Featured
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Editorial |
Humans 2.0
Of the germline engineering approaches, mitochondrial replacement, rather than gene editing, is poised to have the greatest impact on our lives.
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News Feature |
Better beings?
As the technology to create genetically modified babies moves closer to practice, what questions should we ask before such procedures are contemplated? Amber Dance investigates.
- Amber Dance
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Editorial |
Time for the data to speak
Retraction of a study claiming gene editing via an Argonaute enzyme illustrates the importance of post-publication peer review in the age of 24/7 media.
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Correspondence |
Rules of the road for insect gene drive research and testing
- Zach Adelman
- , Omar Akbari
- & Chris Wozniak
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Correspondence |
FDA is the wrong agency to regulate genetically engineered animals
- John J Cohrssen
- & Henry I Miller
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News Feature |
Hanging on a thread
Several companies are now banking on bioengineered silk to create a new generation of textiles and materials...and a necktie. Laura DeFrancesco investigates.
- Laura DeFrancesco
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News & Views |
Base editing on the rise
Research on base editing leads to new enzymes, genome-wide specificity measurements, and edited plants and animals.
- Andrew May
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Correspondence |
With all due respect to Maholo, lab automation isn't anthropomorphic
- David W McClymont
- & Paul S Freemont
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Letter |
Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
An antinutritional compound in Brassica seed meal is reduced by knocking out genes encoding transporter proteins.
- Hussam Hassan Nour-Eldin
- , Svend Roesen Madsen
- & Barbara Ann Halkier
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Brief Communication |
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Single DNA base pairs are edited in wheat, rice and maize using a Cas9 nickase fusion protein.
- Yuan Zong
- , Yanpeng Wang
- & Caixia Gao
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Letter |
Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions
Genome editing of single bases is made more versatile and accurate with new fusions of mutated Cas9 and cytidine deaminase domains.
- Y Bill Kim
- , Alexis C Komor
- & David R Liu
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Article |
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
An approach called CREATE enables multiplex genome engineering, protein engineering and mapping of mutations in bacterial and yeast cells.
- Andrew D Garst
- , Marcelo C Bassalo
- & Ryan T Gill
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News |
First-in-human CRISPR trial
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News & Views |
Automated design of DNA origami
A top-down computational method enables fabrication of DNA objects in any shape.
- Veikko Linko
- & Mauri A Kostiainen
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Patents |
Recent patents in gene editing
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Correspondence |
Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins
- Junho K Hur
- , Kyoungmi Kim
- & Jin-Soo Kim
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Correspondence |
Generation of knockout mice by Cpf1-mediated gene targeting
- Yongsub Kim
- , Seung-A Cheong
- & Young Hoon Sung
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Letter |
Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells
On-target and off-target specificities of Cfp1 are assessed and compared with SpCas9 in human cells.
- Daesik Kim
- , Jungeun Kim
- & Jin-Soo Kim
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Letter |
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
A small-molecule-inducible Cas9 variant with very low background activity is identified by screening for sites that can tolerate domain insertions.
- Benjamin L Oakes
- , Dana C Nadler
- & David F Savage
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Brief Communication |
CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes
CRISPR knockout screens outperform shRNA and CRISPR-interference screens in a side-by-side comparison.
- Bastiaan Evers
- , Katarzyna Jastrzebski
- & Rene Bernards
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News |
GM salmon shut out of US
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Correspondence |
Marketplace response to GM animal products
- Damien Mather
- , Rasmus Vikan
- & John Knight
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Letter |
Targeted gene addition in human CD34+ hematopoietic cells for correction of X-linked chronic granulomatous disease
Targeted genetic correction of hematopoietic stem cells is applied to X-linked chronic granulomatous disease.
- Suk See De Ravin
- , Andreas Reik
- & Harry L Malech
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News |
Bayer joins CRISPR
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News & Views |
Can CRISPR-Cas9 gene drives curb malaria?
Gene drives in mosquitoes to reduce the spread of malaria move closer, though technical and regulatory hurdles remain.
- Luke Alphey
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Article |
High-throughput mapping of regulatory DNA
Cis-regulatory genomic regions necessary for gene expression are revealed by a high-throughput CRISPR-Cas9–based assay.
- Nisha Rajagopal
- , Sharanya Srinivasan
- & Richard I Sherwood
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Letter |
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA
The efficiency of homology-directed genome editing with CRISPR-Cas9 is boosted through improved design of donor DNA.
- Christopher D Richardson
- , Graham J Ray
- & Jacob E Corn
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Article |
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms.
- John G Doench
- , Nicolo Fusi
- & David E Root
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Editorial |
Xenotransplantation 2.0
Will targeted immunosuppressants and new tools in genome engineering be enough to finally give xenopigs wings?
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Correspondence |
Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus
- Kristin M Whitworth
- , Raymond R R Rowland
- & Randall S Prather
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Editorial |
20 years of bio-lox
Here's hoping that a proposed shakeup of US regulations will mean that new biotech products avoid AquaAdvantage salmon's two-decade upstream struggle to regulatory approval.
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News |
Sexed-up beer
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