Featured
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Article
| Open AccessMetformin induces lipogenic differentiation in myofibroblasts to reverse lung fibrosis
Idiopathic pulmonary fibrosis is associated with myofibroblast activation in the lungs and metabolic alterations. Here, the authors show that the antidiabetic drug metformin has antifibrotic effects in human-derived samples and mouse models, by modulating a number of metabolic pathways to induce lipogenic transdifferentiation of myofibroblasts.
- Vahid Kheirollahi
- , Roxana M. Wasnick
- & Elie El Agha
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Article
| Open AccessBone-targeting AAV-mediated silencing of Schnurri-3 prevents bone loss in osteoporosis
The adaptor protein SHN3 suppresses new bone formation by controlling osteoblast activity. Here, the authors show that ablation of SHN3 function, either genetically or by delivering an artificial miRNA via AAV9, rescues bone loss in osteoporotic mice, and show that engineering of the AAV9 capsid improves targeting to bone
- Yeon-Suk Yang
- , Jun Xie
- & Jae-Hyuck Shim
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Article
| Open AccessSerum FHR1 binding to necrotic-type cells activates monocytic inflammasome and marks necrotic sites in vasculopathies
FHR1 is a serum protein implicated in complement regulation. Here the authors show that human FHR1 binds to necrotic cells, triggering inflammasome activation in monocytes in culture, localizes to necrotic tissue and correlates with inflammatory cytokine levels in vasculopathies.
- Sarah Irmscher
- , Silke R. Brix
- & Christine Skerka
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Article
| Open AccessAdipocyte β-arrestin-2 is essential for maintaining whole body glucose and energy homeostasis
Beta3-adrenergic receptor signaling regulates adipose tissue browning. Here, the authors show that barr2 regulates internalization of beta3-adrenergic receptors and that mice lacking barr2 in adipocytes are protected from diet-induced weight gain and metabolic complications.
- Sai P. Pydi
- , Shanu Jain
- & Jürgen Wess
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Article
| Open AccessLocus coeruleus-CA1 projections are involved in chronic depressive stress-induced hippocampal vulnerability to transient global ischaemia
Depression and transient ischaemic attacks are tightly regulated but the neural circuits underlying depression-modulated ischaemic injury are not known. Here, the authors show that the locus coeruleus-CA1 pathway is involved in depression-associated ischaemia susceptibility.
- Qian Zhang
- , Dian Xing Hu
- & Bo Tian
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Article
| Open AccessSequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
Here, the authors show that sequential treatment with long-acting slow-effective release ART and AAV9- based delivery of CRISPR-Cas9 results in undetectable levels of virus and integrated DNA in a subset of humanized HIV-1 infected mice. This proof-of-concept study suggests that HIV-1 elimination is possible.
- Prasanta K. Dash
- , Rafal Kaminski
- & Howard E. Gendelman
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Article
| Open AccessInherent biomechanical traits enable infective filariae to disseminate through collecting lymphatic vessels
Stimuli affecting migration of filaria in host tissues are unclear. Using in situ imaging, Kilarski et al. here show that universal adaptations of nematodes allow Litomosoides sigmodontis infective larvae to mechanically break into pre-collecting lymphatics and follow the direction of flow towards the lymph node.
- Witold W. Kilarski
- , Coralie Martin
- & Melody A. Swartz
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Article
| Open AccessA frequent variant in the Japanese population determines quasi-Mendelian inheritance of rare retinal ciliopathy
Genetic variants in RP1 can cause hereditary retinal degeneration (HRD). Here, in a genomic screen of 331 Japanese HRD patients, the authors identify a near-polymorphic RP1 variant that causes Mendelian HRD in trans with an Alu insertion and otherwise is associated with HRD according to a complex model of inheritance.
- Konstantinos Nikopoulos
- , Katarina Cisarova
- & Carlo Rivolta
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Article
| Open AccessThe microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression
Osteoarthritis involves whole-joint tissue degeneration. Here, the authors show that miR-204 and miR-211 in mesenchymal joint cells regulate their proliferation, catabolic and osteogenic responses, and that disease progression is ameliorated by intra-articular miR-204 delivery in mice.
- Jian Huang
- , Lan Zhao
- & Di Chen
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Article
| Open AccessA neurotoxin that specifically targets Anopheles mosquitoes
So far identified clostridial neurotoxins target vertebrates. Here, Contreras et al. isolate the clostridial-like neurotoxin PMP1 from Paraclostridium bifermentans strains and show that it selectively targets anopheline mosquitoes by targeting mosquito syntaxin.
- Estefania Contreras
- , Geoffrey Masuyer
- & Sarjeet S. Gill
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Article
| Open AccessIdentification of a potent benzoxaborole drug candidate for treating cryptosporidiosis
Cryptosporidium infection can cause severe diarrhea with limited treatment options available. Here, Lunde et al. perform a drug repositioning screen with a library of benzoxaboroles and identify AN7973 as potent inhibitor of intracellular parasite development with good efficacy in murine and neonatal dairy calf disease models.
- Christopher S. Lunde
- , Erin E. Stebbins
- & Christopher D. Huston
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Article
| Open AccessTargeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy
A number of therapeutic agents aimed at reducing pathology in Duchenne muscular dystrophy have been developed, but may have off-target effects when delivered systemically. Here, the authors express the therapeutic LIF transgene in leukocytes, and show this results in targeting to inflamed dystrophic muscle and reduced fibrosis by suppressing type 2 immunity.
- Steven S. Welc
- , Ivan Flores
- & James G. Tidball
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Article
| Open AccessTGFβ-induced degradation of TRAF3 in mesenchymal progenitor cells causes age-related osteoporosis
Increased inflammation during ageing promotes osteoporosis by activating osteoclast function and inhibiting osteoblasts. Here, the authors show that TGFβ1 release from bone matrix during ageing induces degradation of TRAF3 in mesenchymal progenitor cells, leading to reduced osteoblast differentiation and increased osteoclast formation, and suggesting that pharmacological stabilization of TRAF3 could ameliorate age-related osteoporosis.
- Jinbo Li
- , Akram Ayoub
- & Brendan F. Boyce
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Article
| Open AccessGenome-wide analysis of dental caries and periodontitis combining clinical and self-reported data
Dental caries and periodontitis are among the most common medical conditions. Here, the authors report a GWAS for measures of oral health that reveals 47 risk loci for caries, find genetic correlation with 31 other complex traits and use Mendelian randomization analyses to explore potential causal relationships.
- Dmitry Shungin
- , Simon Haworth
- & Ingegerd Johansson
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Article
| Open AccessLugdunin amplifies innate immune responses in the skin in synergy with host- and microbiota-derived factors
Lugdunin is a peptide antibiotic produced by the skin commensal Staphylococcus lugdunensis. Here, the authors show that lugdunin reduces Staphylococcus aureus colonization in human keratinocytes and mouse skin by inducing the expression of human LL-37 and recruitment of monocytes and neutrophils.
- Katharina Bitschar
- , Birgit Sauer
- & Birgit Schittek
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Article
| Open AccessIdentification of HIV transmitting CD11c+ human epidermal dendritic cells
Composition and function of immune populations at barrier surfaces is crucial for response to infection. Here, the authors identify a population of dendritic cells in human epidermis, abundant in anogenital epithelia and distinct from Langerhans cells by surface phenotype and by high capacity for HIV infection and transmission.
- Kirstie M. Bertram
- , Rachel A. Botting
- & Andrew N. Harman
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Article
| Open AccessIL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection
Here, Frisbee et al. show that hypervirulent Clostridium difficile induces IL-33 expression in the gut and IL-33 reduces mortality and morbidity via group 2 innate lymphoid cells. Furthermore, serum levels of the soluble IL-33 decoy receptor, sST2, are associated with enhanced disease severity in human C. difficile patients.
- Alyse L. Frisbee
- , Mahmoud M. Saleh
- & William A. Petri Jr.
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Article
| Open AccessP2X7 receptor induces mitochondrial failure in monocytes and compromises NLRP3 inflammasome activation during sepsis
Systemic sepsis is a potentially life-threatening illness and immunocompromised individuals are especially vulnerable. Here, using a cohort of patients with intra-abdominal origin sepsis, the authors show an important role for the NLRP3 inflammasome in establishing a host response, and NLRP3 dysfunction is a common feature of sepsis mortality.
- Juan José Martínez-García
- , Helios Martínez-Banaclocha
- & Pablo Pelegrin
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Article
| Open AccessCD46 facilitates entry and dissemination of human cytomegalovirus
Human cytomegalovirus (CMV) infects a wide range of host cells. Here, using a high throughput antibody screening platform, the authors identify the cell surface receptor CD46 to be required for CMV infection of epithelial cells and trophoblast-derived cells, the latter critical for congenital CMV infection.
- Kathryn R. Stein
- , Thomas J. Gardner
- & Domenico Tortorella
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Article
| Open AccessSMPDL3b modulates insulin receptor signaling in diabetic kidney disease
Sphingomyelin phosphodiesterase acid-like 3b (SMPDL3b) is a lipid raft enzyme known to affect membrane lipid composition. Here, Mitrofanova et al. show that increased expression of SMPDL3b in diabetes impairs insulin signaling and ceramide-1-phosphate (C1P) availability in podocytes, and that C1P supplementation protects mice from diabetic kidney disease.
- A. Mitrofanova
- , S. K. Mallela
- & A. Fornoni
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Article
| Open AccessGenomic structure and diversity of Plasmodium falciparum in Southeast Asia reveal recent parasite migration patterns
Understanding genomic variation in Plasmodium falciparum parasites and inferring migration patterns can guide malaria elimination strategies. Using genome-wide data for 1722 parasites collected from 54 districts, the authors use identity-by-descent approaches to estimate regional parasite migration and spread of artemisinin drug resistance.
- Amol C. Shetty
- , Christopher G. Jacob
- & Marie A. Onyamboko
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Article
| Open AccessXX sex chromosome complement promotes atherosclerosis in mice
Men and women differ in their risk of developing coronary artery disease, in part due to differences in their levels of sex hormones. Here, AlSiraj et al. show that the XX sex genotype regulates lipid metabolism and promotes atherosclerosis independently of sex hormones in mice.
- Yasir AlSiraj
- , Xuqi Chen
- & Lisa A. Cassis
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Article
| Open AccessDeterministic processes structure bacterial genetic communities across an urban landscape
Disease transmission is particularly complex at the human-livestock-wildlife interface. Here the authors sample E. coli from wild birds near households in Nairobi and show that antimicrobial resistance gene diversity is correlated with human and lifestock density, while virulence gene diversity is correlated with avian species richness.
- J. M. Hassell
- , M. J. Ward
- & E. M. Fèvre
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Article
| Open AccessCordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents
Radiation damage causes DNA foci to form and senescence, causing ulcers. Here, the authors show that a naturally occurring adenosine analogue, cordycepin, prevents cell senescence via an increase in AMPK/NRF2, so blocking ulcers caused by radiation on skin/intestine/tongue damage in rodents.
- Ziwen Wang
- , Zelin Chen
- & Chunmeng Shi
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Article
| Open AccessComprehensive analysis of coding variants highlights genetic complexity in developmental and epileptic encephalopathy
Many causative genes are known for epileptic or developmental and epileptic encephalopathies (EE/DEE) yet a genetic diagnosis cannot be made for many patients. Here, the authors analyse whole exome sequencing data from a Japanese case−control cohort to identify common, rare and ultra-rare coding variants associated with EE/DEE.
- Atsushi Takata
- , Mitsuko Nakashima
- & Naomichi Matsumoto
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Article
| Open AccessIntroducing risk inequality metrics in tuberculosis policy development
Failure to account for heterogeneity in TB risk can mislead model-based evaluation of proposed interventions. Here, the authors introduce a metric to estimate the distribution of risk in populations from routinely collected data and find that variation in infection acquisition is the most impactful.
- M. Gabriela M. Gomes
- , Juliane F. Oliveira
- & Christian Lienhardt
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Article
| Open AccessKidney cytosine methylation changes improve renal function decline estimation in patients with diabetic kidney disease
Patients with diabetes commonly develop diabetic kidney disease (DKD). Here Gluck et al. identify a set of probes differentially methylated in renal samples from patients with DKD, and find that inclusion of these methylation probes improves current prediction models of renal function decline.
- Caroline Gluck
- , Chengxiang Qiu
- & Katalin Susztak
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Article
| Open AccessFunctional testing of thousands of osteoarthritis-associated variants for regulatory activity
GWAS have identified risk loci for osteoarthritis (OA), but the causal variants still have to be determined. Here, the authors apply a massively-parallel reporter assay to screen 1,605 candidate SNPs in 35 OA loci, which prioritizes six SNPs in four loci, one of which, rs4730222, is characterized in more detail.
- Jason C. Klein
- , Aidan Keith
- & Jay Shendure
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Article
| Open AccessFibroadipogenic progenitors are responsible for muscle loss in limb girdle muscular dystrophy 2B
Fibroadipogenic precursor cells (FAPs) contribute to fibrosis and adipogenic replacement in muscular dystrophies. Here, the authors show that FAPs contribute to adipogenic loss in mouse models of limb girdle muscular dystrophy 2B via a mechanism dependent on expression of Annexin A2, and that this process can be prevented by its pharmacologic inhibition in mice.
- Marshall W. Hogarth
- , Aurelia Defour
- & Jyoti K. Jaiswal
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Article
| Open AccessWwp2 maintains cartilage homeostasis through regulation of Adamts5
Wwp2 is an HECT-type E3 ubiquitin ligase abundantly expressed in articular cartilage. Here, the authors show that in mice, loss of Wwp2 leads to upregulated Runx2-Adamts5 signaling in articular cartilage and development of osteoarthritis, and that disease severity is reduced by injection of Wwp2 mRNA
- Sho Mokuda
- , Ryo Nakamichi
- & Hiroshi Asahara
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Article
| Open AccessAdipose HuR protects against diet-induced obesity and insulin resistance
Human antigen R (HuR) is a RNA-binding protein. Here the authors investigate its role in adipose tissue and find that it protects mice from diet-induced obesity, prevents adipocyte hypertrophy, and promotes lipolysis, which may at least in part be due to HuR-dependent ATGL mRNA stability regulation demonstrated in-vitro.
- Jingyuan Li
- , Li Gong
- & Wencheng Zhang
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Article
| Open AccessFlexible and scalable diagnostic filtering of genomic variants using G2P with Ensembl VEP
Diagnostic filtering is an important step to analyze the functional and clinical significance of the large number of genetic variants identified from next-generation genome sequencing data. Here, the authors develop a flexible and scalable system for diagnostic filtering of genetic variants using G2P with Ensembl VEP.
- Anja Thormann
- , Mihail Halachev
- & David R. FitzPatrick
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Article
| Open AccessIndividual variability in behavior and functional networks predicts vulnerability using an animal model of PTSD
How do individual differences affect vulnerability to developing post-traumatic stress disorder (PTSD)? Here, using longitudinal testing in a rat model of PTSD, the authors show patterns of pre-trauma brain connectivity and behaviors that predict PTSD-like responses to trauma exposure.
- David Dopfel
- , Pablo D. Perez
- & Nanyin Zhang
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Article
| Open AccessInter-outbreak stability reflects the size of the susceptible pool and forecasts magnitudes of seasonal epidemics
Directly measuring the size of the susceptible population is usually unfeasible before dengue outbreaks. Here, the authors show that the stability of low-incidence periods provides a proxy measure, which can be estimated from incidence data, and show its utility for forecasting outbreaks.
- Martin Rypdal
- & George Sugihara
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Article
| Open AccessStructure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence
Stabilized, native-like trimers of the HIV envelope protein, such as SOSIP trimers, are potential antigens for an HIV vaccine. Here, the authors generate a SOSIP trimer based on the consensus sequence of group M isolates, determine its structure and exposure of common epitopes, and show immunogenicity in rabbits and non-human primates.
- Kwinten Sliepen
- , Byung Woo Han
- & Rogier W. Sanders
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Article
| Open AccessThe economics of malaria control in an age of declining aid
Foreign aid is necessary to control tropical diseases in endemic countries. Here the authors outline the steps taken to control malaria in Africa since 2000 and present an economic model to propose that US$25−30 per capita will be needed to avoid a disease trap.
- Eric Maskin
- , Célestin Monga
- & Jean-Claude Berthélemy
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Article
| Open AccessHuman mobility patterns and malaria importation on Bioko Island
Malaria on Bioko Island has been reduced substantially but many acquire malaria while traveling. Here, the authors use survey data, geostatistical and mathematical modeling to investigate malaria prevalence and mobility patterns and find that in some parts of the island a significant fraction of prevalence is attributable to malaria acquired while traveling.
- Carlos A. Guerra
- , Su Yun Kang
- & David L. Smith
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Article
| Open AccessSchistosoma mansoni treatment reduces HIV entry into cervical CD4+ T cells and induces IFN-I pathways
Schistosoma mansoniinfection has been linked with an increased risk of HIV acquisition in women. Here, the authors show that standard S. mansoniinfection treatment causes a reduction of HIV entry into cervical and blood CD4+ T cells, which is sustained for up to two months and is associated with de-repression of IFN-I signaling.
- Sergey Yegorov
- , Vineet Joag
- & Rupert Kaul
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Article
| Open AccessAltered respiratory virome and serum cytokine profile associated with recurrent respiratory tract infections in children
Here, the authors determine the respiratory virome and serum cytokine profile in children diagnosed with acute respiratory tract infections (ARTI) and show that relative abundance of Propionibacterium phages as well as serum levels of TIMP-1 and PDGF-BB are increased in multiple ARTIs compared with single ARTI.
- Yanpeng Li
- , Xuemin Fu
- & Chiyu Zhang
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Article
| Open AccessRecapitulation of HDV infection in a fully permissive hepatoma cell line allows efficient drug evaluation
Hepatitis delta virus (HDV) depends on the envelope proteins of hepatitis B virus (HBV) for virion production. Here, Lempp et al. produce a cell line expressing HBV envelope proteins and their receptor, which allows continuous secretion of infectious progeny HDV and testing of antiviral drugs.
- Florian A. Lempp
- , Franziska Schlund
- & Stephan Urban
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Article
| Open AccessZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes
Complement activation contributes to vascular inflammation in the contexts of allograft rejection and connective tissue disease. Here Fang et al. identify ZFYVE21 as a novel effector of Rab5 and find it regulates pro-inflammatory NF-κB signaling in endothelial cells in response to complement activation.
- Caodi Fang
- , Thomas D. Manes
- & Dan Jane-wit
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Article
| Open AccessInactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis
Idiopathic pulmonary fibrosis (IPF) is a lethal disease with insufficient treatment strategies. Here the authors show that reduction of the microRNA MIRLET7D and hyperactivation of EP300 contribute to impaired epigenetic silencing by the MiCEE complex in pulmonary fibroblasts of IPF patients, and demonstrate the benefit of inhibiting EP300 for the treatment of IPF.
- Karla Rubio
- , Indrabahadur Singh
- & Guillermo Barreto
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Article
| Open AccessFunctional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors
Cardiac fibroblasts (CFs) play critical roles in heart development, homeostasis, and disease. Here the authors efficiently differentiate human pluripotent stem cells through second heart field progenitors to CFs that exhibit features and functional properties similar to native CFs.
- Jianhua Zhang
- , Ran Tao
- & Timothy J. Kamp
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Article
| Open AccessTargeting cyclin-dependent kinases for the treatment of pulmonary arterial hypertension
Cells of the pulmonary vasculature show a hyperproliferative phenotype in pulmonary arterial hypertension (PAH), thus contributing to the disease pathogenesis. Here the authors show that cyclin-dependent kinases are overactivated in PAH, and that their pharmacological inhibition attenuates the disease in two independent rodent models
- Astrid Weiss
- , Moritz Christian Neubauer
- & Ralph Theo Schermuly
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Article
| Open AccessFunctional rare and low frequency variants in BLK and BANK1 contribute to human lupus
Function-altering variants of immune-related genes cause rare autoimmune syndromes, whereas their contribution to common autoimmune diseases remains uncharacterized. Here the authors show that rare variants of lupus-associated genes are present in the majority of lupus patients and healthy controls, but only the variants found in lupus patients alter gene function.
- Simon H. Jiang
- , Vicki Athanasopoulos
- & Carola G. Vinuesa
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Article
| Open AccessInnate lymphocyte-induced CXCR3B-mediated melanocyte apoptosis is a potential initiator of T-cell autoreactivity in vitiligo
Tissue signals that prime autoreactive T cells at the onset of autoimmunity remain enigmatic. Here the authors show NK and ILC1 cells are increased in vitiligo patients, and induce melanocyte apoptosis via CXCR3B, which in turn leads to increased priming of T cell responses in cell culture.
- Meri K. Tulic
- , Elisa Cavazza
- & Thierry Passeron
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Article
| Open AccessJoint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis
Streptococcus pneumoniae is a causative agent of meningitis and bacteremia. In a combined pathogen and host GWAS, Lees et al. find that host genetic variation is associated with both susceptibility and severity of pneumococcal meningitis, and specific bacterial genetic variation associated with susceptibility.
- John A. Lees
- , Bart Ferwerda
- & Diederik van de Beek
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Article
| Open AccessInterferon inducible X-linked gene CXorf21 may contribute to sexual dimorphism in Systemic Lupus Erythematosus
Systemic lupus erythematosus (SLE) shows a striking bias towards higher prevalence in females. Here, the authors perform fine-mapping of an SLE-associated locus at Xp21.2 and characterise a candidate gene, CXorf21, as IFN-responsive in immune cells that shows sexually dimorphic expression.
- Christopher A. Odhams
- , Amy L. Roberts
- & Timothy J. Vyse
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Article
| Open AccessPreclinical safety study of a combined therapeutic bone wound dressing for osteoarticular regeneration
Arthroplasty is the main clinical option for the treatment of osteoarticular lesions, but has limited efficacy. Here, the authors use a wound dressing with autologous mesenchymal stromal cells, functionalised for local BMP2 delivery, and show feasibility and safety in standardised preclinical tests in animal models, suggesting suitability for use in clinical trials.
- Laetitia Keller
- , Luc Pijnenburg
- & Nadia Benkirane-Jessel
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