast
The alkaloid drugs hyoscyamine and scopolamine are synthesized from sugars and amino acids in yeast, using 26 genes from yeast, plants, bacteria and animals, protein engineering and a vacuole transporter to enable functional expression of a key acyltransferase.
- Prashanth Srinivasan
- & Christina D. Smolke
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Letter |
Complete biosynthesis of cannabinoids and their unnatural analogues in yeast
Genetic engineering of yeast enables the production of cannabinoids and cannabinoid analogues from the simple sugar galactose, without the need to cultivate Cannabis.
- Xiaozhou Luo
- , Michael A. Reiter
- & Jay D. Keasling
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Letter |
Optogenetic regulation of engineered cellular metabolism for microbial chemical production
Finely tuned optogenetic control of engineered Saccharomyces cerevisiae enhances the biosynthesis of valuable products such as isobutanol in laboratory-scale fermenters.
- Evan M. Zhao
- , Yanfei Zhang
- & José L. Avalos
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Letter |
Rewriting yeast central carbon metabolism for industrial isoprenoid production
Yeast central carbon metabolism has been engineered to achieve a more efficient isoprenoid biosynthesis pathway, an advance that brings commodity-scale production of such compounds a step closer.
- Adam L. Meadows
- , Kristy M. Hawkins
- & Annie E. Tsong
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Letter |
Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform
Saccharomyces cerevisiae bearing engineered alginate and mannitol catabolic pathways can ferment sugars from brown macroalgae to produce ethanol, potentially allowing the use of brown macroalgae as a viable feedstock for the production of biofuels and renewable chemicals.
- Maria Enquist-Newman
- , Ann Marie E. Faust
- & Yasuo Yoshikuni
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Letter |
Synthetic non-oxidative glycolysis enables complete carbon conservation
A non-oxidative, cyclic pathway—termed non-oxidative glycolysis—is designed and constructed that enables complete carbon conservation in sugar catabolism to acetyl-coenzyme A, and can be used to achieve a 100% carbon yield to fuels and chemicals.
- Igor W. Bogorad
- , Tzu-Shyang Lin
- & James C. Liao
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Letter |
Microbial production of short-chain alkanes
Microbes have already been engineered to produce diesel fuels, and now the microbial production of components of petrol (gasoline) including short-chain alkanes has been achieved using Escherichia coli strains metabolically engineered with components of fatty acid biosynthesis pathways.
- Yong Jun Choi
- & Sang Yup Lee
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Letter |
High-level semi-synthetic production of the potent antimalarial artemisinin
Saccharomyces cerevisiae is engineered to produce high concentrations of artemisinic acid, a precursor of the artemisinin used in combination therapies for malaria treatment; an efficient and practical chemical process to convert artemisinic acid to artemisinin is also developed.
- C. J. Paddon
- , P. J. Westfall
- & J. D. Newman
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Review Article |
Microbial engineering for the production of advanced biofuels
- Pamela P. Peralta-Yahya
- , Fuzhong Zhang
- & Jay D. Keasling
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Research Highlights |
Two-in-one biofuel maker
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Letter |
Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
- Clementina Dellomonaco
- , James M. Clomburg
- & Ramon Gonzalez
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Research Highlights |
Bacterial chemical factories
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Letter |
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
The increasing cost of energy and concerns about the environment have emphasized the need to find new sources of fuel, with the microbial production of high-energy fuels a promising approach. Here, Escherichia coli is engineered to produce more complex biofuels — fatty esters (biodiesel), fatty alcohols and waxes — directly from simple sugars. Some cells are further engineered to express hemicellulases, a step towards producing these compounds directly from hemicellulose.
- Eric J. Steen
- , Yisheng Kang
- & Jay D. Keasling
Complete integration of carbene-transfer chemistry into biosynthesis