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Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
To identify genes affecting traits of interest in E. coli, Warner et al. describe a method to rapidly create and assay rationally mutated versions of every gene. The approach is applied to several traits, including tolerance to cellulosic hydrolysate, a biofuel precursor.
- Joseph R Warner
- , Philippa J Reeder
- & Ryan T Gill
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A mouse knockout library for secreted and transmembrane proteins
Tang et al. present the first large-scale, gene-specific library of knockout mice. They disrupt 472 genes encoding secreted or transmembrane proteins and report the results of a comprehensive phenotypic analysis.
- Tracy Tang
- , Li Li
- & Frederic J de Sauvage
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Assessing therapeutic responses in Kras mutant cancers using genetically engineered mouse models
Genetically engineered mouse models of cancer simulate the spontaneous development of tumors in their native tissue environment. Singh et al. establish their ability to predict the efficacy of different treatment regimens by comparing clinical trial results to equivalent experiments in mutated KRAS-driven mouse models of pancreatic and lung cancer.
- Mallika Singh
- , Anthony Lima
- & Leisa Johnson