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| Open AccessDiphtheria Toxin A-Resistant Cell Lines Enable Robust Production and Evaluation of DTA-Encoding Lentiviruses
- Margaret J. Lange
- , Terri D. Lyddon
- & Marc C. Johnson
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| Open AccessA synthetic male-specific sterilization system using the mammalian pro-apoptotic factor in a malaria vector mosquito
- Daisuke S. Yamamoto
- , Megumi Sumitani
- & Hirotomo Kato
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| Open AccessInduced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs
- Konrad Ocalewicz
- , Artur Gurgul
- & Stefan Dobosz
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| Open AccessAnephrogenic phenotype induced by SALL1 gene knockout in pigs
- Masahito Watanabe
- , Kazuaki Nakano
- & Hiroshi Nagashima
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| Open AccessComparative analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken
- T. K. Bhattacharya
- , Renu Shukla
- & S. K. Bhanja
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| Open AccessHigh-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins
- Peter Jan Vonk
- , Natalia Escobar
- & Robin A. Ohm
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| Open AccessTargeting mutations to the plastidial psbA gene of Chlamydomonas reinhardtii without direct positive selection
- Volha Shmidt
- , David Kaftan
- & Avihai Danon
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| Open AccessCRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons
- Carles Calatayud
- , Giulia Carola
- & Antonella Consiglio
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| Open AccessA novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products
- Ken Motohashi
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| Open AccessQuantification of allelic differential expression using a simple Fluorescence primer PCR-RFLP-based method
- Changzhi Zhao
- , Shengsong Xie
- & Xinyun Li
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| Open AccessConstruction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9
- You Kyeong Jeong
- , Jihyeon Yu
- & Sangsu Bae
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| Open AccessHighly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs
- Sachiko Okamoto
- , Yasunori Amaishi
- & Junichi Mineno
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| Open AccessA simple genotyping method to detect small CRISPR-Cas9 induced indels by agarose gel electrophoresis
- Debanjan Bhattacharya
- & Erwin G. Van Meir
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| Open AccessUnexpected genomic rearrangements at targeted loci associated with CRISPR/Cas9-mediated knock-in
- Amélie Rezza
- , Christelle Jacquet
- & Yacine Chérifi
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| Open AccessSuccessful Genetic Transfection of the Colonic Protistan Parasite Blastocystis for Reliable Expression of Ectopic Genes
- Feng-Jun Li
- , Anastasios D. Tsaousis
- & Kevin S. W. Tan
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| Open AccessA transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles
- Victoria N. Neckles
- , Mary C. Morton
- & David M. Feliciano
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| Open AccessZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on time requirement
- David Richter
- , Katharina Bayer
- & Stefan Schuster
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| Open AccessUnlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learning
- Aidan R. O’Brien
- , Laurence O. W. Wilson
- & Denis C. Bauer
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| Open AccessKnockout rat models mimicking human atherosclerosis created by Cpf1-mediated gene targeting
- Jong Geol Lee
- , Chang Hoon Ha
- & In-Jeoung Baek
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| Open AccessTargeted editing of the PSIP1 gene encoding LEDGF/p75 protects cells against HIV infection
- Yulia Lampi
- , Dominique Van Looveren
- & Rik Gijsbers
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| Open AccessA method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall
- Yuichi Furuhata
- , Ayako Sakai
- & Yoshio Kato
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| Open AccessHighly Efficient Transgenesis in Ferrets Using CRISPR/Cas9-Mediated Homology-Independent Insertion at the ROSA26 Locus
- Miao Yu
- , Xingshen Sun
- & John F. Engelhardt
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| Open AccessSimultaneous targeting of linked loci in mouse embryos using base editing
- Hye Kyung Lee
- , Michaela Willi
- & Lothar Hennighausen
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| Open AccessRobust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system
- Sho Yoshimatsu
- , Junko Okahara
- & Hideyuki Okano
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| Open AccessIntelectin 3 is dispensable for resistance against a mycobacterial infection in zebrafish (Danio rerio)
- Markus J. T. Ojanen
- , Meri I. E. Uusi-Mäkelä
- & Mika Rämet
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| Open AccessRapid diagnosis of Mycoplasma pneumonia infection by denaturation bubble-mediated strand exchange amplification: comparison with LAMP and real-time PCR
- Wenqiang Shi
- , Manman Wei
- & Chao Shi
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| Open AccessLiposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems
- Sally Adams
- , Prachi Pathak
- & Andre Pires-daSilva
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| Open AccessGeneration of H7N9-specific human polyclonal antibodies from a transchromosomic goat (caprine) system
- Hua Wu
- , Zhiqiang Fan
- & Eddie J. Sullivan
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| Open AccessGeneration of a highly efficient and tissue-specific tryptophan hydroxylase 1 knockout mouse model
- Hyeongseok Kim
- , Yeong Gi Kim
- & Hail Kim
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| Open AccessA novel cloning strategy for one-step assembly of multiplex CRISPR vectors
- Marc Zuckermann
- , Mario Hlevnjak
- & Jan Gronych
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| Open AccessMapping of a responsible region for sex reversal upstream of Sox9 by production of mice with serial deletion in a genomic locus
- Yuya Ogawa
- , Miho Terao
- & Shuji Takada
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| Open AccessGenetic engineering a large animal model of human hypophosphatasia in sheep
- Diarra K. Williams
- , Carlos Pinzón
- & Dana Gaddy
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| Open AccessEffects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish
- Ahmed Elaswad
- , Karim Khalil
- & Rex Dunham
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| Open AccessQuantitative evaluation of incomplete preweaning lethality in mice by using the CRISPR/Cas9 system
- Takumi Nakamura
- , Kazuo Nakajima
- & Tadafumi Kato
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| Open AccessHigh fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells
- Alewo Idoko-Akoh
- , Lorna Taylor
- & Michael J. McGrew
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| Open AccessDual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells
- Julia Baaske
- , Patrick Gonschorek
- & Matias D. Zurbriggen
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| Open AccessEngineering functional BMP-2 expressing teratoma-derived fibroblasts for enhancing osteogenesis
- Yoon Young Go
- , Ji Yeon Mun
- & Jae-Jun Song
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| Open AccessA Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
- Kaushalya Kulathunga
- , Michito Hamada
- & Yoshihiro Miwa
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| Open AccessA single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
- Madina Karimova
- , Oliver Baker
- & Konstantinos Anastassiadis
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| Open AccessTransgene-free genome editing in marine algae by bacterial conjugation – comparison with biolistic CRISPR/Cas9 transformation
- A. K. Sharma
- , M. Nymark
- & P. Winge
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| Open AccessEstablishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato
- Hiroaki Kusano
- , Mariko Ohnuma
- & Hiroaki Shimada
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| Open AccessA tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
- Tomoya Shiraki
- & Koichi Kawakami
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| Open AccessA novel glycosylated anti-CD20 monoclonal antibody from transgenic cattle
- Ran Zhang
- , Chenjun Tang
- & Ning Li
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| Open AccessRegulating exopolysaccharide gene wcaF allows control of Escherichia coli biofilm formation
- Jingyun Zhang
- & Chueh Loo Poh
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| Open AccessCRISPR/Cas9-Mediated Generation of Guangxi Bama Minipigs Harboring Three Mutations in α-Synuclein Causing Parkinson’s Disease
- Xiang-Xing Zhu
- , Yi-Zhi Zhong
- & Sheng-Sheng Lu
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| Open Accessi-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats
- Shuji Takabayashi
- , Takuya Aoshima
- & Masahiro Sato
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| Open AccessA Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDV
- Edris Shirvani
- , Anandan Paldurai
- & Siba K. Samal
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| Open AccessHigh doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion
- Tsuyoshi Momose
- , Anne De Cian
- & Jean-Paul Concordet
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| Open AccessIn vivo targeted single-nucleotide editing in zebrafish
- Shingo Tanaka
- , Shin Yoshioka
- & Shingo Maegawa