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| Open AccessActive thrombin produced by the intestinal epithelium controls mucosal biofilms
The roles played by thrombin in the human intestinal mucosa are unclear. Here, the authors show that the commensal microbiota modulates epithelial production of active thrombin, which controls biofilm growth and contributes to protection of the mucosa from bacterial invasion.
- Jean-Paul Motta
- , Alexandre Denadai-Souza
- & Nathalie Vergnolle
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Article
| Open AccessGenomic signatures reveal DNA damage response deficiency in colorectal cancer brain metastases
The development of brain metastases is a lethal yet poorly understood event in the evolution of many cancers. Here, the authors perform whole-genome and whole-exome sequencing on matched normal, primary and metastatic tissue samples to explore the genomic features of brain metastases in colorectal cancer.
- Jing Sun
- , Cheng Wang
- & Yanhong Gu
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Article
| Open AccessDefective HNF4alpha-dependent gene expression as a driver of hepatocellular failure in alcoholic hepatitis
Alcoholic hepatitis, a common cause of liver failure, lacks effective treatment. Here, the authors show altered hepatic HNF4a isoform expression and hypermethylation of its target genes in patients. HNF4a dysregulation is improved in vitro by TGFb or PPARg modulation suggesting potential therapeutic avenues.
- Josepmaria Argemi
- , Maria U. Latasa
- & Ramon Bataller
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Article
| Open AccessActivation of STAT3 signaling is mediated by TFF1 silencing in gastric neoplasia
Trefoil factor 1 (TFF1) is a protein secreted by the gastric mucosa that protects against gastric tumourigenesis. Here, the authors show that TFF1 inhibits the oncogenic inflammatory response and IL-6-mediated STAT3 activation by interfering with the binding of IL6 to its receptor IL6Rα.
- Mohammed Soutto
- , Zheng Chen
- & Wael El-Rifai
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Article
| Open AccessConserved transcriptomic profile between mouse and human colitis allows unsupervised patient stratification
Clinical and molecular heterogeneity of ulcerative colitis presents unresolved challenges to identify predictive biomarkers of response to therapies. Here, the authors combine mouse colitis time course with patient biopsy transcriptomes, achieving unsupervised clustering of UC patients correlating with therapeutic outcomes in independent data sets.
- Paulo Czarnewski
- , Sara M. Parigi
- & Eduardo J. Villablanca
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Article
| Open AccessMass cytometry reveals systemic and local immune signatures that distinguish inflammatory bowel diseases
Distinguishing clinical subtypes of IBD is critical for optimal treatments, outcome prediction, and better understanding of disease pathogenesis. Here the authors phenotype blood and intestinal immune cells by mass cytometry and identify signatures associated with distinct disease states.
- Samuel J. S. Rubin
- , Lawrence Bai
- & Aida Habtezion
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Article
| Open AccessTargeting EZH2 histone methyltransferase activity alleviates experimental intestinal inflammation
EZH2-mediated methylatation of histone 3 is essential for immune regulation. Here, the authors show that EZH2 inhibitors attenuate experimental colitis in mice by promoting the development of myeloid-derived suppressor cells, and delay the onset of colitis-associated cancer.
- Jie Zhou
- , Shuo Huang
- & Bo Zhu
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Article
| Open AccessSuppression of a broad spectrum of liver autoimmune pathologies by single peptide-MHC-based nanomedicines
Immune response against tissue-specific antigens is a hallmark of autoimmunity. Here the authors show that a single autoantigen-based nanomedicine can ameliorate pathology in a broad range of liver autoimmunity models without impairing host defenses, suggesting organ-wide tolerization.
- Channakeshava Sokke Umeshappa
- , Santiswarup Singha
- & Pere Santamaria
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Article
| Open AccessA clinically translatable hyperspectral endoscopy (HySE) system for imaging the gastrointestinal tract
Hyperspectral imaging (HSI) enables recording both morphological and biochemical information, but image acquisition time and geometric distortions limit its clinical applicability. Here the authors overcome these challenges with an endoscope combining HSI and white light to correct for image distortion during freehand operation.
- Jonghee Yoon
- , James Joseph
- & Sarah E. Bohndiek
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Article
| Open AccessThe H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis
The histone variant, H2A.Z is known to regulate gene expression and cell proliferation. Here the authors show that H2A.Z has a central role in the control of intestinal epithelial homeostasis in mice, by preventing terminal differentiation of intestinal progenitors.
- Jérémie Rispal
- , Lucie Baron
- & Fabrice Escaffit
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Article
| Open AccessEarly life stress disrupts intestinal homeostasis via NGF-TrkA signaling
Early life stress has been associated with the occurrence of gastrointestinal diseases later in life, but underlying mechanisms remain poorly understood. Here, Wong et al. show that early life stress leads to expansion of intestinal stem cells and their differentiation into serotonin-producing enterochromaffin cells through crosstalk between NGF and Wnt signalling pathways.
- Hoi Leong Xavier Wong
- , Hong-yan Qin
- & Zhao-xiang Bian
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Article
| Open AccessHepatocyte-specific loss of GPS2 in mice reduces non-alcoholic steatohepatitis via activation of PPARα
Dysregulation of PPARα dependent fatty acid oxidation promotes hepatic steatosis. Here the authors show that GPS2 inhibits PPARα activity and that ablation of GPS2 ameliorates hepatic steatosis in mice.
- Ning Liang
- , Anastasius Damdimopoulos
- & Rongrong Fan
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Article
| Open AccessA gut microbiome signature for cirrhosis due to nonalcoholic fatty liver disease
Development of cirrhosis in individuals with non-alcoholic fatty liver disease can predict mortality. Here the authors used a unique twin and family cohort to identify a gut microbiome-derived 16sRNA signature that can detect cirrhosis in individuals with non-alcoholic fatty liver disease.
- Cyrielle Caussy
- , Anupriya Tripathi
- & Rohit Loomba
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Article
| Open AccessDuodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2
Gluten triggers celiac disease in genetically predisposed individuals, but additional unknown mechanisms are required. Here, the authors show that proteases from Pseudomonas aeruginosa can modulate inflammatory pathways that are relevant to the development of food sensitivities, independently of the trigger antigen.
- Alberto Caminero
- , Justin L. McCarville
- & Elena F. Verdu
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Article
| Open AccessTissue-resident Eomes+ NK cells are the major innate lymphoid cell population in human infant intestine
Innate lymphoid cells (ILC), including natural killer (NK) cells, are important innate immune regulators. Here the authors show that, in human infant intestines, CD103+Eomes+ NK cells are the predominant ILC population, but are replaced gradually by Eomes+ T cells, while NKp44+ NK cells persist in adult intestines.
- Adrian F. Sagebiel
- , Fenja Steinert
- & Madeleine J. Bunders
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Article
| Open AccessHepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism
Endoplasmic reticulum (ER) stress has been proposed to play a role in metabolic diseases. Here, Sasako and colleagues identify stromal cell-derived factor 2 like 1 (Sdf2l1) as a regulator of the ER stress response to feeding in the liver, and suggest that its downregulation may promote diabetes and hepatic steatosis in humans.
- Takayoshi Sasako
- , Mitsuru Ohsugi
- & Kohjiro Ueki
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Article
| Open AccessThe p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer
MicroRNA miR-500a-5p has been linked to oncogenesis but its role in colorectal cancer (CRC) is largely unknown. Here, the authors investigate the role of miR-500a-5p in CRC in vitro and in vivo models and find that miR-500a-5p acts as a tumour suppressor in CRC by targeting the p300/YY1/HDAC2 axis.
- Weimei Tang
- , Weijie Zhou
- & Jide Wang
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Article
| Open AccessATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens
The generation of protective secretory IgA is a desired outcome of oral vaccination. Here, the authors show that the depletion of intestinal ATP significantly improves the production and response of high-affinity IgA against both live and inactivated oral vaccines.
- Michele Proietti
- , Lisa Perruzza
- & Fabio Grassi
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Article
| Open AccessEnhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway
Urolithins are microbial metabolites derived from food polyphenols. Here, Singh et al. show that urolithin A and a synthetic analogue enhance gut barrier function via Nrf2-dependent pathways and mitigate inflammation and colitis in mice, highlighting a potential application for inflammatory bowel diseases.
- Rajbir Singh
- , Sandeep Chandrashekharappa
- & Venkatakrishna R. Jala
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Article
| Open AccessUlcerative colitis mucosal transcriptomes reveal mitochondriopathy and personalized mechanisms underlying disease severity and treatment response
The severity of ulcerative colitis, and response to treatment, is highly variable. Here, the authors examine rectal gene expression signatures and faecal microbiomes of children and adults with the disease and provide new insights in to pathogenesis.
- Yael Haberman
- , Rebekah Karns
- & Lee A. Denson
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Article
| Open AccessMolecular and functional heterogeneity of IL-10-producing CD4+ T cells
Tr1 cells are considered an immunosuppressive CD4 T cell population producing IL-10. Here the authors show that IL-10 is insufficient for Tr1 immunosuppression, define surface markers and transcriptional program of the immunosuppressive subset within Tr1, and reveal its deficiency in patients with IBD.
- Leonie Brockmann
- , Shiwa Soukou
- & Samuel Huber
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Article
| Open AccessStructural assembly of the megadalton-sized receptor for intestinal vitamin B12 uptake and kidney protein reabsorption
Cubilin and the transmembrane protein amnionless (AMN) form the endocytic receptor cubam that is essential for intestinal vitamin B12 uptake. Here the authors present the 2.3 Å crystal structure of AMN in complex with the amino-terminal region of cubilin and discuss cubam architecture and disease causing mutations.
- Casper Larsen
- , Anders Etzerodt
- & Christian Brix Folsted Andersen
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Article
| Open AccessAn inflammatory-CCRK circuitry drives mTORC1-dependent metabolic and immunosuppressive reprogramming in obesity-associated hepatocellular carcinoma
Obesity increases the risk of hepatocellular carcinoma (HCC) especially in men. Here the authors find a potential mechanistic explanation by showing that, in mice, obesity-induced STAT3 cooperates with the androgen receptor to activate the mTORC pathway through up regulation of CCRK, resulting in hepatic steatosis worsening and HCC development via metabolic and immune reprogramming.
- Hanyong Sun
- , Weiqin Yang
- & Alfred S. L. Cheng
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Article
| Open AccessTherapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells
Faecal microbiota transplantation (FMT) is becoming a therapeutic option in several gastrointestinal disorders. Here, Burrello et al. study the immunological mechanisms by which FMT reduces colonic inflammation and initiates the restoration of intestinal homeostasis in a mouse model of colitis.
- Claudia Burrello
- , Federica Garavaglia
- & Federica Facciotti
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Article
| Open AccessGenome-wide association meta-analysis yields 20 loci associated with gallstone disease
Genome-wide association studies have so far identified eight risk loci for gallstone disease. Here, the authors perform meta-analysis in cohorts from Iceland and the UK which reveals further 21 common and low-frequency risk variants that highlight the role of bile acid homeostasis in gallstone disease.
- Egil Ferkingstad
- , Asmundur Oddsson
- & Kari Stefansson
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Article
| Open AccessROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-β signalling
SLIT-ROBO alterations arise in pancreatic ductal adenocarcinoma (PDAC), but their role in the pancreas is unclear. Here, the authors use mouse models to show that loss of epithelial Robo2 activates the neighbouring stroma via TGF-β signalling; findings are relevant to PDAC patients, where ROBO expression correlates with survival outcomes.
- Andreia V. Pinho
- , Mathias Van Bulck
- & Ilse Rooman
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Article
| Open AccessA preclinical model of chronic pancreatitis driven by trypsinogen autoactivation
Inflammatory diseases of the pancreas are currently untreatable and lack a pre-clinical model that recapitulates the hallmarks of the human pathology. Here, the authors generate a knock-in mouse strain with increased tripsinogen autoactivation and show that it develops spontaneous pancreatic pathology with some key features of human pancreatitis.
- Andrea Geisz
- & Miklós Sahin-Tóth
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Article
| Open AccessHuman milk oligosaccharides, milk microbiome and infant gut microbiome modulate neonatal rotavirus infection
Neonatal rotavirus infections are associated with gastrointestinal symptoms in some settings, but the role of host factors in clinical presentation is unclear. Here, Ramani et al. show that human milk oligosaccharides and microbiome are associated with symptomatic infection with neonatal strain G10P[11].
- Sasirekha Ramani
- , Christopher J. Stewart
- & Mary K. Estes
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Article
| Open AccessSerotonin signals through a gut-liver axis to regulate hepatic steatosis
No effective pharmacological treatments exist for nonalcoholic fatty liver disease (NAFLD). Here, the authors show that serotonin concentration in the portal blood is increased in nine human subjects and in mice fed a high-fat diet, and that local serotonin signaling ablation, either genetically or with an antagonist, prevents hepatic steatosis in mice.
- Wonsuk Choi
- , Jun Namkung
- & Hail Kim
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Article
| Open AccessPerforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis
CD8 T cells can protect the liver from viral infection, but can also result in severe liver damage and organ failure. Here, the authors develop a mouse model reflecting fulminant CD8 T cell mediated viral hepatitis, which occurs in a perforin-dependent manner that is protected by the use of perforin inhibitors.
- M. Welz
- , S. Eickhoff
- & W. Kastenmüller
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Article
| Open AccessΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis
Bacteriophages of the crAssphage family have not yet been isolated, despite being highly abundant in the human gut. Here, Shkoporov et al. isolate in pure culture one of these viruses and show that it infects the human gut symbiont Bacteroides intestinalis.
- Andrey N. Shkoporov
- , Ekaterina V. Khokhlova
- & Colin Hill
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Article
| Open AccessProteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN
Ubiquitin-specific protease 14 (USP14) is a proteasome-associated deubiquitinating enzyme with known roles in physiology and disease. Here the authors show that fatty acid synthase (FASN) is a substrate of USP14, and that by stabilizing FASN, it plays a role in hepatosteatosis.
- Bin Liu
- , Shangwen Jiang
- & Minjia Tan
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Article
| Open AccessA low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults
Gluten-free diets are increasingly common in the general population. Here, the authors report the results of a randomised cross-over trial involving middle-aged, healthy Danish adults, showing evidence that a low-gluten diet leads to gut microbiome changes, possibly due to variations in dietary fibres.
- Lea B. S. Hansen
- , Henrik M. Roager
- & Oluf Pedersen
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Article
| Open AccessThe gut microbiota in infants of obese mothers increases inflammation and susceptibility to NAFLD
Infants born to obese mothers have altered microbiome and increased risk of obesity and NAFLD. Here the authors establish causality by showing that maternal obesity-shaped infant gut microbiome induces macrophage dysfunction, inflammation, and diet-induced metabolic disease in germ-free mice.
- Taylor K. Soderborg
- , Sarah E. Clark
- & Jacob E. Friedman
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Article
| Open AccessSingle cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations
The development of single cell RNA sequencing technologies has been instrumental in advancing our understanding of tissue biology. Here, MacParland et al. performed single cell RNA sequencing of human liver samples, and identify distinct populations of intrahepatic macrophages that may play specific roles in liver disease.
- Sonya A. MacParland
- , Jeff C. Liu
- & Ian D. McGilvray
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Article
| Open AccessREV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis
REV-ERBα is a nuclear receptor that links the circadian pathways with those of metabolism. Here the authors show REV-ERBα is also involved with linking the circadian system with the inflammatory pathways of an experimental model of colitis through regulation of the NF-κB/NLRP3 axis.
- Shuai Wang
- , Yanke Lin
- & Baojian Wu
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Article
| Open AccessLRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival
Inflammatory bowel disease is characterised by epithelial dysfunction. Here the authors show that loss of the nuclear receptor LRH-1 leads to epithelial disruption by altering Notch signaling in mouse intestinal organoids, and that LRH-1 overexpression ameliorates immune-mediated colitis in a mouse model.
- James R. Bayrer
- , Hongtao Wang
- & Holly A. Ingraham
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Article
| Open AccessMeta-analysis of effects of exclusive breastfeeding on infant gut microbiota across populations
Studies on the effects of breastfeeding on the infant gut microbiota have provided inconsistent results. Here, Ho et al. perform a meta-analysis of seven studies across different populations, supporting that exclusive breastfeeding is associated with short-term and long-term alterations in the infant gut microbiota.
- Nhan T. Ho
- , Fan Li
- & Louise Kuhn
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Article
| Open AccessEpigenomic map of human liver reveals principles of zonated morphogenic and metabolic control
Spatial mapping of genomic programs in tissue cells is an important step in the understanding of organ function and disease. Here, the authors provide a spatially resolved epigenomic and transcriptomic map of human liver and show porto-central gradients in metabolic and morphogen networks and transcription factor binding sites as a basis to better understand liver regeneration and function.
- Mario Brosch
- , Kathrin Kattler
- & Jochen Hampe
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Article
| Open AccessAlpha kinase 1 controls intestinal inflammation by suppressing the IL-12/Th1 axis
The Hiccs locus has been associated with susceptibility to colitis in mice. Here the authors identify a Hiccs locus gene encoding Alpha kinase 1 as a potent regulator of intestinal inflammation via modulation of the IL-12/Th1 axis.
- Grigory Ryzhakov
- , Nathaniel R. West
- & Fiona Powrie
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Article
| Open AccessGut fungal dysbiosis correlates with reduced efficacy of fecal microbiota transplantation in Clostridium difficile infection
Fecal microbiota transplantation (FMT) is effective in treating recurrent Clostridium difficile infection (CDI). Here, the authors show that the composition of the gut fungal microbiota of donors and recipients, and especially the abundance of Candida, correlates with FMT outcome in CDI patients.
- Tao Zuo
- , Sunny H. Wong
- & Siew C. Ng
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Article
| Open AccessMicrobiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis
T cells play a critical role in intestinal homeostasis, with increasing evidence suggesting a role for the microbiome metabolome in modulating this response. Here the authors show short-chain fatty acids promote IL-10 production in Th1 cells.
- Mingming Sun
- , Wei Wu
- & Yingzi Cong
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Review Article
| Open AccessMicrobial tryptophan catabolites in health and disease
Gut microbial metabolites are known to impact many physiological processes of the host and play a critical role in immune-homeostasis. Here the authors review our current understanding and appreciation of the importance of microbially derived tryptophan catabolites during both health and disease.
- Henrik M. Roager
- & Tine R. Licht
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Review Article
| Open AccessResolution of chronic inflammatory disease: universal and tissue-specific concepts
Inflammation is a component of many chronic inflammatory diseases and yet it is understudied in medicine. Here, the authors review novel insights in to inflammation and how impairment of its resolution can lead to diseases.
- Georg Schett
- & Markus F. Neurath
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Article
| Open AccessManic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells
In intestinal adenomas carrying active β-catenin, Jagged1 (Jag1) ligand is responsible for Notch1 activation. Here, the authors show the reliance of Notch on Jag1 in cancer, and investigate how Jag1–Notch1 signaling interference may provide therapeutic benefits in some colorectal cancer patients
- Erika López-Arribillaga
- , Verónica Rodilla
- & LLuís Espinosa
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Article
| Open AccessLong noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis
The LXR-SREBP1c pathway promotes hepatic lipogenesis that is deregualted in fatty liver disease. Here the authors show that the long noncoding RNA Blnc1 contributes to the development of obesity-driven steatosis by enabling SREBP1c trascriptional activity in response to LXR activation.
- Xu-Yun Zhao
- , Xuelian Xiong
- & Jiandie D. Lin
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Article
| Open AccessMetaproteomics reveals associations between microbiome and intestinal extracellular vesicle proteins in pediatric inflammatory bowel disease
Gut microbial dysbiosis has been implicated in the pathogenesis of inflammatory bowel disease. Here, the authors examine host-microbiota protein interactions that occur in inflammatory bowel disease; they show an upregulation in proteins related to antimicrobial activities, and alterations in intestinal extracellular vesicles that are associated with aberrant microbiota-interactions.
- Xu Zhang
- , Shelley A. Deeke
- & Daniel Figeys
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Article
| Open AccessInsulin/Snail1 axis ameliorates fatty liver disease by epigenetically suppressing lipogenesis
Insulin promotes lipogenesis but, on the other hand, insulin resistance is associated with increased lipogenesis in the liver. Here the authors show that Snail1 is upregulated by insulin and inhibits lipogenesis by repressing Fasn expression but insulin-mediated Snail1 upregulation is impaired during obesity and insulin resistance.
- Yan Liu
- , Lin Jiang
- & Liangyou Rui
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Article
| Open AccessPostprandial FGF19-induced phosphorylation by Src is critical for FXR function in bile acid homeostasis
FXR plays an important role in bile acid homeostasis by transcriptionally modulating several enterohepatic genes, including intestinal FGF19, that repress hepatic bile acid synthesis. Here the authors show that postprandial FGF19 regulates FXR transcriptional activity via its action on the tyrosine kinase Src, which phosphorylates FXR.
- Sangwon Byun
- , Dong-Hyun Kim
- & Jongsook Kim Kemper