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High-throughput Oligopaint screen identifies druggable 3D genome regulators
High-throughput DNA or RNA labelling with optimized Oligopaints (HiDRO) reveals more than 300 factors that influence genome folding during interphase, including 43 genes that were validated as either increasing or decreasing interactions between topologically associating domains.
- Daniel S. Park
- , Son C. Nguyen
- & Eric F. Joyce
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Locomotion activates PKA through dopamine and adenosine in striatal neurons
Dopamine and adenosine act together in the striatum to regulate protein kinase A activity, which in turn coordinates animal locomotion.
- Lei Ma
- , Julian Day-Cooney
- & Haining Zhong
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Article
| Open AccessSpatial profiling of chromatin accessibility in mouse and human tissues
Spatial-ATAC-seq—spatially resolved chromatin accessibility profiling of tissue sections using next-generation sequencing—delineated tissue-region-specific epigenetic landscapes in mouse embryos and identified gene regulators involved in the development of the central nervous system and the lymphoid tissue.
- Yanxiang Deng
- , Marek Bartosovic
- & Rong Fan
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Multiview confocal super-resolution microscopy
A combination of multiview imaging, structured illumination, reconstruction algorithms and deep-learning predictions realizes spatial- and temporal-resolution improvements in fluorescence microscopy to produce super-resolution images from diffraction-limited input images.
- Yicong Wu
- , Xiaofei Han
- & Hari Shroff
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Article
| Open AccessSpatially resolved cell atlas of the mouse primary motor cortex by MERFISH
As part of the BICCN consortium, the authors used a single-cell transcriptomic imaging method to produce a highly defined atlas of cell types across the mouse primary motor cortex.
- Meng Zhang
- , Stephen W. Eichhorn
- & Xiaowei Zhuang
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Article |
Distinct fission signatures predict mitochondrial degradation or biogenesis
Mitochondrial fission at the organelle periphery generates small daughter mitochondria that are removed by mitophagy whereas fission at the midzone leads to proliferation.
- Tatjana Kleele
- , Timo Rey
- & Suliana Manley
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Article |
Cell-type-specific asynchronous modulation of PKA by dopamine in learning
The net PKA activities in each class of spiny projection neuron in the nucleus accumbens of the mouse are dichotomously modulated by asynchronous positive and negative dopamine signals during different phases of learning.
- Suk Joon Lee
- , Bart Lodder
- & Bernardo L. Sabatini
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Article |
Hidden neural states underlie canary song syntax
Neurons in the canary premotor cortex homologue encode past song phrases and transitions, carrying information relevant to future choice of phrases as ‘hidden states’ during song.
- Yarden Cohen
- , Jun Shen
- & Timothy J. Gardner
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Article |
Neural signatures of sleep in zebrafish
Fluorescence-based polysomnography in zebrafish reveals two major sleep signatures that share features with those of amniotes, which suggests that common neural sleep signatures emerged in the vertebrate brain over 450 million years ago.
- Louis C. Leung
- , Gordon X. Wang
- & Philippe Mourrain
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Self-organization and symmetry breaking in intestinal organoid development
Single-cell-based imaging and sequencing approaches are used to characterize organoid development and the intestinal regeneration process, which is driven by transient activation of YAP1.
- Denise Serra
- , Urs Mayr
- & Prisca Liberali
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Letter |
Applying systems-level spectral imaging and analysis to reveal the organelle interactome
Using confocal and lattice light sheet microscopy, the authors perform systems-level analysis of the organelle interactome in live cells, allowing them to visualize the frequency and locality of up to five-way interactions between different organelles.
- Alex M. Valm
- , Sarah Cohen
- & Jennifer Lippincott-Schwartz
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Letter |
TAM receptors regulate multiple features of microglial physiology
Microglial phagocytosis is required for neurogenic niche maintenance and response to injury; the TAM kinases Mer and Axl are expressed by microglia in the adult CNS, and mediate the clearance of apoptotic cells from the niche.
- Lawrence Fourgeaud
- , Paqui G. Través
- & Greg Lemke
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Letter |
Visualization of an endogenous retinoic acid gradient across embryonic development
Genetically encoded probes for the non-peptidic morphogen retinoic acid allow the quantitative measurement of physiological RA concentration in vivo; the results support the source–sink diffusion model of morphogen dynamics proposed by Francis Crick in 1970.
- Satoshi Shimozono
- , Tadahiro Iimura
- & Atsushi Miyawaki
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Research Highlights |
A peek at organelles in live cells
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News |
Deconstructing dental plaque
Imaging technique could show how dozens of bacteria interact in the human mouth.
- Monya Baker
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News & Views |
Pull-down for single molecules
An innovative marriage of techniques, combining the principles of common protein pull-down assays with single-molecule fluorescence microscopy, opens up new ways of visualizing cellular protein complexes. See Article p.484
- Philip Tinnefeld
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Research Highlights |
Biochemistry: Picture protein