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| Open AccessSpecific stereochemistry of OP-1074 disrupts estrogen receptor alpha helix 12 and confers pure antiestrogenic activity
Estrogen receptor alpha (ERα) plays critical roles in the etiology and treatment of breast cancer. Here the authors synthesize benzopyrans with variable side chains to identify antiestrogenic compounds and characterize OP-1074, a compound that exhibits pure antiestrogenic activity by inducing the degradation of ERα and possesses greater potency than tamoxifen or fulvestrant in a xenograft model.
- S. W. Fanning
- , L. Hodges-Gallagher
- & P. J. Kushner
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Article
| Open AccessDual-functional peptide with defective interfering genes effectively protects mice against avian and seasonal influenza
A limited number of therapeutics is available to treat influenza A virus (IAV) infections. Here, the authors show that defective interfering genes, delivered with a dual-functional peptide that enables intracellular accumulation and prevents endosomal acidification, inhibit IAV replication in vitro and in vivo.
- Hanjun Zhao
- , Kelvin K. W. To
- & Kwok-Yung Yuen
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Article
| Open AccessTransmission-clearance trade-offs indicate that dengue virulence evolution depends on epidemiological context
Theory predicts that pathogens will evolve towards intermediate virulence, yet the necessary trade-offs invoked by this theory have rarely been demonstrated empirically. Here, the authors show that dengue virus dynamics exhibit a trade-off between transmission and clearance rates.
- Rotem Ben-Shachar
- & Katia Koelle
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Article
| Open AccessGenetic inactivation of ANGPTL4 improves glucose homeostasis and is associated with reduced risk of diabetes
Genetic variation in ANGPTL4 is associated with lipid traits. Here, the authors find that predicted loss-of-function variants in ANGPTL4 are associated with glucose homeostasis and reduced risk of type 2 diabetes and that Angptl4−/− mice on a high-fat diet show improved insulin sensitivity.
- Viktoria Gusarova
- , Colm O’Dushlaine
- & Jesper Gromada
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Article
| Open AccessHydrophobic pore gates regulate ion permeation in polycystic kidney disease 2 and 2L1 channels
Mutations in the cation channel PKD2 cause human autosomal dominant polycystic kidney disease but its channel function and gating mechanism are poorly understood. Here authors study PKD2 using electrophysiology and cryo-EM, which identifies hydrophobic gates and proposes a gating mechanism for PKD2.
- Wang Zheng
- , Xiaoyong Yang
- & Xing-Zhen Chen
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Article
| Open AccessThe TRPV4 channel links calcium influx to DDX3X activity and viral infectivity
The ion channel TRPV4 senses many environmental cues, but its role in virus infection is not known. Here, Doñate-Macián et al. show that Zika virus induces TRPV4-mediated Ca2+ influx into cells, resulting in the nuclear accumulation of the DDX3X RNA helicase, which increases virus replication.
- P. Doñate-Macián
- , J. Jungfleisch
- & M. A. Valverde
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Article
| Open Accessα-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson’s disease
How toxic aggregated forms of α-synuclein lead to neurodegeneration is unclear. Here authors use biophysical and cellular imaging methods to show that specific oligomers of α-synuclein exert effects on mitochondria to induce opening of the permeability transition pore, leading to cell death in Parkinson’s disease.
- Marthe H. R. Ludtmann
- , Plamena R. Angelova
- & Sonia Gandhi
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Article
| Open AccessA multiethnic genome-wide association study of primary open-angle glaucoma identifies novel risk loci
Primary open-angle glaucoma (POAG) leads to progressive vision loss. Here, Choquet et al. perform genome-wide association analysis for POAG in a multi-ethnic cohort, identify a total of nine novel genetic loci and show relevant function of FMNL2 and LMX1B using cell line and mouse experiments.
- Hélène Choquet
- , Seyyedhassan Paylakhi
- & Eric Jorgenson
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Article
| Open AccessVariations in Dysbindin-1 are associated with cognitive response to antipsychotic drug treatment
Patients with schizophrenia show varied response to antipsychotics. Here, the authors demonstrate in patients under antipsychotics treatment that a haplotype associated with lower dysbindin-1 expression correlated with better executive functions, providing further mechanistic support from mouse models.
- Diego Scheggia
- , Rosa Mastrogiacomo
- & Francesco Papaleo
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Article
| Open AccessFine-mapping of prostate cancer susceptibility loci in a large meta-analysis identifies candidate causal variants
Prostate cancer (PrCa) involves a large heritable genetic component. Here, the authors perform multivariate fine-mapping of known PrCa GWAS loci, identifying variants enriched for biological function, explaining more familial relative risk, and with potential application in clinical risk profiling.
- Tokhir Dadaev
- , Edward J. Saunders
- & Zsofia Kote-Jarai
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Article
| Open AccessAnalysis of 3800-year-old Yersinia pestis genomes suggests Bronze Age origin for bubonic plague
Yersinia pestis has caused infections (plague) in humans since the Early Bronze Age (5000 years ago). Here, Spyrou et al. reconstruct Y. pestis genomes from Late Bronze Age individuals, and find genomic evidence compatible with flea-mediated transmission causing bubonic plague.
- Maria A. Spyrou
- , Rezeda I. Tukhbatova
- & Johannes Krause
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| Open AccessPhylodynamic assessment of intervention strategies for the West African Ebola virus outbreak
During the last Ebola virus outbreak in West Africa, a large amount of viral genomic data was obtained. Here, Dellicour et al. use phylodynamic approaches to assess effect of intervention strategies such as border closures.
- Simon Dellicour
- , Guy Baele
- & Philippe Lemey
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Article
| Open AccessDephosphorylation of the HIV-1 restriction factor SAMHD1 is mediated by PP2A-B55α holoenzymes during mitotic exit
SAMHD1 is a critical restriction factor for HIV-1 and its antiviral activity is regulated by T592 phosphorylation. Here, Schott et al. show that the phosphatase PP2A-B55α dephosphorylates SAMHD1 during mitotic exit, rendering it antivirally active in G1 phase of primary CD4+ T cells.
- Kerstin Schott
- , Nina V. Fuchs
- & Renate König
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Article
| Open AccessA multi-modal MRI study of the central response to inflammation in rheumatoid arthritis
Many diseases, such as rheumatoid arthritis, are characterized by a chronic inflammatory state, but it is not clear whether or how this affects the brain. Here, the authors show that the severity of on-going inflammation predicts altered functional brain connectivity in people with rheumatoid arthritis.
- Andrew Schrepf
- , Chelsea M. Kaplan
- & Neil Basu
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Article
| Open AccessCREB controls cortical circuit plasticity and functional recovery after stroke
Increasing excitability in the peri-infarct area enhances motor recovery after stroke. Here the authors show that expressing CREB, a transcription factor known for its role in synaptic plasticity, or increasing activity of CREB-expressing cells near the stroke site improves recovery in an effect that is strong enough that it can be used to turn on and off motor recovery after stroke.
- L. Caracciolo
- , M. Marosi
- & S. T. Carmichael
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Article
| Open AccessCyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells
It is not clear whether and how incoming HIV-1 Tat accumulates in uninfected cells. Here, Chopard et al. show that, in uninfected cells, incoming Tat is palmitoylated on Cys31 by DHHC-20, which increases its affinity for PI(4,5)P2 and results in its accumulation at the plasma membrane.
- Christophe Chopard
- , Phuoc Bao Viet Tong
- & Bruno Beaumelle
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Article
| Open AccessMultiple communication mechanisms between sensor kinases are crucial for virulence in Pseudomonas aeruginosa
Bacteria respond to stresses using two-component systems consisting of sensor kinases (SKs) and response regulators. Here, Francis et al. reveal three specific interaction mechanisms between a pair of SKs that are important for regulation of virulence in the pathogen Pseudomonas aeruginosa.
- Vanessa I. Francis
- , Elaine M. Waters
- & Steven L. Porter
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Article
| Open AccessOxidation-specific epitopes restrain bone formation
Atherosclerosis and osteoporosis are epidemiologically associated, and oxidation specific epitopes (OSEs), which can be neutralized by innate antibodies, are pathogenic for both. Here, the authors show that mice expressing antibody fragments targeted to OSEs are protected from the bone loss induced by high-fat diet and have increased bone mass when fed a normal diet, and that levels of innate antibodies to OSEs decrease with ageing.
- Elena Ambrogini
- , Xuchu Que
- & Robert L. Jilka
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Article
| Open AccessCombined Rho-kinase inhibition and immunogenic cell death triggers and propagates immunity against cancer
Activation of an immune response is critical for the efficacy of cancer therapies. Here, the authors show that combination of ROCK inhibitor with chemotherapeutics that induce immunogenic cell death of cancer cells leads to increased dendritic cells’ maturation and synergistic CD8+ cytotoxic T cell priming and infiltration into the tumours, leading to suppressed tumour growth and improved overall survival in syngeneic and genetically engineered tumour models.
- Gi-Hoon Nam
- , Eun Jung Lee
- & In-San Kim
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Article
| Open AccessLaminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing
Laminins are important regulators of epidermal wound healing. Here, the authors show that laminins bind to multiple growth factors via their heparin-binding domains, and that incorporation of these domains into fibrin matrices increases growth factor retention, promoting wound healing in type 2 diabetic mouse models.
- Jun Ishihara
- , Ako Ishihara
- & Jeffrey A. Hubbell
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Article
| Open AccessArtificial strain of human prions created in vitro
Synthetic prions have previously been generated from recombinant rodent PrP. Here the authors generate synthetic human prions, by seeding human PrP with CJD prions, and characterize its infectivity in mice.
- Chae Kim
- , Xiangzhu Xiao
- & Jiri G. Safar
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Article
| Open AccessRecurrent rearrangements of FOS and FOSB define osteoblastoma
FOS has been linked to bone tumour pathogenesis, and viral homologue v-fos causes osteosarcoma in mice. Here, the authors report rearrangement of FOS and its paralogue FOSB in osteoblastoma and osteoid osteoma, revealing human bone tumours that are defined by mutations of FOS and FOSB.
- Matthew W. Fittall
- , William Mifsud
- & Sam Behjati
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Article
| Open AccessVariability in the location of high frequency oscillations during prolonged intracranial EEG recordings
High frequency oscillations (HFO) are a promising biomarker for identifying epileptogenic zones without the need to monitor spontaneous seizure episodes. Here the authors report that there is much variability in the location of HFOs offering a note of caution toward using HFO locations from short recordings as a guide for surgery.
- Stephen V. Gliske
- , Zachary T. Irwin
- & William C. Stacey
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Article
| Open AccessThe histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity
Steatosis is characterized by initial accumulation of lipids, followed by inflammation and ultimately fibrosis. Here the authors show that the histone demethylase Plant Homeodomain Finger 2 protects liver form steatosis progression by acting as a co-activator of ChREBP, thus, favouring lipid accumulation without inflammation.
- Julien Bricambert
- , Marie-Clotilde Alves-Guerra
- & Renaud Dentin
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Article
| Open AccessDominant-negative STAT5B mutations cause growth hormone insensitivity with short stature and mild immune dysregulation
Severe growth hormone insensitivity syndrome (GHIS) with immunodeficiency is caused by autosomal recessive mutations in STAT5B. Here the authors report heterozygous STAT5B mutations with dominant-negative effects, causing mild GHIS without immune defects.
- Jürgen Klammt
- , David Neumann
- & Vivian Hwa
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Article
| Open AccessThe hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages
The effects of iron on vacuole-resident Salmonella in macrophages are unclear. Here the authors show that the bacteria are not subject to nutritional inhibition by iron deprivation, but that iron depletion in the vacuole, via the hepcidin-ferroportin axis, inhibits the bactericidal effect of oxidative burst.
- Daejin Lim
- , Kwang Soo Kim
- & Hyon E. Choy
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Article
| Open AccessMale germ cells support long-term propagation of Zika virus
Zika virus (ZIKV) can persist for months in semen and sperm. Here, the authors show that germ cells, compared to other cell types in the reproductive tract, are most susceptible to ZIKV and produce high levels of progeny virus, which coincides with decreased expression of the interferon-stimulated gene Ifi44l.
- Christopher L. Robinson
- , Angie C. N. Chong
- & Shuibing Chen
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Article
| Open AccessFibrotic microtissue array to predict anti-fibrosis drug efficacy
A bottleneck in developing new anti-fibrosis therapies is the absence of suitable in vitro models that recapitulate key features of fibrogenesis. Here the authors develop a tissue-on-a-chip model of lung fibrosis and test the therapeutic efficacy of two recent FDA-approved drugs.
- Mohammadnabi Asmani
- , Sanjana Velumani
- & Ruogang Zhao
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Article
| Open AccessTestosterone is an endogenous regulator of BAFF and splenic B cell number
Testosterone deficiency is associated with autoimmunity and increased B cell numbers, but the underlying mechanism is unclear. Here the authors show that testosterone may modulate the production of B cell survival factor BAFF by fibroblastic reticular cells via regulation of splenic neurotransmitter levels.
- Anna S. Wilhelmson
- , Marta Lantero Rodriguez
- & Åsa Tivesten
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Article
| Open AccessLive-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
Diffuse intrinsic pontine gliomas exhibit a characteristic mutation of lysine 27 to methionine (K27M) in genes encoding histone H3.3. Here the authors show that the H3.3K27M mutation imposes a specific pattern of H3.3K27 methylation by altering the target search dynamics of PcG proteins.
- Roubina Tatavosian
- , Huy Nguyen Duc
- & Xiaojun Ren
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Article
| Open AccessWhole-genome sequencing reveals genomic signatures associated with the inflammatory microenvironments in Chinese NSCLC patients
The distinct genomic and epidemiological features of Chinese lung cancer patients suggest the presence of alternative causal mechanisms. Here, the authors present the genomic landscape of 149 Chinese NSCLC patients and reveal distinct mutational signatures associated with inflammatory microenvironments.
- Cheng Wang
- , Rong Yin
- & Hongbing Shen
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Article
| Open AccessProfiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity
Epithelial subpopulations are present in the human breast but how these differentiate or form is unclear. Here, the authors use single-cell RNA sequencing of primary human breast epithelial cells to define previously undescribed luminal, basal epithelial subpopulations and ZEB1-positive basal cells.
- Quy H. Nguyen
- , Nicholas Pervolarakis
- & Kai Kessenbrock
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Article
| Open AccessA20 critically controls microglia activation and inhibits inflammasome-dependent neuroinflammation
As resident macrophages of the brain, microglia are important for neuroinflammatory responses. This work shows that nuclear factor kappa B regulatory protein A20 is important for microglia activation and regulation during inflammation of the central nervous system.
- Sofie Voet
- , Conor Mc Guire
- & Geert van Loo
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Article
| Open AccessTargetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models
T- and NK-cell lymphomas (TCL) are a group of lymphoid malignancies characterized by poor prognosis, but the absence of appropriate pre-clinical models has hampered the development of effective therapies. Here the authors establish several pre-clinical models and identify vulnerabilities that could be further exploited to treat patients afflicted by these diseases.
- Samuel Y. Ng
- , Noriaki Yoshida
- & Raphael Koch
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Article
| Open AccessHypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota
Systemic chronic hypoxia is a feature of many diseases and may influence the communication between bone marrow and gut microbiota. Here, the authors show that chronic hypoxia predisposes bone marrow stem cells to premature senescence, which may be due to gut dysbiosis and gut microbiota-derived d-galactose accumulation.
- Junyue Xing
- , Yongquan Ying
- & Hao Zhang
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Article
| Open AccessIntron retention and nuclear loss of SFPQ are molecular hallmarks of ALS
Intron retention (IR) can increase protein diversity and function, and yet unregulated IR may be detrimental to cellular health. This study shows that aberrant IR occurs in ALS and finds nuclear loss of an RNA-binding protein called SFPQ as a new molecular hallmark in this devastating condition.
- Raphaelle Luisier
- , Giulia E. Tyzack
- & Rickie Patani
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Article
| Open AccessDeficiency of PRKD2 triggers hyperinsulinemia and metabolic disorders
Hyperinsulinemia can precede the development of insulin resistance. Here the authors identify a PKD2 mutation that leads to hyperinsulinemia and insulin resistance in Rhesus monkey and show that PKD2 deficiency promotes beta cell insulin secretion by activating L-type Ca2+ channels.
- Yao Xiao
- , Can Wang
- & Xiuqin Zhang
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Article
| Open AccessrbFOX1/MBNL1 competition for CCUG RNA repeats binding contributes to myotonic dystrophy type 1/type 2 differences
Myotonic dystrophy (DM) type 2 is a neuromuscular pathology caused by large expansions of CCTG repeats. Here the authors find that rbFOX1 RNA binding protein binds to CCUG RNA repeats and competes with MBNL1 for the binding to CCUG repeats, releasing MBNL1 from sequestration in DM2 muscle cells.
- Chantal Sellier
- , Estefanía Cerro-Herreros
- & Nicolas Charlet-Berguerand
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Article
| Open AccessHigh-throughput screening of prostate cancer risk loci by single nucleotide polymorphisms sequencing
Functional characterization of disease-causing variants at risk loci in cancer is challenging. Here, in prostate cancer the authors report a pipeline for high-throughput single-nucleotide polymorphisms sequencing (SNPs-seq) for large scale screening of functional SNPs at disease risk loci.
- Peng Zhang
- , Ji-Han Xia
- & Liang Wang
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Article
| Open AccessFactor XIIIA—expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
Lung squamous carcinomas (LUSC) are poorly molecularly characterized, but sub-populations show promising response to immune checkpoint inhibitors. Here, the authors identify a subset of LUSC characterized by infiltration of inflammatory monocytes, where metastasis is linked to Factor XIIIA promoting fibrin cross-linking.
- Alessandro Porrello
- , Patrick L. Leslie
- & Chad V. Pecot
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Article
| Open AccessEnhanced efficacy of combined temozolomide and bromodomain inhibitor therapy for gliomas using targeted nanoparticles
The blood-brain barrier often limits effective delivery of treatments for glioblastoma . In this study, the authors develop transferrin-functionalized nanoparticles able to traverse the intact blood-brain barrier and deliver combination temozolomide and bromodomain inhibitor therapy to glioma-bearing mice.
- Fred C. Lam
- , Stephen W. Morton
- & Paula T Hammond
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Article
| Open AccessMLL-fusion-driven leukemia requires SETD2 to safeguard genomic integrity
In leukemia, diverse fusion proteins involving the MLL gene can drive oncogenic activity. Here, the authors describe a dependency of MLL-leukemia cells on the methyltransferase SETD2 to maintain genomic integrity during leukemia initiation and maintenance.
- Anna Skucha
- , Jessica Ebner
- & Florian Grebien
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Article
| Open AccessCD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema
CD4+ T cells are critical for the development of lymphedema. Here the authors show how these cells contribute to lymphedema and identify that the sphingosine-1-phosphate receptor modulator FTY720 can prevent lymphedema in a mouse tail injury model by blocking the release of CD4+ T cells from the lymph nodes to the skin.
- Gabriela D. García Nores
- , Catherine L. Ly
- & Babak J. Mehrara
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Article
| Open AccessImmunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
In the context of increasing bacterial antibiotic-resistance, gene therapy that targets the immune system to clear infection is a major goal. Here the authors show a silicon based nanosystem that modulates the macrophage response in an in vivo model of Staphylococcal pneumonia.
- Byungji Kim
- , Hong-Bo Pang
- & Michael J. Sailor
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Article
| Open AccessA novel small molecule chaperone of rod opsin and its potential therapy for retinal degeneration
Mutations that lead to misfolding of rhodopsin can cause retinitis pigmentosa. Here, the authors carry out a high throughput screen to identify a small molecule chaperone of rod opsin, and show that it protects mouse models of retinitis pigmentosa from retinal degeneration.
- Yuanyuan Chen
- , Yu Chen
- & Krzysztof Palczewski
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Article
| Open AccessElucidating the genetic architecture of reproductive ageing in the Japanese population
The timing of female reproductive capacity is influenced by genetic and environmental factors. Here, in genome-wide association studies, the authors identify genetic loci for age at menarche and onset of menopause in Japanese women, and highlight differences with European populations.
- Momoko Horikoshi
- , Felix R. Day
- & John. R. B. Perry
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Article
| Open AccessLRRC8/VRAC anion channels enhance β-cell glucose sensing and insulin secretion
Insulin secretion by β-cells is stimulated by glucose and is dependent on the induction of β-cell membrane depolarization, mainly driven by the closure of KATP channels, which in turn promotes voltage-gated Ca2+ channel opening. Here the authors show that LRRC8 volume-regulated anion channels (VRACs) modulate glucose-stimulated calcium increase and insulin secretion.
- Till Stuhlmann
- , Rosa Planells-Cases
- & Thomas J. Jentsch
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Article
| Open AccessCalreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease
Inflammatory bowel disease (IBD) is initiated by integrins-mediated leukocyte adhesion to the activated colonic microvascular endothelium. Here, the authors show that inhibition of the calreticulin binding to integrin α subunits ameliorates the severity of IBD in animal models.
- Masayoshi Ohkuro
- , Jun-Dal Kim
- & Akiyoshi Fukamizu
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Article
| Open AccessA whole-genome sequence study identifies genetic risk factors for neuromyelitis optica
Neuromyelitis optica (NMO) is a rare autoimmune condition characterized by inflammation and demyelination of the optic nerve and the spinal cord. Here, Estrada et al. identify NMO susceptibility variants in the MHC region and find that autoantibody-positive NMO genetically overlaps with lupus.
- Karol Estrada
- , Christopher W. Whelan
- & Daniel G. MacArthur