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RNA nucleation by MSL2 induces selective X chromosome compartmentalization
Dosage compensation in Drosophila involves nucleation of the dosage compensation complex at the X chromosome by MSL2 and the non-coding RNA roX.
- Claudia Isabelle Keller Valsecchi
- , M. Felicia Basilicata
- & Asifa Akhtar
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Article |
SPEN integrates transcriptional and epigenetic control of X-inactivation
The transcriptional repressor SPEN bridges the non-coding RNA Xist to transcription machinery, histone deacetylases and chromatin remodelling factors to initiate X-chromosome inactivation.
- François Dossin
- , Inês Pinheiro
- & Edith Heard
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Letter
| Open AccessLandscape of X chromosome inactivation across human tissues
Multiple transcriptome approaches, including single-cell sequencing, demonstrate that escape from X chromosome inactivation is widespread and occasionally variable between cells, chromosomes, and tissues, resulting in sex-biased expression of at least 60 genes and potentially contributing to sex-specific differences in health and disease.
- Taru Tukiainen
- , Alexandra-Chloé Villani
- & Daniel G. MacArthur
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Letter |
PionX sites mark the X chromosome for dosage compensation
Recognition of the X chromosome by the dosage compensation complex in Drosophila relies on the sequence and shape of PionX sites.
- Raffaella Villa
- , Tamas Schauer
- & Peter B. Becker
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Letter |
Structural organization of the inactive X chromosome in the mouse
An in-depth analysis of the structure, chromatin accessibility and expression status of the mouse inactive X (Xi) chromosome provides insights into the regulation of Xi chromosome structure, its dependence on the macrosatellite DXZ4 region, the Xist non-coding RNA, as well as the basis for topologically associating domain (TAD) formation on the Xi.
- Luca Giorgetti
- , Bryan R. Lajoie
- & Job Dekker
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Letter |
Condensin-driven remodelling of X chromosome topology during dosage compensation
Genome-wide chromosome conformation capture analysis in C. elegans reveals that the dosage compensation complex, a condensin complex, remodels the X chromosomes of hermaphrodites into a sex-specific topology distinct from autosomes while regulating gene expression chromosome-wide.
- Emily Crane
- , Qian Bian
- & Barbara J. Meyer
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Letter |
The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3
The mechanisms by which Xist, a long non-coding RNA, silences one X chromosome in female mammals are unknown; here a mass spectrometry-based approach is developed to identify several proteins that interact directly with Xist, including the transcriptional repressor SHARP that is required for transcriptional silencing through the histone deacetylase HDAC3.
- Colleen A. McHugh
- , Chun-Kan Chen
- & Mitchell Guttman
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Letter |
Rsx is a metatherian RNA with Xist-like properties in X-chromosome inactivation
A non-coding RNA termed Rsx, which has properties consistent with a role in X-chromosome inactivation, is identified in the marsupial Monodelphis domestica.
- Jennifer Grant
- , Shantha K. Mahadevaiah
- & James M. A. Turner
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Letter |
Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development
- Ikuhiro Okamoto
- , Catherine Patrat
- & Edith Heard
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Letter |
X chromosome dosage compensation via enhanced transcriptional elongation in Drosophila
Different organisms use a variety of mechanisms to compensate for X chromosome dosage imbalance between the sexes. In Drosophila, the MSL complex increases transcription on the single X chromosome of males and is thought to regulate transcription elongation, although mechanistic details have been unclear. Here, a global run-on sequencing technique is used to reveal that the MSL complex seems to enhance transcription by facilitating the progression of RNA polymerase II across the bodies of active X linked genes. In this way, MSL can impose dosage compensation on diverse genes with a wide range of transcription levels along the X chromosome.
- Erica Larschan
- , Eric P. Bishop
- & Mitzi I. Kuroda